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The map label for this gene is cheD [H]

Identifier: 94313122

GI number: 94313122

Start: 779982

End: 781529

Strand: Direct

Name: cheD [H]

Synonym: Rmet_4195

Alternate gene names: 94313122

Gene position: 779982-781529 (Clockwise)

Preceding gene: 94313120

Following gene: 94313125

Centisome position: 30.23

GC content: 64.28

Gene sequence:

>1548_bases
ATGAATAACCTGAGCATCCGCCACGGCCTGCTGGCGACCCTGGTGATTTTCGGCCTGATGATCGTGTTTGGCGCCGCGCT
GGGCGTGACCCAGTTGTACGCAGCCAGCGAGTCCGCGGAGGACATGCATCAGGTATCGGCGCACGTGCAGTTGCTTAACA
GCGCCTACAAGGACATGACACGCGCGCGCGCTGCGCTGACGCGTGCGTACAACTCGGCCAAAGCTGGCGGCGGCATCAAC
GCCGAGGCAATCGGCAATGCGGAGGGCTCGATCCGCAAGTCCACCGATGCGTTGGAACGCTTTGCCAGCGCGCCTGTCCT
GGAGGGCCTGGATGCCTCGGCACGCGACGGCATGGTTGCTGTCGCACGGGCACACAATGACGCTGTGCAACGGGGACTCG
AAGCATTGCGTAAGGATGATCCCGATGGCTTCGTGGCGATCAACGACAAGGACATCACCGCGACAGGTATGAAGTATTCA
GCTGATGTCGAGCGTTTCGAGGCGCTCGCCACCCAGCACACTGAAGCCGTGATCGCCAGGATCAGCACCCGTTTCAACCG
GGTGCTGATCCTGGTGTGTGTCGGCATGGTGGCGTCGGTGCTGCTGATCGTGGTCGTGCATCTGGCGCTGCGCAAGCTGG
TTGTGGCGCCGCTGCACCAGGCGTGCGACCTGATCATGCGCGTGGCGGACGGCGATCTGACGATCAAGGTGCCCGAAGCC
GGCCGCAATGAAATCGGACAGTTGCTGCGCGCGCTGTCGCGAATGCAGCATGGGCTGACGGACACCGTGGCGAAGGTGCG
GGCGGGCTCCGATGCGGTGACAACAGGCGCGCGGGAGATTGCCGCGGGCAATACCGACCTGTCGTCGCGTACCGAGCAGC
AATCGTCGGCGCTGGAGCAGACGGCCGCCAGCATGGAGGAGTTGACGTCGACGGTGGCCAATAACGCGGAGAGCGCCACG
CGCGCCAGCGGCCTGGCCCGCGATGCGGCGGATCTGGCATCGCGCGGTGGCGAGGTGGTGCGTGGCGTGGTGCAGACGAT
GAGCGAGATCAACGCCAGCTCGCAGAAGATCGTGGACATCATCGCCGTAATCGACGGCATTGCATTCCAGACCAACATCC
TTGCGCTCAATGCCGCCGTGGAAGCGGCACGTGCTGGCGAGCAGGGTCGCGGTTTCGCGGTGGTGGCTGGCGAGGTGCGC
GCGCTGGCTCAGCGCAGCGCAGGAGCCGCCAAGGAGATCAAGTCGTTGATCGATTCGTCCGTCGACAAGGTAGGCGCCGG
CAGCGCGCAGGTAGAGCAGGCCGGCAGCACGATCGAGGACATCGTGGTGGCCGTGCGGCGACTGGCCGATACCGTCAACG
AGATCTCGGCTGCATCGATCGAACAGTCTTCAGGTATCTCGCAGGTCAGCGAGGCGGTGTCGCAGATGGAGCAGGTGAAC
CAGCAGAACGCTGCATTGGTGGAGCAGGCTGCTGCTGCCGCAGATTCGCTCGAATCCCATGCCAACCAGCTTGCCGCCGC
CGTGACAACGTTCAAGCTGGCGGCTTGA

Upstream 100 bases:

>100_bases
GCCTCGCATTTGATCTATCGCAAATGAAGATCATGGGTAGGCTCAAGCTTCAGACGAGGGCGTCGTTAAGGCGGGCTTGC
ACCCTTATCTGTTGAGCCCC

Downstream 100 bases:

>100_bases
TGGTTCGTCTGAAGGCCCGCCCGAGGGGGATTTACCCGGGCGGGTGGCATTAGCCGGCGAGCTGTTCCTCGACGAACGTC
AGCCGGTCGTTGCCCCAGAA

Product: methyl-accepting chemotaxis sensory transducer

Products: NA

Alternate protein names: Citrate chemoreceptor protein [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MNNLSIRHGLLATLVIFGLMIVFGAALGVTQLYAASESAEDMHQVSAHVQLLNSAYKDMTRARAALTRAYNSAKAGGGIN
AEAIGNAEGSIRKSTDALERFASAPVLEGLDASARDGMVAVARAHNDAVQRGLEALRKDDPDGFVAINDKDITATGMKYS
ADVERFEALATQHTEAVIARISTRFNRVLILVCVGMVASVLLIVVVHLALRKLVVAPLHQACDLIMRVADGDLTIKVPEA
GRNEIGQLLRALSRMQHGLTDTVAKVRAGSDAVTTGAREIAAGNTDLSSRTEQQSSALEQTAASMEELTSTVANNAESAT
RASGLARDAADLASRGGEVVRGVVQTMSEINASSQKIVDIIAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVR
ALAQRSAGAAKEIKSLIDSSVDKVGAGSAQVEQAGSTIEDIVVAVRRLADTVNEISAASIEQSSGISQVSEAVSQMEQVN
QQNAALVEQAAAAADSLESHANQLAAAVTTFKLAA

Sequences:

>Translated_515_residues
MNNLSIRHGLLATLVIFGLMIVFGAALGVTQLYAASESAEDMHQVSAHVQLLNSAYKDMTRARAALTRAYNSAKAGGGIN
AEAIGNAEGSIRKSTDALERFASAPVLEGLDASARDGMVAVARAHNDAVQRGLEALRKDDPDGFVAINDKDITATGMKYS
ADVERFEALATQHTEAVIARISTRFNRVLILVCVGMVASVLLIVVVHLALRKLVVAPLHQACDLIMRVADGDLTIKVPEA
GRNEIGQLLRALSRMQHGLTDTVAKVRAGSDAVTTGAREIAAGNTDLSSRTEQQSSALEQTAASMEELTSTVANNAESAT
RASGLARDAADLASRGGEVVRGVVQTMSEINASSQKIVDIIAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVR
ALAQRSAGAAKEIKSLIDSSVDKVGAGSAQVEQAGSTIEDIVVAVRRLADTVNEISAASIEQSSGISQVSEAVSQMEQVN
QQNAALVEQAAAAADSLESHANQLAAAVTTFKLAA
>Mature_515_residues
MNNLSIRHGLLATLVIFGLMIVFGAALGVTQLYAASESAEDMHQVSAHVQLLNSAYKDMTRARAALTRAYNSAKAGGGIN
AEAIGNAEGSIRKSTDALERFASAPVLEGLDASARDGMVAVARAHNDAVQRGLEALRKDDPDGFVAINDKDITATGMKYS
ADVERFEALATQHTEAVIARISTRFNRVLILVCVGMVASVLLIVVVHLALRKLVVAPLHQACDLIMRVADGDLTIKVPEA
GRNEIGQLLRALSRMQHGLTDTVAKVRAGSDAVTTGAREIAAGNTDLSSRTEQQSSALEQTAASMEELTSTVANNAESAT
RASGLARDAADLASRGGEVVRGVVQTMSEINASSQKIVDIIAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVR
ALAQRSAGAAKEIKSLIDSSVDKVGAGSAQVEQAGSTIEDIVVAVRRLADTVNEISAASIEQSSGISQVSEAVSQMEQVN
QQNAALVEQAAAAADSLESHANQLAAAVTTFKLAA

Specific function: Acts as a receptor for citrate and mediates taxis away from phenol. Also mediates an attractant response to metal-citrate complexes [H]

COG id: COG0840

COG function: function code NT; Methyl-accepting chemotaxis protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1788195, Length=306, Percent_Identity=61.7647058823529, Blast_Score=328, Evalue=7e-91,
Organism=Escherichia coli, GI1787690, Length=337, Percent_Identity=54.3026706231454, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI2367378, Length=536, Percent_Identity=43.4701492537313, Blast_Score=318, Evalue=6e-88,
Organism=Escherichia coli, GI1788194, Length=529, Percent_Identity=40.8317580340265, Blast_Score=305, Evalue=5e-84,
Organism=Escherichia coli, GI1789453, Length=328, Percent_Identity=39.9390243902439, Blast_Score=212, Evalue=4e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR003122
- InterPro:   IPR004091
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00672 HAMP; PF00015 MCPsignal; PF02203 TarH [H]

EC number: NA

Molecular weight: Translated: 53568; Mature: 53568

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLSIRHGLLATLVIFGLMIVFGAALGVTQLYAASESAEDMHQVSAHVQLLNSAYKDMT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RARAALTRAYNSAKAGGGINAEAIGNAEGSIRKSTDALERFASAPVLEGLDASARDGMVA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHCCCCCCCCCCEEE
VARAHNDAVQRGLEALRKDDPDGFVAINDKDITATGMKYSADVERFEALATQHTEAVIAR
EHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
ISTRFNRVLILVCVGMVASVLLIVVVHLALRKLVVAPLHQACDLIMRVADGDLTIKVPEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
GRNEIGQLLRALSRMQHGLTDTVAKVRAGSDAVTTGAREIAAGNTDLSSRTEQQSSALEQ
CCHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHCHHHHHCCCCCHHHHHHHHHHHHHH
TAASMEELTSTVANNAESATRASGLARDAADLASRGGEVVRGVVQTMSEINASSQKIVDI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHH
IAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRALAQRSAGAAKEIKSLIDSS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHH
VDKVGAGSAQVEQAGSTIEDIVVAVRRLADTVNEISAASIEQSSGISQVSEAVSQMEQVN
HHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QQNAALVEQAAAAADSLESHANQLAAAVTTFKLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNNLSIRHGLLATLVIFGLMIVFGAALGVTQLYAASESAEDMHQVSAHVQLLNSAYKDMT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RARAALTRAYNSAKAGGGINAEAIGNAEGSIRKSTDALERFASAPVLEGLDASARDGMVA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHCCCCCCCCCCEEE
VARAHNDAVQRGLEALRKDDPDGFVAINDKDITATGMKYSADVERFEALATQHTEAVIAR
EHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
ISTRFNRVLILVCVGMVASVLLIVVVHLALRKLVVAPLHQACDLIMRVADGDLTIKVPEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
GRNEIGQLLRALSRMQHGLTDTVAKVRAGSDAVTTGAREIAAGNTDLSSRTEQQSSALEQ
CCHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHCHHHHHCCCCCHHHHHHHHHHHHHH
TAASMEELTSTVANNAESATRASGLARDAADLASRGGEVVRGVVQTMSEINASSQKIVDI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHH
IAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRALAQRSAGAAKEIKSLIDSS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHH
VDKVGAGSAQVEQAGSTIEDIVVAVRRLADTVNEISAASIEQSSGISQVSEAVSQMEQVN
HHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QQNAALVEQAAAAADSLESHANQLAAAVTTFKLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8419927; 11677609 [H]