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The map label for this gene is pdxK

Identifier: 94313041

GI number: 94313041

Start: 685379

End: 686245

Strand: Direct

Name: pdxK

Synonym: Rmet_4114

Alternate gene names: 94313041

Gene position: 685379-686245 (Clockwise)

Preceding gene: 94313040

Following gene: 94313042

Centisome position: 26.56

GC content: 64.82

Gene sequence:

>867_bases
ATGTTTGGTGAGACAGGTACCGGGGACGCGCTGCGCCCCATCTTCATGGACGTTGTGTCCGTACAGTCGCAGGTGGTCTA
TGGCCATGTTGGTAACAACGTGGCCTTGCCAACGTTGCGCGCGCATGGCCTGAACGTGGCCGCCGTGCCGACGGTCATGT
TCAGCAACACCCCTCACTACCCGACGCTGCACGGTGGAGCGCTACCCATCGACTGGTTCGGGGGATACCTGAGCGACCTG
TCGGCCCGTGGCGCGCTCCAGCGCCTGAAAGCCATCCTGGTCGGCTATCTCGGCAATCCGGAGCAGGTGGCCGTGCTCTC
CCGCTGGATCAATCAGGTGCTGGCCGAGCACCCGGATGTGCAGGTGATCGTCGACCCGGTGATCGGTGACCATGACACCG
GGATCTATGTCGCGGATGGCATGGTCGAGGCCGTGCGCGAGCTGCTTCTGCCGCTGGCACATGGACTCACGCCGAACGAT
TTCGAACTCGGGCATCTGAGTGGCCGTTCCGCCGACAGCGTCGAGCAGGTCGTCGCCGCCGCGCGCAGCCTGCTGACCGA
CCGCGTGCAATGGATGGTGGTCACCAGCGCGGCCCCCGGCACATGGGGACACGACGACATGAAACTGCTCATCGTCACCC
GGGACGATGCGCAGGTGCTGAGCCATCCCCGCATCCCGGTTTCGCCCAAGGGCACCGGAGACCTGTTCAGCGCGAAGTTG
ACCGCGCGGCTATTGGAAGGAATGCCGCTGGCCGAGGCCGCGGCAAGCGCCAGCGACCACGTGGTGACCGCTCTGGAAGC
CACCCGTCGCGCGCAAAGCCTGGAGCTTCAATTGCCATCGAACCCCTGTATCACGCATCGAGAGTGA

Upstream 100 bases:

>100_bases
AGAGGCGGAAGCGGGTGACTCTACATTGAGCGCTGCTCACTCCGGCCGTCAAGGCGAACGTTGATGGGACACGCTGCATT
GCATCGCAAGGAAATGAGCC

Downstream 100 bases:

>100_bases
TCCGATGAAAACCTATCGACTCAACATCTGGCGCCATGACAGGCTGCTGGGTCATTTCGATTCCGGCACGCCGTGGGCCG
CCGAGGCCGTCAAGGACGTT

Product: pyridoxal-pyridoxamine kinase/hydroxymethylpyrimidine kinase

Products: NA

Alternate protein names: PN/PL/PM kinase; Pyridoxal kinase; Pyridoxamine kinase; Vitamin B6 kinase

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MFGETGTGDALRPIFMDVVSVQSQVVYGHVGNNVALPTLRAHGLNVAAVPTVMFSNTPHYPTLHGGALPIDWFGGYLSDL
SARGALQRLKAILVGYLGNPEQVAVLSRWINQVLAEHPDVQVIVDPVIGDHDTGIYVADGMVEAVRELLLPLAHGLTPND
FELGHLSGRSADSVEQVVAAARSLLTDRVQWMVVTSAAPGTWGHDDMKLLIVTRDDAQVLSHPRIPVSPKGTGDLFSAKL
TARLLEGMPLAEAAASASDHVVTALEATRRAQSLELQLPSNPCITHRE

Sequences:

>Translated_288_residues
MFGETGTGDALRPIFMDVVSVQSQVVYGHVGNNVALPTLRAHGLNVAAVPTVMFSNTPHYPTLHGGALPIDWFGGYLSDL
SARGALQRLKAILVGYLGNPEQVAVLSRWINQVLAEHPDVQVIVDPVIGDHDTGIYVADGMVEAVRELLLPLAHGLTPND
FELGHLSGRSADSVEQVVAAARSLLTDRVQWMVVTSAAPGTWGHDDMKLLIVTRDDAQVLSHPRIPVSPKGTGDLFSAKL
TARLLEGMPLAEAAASASDHVVTALEATRRAQSLELQLPSNPCITHRE
>Mature_288_residues
MFGETGTGDALRPIFMDVVSVQSQVVYGHVGNNVALPTLRAHGLNVAAVPTVMFSNTPHYPTLHGGALPIDWFGGYLSDL
SARGALQRLKAILVGYLGNPEQVAVLSRWINQVLAEHPDVQVIVDPVIGDHDTGIYVADGMVEAVRELLLPLAHGLTPND
FELGHLSGRSADSVEQVVAAARSLLTDRVQWMVVTSAAPGTWGHDDMKLLIVTRDDAQVLSHPRIPVSPKGTGDLFSAKL
TARLLEGMPLAEAAASASDHVVTALEATRRAQSLELQLPSNPCITHRE

Specific function: Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates

COG id: COG2240

COG function: function code H; Pyridoxal/pyridoxine/pyridoxamine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyridoxine kinase family

Homologues:

Organism=Homo sapiens, GI4505701, Length=238, Percent_Identity=29.8319327731092, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1788758, Length=272, Percent_Identity=47.7941176470588, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI1787924, Length=268, Percent_Identity=32.8358208955224, Blast_Score=130, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17507759, Length=267, Percent_Identity=28.0898876404494, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17507757, Length=278, Percent_Identity=27.3381294964029, Blast_Score=95, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324354, Length=252, Percent_Identity=29.3650793650794, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320806, Length=266, Percent_Identity=26.6917293233083, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI45553007, Length=235, Percent_Identity=28.936170212766, Blast_Score=85, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXK_RALME (Q1LFU5)

Other databases:

- EMBL:   CP000353
- RefSeq:   YP_586250.1
- ProteinModelPortal:   Q1LFU5
- SMR:   Q1LFU5
- STRING:   Q1LFU5
- GeneID:   4040972
- GenomeReviews:   CP000353_GR
- KEGG:   rme:Rmet_4114
- eggNOG:   COG2240
- HOGENOM:   HBG661459
- OMA:   RARMLVS
- PhylomeDB:   Q1LFU5
- ProtClustDB:   PRK08176
- BioCyc:   RMET266264:RMET_4114-MONOMER
- HAMAP:   MF_01638
- InterPro:   IPR013749
- InterPro:   IPR004625
- TIGRFAMs:   TIGR00687

Pfam domain/function: PF08543 Phos_pyr_kin

EC number: =2.7.1.35

Molecular weight: Translated: 30800; Mature: 30800

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: BINDING 23-23 BINDING 136-136 BINDING 234-234

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGETGTGDALRPIFMDVVSVQSQVVYGHVGNNVALPTLRAHGLNVAAVPTVMFSNTPHY
CCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCEECCCHHHCCCCEEECCEEEECCCCCC
PTLHGGALPIDWFGGYLSDLSARGALQRLKAILVGYLGNPEQVAVLSRWINQVLAEHPDV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCE
QVIVDPVIGDHDTGIYVADGMVEAVRELLLPLAHGLTPNDFELGHLSGRSADSVEQVVAA
EEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHH
ARSLLTDRVQWMVVTSAAPGTWGHDDMKLLIVTRDDAQVLSHPRIPVSPKGTGDLFSAKL
HHHHHHHCEEEEEEEECCCCCCCCCCEEEEEEECCCHHHHCCCCCCCCCCCCCHHHHHHH
TARLLEGMPLAEAAASASDHVVTALEATRRAQSLELQLPSNPCITHRE
HHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCCCC
>Mature Secondary Structure
MFGETGTGDALRPIFMDVVSVQSQVVYGHVGNNVALPTLRAHGLNVAAVPTVMFSNTPHY
CCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCEECCCHHHCCCCEEECCEEEECCCCCC
PTLHGGALPIDWFGGYLSDLSARGALQRLKAILVGYLGNPEQVAVLSRWINQVLAEHPDV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCE
QVIVDPVIGDHDTGIYVADGMVEAVRELLLPLAHGLTPNDFELGHLSGRSADSVEQVVAA
EEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHH
ARSLLTDRVQWMVVTSAAPGTWGHDDMKLLIVTRDDAQVLSHPRIPVSPKGTGDLFSAKL
HHHHHHHCEEEEEEEECCCCCCCCCCEEEEEEECCCHHHHCCCCCCCCCCCCCHHHHHHH
TARLLEGMPLAEAAASASDHVVTALEATRRAQSLELQLPSNPCITHRE
HHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA