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The map label for this gene is tnpR [H]

Identifier: 94313398

GI number: 94313398

Start: 1071617

End: 1072177

Strand: Direct

Name: tnpR [H]

Synonym: Rmet_4473

Alternate gene names: 94313398

Gene position: 1071617-1072177 (Clockwise)

Preceding gene: 94313392

Following gene: 94313399

Centisome position: 41.53

GC content: 62.03

Gene sequence:

>561_bases
TTGCACGGTCAGCGCATCGGTTACGTCCGCGTCAGCAGCTTCGACCAGAACCCAGAACGGCAGCTCGAACAGATCCAGGT
GGATAAAGTGTTCACCGACAAGGCGTCGGGCAAGGACACACGGCGGCCCGAACTGGAACGGCTGCTCGCCTTCGTGCGCG
AAGGCGACACGGTCGTGGTGCACAGCATGGATCGTCTGGCGCGCAACCTCGACGACCTGCGCCGCCTGGTGCAGGGCCTC
ACCCAGCGCGGCGTACGCATCGAGTTCCTTAAGGAGCATTTGACCTTCACCGGCGAGGACTCGCCGATGGCGAACCTGAT
GCTGTCGGTAATGGGCGCGTTCGCCGAGTTCGAACGCGCCTTGATCCGCGAGCGGCAGCGCGAGGGCATCGCGCTCGCCA
AGCAGCGCGGGGCCTACCGTGGCAGGAAGAAATCCCTGTCGTCTGAGCGTATTGCCGAACTGCGCCAACGTGTCGAGGCT
GGCGAGCAAAAGACCAAGCTGGCTCGTGAATTCGGAATCAGTCGCGAAACCCTGTATCAATACTTGAGAACGGATCAGTA
A

Upstream 100 bases:

>100_bases
GGCGCTAAATCGTCAGAATAGAGTTGCCTTCCGAATTGATTGACATACGCCGTCAAGGGTCATAGATTTCTTCCTGACAC
ATTTCCCTCAGGAGGATACC

Downstream 100 bases:

>100_bases
ATATGCCACGTCGTTCAATCCTGTCCGCCGCCGAGCGCGAAAGCCTGCTGGCGTTGCCGGACACCAAGGATGAGTTGATC
CGTCACTACACGTTCAGCGA

Product: serine-based site-specific recombinase activity TnpR (resolvase) Tn6050

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MHGQRIGYVRVSSFDQNPERQLEQIQVDKVFTDKASGKDTRRPELERLLAFVREGDTVVVHSMDRLARNLDDLRRLVQGL
TQRGVRIEFLKEHLTFTGEDSPMANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
GEQKTKLAREFGISRETLYQYLRTDQ

Sequences:

>Translated_186_residues
MHGQRIGYVRVSSFDQNPERQLEQIQVDKVFTDKASGKDTRRPELERLLAFVREGDTVVVHSMDRLARNLDDLRRLVQGL
TQRGVRIEFLKEHLTFTGEDSPMANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
GEQKTKLAREFGISRETLYQYLRTDQ
>Mature_186_residues
MHGQRIGYVRVSSFDQNPERQLEQIQVDKVFTDKASGKDTRRPELERLLAFVREGDTVVVHSMDRLARNLDDLRRLVQGL
TQRGVRIEFLKEHLTFTGEDSPMANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
GEQKTKLAREFGISRETLYQYLRTDQ

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=178, Percent_Identity=37.6404494382022, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1787827, Length=186, Percent_Identity=34.9462365591398, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1787638, Length=183, Percent_Identity=34.9726775956284, Blast_Score=99, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 21596; Mature: 21596

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGQRIGYVRVSSFDQNPERQLEQIQVDKVFTDKASGKDTRRPELERLLAFVREGDTVVV
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEEEE
HSMDRLARNLDDLRRLVQGLTQRGVRIEFLKEHLTFTGEDSPMANLMLSVMGAFAEFERA
ECHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEAGEQKTKLAREFGISRETLYQ
HHHHHHHCCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHH
YLRTDQ
HHHCCC
>Mature Secondary Structure
MHGQRIGYVRVSSFDQNPERQLEQIQVDKVFTDKASGKDTRRPELERLLAFVREGDTVVV
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEEEE
HSMDRLARNLDDLRRLVQGLTQRGVRIEFLKEHLTFTGEDSPMANLMLSVMGAFAEFERA
ECHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEAGEQKTKLAREFGISRETLYQ
HHHHHHHCCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHH
YLRTDQ
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2992807; 6312271; 3029727 [H]