Click here to switch to the map view.

The map label for this gene is pobB [H]

Identifier: 94313039

GI number: 94313039

Start: 683359

End: 684312

Strand: Direct

Name: pobB [H]

Synonym: Rmet_4112

Alternate gene names: 94313039

Gene position: 683359-684312 (Clockwise)

Preceding gene: 94313038

Following gene: 94313040

Centisome position: 26.49

GC content: 66.14

Gene sequence:

>954_bases
ATGGCGGCCCGGCCCATCAAGACCGTTTCGGTGGTGGTGTCCGCCATCGACGAGCCACTGCCCGGCGTCAAGCGGTTCGT
CCTCAGCGACCAGGACCACTGGCCGTTGCCGCCTTTCACCCCCGGCGCGCATATCGACCTGCATCTCGGAGGCGGCCTGG
TGCGCACGTACTCGCTGTGCAACGAACCTTCGGACAGGAACCGGTATGTCATCGCCGTGAAGCACGAGGTGGCGGGGCGA
GGGGGATCGGCCTTCGTGCACGAGCGGCTCGATGTCGGGGCGTCGATCGGGGTGTCGCTGCCCAGAGGTGGCATCCGCAC
CACGGACACGGGGATGAACGTCTTCATCGCAGGCGGGATAGGTGTGACGCCGTTTATCTCGACGATCCGCGATCTCGAAC
TGCGGGGCCAGACGAACTACGTGCTGCACTGGTCCAGCGCGGGGGTGCCATCGCTCGTCGACATGCTGGGCGATGCCATC
GCGGCGGGCAGGGTCCGGCTTTACGACACACGCGTCGAATCCGCGCCCGATATGGATGCGATTCTGAATGCGTGCGGTGA
GAATGCCAAAGCCTACTGCTGCGGTCCGATGAGGATGCTTGACGCCTTCGAGCGGATCGTCGAAGGCTGGCCGGACGCCC
GCAAGCACATCGAGCGCTTCGCGCCGCCCAAGCCTGTGGAAGATACCGACGCGGCGCCTTATACCGTGGTGCTGGCAAGG
AGCGGGAAGGAGGCGATCGTCGAGCCACATGTCGGGCTGGTCGGAACGCTGGAGGCACTTGGGGCGGACGTGTCCGTGTC
TTGCGGAGGCGGCGTTTGTGGCGCGTGCAGGACGACCTGGCTGGAAGGGCCGCCGATCCATCGGGACCGCGTTCTGTCGC
CGGAAGAGCGCGCCCAGGACGTCATGGTCTGTGTGGCCGGATGCGCGGGGTCACGTCTGGTGCTGGATCTGTAG

Upstream 100 bases:

>100_bases
GCAAGGCTGTCTTCGATCCGCTTGTTCATCGCTCGGGTCTCAACTGCAAGATACTCAGCGGCGGCGTGGTGCGTATCGGC
GATGTAGTGAGGCCAGCCTG

Downstream 100 bases:

>100_bases
GGGCTGCCGCTTCGCCCCTGGGCCTGAGCCTGTGCCTGTGCATGGTTTCGGTTTTCGCCGGATCCCACTCCGTCCTGTGT
CGTGATAGCATTTGTCCTAT

Product: oxidoreductase/oxygenase, vanB family

Products: NA

Alternate protein names: 4-carboxydiphenyl ether; phenoxybenzoate dioxygenase subunit beta [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MAARPIKTVSVVVSAIDEPLPGVKRFVLSDQDHWPLPPFTPGAHIDLHLGGGLVRTYSLCNEPSDRNRYVIAVKHEVAGR
GGSAFVHERLDVGASIGVSLPRGGIRTTDTGMNVFIAGGIGVTPFISTIRDLELRGQTNYVLHWSSAGVPSLVDMLGDAI
AAGRVRLYDTRVESAPDMDAILNACGENAKAYCCGPMRMLDAFERIVEGWPDARKHIERFAPPKPVEDTDAAPYTVVLAR
SGKEAIVEPHVGLVGTLEALGADVSVSCGGGVCGACRTTWLEGPPIHRDRVLSPEERAQDVMVCVAGCAGSRLVLDL

Sequences:

>Translated_317_residues
MAARPIKTVSVVVSAIDEPLPGVKRFVLSDQDHWPLPPFTPGAHIDLHLGGGLVRTYSLCNEPSDRNRYVIAVKHEVAGR
GGSAFVHERLDVGASIGVSLPRGGIRTTDTGMNVFIAGGIGVTPFISTIRDLELRGQTNYVLHWSSAGVPSLVDMLGDAI
AAGRVRLYDTRVESAPDMDAILNACGENAKAYCCGPMRMLDAFERIVEGWPDARKHIERFAPPKPVEDTDAAPYTVVLAR
SGKEAIVEPHVGLVGTLEALGADVSVSCGGGVCGACRTTWLEGPPIHRDRVLSPEERAQDVMVCVAGCAGSRLVLDL
>Mature_316_residues
AARPIKTVSVVVSAIDEPLPGVKRFVLSDQDHWPLPPFTPGAHIDLHLGGGLVRTYSLCNEPSDRNRYVIAVKHEVAGRG
GSAFVHERLDVGASIGVSLPRGGIRTTDTGMNVFIAGGIGVTPFISTIRDLELRGQTNYVLHWSSAGVPSLVDMLGDAIA
AGRVRLYDTRVESAPDMDAILNACGENAKAYCCGPMRMLDAFERIVEGWPDARKHIERFAPPKPVEDTDAAPYTVVLARS
GKEAIVEPHVGLVGTLEALGADVSVSCGGGVCGACRTTWLEGPPIHRDRVLSPEERAQDVMVCVAGCAGSRLVLDL

Specific function: Degrades exclusively diarylether compounds having carboxyl groups in the 3- or 4-position. Yields a hemiacetal that spontaneously hydrolyzes to phenol and protocatechuate [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=322, Percent_Identity=34.472049689441, Blast_Score=166, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 33766; Mature: 33635

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00197 2FE2S_FER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAARPIKTVSVVVSAIDEPLPGVKRFVLSDQDHWPLPPFTPGAHIDLHLGGGLVRTYSLC
CCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHH
NEPSDRNRYVIAVKHEVAGRGGSAFVHERLDVGASIGVSLPRGGIRTTDTGMNVFIAGGI
CCCCCCCEEEEEEEHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEECCCCEEEEECCC
GVTPFISTIRDLELRGQTNYVLHWSSAGVPSLVDMLGDAIAAGRVRLYDTRVESAPDMDA
CHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCEEEEEECCCCCCCCHHH
ILNACGENAKAYCCGPMRMLDAFERIVEGWPDARKHIERFAPPKPVEDTDAAPYTVVLAR
HHHHCCCCCCEEEECHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEC
SGKEAIVEPHVGLVGTLEALGADVSVSCGGGVCGACRTTWLEGPPIHRDRVLSPEERAQD
CCCCEEECCCCCCEEEHHHHCCCEEEECCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHH
VMVCVAGCAGSRLVLDL
EEEEEECCCCCEEEEEC
>Mature Secondary Structure 
AARPIKTVSVVVSAIDEPLPGVKRFVLSDQDHWPLPPFTPGAHIDLHLGGGLVRTYSLC
CCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHH
NEPSDRNRYVIAVKHEVAGRGGSAFVHERLDVGASIGVSLPRGGIRTTDTGMNVFIAGGI
CCCCCCCEEEEEEEHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEECCCCEEEEECCC
GVTPFISTIRDLELRGQTNYVLHWSSAGVPSLVDMLGDAIAAGRVRLYDTRVESAPDMDA
CHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCEEEEEECCCCCCCCHHH
ILNACGENAKAYCCGPMRMLDAFERIVEGWPDARKHIERFAPPKPVEDTDAAPYTVVLAR
HHHHCCCCCCEEEECHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEC
SGKEAIVEPHVGLVGTLEALGADVSVSCGGGVCGACRTTWLEGPPIHRDRVLSPEERAQD
CCCCEEECCCCCCEEEHHHHCCCEEEECCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHH
VMVCVAGCAGSRLVLDL
EEEEEECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7710319 [H]