Click here to switch to the map view.

The map label for this gene is nit [C]

Identifier: 94313360

GI number: 94313360

Start: 1030745

End: 1031581

Strand: Reverse

Name: nit [C]

Synonym: Rmet_4435

Alternate gene names: 94313360

Gene position: 1031581-1030745 (Counterclockwise)

Preceding gene: 94313361

Following gene: 94313357

Centisome position: 39.98

GC content: 66.55

Gene sequence:

>837_bases
ATGATGCGGATAAAAGTCGCTGCCATCCAGATCGACAGCCGGCAGGACCGCGAGGCTAACCTGGCGGCACTCGAACACTG
GATTCTTGCCGCCGCGTCGGACGGCGCGAAACTGATCGTTACGCCGGAGTACAGCGATGTCCGCGGCGATGCCAACGCGC
TACAGGCGGCCGCCAGCGCGGTTCCGGGCCCGGTCTCCGAGCACATCTCCTCGCTGGCGCAACGCACCGGTTGCTGGATT
CATCTGGGCTCGATGCATGAACGCCTGCCAGGCGAGACCCGCCTCGGCAATAGCGGCATCACGTTCGCGCCAGATGGTGG
CATTGCCGCACGCTATCGCAAGGTGCACCTGTACGACGCCGTGGTGAATGGCAAACCCTATCGCGAGTCAGCCGACTTCG
CGCCGGGGGACGGCCTGCATACCGTGGACGCTGCCGGCCTGACCCTGGGTCTGAGCATCTGCTACGACCTGCGCTTCGGC
GAGCTGTACCGGACCCTGCGCGCACGCGGCGCCAACGTGCTGCTGGTGCCCGCCGCTTTTAACGTGCACACCGGACGCGA
CCACTGGGAGACGCTGCTGCGGGCCCGTGCCATCGAAAACCAGTGCTACGTGATTGCCGCCGCGCAGATCGGCGGCCCGG
GACCCGCCCTGCCCTGCCTGGGGCGCAGCATGATCATTGACCCCTGGGGCACCGTGCTGGCCTGTATGCCGGACACCACC
GGCTTCATCACCGCCGAACTCGATACAGAGCGCGTGGCCGGCCTGCGCGAGGCGCTTCCGGCATGGGAGCATCGACGCAC
GGATCTGTACTTCGGCGGCATTCAGAACTCAGGCTGA

Upstream 100 bases:

>100_bases
CGCAGACGCTGCACGGTGGCGATGGCGCGCAATGGCTGGCCGAAGCGGCGCGGATCGTGGCGCAGGCGCTTGCCGCGCAG
CAAGACGAGAACGGAGCGGA

Downstream 100 bases:

>100_bases
GCCCTGGCCCCGCGTCCAAGAGACGCCATCAAATTGGGGCGAAGCGGCTCTGGCTGAACGCCCGGCTGCGGACAGTACAA
GTACGCCAGAATCGCTTTGA

Product: Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MMRIKVAAIQIDSRQDREANLAALEHWILAAASDGAKLIVTPEYSDVRGDANALQAAASAVPGPVSEHISSLAQRTGCWI
HLGSMHERLPGETRLGNSGITFAPDGGIAARYRKVHLYDAVVNGKPYRESADFAPGDGLHTVDAAGLTLGLSICYDLRFG
ELYRTLRARGANVLLVPAAFNVHTGRDHWETLLRARAIENQCYVIAAAQIGGPGPALPCLGRSMIIDPWGTVLACMPDTT
GFITAELDTERVAGLREALPAWEHRRTDLYFGGIQNSG

Sequences:

>Translated_278_residues
MMRIKVAAIQIDSRQDREANLAALEHWILAAASDGAKLIVTPEYSDVRGDANALQAAASAVPGPVSEHISSLAQRTGCWI
HLGSMHERLPGETRLGNSGITFAPDGGIAARYRKVHLYDAVVNGKPYRESADFAPGDGLHTVDAAGLTLGLSICYDLRFG
ELYRTLRARGANVLLVPAAFNVHTGRDHWETLLRARAIENQCYVIAAAQIGGPGPALPCLGRSMIIDPWGTVLACMPDTT
GFITAELDTERVAGLREALPAWEHRRTDLYFGGIQNSG
>Mature_278_residues
MMRIKVAAIQIDSRQDREANLAALEHWILAAASDGAKLIVTPEYSDVRGDANALQAAASAVPGPVSEHISSLAQRTGCWI
HLGSMHERLPGETRLGNSGITFAPDGGIAARYRKVHLYDAVVNGKPYRESADFAPGDGLHTVDAAGLTLGLSICYDLRFG
ELYRTLRARGANVLLVPAAFNVHTGRDHWETLLRARAIENQCYVIAAAQIGGPGPALPCLGRSMIIDPWGTVLACMPDTT
GFITAELDTERVAGLREALPAWEHRRTDLYFGGIQNSG

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=271, Percent_Identity=34.3173431734317, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI297632348, Length=272, Percent_Identity=38.9705882352941, Blast_Score=174, Evalue=6e-44,
Organism=Homo sapiens, GI297632350, Length=272, Percent_Identity=38.9705882352941, Blast_Score=174, Evalue=6e-44,
Organism=Homo sapiens, GI5031947, Length=272, Percent_Identity=38.9705882352941, Blast_Score=174, Evalue=8e-44,
Organism=Homo sapiens, GI297632346, Length=194, Percent_Identity=34.5360824742268, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1786412, Length=233, Percent_Identity=30.0429184549356, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556280, Length=269, Percent_Identity=39.4052044609665, Blast_Score=185, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6323383, Length=276, Percent_Identity=32.2463768115942, Blast_Score=151, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6322335, Length=301, Percent_Identity=33.2225913621262, Blast_Score=146, Evalue=4e-36,
Organism=Drosophila melanogaster, GI17933642, Length=268, Percent_Identity=39.1791044776119, Blast_Score=181, Evalue=5e-46,
Organism=Drosophila melanogaster, GI21355835, Length=270, Percent_Identity=30, Blast_Score=145, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 29908; Mature: 29908

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRIKVAAIQIDSRQDREANLAALEHWILAAASDGAKLIVTPEYSDVRGDANALQAAASA
CEEEEEEEEEECCCCCCCHHHHHHHHHHEEECCCCCEEEEECCCCCCCCCHHHHHHHHHC
VPGPVSEHISSLAQRTGCWIHLGSMHERLPGETRLGNSGITFAPDGGIAARYRKVHLYDA
CCCCHHHHHHHHHHHCCCEEEECCHHHHCCCCCCCCCCCCEECCCCCCCHHEEEEEEEEE
VVNGKPYRESADFAPGDGLHTVDAAGLTLGLSICYDLRFGELYRTLRARGANVLLVPAAF
EECCCCCCCCCCCCCCCCCCEECHHHHHEEEEHEEECHHHHHHHHHHHCCCCEEEEEEEE
NVHTGRDHWETLLRARAIENQCYVIAAAQIGGPGPALPCLGRSMIIDPWGTVLACMPDTT
EECCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHCCCEEEECCCCCEEEECCCCC
GFITAELDTERVAGLREALPAWEHRRTDLYFGGIQNSG
CEEEEECCHHHHHHHHHHCCHHHCCCCEEEEECCCCCC
>Mature Secondary Structure
MMRIKVAAIQIDSRQDREANLAALEHWILAAASDGAKLIVTPEYSDVRGDANALQAAASA
CEEEEEEEEEECCCCCCCHHHHHHHHHHEEECCCCCEEEEECCCCCCCCCHHHHHHHHHC
VPGPVSEHISSLAQRTGCWIHLGSMHERLPGETRLGNSGITFAPDGGIAARYRKVHLYDA
CCCCHHHHHHHHHHHCCCEEEECCHHHHCCCCCCCCCCCCEECCCCCCCHHEEEEEEEEE
VVNGKPYRESADFAPGDGLHTVDAAGLTLGLSICYDLRFGELYRTLRARGANVLLVPAAF
EECCCCCCCCCCCCCCCCCCEECHHHHHEEEEHEEECHHHHHHHHHHHCCCCEEEEEEEE
NVHTGRDHWETLLRARAIENQCYVIAAAQIGGPGPALPCLGRSMIIDPWGTVLACMPDTT
EECCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHCCCEEEECCCCCEEEECCCCC
GFITAELDTERVAGLREALPAWEHRRTDLYFGGIQNSG
CEEEEECCHHHHHHHHHHCCHHHCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]