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The map label for this gene is prpB [H]

Identifier: 94313217

GI number: 94313217

Start: 882415

End: 883302

Strand: Reverse

Name: prpB [H]

Synonym: Rmet_4291

Alternate gene names: 94313217

Gene position: 883302-882415 (Counterclockwise)

Preceding gene: 94313219

Following gene: 94313216

Centisome position: 34.24

GC content: 60.47

Gene sequence:

>888_bases
ATGACTACCTTATCCGCCGCCCGCCGTGCGGCATTCCGCGCCAAGGTCAATGAACGCCGAGGGCTGCTGGTGGCTGGCGC
CTTCAATGCAATGAGCGCCCGCGTCGTCGAGGAAACCGGGTTCGAGGCGCTATACCTGACGGGAGCCGGCGTTACGAATA
TGTCGCTTGGCCTACCCGATCTCGGTTTCATCGGCCTGCATGAAATTGCCGAACACACCGCGCGGGTCCGCGATGCGGTC
GCGCTACCATTGATCGTCGATGCCGACACCGGTTTCGGCAATGCGCTCAACGTACGGCACACGGTAAGAACGCTCGAACG
CAGCGGCGCCGATGCGATCCAGATTGAAGATCAGGTGATGCCCAAGAAATGCGGACATTTCTCGGGCAAGGAGGTCATCG
CGACGAGTGAAATGCTTGGCAAGATCCGCGCTGCCGTTGATGCGCGCGAGGACCCCAACCTGCTGATCATGGCGCGCACG
GATGCCGCAGCGGTGCATGGCATGGAGGCTGCCATCGAGCGCGGCCATCGCTTCATTGAGGCGGGGGCGGACATTCTCTT
CATCGAAGCGACCGAGTCACTTGCCGACGTCGAGCGTTTGCCCAAGCTGATCGCTGCGCCACAACTGATCAATATTGTGA
TCGGCGGGAAGACGCCAGTACAGTCGCGCGAAACGCTAGCCAGCCATGGCTACGCACTTGTGCTGTATGCGAATGCAACG
CTACAAGGTGCAGTGCTTGGTATGCAGAGGGCGCTCAGCACGCTGCGCACGAATGGTCGGCTTGACGAGGATGCGACGCT
GGTGGCGCCATTCAGCGAGCGGCAACGCTTGGTCAATAAGCCTCTATACGACCGGCTCGATCGCGAATACGCAGCAAACG
ATCATTGA

Upstream 100 bases:

>100_bases
AAATCTCGTAAGCCATTGTTTTTGAAAGAAACAACGGCAGGTGCGGGAACTTGGCACGATCCGTGCGAAAACACCGTCTC
CTACTCTGGAGACACATCAA

Downstream 100 bases:

>100_bases
ACGGGAGGGCCACGCCGGAAGAGTTTCCAAGAGGATCGTGGCTCCCGGACAACTGAACAGGTAGATTTCACCGCAGCGCA
CAGCACGTTGGGGTCAAGGT

Product: 2,3-dimethylmalate lyase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MTTLSAARRAAFRAKVNERRGLLVAGAFNAMSARVVEETGFEALYLTGAGVTNMSLGLPDLGFIGLHEIAEHTARVRDAV
ALPLIVDADTGFGNALNVRHTVRTLERSGADAIQIEDQVMPKKCGHFSGKEVIATSEMLGKIRAAVDAREDPNLLIMART
DAAAVHGMEAAIERGHRFIEAGADILFIEATESLADVERLPKLIAAPQLINIVIGGKTPVQSRETLASHGYALVLYANAT
LQGAVLGMQRALSTLRTNGRLDEDATLVAPFSERQRLVNKPLYDRLDREYAANDH

Sequences:

>Translated_295_residues
MTTLSAARRAAFRAKVNERRGLLVAGAFNAMSARVVEETGFEALYLTGAGVTNMSLGLPDLGFIGLHEIAEHTARVRDAV
ALPLIVDADTGFGNALNVRHTVRTLERSGADAIQIEDQVMPKKCGHFSGKEVIATSEMLGKIRAAVDAREDPNLLIMART
DAAAVHGMEAAIERGHRFIEAGADILFIEATESLADVERLPKLIAAPQLINIVIGGKTPVQSRETLASHGYALVLYANAT
LQGAVLGMQRALSTLRTNGRLDEDATLVAPFSERQRLVNKPLYDRLDREYAANDH
>Mature_294_residues
TTLSAARRAAFRAKVNERRGLLVAGAFNAMSARVVEETGFEALYLTGAGVTNMSLGLPDLGFIGLHEIAEHTARVRDAVA
LPLIVDADTGFGNALNVRHTVRTLERSGADAIQIEDQVMPKKCGHFSGKEVIATSEMLGKIRAAVDAREDPNLLIMARTD
AAAVHGMEAAIERGHRFIEAGADILFIEATESLADVERLPKLIAAPQLINIVIGGKTPVQSRETLASHGYALVLYANATL
QGAVLGMQRALSTLRTNGRLDEDATLVAPFSERQRLVNKPLYDRLDREYAANDH

Specific function: Catalyzes the formation of proponate and pyruvate from (2R,3S)-2,3-dimethylmalate. Has no activity toward dimethylmaleate, malate, citramalate, isocitrate and citrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=250, Percent_Identity=42, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1790445, Length=87, Percent_Identity=42.5287356321839, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.32 [H]

Molecular weight: Translated: 31833; Mature: 31702

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLSAARRAAFRAKVNERRGLLVAGAFNAMSARVVEETGFEALYLTGAGVTNMSLGLPD
CCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
LGFIGLHEIAEHTARVRDAVALPLIVDADTGFGNALNVRHTVRTLERSGADAIQIEDQVM
CCHHHHHHHHHHHHHHHHHEECEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECCHHC
PKKCGHFSGKEVIATSEMLGKIRAAVDAREDPNLLIMARTDAAAVHGMEAAIERGHRFIE
HHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHCHHEEC
AGADILFIEATESLADVERLPKLIAAPQLINIVIGGKTPVQSRETLASHGYALVLYANAT
CCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCEEEEEEECCC
LQGAVLGMQRALSTLRTNGRLDEDATLVAPFSERQRLVNKPLYDRLDREYAANDH
HHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TTLSAARRAAFRAKVNERRGLLVAGAFNAMSARVVEETGFEALYLTGAGVTNMSLGLPD
CCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
LGFIGLHEIAEHTARVRDAVALPLIVDADTGFGNALNVRHTVRTLERSGADAIQIEDQVM
CCHHHHHHHHHHHHHHHHHEECEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECCHHC
PKKCGHFSGKEVIATSEMLGKIRAAVDAREDPNLLIMARTDAAAVHGMEAAIERGHRFIE
HHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHCHHEEC
AGADILFIEATESLADVERLPKLIAAPQLINIVIGGKTPVQSRETLASHGYALVLYANAT
CCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCEEEEEEECCC
LQGAVLGMQRALSTLRTNGRLDEDATLVAPFSERQRLVNKPLYDRLDREYAANDH
HHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA