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The map label for this gene is tnpA

Identifier: 94312932

GI number: 94312932

Start: 576113

End: 577132

Strand: Direct

Name: tnpA

Synonym: Rmet_4004

Alternate gene names: 94312932

Gene position: 576113-577132 (Clockwise)

Preceding gene: 94312931

Following gene: 94312933

Centisome position: 22.33

GC content: 64.51

Gene sequence:

>1020_bases
ATGACGAGAACGAAGTACCAACAACTACAACCCGAAGAACGCATGCGCATCGAGATTTGGAAGGCAGAAGATGTCAGCCT
GCGGGCCATGGCCCGCAGGCTTGGCCGCGCGCCTTCGACACTGATGCGTGAGCTTCGCCGCAATGCCACCGCCCGTGGCG
GCTATGGCGCAATGAGCGCACAAGCCTGCCGTACGCAACGCCTCAAGGCCAGCCGTCCGGTCGCTAAGCTCGCTCCTGAT
GGCGTATTGTGGGGCGTGGTGCGCCACTTCCTCGATCAGAAGTGGTCGCCCCAGGAAATCTCCGCTACGCTCAAGCGGGC
CTTTCCTGACCAACCCGACCTCAACGTGTCCCACGAGACCATCTACAACGCCATCTACGCATACCCGCGGGGTGAGCTGC
GCCGCCAGCTCATCGCCTGCTTGCGACAGGCCCGAACCAAGCGTCTGCCGCGCTCGCGTGGAACCGACCGGCGCGGCCAG
ATTCCCGACATGGTCAGCATTCACGTGCGCCCGCCCGAGGTGAACGACAGGCTGATGCCCGGGCACTGGGAGGGCGACCT
CATCAAGGGGGCGGGCAACCAATCCGCCGTAGGCGTGCTGGTTGAGCGCATGAGCCGCGCCGTACTGCTGGTCAAGATGC
CAGACGCCACCGCTGCATCGGCTCTGGCCGGCTTTACCGGGAAGCTGCAATCCCTGGTGGCGCCGCTGCGCCAGACCCTG
ACCTACGACCAGGGTCGAGAGATGGCCAGGCACGCCGAACTGAGCGCCGCCACTGGCGTGCGAGTGTACTTCTGCGACCC
GCACAGCCCCTGGCAGCGCGGCACCTGTGAGAACACCAATGGTCTGCTGCGCCAGTACTTACCCAAGGGCACCGACCTGT
CCGTCTACTCGCAGGAGGAACTTGACGCCATCGCCGACAGCCTCAACGGCCGCCCGCGCAAAACCCTGAACTGGCACTCC
CCGCTACAAGTCCTCGCTCAGGTTCTGGCCAATCCCACGGACCGGCTTCCTGTTCAATAA

Upstream 100 bases:

>100_bases
TTGGCACTGTTTCTGAAGCTACCGCCGACCGTATGCCAGTTGGATCGGGGCGGCCTCAAATCTGAAGTGCAACACCTTGC
CATTCGGTAAGGTGTGGTGA

Downstream 100 bases:

>100_bases
CCAAGGGGTGTTGCGCTTCGCACTTGAAACCGCCCGGTGATTTACACCGTGTCTGGCGACTGGGATGCTTACGGCGGTCA
TAATGCGGGCTGGTGCCAAA

Product: transposase for insertion sequence element IS1086

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MTRTKYQQLQPEERMRIEIWKAEDVSLRAMARRLGRAPSTLMRELRRNATARGGYGAMSAQACRTQRLKASRPVAKLAPD
GVLWGVVRHFLDQKWSPQEISATLKRAFPDQPDLNVSHETIYNAIYAYPRGELRRQLIACLRQARTKRLPRSRGTDRRGQ
IPDMVSIHVRPPEVNDRLMPGHWEGDLIKGAGNQSAVGVLVERMSRAVLLVKMPDATAASALAGFTGKLQSLVAPLRQTL
TYDQGREMARHAELSAATGVRVYFCDPHSPWQRGTCENTNGLLRQYLPKGTDLSVYSQEELDAIADSLNGRPRKTLNWHS
PLQVLAQVLANPTDRLPVQ

Sequences:

>Translated_339_residues
MTRTKYQQLQPEERMRIEIWKAEDVSLRAMARRLGRAPSTLMRELRRNATARGGYGAMSAQACRTQRLKASRPVAKLAPD
GVLWGVVRHFLDQKWSPQEISATLKRAFPDQPDLNVSHETIYNAIYAYPRGELRRQLIACLRQARTKRLPRSRGTDRRGQ
IPDMVSIHVRPPEVNDRLMPGHWEGDLIKGAGNQSAVGVLVERMSRAVLLVKMPDATAASALAGFTGKLQSLVAPLRQTL
TYDQGREMARHAELSAATGVRVYFCDPHSPWQRGTCENTNGLLRQYLPKGTDLSVYSQEELDAIADSLNGRPRKTLNWHS
PLQVLAQVLANPTDRLPVQ
>Mature_338_residues
TRTKYQQLQPEERMRIEIWKAEDVSLRAMARRLGRAPSTLMRELRRNATARGGYGAMSAQACRTQRLKASRPVAKLAPDG
VLWGVVRHFLDQKWSPQEISATLKRAFPDQPDLNVSHETIYNAIYAYPRGELRRQLIACLRQARTKRLPRSRGTDRRGQI
PDMVSIHVRPPEVNDRLMPGHWEGDLIKGAGNQSAVGVLVERMSRAVLLVKMPDATAASALAGFTGKLQSLVAPLRQTLT
YDQGREMARHAELSAATGVRVYFCDPHSPWQRGTCENTNGLLRQYLPKGTDLSVYSQEELDAIADSLNGRPRKTLNWHSP
LQVLAQVLANPTDRLPVQ

Specific function: Required for the transposition of the insertion element (Potential)

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain

Homologues:

Organism=Escherichia coli, GI1786450, Length=329, Percent_Identity=42.2492401215805, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI1790736, Length=329, Percent_Identity=41.9452887537994, Blast_Score=230, Evalue=9e-62,
Organism=Escherichia coli, GI1787671, Length=329, Percent_Identity=41.9452887537994, Blast_Score=230, Evalue=9e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRA8_RALME (P37248)

Other databases:

- EMBL:   X58441
- EMBL:   CP000352
- EMBL:   CP000353
- EMBL:   X90708
- EMBL:   CP000355
- RefSeq:   YP_161657.1
- RefSeq:   YP_582091.1
- RefSeq:   YP_584984.1
- RefSeq:   YP_586141.1
- ProteinModelPortal:   P37248
- GeneID:   3187335
- GeneID:   4036720
- GeneID:   4039670
- GeneID:   4040862
- GenomeReviews:   CP000352_GR
- GenomeReviews:   CP000353_GR
- GenomeReviews:   CP000355_GR
- KEGG:   rme:Rmet_2842
- KEGG:   rme:Rmet_4004
- KEGG:   rme:Rmet_6282
- eggNOG:   COG2826
- HOGENOM:   HBG755150
- OMA:   RSACTLL
- ProtClustDB:   CLSK933005
- BioCyc:   RMET266264:RMET_2842-MONOMER
- BioCyc:   RMET266264:RMET_4004-MONOMER
- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598

Pfam domain/function: PF00665 rve; SSF53098 RNaseH_fold

EC number: NA

Molecular weight: Translated: 38101; Mature: 37970

Theoretical pI: Translated: 10.78; Mature: 10.78

Prosite motif: PS50994 INTEGRASE; PS01043 TRANSPOSASE_IS30

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTKYQQLQPEERMRIEIWKAEDVSLRAMARRLGRAPSTLMRELRRNATARGGYGAMSA
CCCCHHHHCCCCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHH
QACRTQRLKASRPVAKLAPDGVLWGVVRHFLDQKWSPQEISATLKRAFPDQPDLNVSHET
HHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHH
IYNAIYAYPRGELRRQLIACLRQARTKRLPRSRGTDRRGQIPDMVSIHVRPPEVNDRLMP
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCC
GHWEGDLIKGAGNQSAVGVLVERMSRAVLLVKMPDATAASALAGFTGKLQSLVAPLRQTL
CCCCCCEEECCCCCHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
TYDQGREMARHAELSAATGVRVYFCDPHSPWQRGTCENTNGLLRQYLPKGTDLSVYSQEE
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECHHH
LDAIADSLNGRPRKTLNWHSPLQVLAQVLANPTDRLPVQ
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TRTKYQQLQPEERMRIEIWKAEDVSLRAMARRLGRAPSTLMRELRRNATARGGYGAMSA
CCCHHHHCCCCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHH
QACRTQRLKASRPVAKLAPDGVLWGVVRHFLDQKWSPQEISATLKRAFPDQPDLNVSHET
HHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHH
IYNAIYAYPRGELRRQLIACLRQARTKRLPRSRGTDRRGQIPDMVSIHVRPPEVNDRLMP
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCC
GHWEGDLIKGAGNQSAVGVLVERMSRAVLLVKMPDATAASALAGFTGKLQSLVAPLRQTL
CCCCCCEEECCCCCHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
TYDQGREMARHAELSAATGVRVYFCDPHSPWQRGTCENTNGLLRQYLPKGTDLSVYSQEE
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECHHH
LDAIADSLNGRPRKTLNWHSPLQVLAQVLANPTDRLPVQ
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1334071