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The map label for this gene is fadH [C]

Identifier: 94313018

GI number: 94313018

Start: 659772

End: 661034

Strand: Direct

Name: fadH [C]

Synonym: Rmet_4091

Alternate gene names: 94313018

Gene position: 659772-661034 (Clockwise)

Preceding gene: 94313013

Following gene: 94313020

Centisome position: 25.57

GC content: 65.8

Gene sequence:

>1263_bases
ATGCGCAAATGCCTGCGAACCCCAGCCATGAAACTGTTCACGCCCCTTACCCTGCCCAACGGCGCAGTCATTCCCAACCG
CCTGGCCAAGGCCGCCATGGAAGAGAACATGGCCGATGCGGACCATGCGCCATCTGACGCATTGCTACGCCTCTACGAAG
CCTGGGCCGCGGGAGGCGCTGGCCTGATGCTTACGGGCAATGTCATGGTCGATCACCGGGCGATGACCGGACCGAATGGC
GTCGTGCTGGAGAACGACGCCCATCTGGATCGCTTCCGGCAATGGGCGCGGATCGCGCGCGCCCACGGCGCCCACGTCTG
GATGCAGATCAACCACCCCGGCCGCCAGATGCCCGCCGCGCTGGGACAGACCACGCTGGCGCCGTCAGCGGTTCCGCTGG
AACTGGGCGCGCTGTCCAAGCAGTTTCCTGTTCCCAGGGAGATGACCGCAGCCGACATCGACGATGTGCGCGAGCTCTTC
GTCCGCAGCGCTCAGCTGGCCGAACAGAGCGGCTTCACTGGCGTGCAGATCCACGCGGCCCATGGCTATCTGCTCAGTCA
GTTCCTGTCGCCGCTGGCTAACCGGCGACAGGACCAATGGGGTGGCCCGATCGAGAACCGCGCGCGCCTGCTGCTCGACA
TCGTACGTGCGGTGCGGCAGGCGGTGTCGCCGGCTTTCGTGGTGTCCGTCAAGCTGAACTCGGCCGATTTCCAGCGCGGG
GGATTCAGCCCCGATGACGCGAAGCGCGTGGTCGAACTGCTCAATCCGCTCGGCGTCGATCTGGTGGAACTGTCGGGCGG
CAGCTACGAAGCCCCGGCCATGCAAGGGCAGGCGCGGGATGGGCGCACGCTCGAGCGCGAGGCGTACTTTCTGGCCTTTG
CGCGTGATATCGCAGCCATCGCGCAGATGCCGCTGATGGTCACGGGTGGTATCCGTCGCCGCGCCGTGGCCGACCAGGTG
GTCGATAGCGGCATTGCGATGGTTGGCATCGCCACCGCGCTGTCCATCGATCCCAATCTGCCGCGCGATTGGTTGGCGGG
CAAGAACAACGCGCCCGCGCTCCGCCCGATCACATGGAAGAACAAGGTGCTGGGTTCGCTGGCCAACATGGCCGTGGTCA
AGTTCCAGCTCAAGCGGCTCAGCCAGGGACGCGGCACGCATCCACGGGTCTCCCCGCTCAGGGCACTGCTCATGCAGCAA
ATGGCCAACGCCTGCCAGACAAGGCGTTACAAGCGCTGGATGATGGGGCGGGAGTTGGGCTGA

Upstream 100 bases:

>100_bases
CCGCTCAGGCGTGCCAGTTCTCCAATCTTCATCTGCAGTGTTCCAGGGGACTTGACATTAAAGTTGACTTTAAGCTTAGG
GTTACGCAGTCGTCAACGCA

Downstream 100 bases:

>100_bases
GGGTAAGTGAGGGTAAGGGGCAGGCCCGCTATCGCCCCTGATTCACGGCTTCGAATCCGGCGACGAGTGCATCGAGCAGA
TGGCGCACGGCGGGCACCAT

Product: flavine mononucleotide (FMN) oxidoreductase-like FMN binding protein

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MRKCLRTPAMKLFTPLTLPNGAVIPNRLAKAAMEENMADADHAPSDALLRLYEAWAAGGAGLMLTGNVMVDHRAMTGPNG
VVLENDAHLDRFRQWARIARAHGAHVWMQINHPGRQMPAALGQTTLAPSAVPLELGALSKQFPVPREMTAADIDDVRELF
VRSAQLAEQSGFTGVQIHAAHGYLLSQFLSPLANRRQDQWGGPIENRARLLLDIVRAVRQAVSPAFVVSVKLNSADFQRG
GFSPDDAKRVVELLNPLGVDLVELSGGSYEAPAMQGQARDGRTLEREAYFLAFARDIAAIAQMPLMVTGGIRRRAVADQV
VDSGIAMVGIATALSIDPNLPRDWLAGKNNAPALRPITWKNKVLGSLANMAVVKFQLKRLSQGRGTHPRVSPLRALLMQQ
MANACQTRRYKRWMMGRELG

Sequences:

>Translated_420_residues
MRKCLRTPAMKLFTPLTLPNGAVIPNRLAKAAMEENMADADHAPSDALLRLYEAWAAGGAGLMLTGNVMVDHRAMTGPNG
VVLENDAHLDRFRQWARIARAHGAHVWMQINHPGRQMPAALGQTTLAPSAVPLELGALSKQFPVPREMTAADIDDVRELF
VRSAQLAEQSGFTGVQIHAAHGYLLSQFLSPLANRRQDQWGGPIENRARLLLDIVRAVRQAVSPAFVVSVKLNSADFQRG
GFSPDDAKRVVELLNPLGVDLVELSGGSYEAPAMQGQARDGRTLEREAYFLAFARDIAAIAQMPLMVTGGIRRRAVADQV
VDSGIAMVGIATALSIDPNLPRDWLAGKNNAPALRPITWKNKVLGSLANMAVVKFQLKRLSQGRGTHPRVSPLRALLMQQ
MANACQTRRYKRWMMGRELG
>Mature_420_residues
MRKCLRTPAMKLFTPLTLPNGAVIPNRLAKAAMEENMADADHAPSDALLRLYEAWAAGGAGLMLTGNVMVDHRAMTGPNG
VVLENDAHLDRFRQWARIARAHGAHVWMQINHPGRQMPAALGQTTLAPSAVPLELGALSKQFPVPREMTAADIDDVRELF
VRSAQLAEQSGFTGVQIHAAHGYLLSQFLSPLANRRQDQWGGPIENRARLLLDIVRAVRQAVSPAFVVSVKLNSADFQRG
GFSPDDAKRVVELLNPLGVDLVELSGGSYEAPAMQGQARDGRTLEREAYFLAFARDIAAIAQMPLMVTGGIRRRAVADQV
VDSGIAMVGIATALSIDPNLPRDWLAGKNNAPALRPITWKNKVLGSLANMAVVKFQLKRLSQGRGTHPRVSPLRALLMQQ
MANACQTRRYKRWMMGRELG

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=333, Percent_Identity=27.3273273273273, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787939, Length=273, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17559802, Length=416, Percent_Identity=32.9326923076923, Blast_Score=214, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI17565138, Length=346, Percent_Identity=34.393063583815, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17564188, Length=365, Percent_Identity=33.6986301369863, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI72001454, Length=351, Percent_Identity=34.1880341880342, Blast_Score=204, Evalue=8e-53,
Organism=Caenorhabditis elegans, GI17559804, Length=365, Percent_Identity=32.3287671232877, Blast_Score=199, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17540738, Length=348, Percent_Identity=33.0459770114943, Blast_Score=198, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17566914, Length=355, Percent_Identity=35.2112676056338, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17566916, Length=353, Percent_Identity=32.5779036827195, Blast_Score=150, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6321973, Length=80, Percent_Identity=45, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325086, Length=80, Percent_Identity=42.5, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 45888; Mature: 45888

Theoretical pI: Translated: 10.81; Mature: 10.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKCLRTPAMKLFTPLTLPNGAVIPNRLAKAAMEENMADADHAPSDALLRLYEAWAAGGA
CCHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCC
GLMLTGNVMVDHRAMTGPNGVVLENDAHLDRFRQWARIARAHGAHVWMQINHPGRQMPAA
CEEEECCEEEECCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHH
LGQTTLAPSAVPLELGALSKQFPVPREMTAADIDDVRELFVRSAQLAEQSGFTGVQIHAA
HCCCCCCCCCCCHHHHHCHHCCCCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCEEEEECH
HGYLLSQFLSPLANRRQDQWGGPIENRARLLLDIVRAVRQAVSPAFVVSVKLNSADFQRG
HHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCC
GFSPDDAKRVVELLNPLGVDLVELSGGSYEAPAMQGQARDGRTLEREAYFLAFARDIAAI
CCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
AQMPLMVTGGIRRRAVADQVVDSGIAMVGIATALSIDPNLPRDWLAGKNNAPALRPITWK
HHCCHHEECCHHHHHHHHHHHHCCHHHHHHHHHEECCCCCCHHHHCCCCCCCCCCCCCHH
NKVLGSLANMAVVKFQLKRLSQGRGTHPRVSPLRALLMQQMANACQTRRYKRWMMGRELG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRKCLRTPAMKLFTPLTLPNGAVIPNRLAKAAMEENMADADHAPSDALLRLYEAWAAGGA
CCHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCC
GLMLTGNVMVDHRAMTGPNGVVLENDAHLDRFRQWARIARAHGAHVWMQINHPGRQMPAA
CEEEECCEEEECCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHH
LGQTTLAPSAVPLELGALSKQFPVPREMTAADIDDVRELFVRSAQLAEQSGFTGVQIHAA
HCCCCCCCCCCCHHHHHCHHCCCCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCEEEEECH
HGYLLSQFLSPLANRRQDQWGGPIENRARLLLDIVRAVRQAVSPAFVVSVKLNSADFQRG
HHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCC
GFSPDDAKRVVELLNPLGVDLVELSGGSYEAPAMQGQARDGRTLEREAYFLAFARDIAAI
CCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
AQMPLMVTGGIRRRAVADQVVDSGIAMVGIATALSIDPNLPRDWLAGKNNAPALRPITWK
HHCCHHEECCHHHHHHHHHHHHCCHHHHHHHHHEECCCCCCHHHHCCCCCCCCCCCCCHH
NKVLGSLANMAVVKFQLKRLSQGRGTHPRVSPLRALLMQQMANACQTRRYKRWMMGRELG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]