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The map label for this gene is pcaB [H]

Identifier: 94312943

GI number: 94312943

Start: 584937

End: 586409

Strand: Direct

Name: pcaB [H]

Synonym: Rmet_4015

Alternate gene names: 94312943

Gene position: 584937-586409 (Clockwise)

Preceding gene: 94312942

Following gene: 94312944

Centisome position: 22.67

GC content: 68.36

Gene sequence:

>1473_bases
ATGCACTGCAAGCAGGGCGCGGGGTTTCCCCCGTGCCCTGCTTTGCATGACAATCGGCGCATTACCACCGGTTTGCCCCC
ACCGACGATGCCCACGCTTTCTCGCCTGACCGACCCGATGTTCGGCAGTCCCGCCGTGCTCGAGGTCTTCTCCGATACTG
GCACCGTACGCCGGATGCTCGATGCCGAGGCCGCGCTCGCGCGTGCCGAAGCGCAGTGCGGCGTGATTCCCGCCACTGCC
GCCGCCGTCATTACTGAAGTCTGCGGCAGGGTCGATGTGATCGACCTCGAGGCGCTGGCGCAGGCGGCAGTATCCGCCGG
CAATCTCGCCATCCCGTTCGTCAAGCAATTAACTGCAGCCGTCACCCAGCGCGACGCCGACGCTGGCCGCTACGTGCATT
GGGGCGCAACCAGCCAGGACATCATCGATACCGCGCTCGTGCTGCAACTGCGCGATGCCCTGCGCGAGATCGTCGTCGAT
TTGCTGCGCGCGGGCGATGCCTGCGCGACGCTGGCCGCGACCCATCGCGCCACGCCCATGGTGGCACGCACATGGCTGCA
ACATGCGCTGCCCACCACGTTTGGCTGCAAGGCCGGCGGCTGGCTCGATGCCTTGCGTCGCGATCTGACGCGGCTAGACC
ACGTTGGGGCGCAGGTCCGCGTGATCCAGTTCGGTGGCGCCGCCGGCACCCTTGCCAGCCTGGGCGATGACGCGCCGGCC
GTGGCGGCCCGCTTCGCCGCCGAACTCCAGCTCGATGTGCCCGCGCTGCCGTGGCACGCGCATCGCGATCGTCTGGTCGA
AGTGGCCACCGCGCTCGGTATGTTGACCGGCACGCTGGGCAAGATGGCACGCGACGTCTCGCTGATGATGCAGACCGAGG
TTGGCGAGGTAGCCGAGCCATCTGGCCCGGGACGCGGTGGATCGAGCACGATGCCCCACAAGCGCAACCCGGTGGGATGC
GCGGCGGTGCTGACCGCGGCGACGCGTGTGCCACCGCTGGTGGCGACGATGCTTGCGGGCATGACGCAGGAGCATGAGCG
TGCGCTCGGTGGCTGGCAGGCCGAATGGGACACGCTGCCGCAGATCGTCACGCTGGCGGCAGGCGCGCTGCGCCAGATGG
CAGACGTCGCGTCCGGGCTGGAGGTCGATGAGGCCCGTATGCGCGCGAACCTCGATATCACGCACGGCCTGATCCTCGGT
GAGGCAACCATGCTCGAACTGGGGCGGCGCATCGGCCGGTTGCAGGCTCACCATCTCGTGGAGCAGGCTTCGCGCCGCGC
GGCGGCGGAAGACACCACGCTGCGTATCGCGCTGGCCCGCACGCTGGCCGAAGATGCGAATCACGCGGGATTGCTCGACG
ACGCCGCGCTCGACCGACTGAGCGATCCGGCGCAGTACGCTGGACAATCCGAACGTTTCACCGATGCCGCCGTTGCCGCA
TGGCAGGCGCTACGCGCGAACCCACAGAACTAA

Upstream 100 bases:

>100_bases
CGCGCCACGCTGATCGCGCAGCCGAACGGCCAGAACAGCTACCGCTGGGACGTCGTGCTGCAAGGCGCGGGCGAGACCGT
GTTCTTCGATATCTGATCTG

Downstream 100 bases:

>100_bases
GAGAACCAAGACCAGGCCCGACAGGAGACTCCCTTGCCTTACCTCGATCAAGCCGGCGCCCGGCTGTTCTACACCGTCGA
CGGACCGGAAGACGCACCGG

Product: 3-carboxy-cis,cis-muconate cycloisomerase

Products: NA

Alternate protein names: 3-carboxymuconate lactonizing enzyme; CMLE [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MHCKQGAGFPPCPALHDNRRITTGLPPPTMPTLSRLTDPMFGSPAVLEVFSDTGTVRRMLDAEAALARAEAQCGVIPATA
AAVITEVCGRVDVIDLEALAQAAVSAGNLAIPFVKQLTAAVTQRDADAGRYVHWGATSQDIIDTALVLQLRDALREIVVD
LLRAGDACATLAATHRATPMVARTWLQHALPTTFGCKAGGWLDALRRDLTRLDHVGAQVRVIQFGGAAGTLASLGDDAPA
VAARFAAELQLDVPALPWHAHRDRLVEVATALGMLTGTLGKMARDVSLMMQTEVGEVAEPSGPGRGGSSTMPHKRNPVGC
AAVLTAATRVPPLVATMLAGMTQEHERALGGWQAEWDTLPQIVTLAAGALRQMADVASGLEVDEARMRANLDITHGLILG
EATMLELGRRIGRLQAHHLVEQASRRAAAEDTTLRIALARTLAEDANHAGLLDDAALDRLSDPAQYAGQSERFTDAAVAA
WQALRANPQN

Sequences:

>Translated_490_residues
MHCKQGAGFPPCPALHDNRRITTGLPPPTMPTLSRLTDPMFGSPAVLEVFSDTGTVRRMLDAEAALARAEAQCGVIPATA
AAVITEVCGRVDVIDLEALAQAAVSAGNLAIPFVKQLTAAVTQRDADAGRYVHWGATSQDIIDTALVLQLRDALREIVVD
LLRAGDACATLAATHRATPMVARTWLQHALPTTFGCKAGGWLDALRRDLTRLDHVGAQVRVIQFGGAAGTLASLGDDAPA
VAARFAAELQLDVPALPWHAHRDRLVEVATALGMLTGTLGKMARDVSLMMQTEVGEVAEPSGPGRGGSSTMPHKRNPVGC
AAVLTAATRVPPLVATMLAGMTQEHERALGGWQAEWDTLPQIVTLAAGALRQMADVASGLEVDEARMRANLDITHGLILG
EATMLELGRRIGRLQAHHLVEQASRRAAAEDTTLRIALARTLAEDANHAGLLDDAALDRLSDPAQYAGQSERFTDAAVAA
WQALRANPQN
>Mature_490_residues
MHCKQGAGFPPCPALHDNRRITTGLPPPTMPTLSRLTDPMFGSPAVLEVFSDTGTVRRMLDAEAALARAEAQCGVIPATA
AAVITEVCGRVDVIDLEALAQAAVSAGNLAIPFVKQLTAAVTQRDADAGRYVHWGATSQDIIDTALVLQLRDALREIVVD
LLRAGDACATLAATHRATPMVARTWLQHALPTTFGCKAGGWLDALRRDLTRLDHVGAQVRVIQFGGAAGTLASLGDDAPA
VAARFAAELQLDVPALPWHAHRDRLVEVATALGMLTGTLGKMARDVSLMMQTEVGEVAEPSGPGRGGSSTMPHKRNPVGC
AAVLTAATRVPPLVATMLAGMTQEHERALGGWQAEWDTLPQIVTLAAGALRQMADVASGLEVDEARMRANLDITHGLILG
EATMLELGRRIGRLQAHHLVEQASRRAAAEDTTLRIALARTLAEDANHAGLLDDAALDRLSDPAQYAGQSERFTDAAVAA
WQALRANPQN

Specific function: Catalyzes an anti cycloisomerization [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI17508577, Length=369, Percent_Identity=23.3062330623306, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323391, Length=371, Percent_Identity=25.8760107816712, Blast_Score=87, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR012789 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =5.5.1.2 [H]

Molecular weight: Translated: 51782; Mature: 51782

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHCKQGAGFPPCPALHDNRRITTGLPPPTMPTLSRLTDPMFGSPAVLEVFSDTGTVRRML
CCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHCCCCCCCCHHHHHHHCCHHHHHHH
DAEAALARAEAQCGVIPATAAAVITEVCGRVDVIDLEALAQAAVSAGNLAIPFVKQLTAA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEECHHHHHHHHHCCCCCCHHHHHHHHHH
VTQRDADAGRYVHWGATSQDIIDTALVLQLRDALREIVVDLLRAGDACATLAATHRATPM
HHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHH
VARTWLQHALPTTFGCKAGGWLDALRRDLTRLDHVGAQVRVIQFGGAAGTLASLGDDAPA
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCCHH
VAARFAAELQLDVPALPWHAHRDRLVEVATALGMLTGTLGKMARDVSLMMQTEVGEVAEP
HHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SGPGRGGSSTMPHKRNPVGCAAVLTAATRVPPLVATMLAGMTQEHERALGGWQAEWDTLP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
QIVTLAAGALRQMADVASGLEVDEARMRANLDITHGLILGEATMLELGRRIGRLQAHHLV
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQASRRAAAEDTTLRIALARTLAEDANHAGLLDDAALDRLSDPAQYAGQSERFTDAAVAA
HHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHCCCCCHHHHHHHHH
WQALRANPQN
HHHHHCCCCC
>Mature Secondary Structure
MHCKQGAGFPPCPALHDNRRITTGLPPPTMPTLSRLTDPMFGSPAVLEVFSDTGTVRRML
CCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHCCCCCCCCHHHHHHHCCHHHHHHH
DAEAALARAEAQCGVIPATAAAVITEVCGRVDVIDLEALAQAAVSAGNLAIPFVKQLTAA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEECHHHHHHHHHCCCCCCHHHHHHHHHH
VTQRDADAGRYVHWGATSQDIIDTALVLQLRDALREIVVDLLRAGDACATLAATHRATPM
HHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHH
VARTWLQHALPTTFGCKAGGWLDALRRDLTRLDHVGAQVRVIQFGGAAGTLASLGDDAPA
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCCHH
VAARFAAELQLDVPALPWHAHRDRLVEVATALGMLTGTLGKMARDVSLMMQTEVGEVAEP
HHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SGPGRGGSSTMPHKRNPVGCAAVLTAATRVPPLVATMLAGMTQEHERALGGWQAEWDTLP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
QIVTLAAGALRQMADVASGLEVDEARMRANLDITHGLILGEATMLELGRRIGRLQAHHLV
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQASRRAAAEDTTLRIALARTLAEDANHAGLLDDAALDRLSDPAQYAGQSERFTDAAVAA
HHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHCCCCCHHHHHHHHH
WQALRANPQN
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8063101 [H]