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The map label for this gene is phaZ3 [H]

Identifier: 94312877

GI number: 94312877

Start: 505863

End: 506936

Strand: Direct

Name: phaZ3 [H]

Synonym: Rmet_3949

Alternate gene names: 94312877

Gene position: 505863-506936 (Clockwise)

Preceding gene: 94312875

Following gene: 94312879

Centisome position: 19.61

GC content: 66.39

Gene sequence:

>1074_bases
ATGGCCAAAAGCCTCTCTCCGGACTGGGATGCGCAGCTTCGACAAATTCGTCGCGCGCAGTTTCAGCCCGCGAAACAAAT
TCGTACATGGCTGGAGCGTCTGGAACTGGCGCCCCCAGCCACTCATCGCCAGCGCCACACCGCGCCAGGCGAGGCCGGGC
CGGCTGAATTGCCAGGCAACTGGCAGTCGCTGAATCTGGGCCTGACGCCGTTACCCGGCGAACTGGTCCCGCAACTCTCG
TACCGCCTCTATATCCCGTCCGGCGCCAAGGGCGCATTGCCTGTCGTGGTGATGCTGCACGGATGTCAGCAAACGCCTGA
AGCCCTGGCGGCTGGCACGCGCATGAACGCGCTGGCCGAGCGCGAAGGATTCATGGTGGCTTATCCGCAGCAGCCATTGC
GGCGCTCCGTGCATCGGTGCTGGCAGTGGTTCGACCTCGCGGCCGGGCAGGGCGGGCGCGAAGCACAGGCGCTGGCACTG
CTGATCGACGAACTGGCAACCCGGCCCGACGTACGTATCGGCGAGATCTACCTTGCCGGGTTGTCGGCCGGTGCCGCGAT
GGCGGCTCTGGTGGCGTTGCGCTACCCGGACAAGGTCGCTGCCGTGGGATTGCACTCCGGTGTGGTGATCGGTGCGGCCG
ATTCGCCCCGGGCGGGCCTGAGGGCGATGCACGAGGGCTCGGCGATCGAGCCCGCGCTATTGCTCGATGCTGCTGGCGTG
ACGCCCGGCGGTCCGGCCATGCCGGCCATCGTGTTGCACGGCATGGCGGACGAAGCGGTGCATCCGATCAACGGGCGTTT
GTTGACGCAGCAGTTCCTGGCCTACAACGGCCTGGGTGGCAGCCTCGGCGGACAGATCGAAAGCGCTAACGATGCGCCGC
TGGGCAAGGATGGCGAAGTTCGCGAAGCGCGCTTTGGCCGCTGGAATCGCGATCTGGTCCGACTGGTGGAAGTGGGGGGG
CTGGGTCATGCCTGGAGTGGCGGCGATGCCACGGTACCGTACCACAGCAGCATCGGCCCGGATGCGAGCTGGCTGATATG
GCAATTTCTCCGTCAGCACAAGCGGGCGGCGTGA

Upstream 100 bases:

>100_bases
GCCTTCAAGACAATGTCGGAAAAGCGCCTCGTCGATTGTGTTATGCTGCATCGCACAAGAAAGGAGGAGACCCGTTCTCA
AGACGAACAGGAGTCTTCAT

Downstream 100 bases:

>100_bases
CAACGCAGGACGGGGCCCGAAGCGCGCCCAAATGAAAAAGGGGGAAGGCTGTCAGCCTTCCCCCTTTTCGATTGTGCCAG
CGCGGCAAGCGTGGCGAGCG

Product: poly(3-hydroxybutyrate) (PHB) depolymerase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MAKSLSPDWDAQLRQIRRAQFQPAKQIRTWLERLELAPPATHRQRHTAPGEAGPAELPGNWQSLNLGLTPLPGELVPQLS
YRLYIPSGAKGALPVVVMLHGCQQTPEALAAGTRMNALAEREGFMVAYPQQPLRRSVHRCWQWFDLAAGQGGREAQALAL
LIDELATRPDVRIGEIYLAGLSAGAAMAALVALRYPDKVAAVGLHSGVVIGAADSPRAGLRAMHEGSAIEPALLLDAAGV
TPGGPAMPAIVLHGMADEAVHPINGRLLTQQFLAYNGLGGSLGGQIESANDAPLGKDGEVREARFGRWNRDLVRLVEVGG
LGHAWSGGDATVPYHSSIGPDASWLIWQFLRQHKRAA

Sequences:

>Translated_357_residues
MAKSLSPDWDAQLRQIRRAQFQPAKQIRTWLERLELAPPATHRQRHTAPGEAGPAELPGNWQSLNLGLTPLPGELVPQLS
YRLYIPSGAKGALPVVVMLHGCQQTPEALAAGTRMNALAEREGFMVAYPQQPLRRSVHRCWQWFDLAAGQGGREAQALAL
LIDELATRPDVRIGEIYLAGLSAGAAMAALVALRYPDKVAAVGLHSGVVIGAADSPRAGLRAMHEGSAIEPALLLDAAGV
TPGGPAMPAIVLHGMADEAVHPINGRLLTQQFLAYNGLGGSLGGQIESANDAPLGKDGEVREARFGRWNRDLVRLVEVGG
LGHAWSGGDATVPYHSSIGPDASWLIWQFLRQHKRAA
>Mature_356_residues
AKSLSPDWDAQLRQIRRAQFQPAKQIRTWLERLELAPPATHRQRHTAPGEAGPAELPGNWQSLNLGLTPLPGELVPQLSY
RLYIPSGAKGALPVVVMLHGCQQTPEALAAGTRMNALAEREGFMVAYPQQPLRRSVHRCWQWFDLAAGQGGREAQALALL
IDELATRPDVRIGEIYLAGLSAGAAMAALVALRYPDKVAAVGLHSGVVIGAADSPRAGLRAMHEGSAIEPALLLDAAGVT
PGGPAMPAIVLHGMADEAVHPINGRLLTQQFLAYNGLGGSLGGQIESANDAPLGKDGEVREARFGRWNRDLVRLVEVGGL
GHAWSGGDATVPYHSSIGPDASWLIWQFLRQHKRAA

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 38178; Mature: 38047

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSLSPDWDAQLRQIRRAQFQPAKQIRTWLERLELAPPATHRQRHTAPGEAGPAELPGN
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
WQSLNLGLTPLPGELVPQLSYRLYIPSGAKGALPVVVMLHGCQQTPEALAAGTRMNALAE
CEEEECCCCCCCHHHHCCCEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHCCHHHHHHH
REGFMVAYPQQPLRRSVHRCWQWFDLAAGQGGREAQALALLIDELATRPDVRIGEIYLAG
CCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEC
LSAGAAMAALVALRYPDKVAAVGLHSGVVIGAADSPRAGLRAMHEGSAIEPALLLDAAGV
CCHHHHHHHHHHHCCCCHHHEEECCCCEEEECCCCCHHHHHHHHCCCCCCHHHEEEECCC
TPGGPAMPAIVLHGMADEAVHPINGRLLTQQFLAYNGLGGSLGGQIESANDAPLGKDGEV
CCCCCCCHHHHHHCCHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCH
REARFGRWNRDLVRLVEVGGLGHAWSGGDATVPYHSSIGPDASWLIWQFLRQHKRAA
HHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKSLSPDWDAQLRQIRRAQFQPAKQIRTWLERLELAPPATHRQRHTAPGEAGPAELPGN
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
WQSLNLGLTPLPGELVPQLSYRLYIPSGAKGALPVVVMLHGCQQTPEALAAGTRMNALAE
CEEEECCCCCCCHHHHCCCEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHCCHHHHHHH
REGFMVAYPQQPLRRSVHRCWQWFDLAAGQGGREAQALALLIDELATRPDVRIGEIYLAG
CCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEC
LSAGAAMAALVALRYPDKVAAVGLHSGVVIGAADSPRAGLRAMHEGSAIEPALLLDAAGV
CCHHHHHHHHHHHCCCCHHHEEECCCCEEEECCCCCHHHHHHHHCCCCCCHHHEEEECCC
TPGGPAMPAIVLHGMADEAVHPINGRLLTQQFLAYNGLGGSLGGQIESANDAPLGKDGEV
CCCCCCCHHHHHHCCHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCH
REARFGRWNRDLVRLVEVGGLGHAWSGGDATVPYHSSIGPDASWLIWQFLRQHKRAA
HHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]