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The map label for this gene is speE [H]

Identifier: 94313452

GI number: 94313452

Start: 1131915

End: 1134449

Strand: Reverse

Name: speE [H]

Synonym: Rmet_4527

Alternate gene names: 94313452

Gene position: 1134449-1131915 (Counterclockwise)

Preceding gene: 94313453

Following gene: 94313451

Centisome position: 43.97

GC content: 67.97

Gene sequence:

>2535_bases
ATGCGTGCGAAACGGCGGCCAGCCCAAGCATCCGAGCCTGCAGCCGTGCCGCGGTCGACCGCATGGCGATGGCCCGCGTT
GCTGCTGATCCTGTCCGGCGCCGCGGGCCTGGTTTATCAGGTGCTGTGGATCAAGCAGCTTTCGCTGGTGGTCGGCGTCG
AGGTGCAGGCGGTGACCACCGGTGTCAGCGCGTTCTTCGCGGGCCTGGCGCTTGGCGGCTGGCTGGTTGGCCGTCACGCC
GATCGCAGTACCTCCGCCTGGCGGCTATACCTCTGGCTCGAAGTCGCCATTGCCTTGCTGGGCATTGGCGCGACGCTGGC
GCTGGCTCATGCGGCGGCGCCGTTCGCATGGCTGCAGTTGCGCGCGGGGGCCGTGGCGTGGGCGCTTCCGGTGCTGCTGG
TCGGCCTGCCCGCGGTCGCAATGGGCGGCACGCTGCCGGCGCTGCTCCGGGCGGTGACGCCCGCCGAGCACGAACTCGGT
GGCCACGGCGGCCGGTTCTACGCGGCCAACACGGCCGGTGCCATTGCGGGCACGCTGCTCGCGGGGTTTGTGTGGATTCC
TGCGTTCGGCGTCACGGGTAGCGCCTGCGCGGCAGCCGCGCTGAACCTGCTGGCCGCACTCGGCGCGTACTGGATGGTCC
GCCGCGCCCCCAAAGCGACCACCCAGACCGTGCCACAGGCGCAAGTCGAGGCAACGCCAAGGACTGGGCAGCGCACACTG
GCGCTCACGCTTTATGCCTTGGCCGGCGGTATCGCGCTTGGCTACGAGGTGGTCTGGTCGCAAGCCATTGTCCAGTTCCT
GAGCACGCGCGCATTCGCGTTCGCGGTGATGCTTGCCACGTATCTGGCGGGCCTCATGCTTGGCAGTGCGCTGGTGGCAC
GCAAGGTCGATCGAGCCAGGGACCCTTGGGGTGTGTTCGCGGCGCTGGTTGTCGGCGCGGGGCTGGCGGGTCTGGTCGAA
TTCGCATGGCTGGGTGAATGGCTCCCGGCCGCCCAGTCCGTGGTGTCACAACGCGTGCTCGGCGCCACCGGCAGCCAGCT
TGCCGCCATGTGCGCGAGCTTTGGCATGGCGGCATTTTGTATTGTGTTCGTGCCGACGGTGCTGCTTGGCGCGGCGTTTC
CGTATGTCGTGCGACTCTCCGTCGATGTCTCGCGCCCCGGTTCAGGGCTCGGCGCCGTGCTTGCAGTGAATACGCTGGGC
GGTATTGCTGGCTCGGTGCTGGCCGGCTTCGTGCTGGTGCCGTGGATCGGCGTGGTCCATACGCTCGGCGTGCTGGCCGT
GGCGAGCGGCGTCGTGGCGCTGCTGGCTACCGCATTCGGCACCGGCGTGCACCGCTTCGCACGGGTGGCCGTGCCGTTGC
TGGCCGTGGTGACCTTGATCGTGGCCGTGGTGACGCCGGCCGATCGGCTTGCCGCATTGCTGGCGCAGACGCGCGGCGGT
GAACTTGTTTTCTACGAGGAAAGCCGCGGCGCCACGGTAGCCGTGGTCGAGCAGGGCAAGGCCCGTCAACCGTTCCGGCG
GCTCTATATTCAGGGCGTATCGAATTCCGGCGATACGATGACGTCGCTGCGCTATATGCGGCTGCAGGCGCTGCTGCCCC
TGCTGATCCACAACGGCGCACCGCGCTCCACCCTGGTGATCGGCCTTGGGACCGGTATTACCGCAGGCGCGCTGCTGCAG
TATCCGGGGCTCGAGCATCGGGTAGTGGCCGAGTTGCTGCCCGCCGTGGTGCGTGCCGCGCCGAACTTCCAGGGTAACTA
CGGCGTGACTGGCGACAAGCGCGTCGACATCCGCTTGCGCGACGGTCGGCGCGAGCTGCTGCAAAGCGCCCAGCGTTACG
ACCTGATCACGCTGGAACCGCCGCCGCCGTCGGCCAGCGGTGTCGTGAACCTGTATTCGCGCGATTTCTATCGTCTCGCC
GCGCAGCGTCTGGAGACGGGCGGCATTGTGGCGCAATGGCTGCCCCTGCCGACGCAGAATCCCGAGGACACCCGCTCGCT
GGTGCGTAGCTTCCTCGATGTGTTTCCGCACGCCACGCTCTGGACCACCGAACTGCACGAGATGCTGTTGGTTGGATCAT
TGCAGCCGGTCACGCTTGACGTGGCGCGTATTCAGTCGCGTTTCGCCGATGCCGGGGTCAGCGCCGCGCTGCGGTCCGTC
GGCGTGGCATCGCCCGCCGCCTTGCTGGCCACCTACGTGACCGACCGCACCGGCCTCGACTGGTTTGCCAGCGACGCGCC
CGCCGTTACAGACGACCGTCCGCGCATCGAATACTCGGGCTGGGTGCGCAAGCAGTCGTTCCCGCCCGTGCTGGAGGAGT
TGCTGAATCTCTCGTCCGAGCCGCAACTGCAAGGCGCGGACGACGCACTGCAAGTCGCCATCCGGCGCGAGCGGGCCAAC
CTGCATCTGTTCTATCGCGCCGGGCTCGATGCATACCGGGGCGACCGCCAGCAATGGCTGCGCGATATCACGCAGGTGAT
GCGCGAGGATCCGGGCAATCCGTACTACAACTGGTTCACGGGAGGCGCGCAATGA

Upstream 100 bases:

>100_bases
CCACAACACCAACAATAGCGGAGCCCTCGATTGGCAGACTTCACAGGCAACAAGCGCGGCAAGAAACGTGACGGCAGCCG
ACCGTAAGCTGGGGCAGGGG

Downstream 100 bases:

>100_bases
GCGCTCGCGACGATTTTCTGGCGCTGCAGGCACGTATGGGCGAAACCATTGTCGGCCAGGCACGCATGATCGAGCGGTTG
TTGCTTGGGCTGCTTGCCGA

Product: Spermine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 844; Mature: 844

Protein sequence:

>844_residues
MRAKRRPAQASEPAAVPRSTAWRWPALLLILSGAAGLVYQVLWIKQLSLVVGVEVQAVTTGVSAFFAGLALGGWLVGRHA
DRSTSAWRLYLWLEVAIALLGIGATLALAHAAAPFAWLQLRAGAVAWALPVLLVGLPAVAMGGTLPALLRAVTPAEHELG
GHGGRFYAANTAGAIAGTLLAGFVWIPAFGVTGSACAAAALNLLAALGAYWMVRRAPKATTQTVPQAQVEATPRTGQRTL
ALTLYALAGGIALGYEVVWSQAIVQFLSTRAFAFAVMLATYLAGLMLGSALVARKVDRARDPWGVFAALVVGAGLAGLVE
FAWLGEWLPAAQSVVSQRVLGATGSQLAAMCASFGMAAFCIVFVPTVLLGAAFPYVVRLSVDVSRPGSGLGAVLAVNTLG
GIAGSVLAGFVLVPWIGVVHTLGVLAVASGVVALLATAFGTGVHRFARVAVPLLAVVTLIVAVVTPADRLAALLAQTRGG
ELVFYEESRGATVAVVEQGKARQPFRRLYIQGVSNSGDTMTSLRYMRLQALLPLLIHNGAPRSTLVIGLGTGITAGALLQ
YPGLEHRVVAELLPAVVRAAPNFQGNYGVTGDKRVDIRLRDGRRELLQSAQRYDLITLEPPPPSASGVVNLYSRDFYRLA
AQRLETGGIVAQWLPLPTQNPEDTRSLVRSFLDVFPHATLWTTELHEMLLVGSLQPVTLDVARIQSRFADAGVSAALRSV
GVASPAALLATYVTDRTGLDWFASDAPAVTDDRPRIEYSGWVRKQSFPPVLEELLNLSSEPQLQGADDALQVAIRRERAN
LHLFYRAGLDAYRGDRQQWLRDITQVMREDPGNPYYNWFTGGAQ

Sequences:

>Translated_844_residues
MRAKRRPAQASEPAAVPRSTAWRWPALLLILSGAAGLVYQVLWIKQLSLVVGVEVQAVTTGVSAFFAGLALGGWLVGRHA
DRSTSAWRLYLWLEVAIALLGIGATLALAHAAAPFAWLQLRAGAVAWALPVLLVGLPAVAMGGTLPALLRAVTPAEHELG
GHGGRFYAANTAGAIAGTLLAGFVWIPAFGVTGSACAAAALNLLAALGAYWMVRRAPKATTQTVPQAQVEATPRTGQRTL
ALTLYALAGGIALGYEVVWSQAIVQFLSTRAFAFAVMLATYLAGLMLGSALVARKVDRARDPWGVFAALVVGAGLAGLVE
FAWLGEWLPAAQSVVSQRVLGATGSQLAAMCASFGMAAFCIVFVPTVLLGAAFPYVVRLSVDVSRPGSGLGAVLAVNTLG
GIAGSVLAGFVLVPWIGVVHTLGVLAVASGVVALLATAFGTGVHRFARVAVPLLAVVTLIVAVVTPADRLAALLAQTRGG
ELVFYEESRGATVAVVEQGKARQPFRRLYIQGVSNSGDTMTSLRYMRLQALLPLLIHNGAPRSTLVIGLGTGITAGALLQ
YPGLEHRVVAELLPAVVRAAPNFQGNYGVTGDKRVDIRLRDGRRELLQSAQRYDLITLEPPPPSASGVVNLYSRDFYRLA
AQRLETGGIVAQWLPLPTQNPEDTRSLVRSFLDVFPHATLWTTELHEMLLVGSLQPVTLDVARIQSRFADAGVSAALRSV
GVASPAALLATYVTDRTGLDWFASDAPAVTDDRPRIEYSGWVRKQSFPPVLEELLNLSSEPQLQGADDALQVAIRRERAN
LHLFYRAGLDAYRGDRQQWLRDITQVMREDPGNPYYNWFTGGAQ
>Mature_844_residues
MRAKRRPAQASEPAAVPRSTAWRWPALLLILSGAAGLVYQVLWIKQLSLVVGVEVQAVTTGVSAFFAGLALGGWLVGRHA
DRSTSAWRLYLWLEVAIALLGIGATLALAHAAAPFAWLQLRAGAVAWALPVLLVGLPAVAMGGTLPALLRAVTPAEHELG
GHGGRFYAANTAGAIAGTLLAGFVWIPAFGVTGSACAAAALNLLAALGAYWMVRRAPKATTQTVPQAQVEATPRTGQRTL
ALTLYALAGGIALGYEVVWSQAIVQFLSTRAFAFAVMLATYLAGLMLGSALVARKVDRARDPWGVFAALVVGAGLAGLVE
FAWLGEWLPAAQSVVSQRVLGATGSQLAAMCASFGMAAFCIVFVPTVLLGAAFPYVVRLSVDVSRPGSGLGAVLAVNTLG
GIAGSVLAGFVLVPWIGVVHTLGVLAVASGVVALLATAFGTGVHRFARVAVPLLAVVTLIVAVVTPADRLAALLAQTRGG
ELVFYEESRGATVAVVEQGKARQPFRRLYIQGVSNSGDTMTSLRYMRLQALLPLLIHNGAPRSTLVIGLGTGITAGALLQ
YPGLEHRVVAELLPAVVRAAPNFQGNYGVTGDKRVDIRLRDGRRELLQSAQRYDLITLEPPPPSASGVVNLYSRDFYRLA
AQRLETGGIVAQWLPLPTQNPEDTRSLVRSFLDVFPHATLWTTELHEMLLVGSLQPVTLDVARIQSRFADAGVSAALRSV
GVASPAALLATYVTDRTGLDWFASDAPAVTDDRPRIEYSGWVRKQSFPPVLEELLNLSSEPQLQGADDALQVAIRRERAN
LHLFYRAGLDAYRGDRQQWLRDITQVMREDPGNPYYNWFTGGAQ

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG4262

COG function: function code R; Predicted spermidine synthase with an N-terminal membrane domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 89585; Mature: 89585

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAKRRPAQASEPAAVPRSTAWRWPALLLILSGAAGLVYQVLWIKQLSLVVGVEVQAVTT
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHH
GVSAFFAGLALGGWLVGRHADRSTSAWRLYLWLEVAIALLGIGATLALAHAAAPFAWLQL
HHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
RAGAVAWALPVLLVGLPAVAMGGTLPALLRAVTPAEHELGGHGGRFYAANTAGAIAGTLL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHCCCCCCEEEECCCHHHHHHHHH
AGFVWIPAFGVTGSACAAAALNLLAALGAYWMVRRAPKATTQTVPQAQVEATPRTGQRTL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHCCCCCCCCCHHH
ALTLYALAGGIALGYEVVWSQAIVQFLSTRAFAFAVMLATYLAGLMLGSALVARKVDRAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DPWGVFAALVVGAGLAGLVEFAWLGEWLPAAQSVVSQRVLGATGSQLAAMCASFGMAAFC
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
IVFVPTVLLGAAFPYVVRLSVDVSRPGSGLGAVLAVNTLGGIAGSVLAGFVLVPWIGVVH
HHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLGVLAVASGVVALLATAFGTGVHRFARVAVPLLAVVTLIVAVVTPADRLAALLAQTRGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
ELVFYEESRGATVAVVEQGKARQPFRRLYIQGVSNSGDTMTSLRYMRLQALLPLLIHNGA
EEEEEECCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
PRSTLVIGLGTGITAGALLQYPGLEHRVVAELLPAVVRAAPNFQGNYGVTGDKRVDIRLR
CCCEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEC
DGRRELLQSAQRYDLITLEPPPPSASGVVNLYSRDFYRLAAQRLETGGIVAQWLPLPTQN
CCHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
PEDTRSLVRSFLDVFPHATLWTTELHEMLLVGSLQPVTLDVARIQSRFADAGVSAALRSV
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GVASPAALLATYVTDRTGLDWFASDAPAVTDDRPRIEYSGWVRKQSFPPVLEELLNLSSE
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCC
PQLQGADDALQVAIRRERANLHLFYRAGLDAYRGDRQQWLRDITQVMREDPGNPYYNWFT
CCCCCCCHHHHHHHHHHCCCEEEEEECCCHHHCCCHHHHHHHHHHHHHCCCCCCCEECCC
GGAQ
CCCC
>Mature Secondary Structure
MRAKRRPAQASEPAAVPRSTAWRWPALLLILSGAAGLVYQVLWIKQLSLVVGVEVQAVTT
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHH
GVSAFFAGLALGGWLVGRHADRSTSAWRLYLWLEVAIALLGIGATLALAHAAAPFAWLQL
HHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
RAGAVAWALPVLLVGLPAVAMGGTLPALLRAVTPAEHELGGHGGRFYAANTAGAIAGTLL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHCCCCCCEEEECCCHHHHHHHHH
AGFVWIPAFGVTGSACAAAALNLLAALGAYWMVRRAPKATTQTVPQAQVEATPRTGQRTL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHCCCCCCCCCHHH
ALTLYALAGGIALGYEVVWSQAIVQFLSTRAFAFAVMLATYLAGLMLGSALVARKVDRAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DPWGVFAALVVGAGLAGLVEFAWLGEWLPAAQSVVSQRVLGATGSQLAAMCASFGMAAFC
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
IVFVPTVLLGAAFPYVVRLSVDVSRPGSGLGAVLAVNTLGGIAGSVLAGFVLVPWIGVVH
HHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLGVLAVASGVVALLATAFGTGVHRFARVAVPLLAVVTLIVAVVTPADRLAALLAQTRGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
ELVFYEESRGATVAVVEQGKARQPFRRLYIQGVSNSGDTMTSLRYMRLQALLPLLIHNGA
EEEEEECCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
PRSTLVIGLGTGITAGALLQYPGLEHRVVAELLPAVVRAAPNFQGNYGVTGDKRVDIRLR
CCCEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEC
DGRRELLQSAQRYDLITLEPPPPSASGVVNLYSRDFYRLAAQRLETGGIVAQWLPLPTQN
CCHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
PEDTRSLVRSFLDVFPHATLWTTELHEMLLVGSLQPVTLDVARIQSRFADAGVSAALRSV
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GVASPAALLATYVTDRTGLDWFASDAPAVTDDRPRIEYSGWVRKQSFPPVLEELLNLSSE
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCC
PQLQGADDALQVAIRRERANLHLFYRAGLDAYRGDRQQWLRDITQVMREDPGNPYYNWFT
CCCCCCCHHHHHHHHHHCCCEEEEEECCCHHHCCCHHHHHHHHHHHHHCCCCCCCEECCC
GGAQ
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]