Click here to switch to the map view.

The map label for this gene is yflS [H]

Identifier: 94313229

GI number: 94313229

Start: 897790

End: 899295

Strand: Direct

Name: yflS [H]

Synonym: Rmet_4303

Alternate gene names: 94313229

Gene position: 897790-899295 (Clockwise)

Preceding gene: 94313224

Following gene: 94313236

Centisome position: 34.8

GC content: 59.56

Gene sequence:

>1506_bases
ATGTTCGCCAAGATCGTCGCGACATATCGCTACTTCATGTCCGTGGTGCCTTTTCGGCTGACGCCGGCCATCATCACCAC
GGTTGTGCTTGTCACGCTGCTGCTCACACCGACGCCGGCTGGTTTGAAGGACAACGCTTGGGATCTGGTCGCCATCTTTC
TGACCACGATCGTCGCCATCATTCTCAAGGTGATGCCGATCGGTGTGATGGCGATGATGGCGATCGTGATCGTGTCACTG
GCGCAAGTGACCTCGAACTCGTCCAAGGGCGCCATTGCCGATGCGCTGAGCAGTTTTGATAGTCCGCTGATCTGGCTGAT
CGTTGTGGCCATCCTGATTTCGCGCGGCCTGAAGAAGACGGGGTTGGGCAGTCGCATCGGCCTGATGTTCATCGCGCTAT
TGGGCAAGCGGACGGTCGGCATTGGCTACGGGCTGGCGATCTGCGAGCTGGTACTGGCGCCATTCACGCCGAGCAACACC
GCGCGCGGCGGCGGCATTGTGCACCCGATCATGCGGTCGATTGCCGGCGCCTTCGATTCCGATCCGGCTAACGGGACTCA
GGGAAAGGTTGGTACCTATCTGGCACTAGTCAACTACCACGCCAACCCGATCACTTCGGCCATGTTCGTGACCGCCACCG
CGCCGAACCCCCTGGTCGTCGACTACGTGGCCAAGGCCAGTGGCCAGTCGCTTCACCTGAGCTGGACCACCTGGGCGCTG
TGCATGCTGCTGCCAGGCCTCGTCTGCCTGCTTCTGATGCCGCTTGTCATTCGCGTGCTCTGTCCGCCCGAACTGAAGGC
GACCCCCAATGCGATCGAATACGCCAAGGGTGAGCTTGCTCGCATGGGCCCCCTGGGTGGCAAAGAGCGCGTCATGATTG
GTGTCTTCGCCATGATGCTGGTTCTGTGGGCCAACGTGCCGGCAATGATCTGGGGGCCGACGTTCACGCTCGACCCGACG
GTGGTCGCCTTTCTCGGCCTGTTCGCGTTGATCATTACCGGCACGATCGACTGGGACGATGTGTTATCGGAGAAGAGCGC
CTGGGACACCCTGATCTGGTTCGGCGCTCTGGTAATGCTCGCGGAACAGCTCAACAAGCTTGGCGTAATCGCATGGTTCT
CGGCCGATATGCGCGATGCCATTGCCGCCAGTGGAATGGGTTGGCTCTCGATTGCCGCCATATTGGTGCTGGCGTTCGTG
TTTTCCCATTACCTCTTCGCAAGTACCACTGCCCATATCAGCGCGATGATGCTGGCCTTTCTGACAGTGGGCGCGCAGTT
GATTCCGCAGCCTTATATCGCACCTTTCATGCTGATGATGACCGCCGGCTCGGCGATCATGATGACGCTGACCCACTATG
CCACCGGCACATCGCCGATCATATTTGGCAGCGGGTATGTGACGATGGGGCAATGGTGGCGGGTCGGCGCGGTGATGTGT
GTCTTTGAACTTGTCATCTTCGCCGTAGTAGGAGGTATCTGGTGGAAGATTCTGGGGTTCTGGTAG

Upstream 100 bases:

>100_bases
GGCAAGTGCGCTGCACCGCCGGGAGGAACGGTTCGTGTCGGCAGACGCAGAGACATTGGCTGTCTTCATTTCGCCGTGTC
CTTTTCGAGGAGGTTCGGAG

Downstream 100 bases:

>100_bases
TCCGGGGCCACGGCTACTCAAAGGGGCTTTTGTCGACCGGGACTCGACGGTGTTTGATCGGCTGATTAGCACCGCCTATA
CGCAGCACAATCTCTTTATC

Product: anion transporter

Products: tartrate [Cytoplasm]; succinate [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MFAKIVATYRYFMSVVPFRLTPAIITTVVLVTLLLTPTPAGLKDNAWDLVAIFLTTIVAIILKVMPIGVMAMMAIVIVSL
AQVTSNSSKGAIADALSSFDSPLIWLIVVAILISRGLKKTGLGSRIGLMFIALLGKRTVGIGYGLAICELVLAPFTPSNT
ARGGGIVHPIMRSIAGAFDSDPANGTQGKVGTYLALVNYHANPITSAMFVTATAPNPLVVDYVAKASGQSLHLSWTTWAL
CMLLPGLVCLLLMPLVIRVLCPPELKATPNAIEYAKGELARMGPLGGKERVMIGVFAMMLVLWANVPAMIWGPTFTLDPT
VVAFLGLFALIITGTIDWDDVLSEKSAWDTLIWFGALVMLAEQLNKLGVIAWFSADMRDAIAASGMGWLSIAAILVLAFV
FSHYLFASTTAHISAMMLAFLTVGAQLIPQPYIAPFMLMMTAGSAIMMTLTHYATGTSPIIFGSGYVTMGQWWRVGAVMC
VFELVIFAVVGGIWWKILGFW

Sequences:

>Translated_501_residues
MFAKIVATYRYFMSVVPFRLTPAIITTVVLVTLLLTPTPAGLKDNAWDLVAIFLTTIVAIILKVMPIGVMAMMAIVIVSL
AQVTSNSSKGAIADALSSFDSPLIWLIVVAILISRGLKKTGLGSRIGLMFIALLGKRTVGIGYGLAICELVLAPFTPSNT
ARGGGIVHPIMRSIAGAFDSDPANGTQGKVGTYLALVNYHANPITSAMFVTATAPNPLVVDYVAKASGQSLHLSWTTWAL
CMLLPGLVCLLLMPLVIRVLCPPELKATPNAIEYAKGELARMGPLGGKERVMIGVFAMMLVLWANVPAMIWGPTFTLDPT
VVAFLGLFALIITGTIDWDDVLSEKSAWDTLIWFGALVMLAEQLNKLGVIAWFSADMRDAIAASGMGWLSIAAILVLAFV
FSHYLFASTTAHISAMMLAFLTVGAQLIPQPYIAPFMLMMTAGSAIMMTLTHYATGTSPIIFGSGYVTMGQWWRVGAVMC
VFELVIFAVVGGIWWKILGFW
>Mature_501_residues
MFAKIVATYRYFMSVVPFRLTPAIITTVVLVTLLLTPTPAGLKDNAWDLVAIFLTTIVAIILKVMPIGVMAMMAIVIVSL
AQVTSNSSKGAIADALSSFDSPLIWLIVVAILISRGLKKTGLGSRIGLMFIALLGKRTVGIGYGLAICELVLAPFTPSNT
ARGGGIVHPIMRSIAGAFDSDPANGTQGKVGTYLALVNYHANPITSAMFVTATAPNPLVVDYVAKASGQSLHLSWTTWAL
CMLLPGLVCLLLMPLVIRVLCPPELKATPNAIEYAKGELARMGPLGGKERVMIGVFAMMLVLWANVPAMIWGPTFTLDPT
VVAFLGLFALIITGTIDWDDVLSEKSAWDTLIWFGALVMLAEQLNKLGVIAWFSADMRDAIAASGMGWLSIAAILVLAFV
FSHYLFASTTAHISAMMLAFLTVGAQLIPQPYIAPFMLMMTAGSAIMMTLTHYATGTSPIIFGSGYVTMGQWWRVGAVMC
VFELVIFAVVGGIWWKILGFW

Specific function: Might be a malate transporter [H]

COG id: COG0471

COG function: function code P; Di- and tricarboxylate transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SLC13A transporter (TC 2.A.47) family [H]

Homologues:

Organism=Escherichia coli, GI1789444, Length=479, Percent_Identity=36.1169102296451, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI1786829, Length=486, Percent_Identity=33.9506172839506, Blast_Score=265, Evalue=7e-72,
Organism=Escherichia coli, GI1786986, Length=470, Percent_Identity=35.531914893617, Blast_Score=263, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001898 [H]

Pfam domain/function: PF00939 Na_sulph_symp [H]

EC number: NA

Molecular weight: Translated: 53736; Mature: 53736

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAKIVATYRYFMSVVPFRLTPAIITTVVLVTLLLTPTPAGLKDNAWDLVAIFLTTIVAI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
ILKVMPIGVMAMMAIVIVSLAQVTSNSSKGAIADALSSFDSPLIWLIVVAILISRGLKKT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
GLGSRIGLMFIALLGKRTVGIGYGLAICELVLAPFTPSNTARGGGIVHPIMRSIAGAFDS
CCCHHHHHHHHHHHCCCHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCC
DPANGTQGKVGTYLALVNYHANPITSAMFVTATAPNPLVVDYVAKASGQSLHLSWTTWAL
CCCCCCCCCHHHEEEEEECCCCCCCCEEEEEECCCCCEEHHHHHHCCCCEEEEHHHHHHH
CMLLPGLVCLLLMPLVIRVLCPPELKATPNAIEYAKGELARMGPLGGKERVMIGVFAMML
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHCCCCCCCHHHHHHHHHHHH
VLWANVPAMIWGPTFTLDPTVVAFLGLFALIITGTIDWDDVLSEKSAWDTLIWFGALVML
HHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
AEQLNKLGVIAWFSADMRDAIAASGMGWLSIAAILVLAFVFSHYLFASTTAHISAMMLAF
HHHHHHCCEEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTVGAQLIPQPYIAPFMLMMTAGSAIMMTLTHYATGTSPIIFGSGYVTMGQWWRVGAVMC
HHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEECCCCEEHHHHHHHHHHHH
VFELVIFAVVGGIWWKILGFW
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFAKIVATYRYFMSVVPFRLTPAIITTVVLVTLLLTPTPAGLKDNAWDLVAIFLTTIVAI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
ILKVMPIGVMAMMAIVIVSLAQVTSNSSKGAIADALSSFDSPLIWLIVVAILISRGLKKT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
GLGSRIGLMFIALLGKRTVGIGYGLAICELVLAPFTPSNTARGGGIVHPIMRSIAGAFDS
CCCHHHHHHHHHHHCCCHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCC
DPANGTQGKVGTYLALVNYHANPITSAMFVTATAPNPLVVDYVAKASGQSLHLSWTTWAL
CCCCCCCCCHHHEEEEEECCCCCCCCEEEEEECCCCCEEHHHHHHCCCCEEEEHHHHHHH
CMLLPGLVCLLLMPLVIRVLCPPELKATPNAIEYAKGELARMGPLGGKERVMIGVFAMML
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHCCCCCCCHHHHHHHHHHHH
VLWANVPAMIWGPTFTLDPTVVAFLGLFALIITGTIDWDDVLSEKSAWDTLIWFGALVML
HHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
AEQLNKLGVIAWFSADMRDAIAASGMGWLSIAAILVLAFVFSHYLFASTTAHISAMMLAF
HHHHHHCCEEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTVGAQLIPQPYIAPFMLMMTAGSAIMMTLTHYATGTSPIIFGSGYVTMGQWWRVGAVMC
HHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEECCCCEEHHHHHHHHHHHH
VFELVIFAVVGGIWWKILGFW
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: tartrate [Periplasm]; succinate [Cytoplasm] [C]

Specific reaction: tartrate [Periplasm] + succinate [Cytoplasm] = tartrate [Cytoplasm] + succinate [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 12949159 [H]