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The map label for this gene is nac [H]

Identifier: 94313133

GI number: 94313133

Start: 790294

End: 791244

Strand: Direct

Name: nac [H]

Synonym: Rmet_4206

Alternate gene names: 94313133

Gene position: 790294-791244 (Clockwise)

Preceding gene: 94313132

Following gene: 94313134

Centisome position: 30.63

GC content: 65.3

Gene sequence:

>951_bases
ATGGAATTGCGCCAGCTTCAATACTTCGTGCGGGTGGTCGAACTCGGCAGCATGAGCCGGGCGGCGCTTGAGCTCGACAT
GGTGCAATCGGCCGTCAGCCAGCAGATCAGCCGGCTCGAGAGCGAACTGGCCACGCGCCTGCTGCGCCGGACGCCACACG
GCGTGACGCCGACCGAAGCGGGCCTTGCGTTCTTTCACGAAGCCCAGCTTACTCTGCGTCACGCGGAGCAGGCGCGGCGC
GCTGCGCATCAGGCGCGGCTGACCGGTACGGTCAGTGTCGGGCTGGCGCCGACCACGGCCTCGGTGATCGGTGTGCCGCT
GATTCGCGCGATGCGCGAGCGCTATCCCGACGTGCGACTGCATATCGTTGAGAGCCTGTCGGGACACCTGACCGCGATGC
TCAATGCGCGCCAGCTCGATCTGGCCGTGTTGTTCGATACGCATGCCGCGCGGCGCTGGACGGTCATGCCGCTGCTCGAG
GAAACGCTCTTCCTGATTCAGTCCCGCAATGCCTTGCCGCAGGGCAAGGCCGAGCCGCCCACGCGTCTGACCATGGCCGA
TCTGTCCGAGGTGCCGCTGATCATGCCCACCGGTGTTCATGGTCTGCGCAGCACGCTCGATGCGGCATTCTCCCGGGCCA
AGGTTGTACCGCATGTCACGCAGGAGATCGATTCGCTGGCGATGCTGATGGATGCCGTCGACATGGGCTTTGGCACCACG
CTGCAGCCATGGGCCGCAGTGGGGCGCTATCCCGATGCGGAAACGCGTTTTCACCTGGCGCATATCGACGATCCGCAGGT
AGGTCGCCAGAACCTGCTTTGCAGTCTGTCGGACGATGAGTTGTCACCCGCTGCGCTGGCGCTGCGGGTGGTGCTGGCCG
ATACGGCGCGTGGTCTGGTGCGATCGGGAGGTTGGCGCGGGGCACGGCTGCTAGAGGCACTATCAAAATGA

Upstream 100 bases:

>100_bases
CGGCATCTCGGTCCGCCGCGTTTCGCATCTGGTATAAGACGTCCCACGAGATGACGATCGTTGCCGCCGGAATCGGCGGT
GGCGATGCCAGGGGACACAG

Downstream 100 bases:

>100_bases
TTCTGGCGTCGTTGCGCGGCCTTGGCCGGTCCACTTGTACTGTCTGCGGCCGCCCCCTTGCAGGGCGCGCATGTGCTCGC
AGGCACATGCCGTTCCGCAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MELRQLQYFVRVVELGSMSRAALELDMVQSAVSQQISRLESELATRLLRRTPHGVTPTEAGLAFFHEAQLTLRHAEQARR
AAHQARLTGTVSVGLAPTTASVIGVPLIRAMRERYPDVRLHIVESLSGHLTAMLNARQLDLAVLFDTHAARRWTVMPLLE
ETLFLIQSRNALPQGKAEPPTRLTMADLSEVPLIMPTGVHGLRSTLDAAFSRAKVVPHVTQEIDSLAMLMDAVDMGFGTT
LQPWAAVGRYPDAETRFHLAHIDDPQVGRQNLLCSLSDDELSPAALALRVVLADTARGLVRSGGWRGARLLEALSK

Sequences:

>Translated_316_residues
MELRQLQYFVRVVELGSMSRAALELDMVQSAVSQQISRLESELATRLLRRTPHGVTPTEAGLAFFHEAQLTLRHAEQARR
AAHQARLTGTVSVGLAPTTASVIGVPLIRAMRERYPDVRLHIVESLSGHLTAMLNARQLDLAVLFDTHAARRWTVMPLLE
ETLFLIQSRNALPQGKAEPPTRLTMADLSEVPLIMPTGVHGLRSTLDAAFSRAKVVPHVTQEIDSLAMLMDAVDMGFGTT
LQPWAAVGRYPDAETRFHLAHIDDPQVGRQNLLCSLSDDELSPAALALRVVLADTARGLVRSGGWRGARLLEALSK
>Mature_316_residues
MELRQLQYFVRVVELGSMSRAALELDMVQSAVSQQISRLESELATRLLRRTPHGVTPTEAGLAFFHEAQLTLRHAEQARR
AAHQARLTGTVSVGLAPTTASVIGVPLIRAMRERYPDVRLHIVESLSGHLTAMLNARQLDLAVLFDTHAARRWTVMPLLE
ETLFLIQSRNALPQGKAEPPTRLTMADLSEVPLIMPTGVHGLRSTLDAAFSRAKVVPHVTQEIDSLAMLMDAVDMGFGTT
LQPWAAVGRYPDAETRFHLAHIDDPQVGRQNLLCSLSDDELSPAALALRVVLADTARGLVRSGGWRGARLLEALSK

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=249, Percent_Identity=35.7429718875502, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI157672245, Length=244, Percent_Identity=28.6885245901639, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1790399, Length=249, Percent_Identity=28.5140562248996, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787879, Length=311, Percent_Identity=29.2604501607717, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788748, Length=300, Percent_Identity=27.3333333333333, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787601, Length=180, Percent_Identity=31.1111111111111, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788887, Length=288, Percent_Identity=26.3888888888889, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786401, Length=189, Percent_Identity=32.8042328042328, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789639, Length=213, Percent_Identity=28.169014084507, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787806, Length=248, Percent_Identity=26.2096774193548, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787530, Length=243, Percent_Identity=25.1028806584362, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34705; Mature: 34705

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRQLQYFVRVVELGSMSRAALELDMVQSAVSQQISRLESELATRLLRRTPHGVTPTEA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
GLAFFHEAQLTLRHAEQARRAAHQARLTGTVSVGLAPTTASVIGVPLIRAMRERYPDVRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHH
HIVESLSGHLTAMLNARQLDLAVLFDTHAARRWTVMPLLEETLFLIQSRNALPQGKAEPP
HHHHHHHHHHHHHHCHHHHEEEEEEECCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCC
TRLTMADLSEVPLIMPTGVHGLRSTLDAAFSRAKVVPHVTQEIDSLAMLMDAVDMGFGTT
CEEEHHHHCCCCEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCC
LQPWAAVGRYPDAETRFHLAHIDDPQVGRQNLLCSLSDDELSPAALALRVVLADTARGLV
CCHHHHHCCCCCCCCEEEEEECCCCCCCCHHEEEECCCCCCCHHHHHHHHHHHHHHHHHH
RSGGWRGARLLEALSK
HCCCCCHHHHHHHHCC
>Mature Secondary Structure
MELRQLQYFVRVVELGSMSRAALELDMVQSAVSQQISRLESELATRLLRRTPHGVTPTEA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
GLAFFHEAQLTLRHAEQARRAAHQARLTGTVSVGLAPTTASVIGVPLIRAMRERYPDVRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHH
HIVESLSGHLTAMLNARQLDLAVLFDTHAARRWTVMPLLEETLFLIQSRNALPQGKAEPP
HHHHHHHHHHHHHHCHHHHEEEEEEECCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCC
TRLTMADLSEVPLIMPTGVHGLRSTLDAAFSRAKVVPHVTQEIDSLAMLMDAVDMGFGTT
CEEEHHHHCCCCEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCC
LQPWAAVGRYPDAETRFHLAHIDDPQVGRQNLLCSLSDDELSPAALALRVVLADTARGLV
CCHHHHHCCCCCCCCEEEEEECCCCCCCCHHEEEECCCCCCCHHHHHHHHHHHHHHHHHH
RSGGWRGARLLEALSK
HCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8458853 [H]