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The map label for this gene is ispH [H]

Identifier: 94313096

GI number: 94313096

Start: 755356

End: 756354

Strand: Reverse

Name: ispH [H]

Synonym: Rmet_4169

Alternate gene names: 94313096

Gene position: 756354-755356 (Counterclockwise)

Preceding gene: 94313098

Following gene: 94313095

Centisome position: 29.32

GC content: 63.76

Gene sequence:

>999_bases
ATGCAGGTGATACTTGCTCAACCTCGCGGATTTTGCGCAGGTGTCGTCCGCGCGATCGAAATCGTCGATCGAGCCCTCGT
CAAGCATGGCGCCCCCGTCTATGTGCGACACGAAATCGTACACAACAAGCATGTGGTGGAAAGCCTGCGCCAGAAAGGCG
CGCGCTTTGTCGAGGAACTCGACGAGGTGCCTGGCAATGCAGTGACGATCTTCAGCGCGCATGGCGTGTCGCGGGACGTG
GTCAGCGAGGCCGCCCGCCGCGAACTGCACGCGATCGACGCGACCTGCCCGCTCGTCATCAAGGTTCACAATCAGGGACG
CCAGTACGCGGCAGCGGGCCGCCACGTCGTCCTGATCGGCCATGCCGGCCACCCCGAGGTGGAAGGCACGATGGGGCAGA
TTCCGGGCAAGGTCATCCTGGTCCAGAACGAGGCCGATGTCGCCACCCTGGACCTGCCGAATGACGCACCGGTCGCTTAC
ATCACACAGACCACGTTGTCCGTGGACGACACCCGTAATATCATTGCGGCGCTCGGCGCGCGGTTTACCAATCTGGTTGG
CCCGAGTACACGCGATATCTGCTACGCCACTCAGAACCGGCAGAGCGCGGTGCGCGAGTTGTCCACCCGCGTGGACGTCA
TCCTCGTGATTGGCGCCACCAACAGTTCCAACTCGAATCGCCTGCGCGAAATCGGCACCGAGAGCGGCGTACCGAGTTAC
CTGATCGCCGATGGCAGCGAGCTGGACCCCACGTGGGTCCGCGACGCCGCCGTCGTCGGCATTACCGCGGGTGCCTCGGC
ACCCGAAGAAATGGTGGAAGACGTCATTGCGGCCTTGCGCCGCCTTGGTCCGGTCGAAGTTTCGACCATGGAAGGGATCG
AGGAACATGCCGAATTCCGCCTGCCGGCCGAGCTGGACAATGTGAAGCAGGCCCCGGCGGCCAGTAGTACCACTGAACCC
AGCGACGCACGCCTTGCCGACCAGACGGCGGCAGGCTGA

Upstream 100 bases:

>100_bases
ACCGGTATATAGTAACCACCGTAGTCCGGAAATGTGATCCCGGGAATACCAAAATATAAATGTCTTCTAATCTTCACAAG
AGGGTCGGGGGGCCATTTCG

Downstream 100 bases:

>100_bases
AGCAACGAACCTAACAGGAAGCACCCCCTTGGCCATTCCCTTTCTGCAGGTCGCCCGCGTAGGCGCCTACATCGCACGCA
AGCATCTTTCCGGGCAGAAG

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MQVILAQPRGFCAGVVRAIEIVDRALVKHGAPVYVRHEIVHNKHVVESLRQKGARFVEELDEVPGNAVTIFSAHGVSRDV
VSEAARRELHAIDATCPLVIKVHNQGRQYAAAGRHVVLIGHAGHPEVEGTMGQIPGKVILVQNEADVATLDLPNDAPVAY
ITQTTLSVDDTRNIIAALGARFTNLVGPSTRDICYATQNRQSAVRELSTRVDVILVIGATNSSNSNRLREIGTESGVPSY
LIADGSELDPTWVRDAAVVGITAGASAPEEMVEDVIAALRRLGPVEVSTMEGIEEHAEFRLPAELDNVKQAPAASSTTEP
SDARLADQTAAG

Sequences:

>Translated_332_residues
MQVILAQPRGFCAGVVRAIEIVDRALVKHGAPVYVRHEIVHNKHVVESLRQKGARFVEELDEVPGNAVTIFSAHGVSRDV
VSEAARRELHAIDATCPLVIKVHNQGRQYAAAGRHVVLIGHAGHPEVEGTMGQIPGKVILVQNEADVATLDLPNDAPVAY
ITQTTLSVDDTRNIIAALGARFTNLVGPSTRDICYATQNRQSAVRELSTRVDVILVIGATNSSNSNRLREIGTESGVPSY
LIADGSELDPTWVRDAAVVGITAGASAPEEMVEDVIAALRRLGPVEVSTMEGIEEHAEFRLPAELDNVKQAPAASSTTEP
SDARLADQTAAG
>Mature_332_residues
MQVILAQPRGFCAGVVRAIEIVDRALVKHGAPVYVRHEIVHNKHVVESLRQKGARFVEELDEVPGNAVTIFSAHGVSRDV
VSEAARRELHAIDATCPLVIKVHNQGRQYAAAGRHVVLIGHAGHPEVEGTMGQIPGKVILVQNEADVATLDLPNDAPVAY
ITQTTLSVDDTRNIIAALGARFTNLVGPSTRDICYATQNRQSAVRELSTRVDVILVIGATNSSNSNRLREIGTESGVPSY
LIADGSELDPTWVRDAAVVGITAGASAPEEMVEDVIAALRRLGPVEVSTMEGIEEHAEFRLPAELDNVKQAPAASSTTEP
SDARLADQTAAG

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=308, Percent_Identity=53.2467532467532, Blast_Score=332, Evalue=1e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 35423; Mature: 35423

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVILAQPRGFCAGVVRAIEIVDRALVKHGAPVYVRHEIVHNKHVVESLRQKGARFVEEL
CEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
DEVPGNAVTIFSAHGVSRDVVSEAARRELHAIDATCPLVIKVHNQGRQYAAAGRHVVLIG
HHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEHHCCCEEEEEE
HAGHPEVEGTMGQIPGKVILVQNEADVATLDLPNDAPVAYITQTTLSVDDTRNIIAALGA
CCCCCCCCCHHHCCCCEEEEEECCCCEEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHH
RFTNLVGPSTRDICYATQNRQSAVRELSTRVDVILVIGATNSSNSNRLREIGTESGVPSY
HHHHHCCCCCCCEEEECCCHHHHHHHHHCCEEEEEEEECCCCCCCHHHHHHCCCCCCCCE
LIADGSELDPTWVRDAAVVGITAGASAPEEMVEDVIAALRRLGPVEVSTMEGIEEHAEFR
EEECCCCCCCHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCEE
LPAELDNVKQAPAASSTTEPSDARLADQTAAG
CCHHHHHHHCCCCCCCCCCCCCCHHCHHCCCC
>Mature Secondary Structure
MQVILAQPRGFCAGVVRAIEIVDRALVKHGAPVYVRHEIVHNKHVVESLRQKGARFVEEL
CEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
DEVPGNAVTIFSAHGVSRDVVSEAARRELHAIDATCPLVIKVHNQGRQYAAAGRHVVLIG
HHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEHHCCCEEEEEE
HAGHPEVEGTMGQIPGKVILVQNEADVATLDLPNDAPVAYITQTTLSVDDTRNIIAALGA
CCCCCCCCCHHHCCCCEEEEEECCCCEEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHH
RFTNLVGPSTRDICYATQNRQSAVRELSTRVDVILVIGATNSSNSNRLREIGTESGVPSY
HHHHHCCCCCCCEEEECCCHHHHHHHHHCCEEEEEEEECCCCCCCHHHHHHCCCCCCCCE
LIADGSELDPTWVRDAAVVGITAGASAPEEMVEDVIAALRRLGPVEVSTMEGIEEHAEFR
EEECCCCCCCHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCEE
LPAELDNVKQAPAASSTTEPSDARLADQTAAG
CCHHHHHHHCCCCCCCCCCCCCCHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA