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The map label for this gene is alsR [H]

Identifier: 94312998

GI number: 94312998

Start: 638132

End: 639025

Strand: Reverse

Name: alsR [H]

Synonym: Rmet_4070

Alternate gene names: 94312998

Gene position: 639025-638132 (Counterclockwise)

Preceding gene: 94313004

Following gene: 94312997

Centisome position: 24.77

GC content: 67.34

Gene sequence:

>894_bases
ATGGATCTACGTCAGCTCCAGCAATTCGTCGTGCTGGCCGAAACCGGCAACTTCCACCGCGCCGCCGAGCGCCTGCACAT
GGCGCAACCCCCGTTGTCGATTTCGATCCGCAAGCTGGAGGAATCACTGGGCACGCCGCTGTTTGTACGCACCACGCGCG
GCGTGCGGCTCACCCAGGCGGGCGAAGCGGCGCTCAACGATGCGCGCCGGGCCCTGTTCCATGCCGGCCAGGCGCGGGCC
GCAGCGGTGTCTGCGGCGCTTGGGGAACGCGGCGCGCTGCGTATCGGTTTTGTCGGCTCCGCCACCTATGCGTTGCTTCC
CCGTCTCATCCCGGCCTTCCGCTCGGCACACCCTGGCATCGACCTGATCCTTCACGAATCCACGTCGGCCGCGATTCTGG
ACCGCATCGAACACGGCCAGCTCGACGCCGGACTCGTACGCTTTCCCATCCTCAACAGCGGCCCTTACACGTTGACCCCG
CTCGAGTCCGACGCGTTCGTGGCGGCCGTGCCGGCCGACGGGCGCTTTGCCGAGCAGGAGAGCATCGCGTTGAAGGCACT
GGCCGACGAGCCCTTCATCATGCATCCCCGTGCCGATGTGCCGAACCTGCAGGCCGTGGCGATGCTGCTGTGCCAGCAGG
CCGGTTTTGTGCCGCGCATCGCGCAGGAAGCGGTGCAGGTGCAGACCATCGTCAGTCTGGTGGAAAGCGGATTGGGCGTC
GCGCTGGTGCCTGGCGTGGCGGCGCGTTACACGAATCGGCGCGTGCGCTTTCTGCGCCTGTCCAGCCCACGCCCGGCGGC
ACGTATCGGCATCGCCCTGGCCACGCAGCAGGACACCGACGATCGCCATGTGCAGCGCTTTATCGCTGCAGCGCAGCGAA
TCGCAGGCGACTAG

Upstream 100 bases:

>100_bases
GTTGTATTGATTCGATGTATTGATCGGCCAAGATTAAGGCAGCCAGCCCCATTGCGTCTAATATTAGATTTTTAGCAACC
GATATTCACTGGATATCATC

Downstream 100 bases:

>100_bases
GCGCGGCGGCAGAAGGCAACTCAAGGCAACTCAAGGCAGCAATTCCGCAGGGCGGCTGCGGCTATACAGCCGATGCATGG
CCGCCATGAAACTTGCGCCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Als operon regulatory protein [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MDLRQLQQFVVLAETGNFHRAAERLHMAQPPLSISIRKLEESLGTPLFVRTTRGVRLTQAGEAALNDARRALFHAGQARA
AAVSAALGERGALRIGFVGSATYALLPRLIPAFRSAHPGIDLILHESTSAAILDRIEHGQLDAGLVRFPILNSGPYTLTP
LESDAFVAAVPADGRFAEQESIALKALADEPFIMHPRADVPNLQAVAMLLCQQAGFVPRIAQEAVQVQTIVSLVESGLGV
ALVPGVAARYTNRRVRFLRLSSPRPAARIGIALATQQDTDDRHVQRFIAAAQRIAGD

Sequences:

>Translated_297_residues
MDLRQLQQFVVLAETGNFHRAAERLHMAQPPLSISIRKLEESLGTPLFVRTTRGVRLTQAGEAALNDARRALFHAGQARA
AAVSAALGERGALRIGFVGSATYALLPRLIPAFRSAHPGIDLILHESTSAAILDRIEHGQLDAGLVRFPILNSGPYTLTP
LESDAFVAAVPADGRFAEQESIALKALADEPFIMHPRADVPNLQAVAMLLCQQAGFVPRIAQEAVQVQTIVSLVESGLGV
ALVPGVAARYTNRRVRFLRLSSPRPAARIGIALATQQDTDDRHVQRFIAAAQRIAGD
>Mature_297_residues
MDLRQLQQFVVLAETGNFHRAAERLHMAQPPLSISIRKLEESLGTPLFVRTTRGVRLTQAGEAALNDARRALFHAGQARA
AAVSAALGERGALRIGFVGSATYALLPRLIPAFRSAHPGIDLILHESTSAAILDRIEHGQLDAGLVRFPILNSGPYTLTP
LESDAFVAAVPADGRFAEQESIALKALADEPFIMHPRADVPNLQAVAMLLCQQAGFVPRIAQEAVQVQTIVSLVESGLGV
ALVPGVAARYTNRRVRFLRLSSPRPAARIGIALATQQDTDDRHVQRFIAAAQRIAGD

Specific function: Regulates the expression of the alsSD operon for acetoin biosynthesis [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI2367136, Length=280, Percent_Identity=35, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI1788887, Length=293, Percent_Identity=33.7883959044369, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1790399, Length=273, Percent_Identity=31.5018315018315, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787879, Length=261, Percent_Identity=34.0996168582375, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI157672245, Length=245, Percent_Identity=33.469387755102, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI145693105, Length=249, Percent_Identity=28.9156626506024, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790208, Length=257, Percent_Identity=31.5175097276265, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788297, Length=254, Percent_Identity=29.1338582677165, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788748, Length=258, Percent_Identity=26.3565891472868, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787530, Length=249, Percent_Identity=24.8995983935743, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787601, Length=158, Percent_Identity=31.6455696202532, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87081904, Length=279, Percent_Identity=28.3154121863799, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787806, Length=150, Percent_Identity=31.3333333333333, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787589, Length=123, Percent_Identity=39.0243902439024, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786401, Length=158, Percent_Identity=33.5443037974684, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1786713, Length=72, Percent_Identity=48.6111111111111, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788296, Length=246, Percent_Identity=27.6422764227642, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31974; Mature: 31974

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRQLQQFVVLAETGNFHRAAERLHMAQPPLSISIRKLEESLGTPLFVRTTRGVRLTQA
CCHHHHHHHEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCEEEECC
GEAALNDARRALFHAGQARAAAVSAALGERGALRIGFVGSATYALLPRLIPAFRSAHPGI
CHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCE
DLILHESTSAAILDRIEHGQLDAGLVRFPILNSGPYTLTPLESDAFVAAVPADGRFAEQE
EEEEECCCHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEECCCCCEEEEECCCCCCCCHH
SIALKALADEPFIMHPRADVPNLQAVAMLLCQQAGFVPRIAQEAVQVQTIVSLVESGLGV
HHHHEEECCCCEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCE
ALVPGVAARYTNRRVRFLRLSSPRPAARIGIALATQQDTDDRHVQRFIAAAQRIAGD
EECCCHHHHHCCCEEEEEEECCCCCHHHEEEEEEECCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLRQLQQFVVLAETGNFHRAAERLHMAQPPLSISIRKLEESLGTPLFVRTTRGVRLTQA
CCHHHHHHHEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCEEEECC
GEAALNDARRALFHAGQARAAAVSAALGERGALRIGFVGSATYALLPRLIPAFRSAHPGI
CHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCE
DLILHESTSAAILDRIEHGQLDAGLVRFPILNSGPYTLTPLESDAFVAAVPADGRFAEQE
EEEEECCCHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEECCCCCEEEEECCCCCCCCHH
SIALKALADEPFIMHPRADVPNLQAVAMLLCQQAGFVPRIAQEAVQVQTIVSLVESGLGV
HHHHEEECCCCEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCE
ALVPGVAARYTNRRVRFLRLSSPRPAARIGIALATQQDTDDRHVQRFIAAAQRIAGD
EECCCHHHHHCCCEEEEEEECCCCCHHHEEEEEEECCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377 [H]