Click here to switch to the map view.

The map label for this gene is rpoJ [H]

Identifier: 94313424

GI number: 94313424

Start: 1100615

End: 1101115

Strand: Reverse

Name: rpoJ [H]

Synonym: Rmet_4499

Alternate gene names: 94313424

Gene position: 1101115-1100615 (Counterclockwise)

Preceding gene: 94313426

Following gene: 94313423

Centisome position: 42.68

GC content: 61.28

Gene sequence:

>501_bases
ATGGATCCAGGTACCCGTTCGCAGGTCGATGTCCTTTACAGCCGGCATCACGCATGGCTGCAAGGCTGGCTGTTGCGAAA
GATCGGCAATGCATGCGATGCGGCGGACATCGCCCAGGATGTATTCGTCAGGGTGCTTCACAGGAAAGTCGTCGAGATCC
GTGAGCCGCGCACCTACCTGGGCACCATTGCCAATGGGCTGGTCATCGATTTCTGGCGGCGGCGGGAACTTGAGCAAGCA
TGGCTGGAGACGCTGGCTCAATTGCCGGAAGCACATACGCCATCGCCCGAAAGCCGTCTGCACTTCCTGCAGACGTTGAT
CGAGATCGATCAGATGCTCGATACGCTGAAGCCGAACGCGCGCACGGCATTCCTGCTGGCGCAGCTCGACGGCCTGACTT
GCCCGCAGATCGCGGAGCGCATTGGCATGTCGCTGGCGACCGTCGAGCGGTACATCAGCCAGGCGCTGCGCCGATGCTAC
GCGTTGCAGTTCGGCGCTTAG

Upstream 100 bases:

>100_bases
GGGATCTCAATCAGCTCGTCCATAGCGGGGCGCCGTCAGTTGGCGGCGTATGTCCTGGAAGACCTGATGACACCCGCTGC
GGTGAGATGAGGTAGTCGTG

Downstream 100 bases:

>100_bases
CAACCGGCACAAGCACAGGGCGCAAAGATGTCCAGTTCGCAAGCCGATTTGCCGTCAGAAGTCATTGATGCGGCCATTGA
TTGGGCCATCCGCCTGCTGT

Product: RNA polymerase sigma J factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MDPGTRSQVDVLYSRHHAWLQGWLLRKIGNACDAADIAQDVFVRVLHRKVVEIREPRTYLGTIANGLVIDFWRRRELEQA
WLETLAQLPEAHTPSPESRLHFLQTLIEIDQMLDTLKPNARTAFLLAQLDGLTCPQIAERIGMSLATVERYISQALRRCY
ALQFGA

Sequences:

>Translated_166_residues
MDPGTRSQVDVLYSRHHAWLQGWLLRKIGNACDAADIAQDVFVRVLHRKVVEIREPRTYLGTIANGLVIDFWRRRELEQA
WLETLAQLPEAHTPSPESRLHFLQTLIEIDQMLDTLKPNARTAFLLAQLDGLTCPQIAERIGMSLATVERYISQALRRCY
ALQFGA
>Mature_166_residues
MDPGTRSQVDVLYSRHHAWLQGWLLRKIGNACDAADIAQDVFVRVLHRKVVEIREPRTYLGTIANGLVIDFWRRRELEQA
WLETLAQLPEAHTPSPESRLHFLQTLIEIDQMLDTLKPNARTAFLLAQLDGLTCPQIAERIGMSLATVERYISQALRRCY
ALQFGA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates the fec genes for iron dicitrate transport (Probable) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790746, Length=159, Percent_Identity=47.1698113207547, Blast_Score=156, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 19007; Mature: 19007

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPGTRSQVDVLYSRHHAWLQGWLLRKIGNACDAADIAQDVFVRVLHRKVVEIREPRTYL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
GTIANGLVIDFWRRRELEQAWLETLAQLPEAHTPSPESRLHFLQTLIEIDQMLDTLKPNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
RTAFLLAQLDGLTCPQIAERIGMSLATVERYISQALRRCYALQFGA
HHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDPGTRSQVDVLYSRHHAWLQGWLLRKIGNACDAADIAQDVFVRVLHRKVVEIREPRTYL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
GTIANGLVIDFWRRRELEQAWLETLAQLPEAHTPSPESRLHFLQTLIEIDQMLDTLKPNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
RTAFLLAQLDGLTCPQIAERIGMSLATVERYISQALRRCYALQFGA
HHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2254251; 7610040; 9278503; 8052622 [H]