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The map label for this gene is cobB [H]

Identifier: 94312866

GI number: 94312866

Start: 496205

End: 497626

Strand: Reverse

Name: cobB [H]

Synonym: Rmet_3938

Alternate gene names: 94312866

Gene position: 497626-496205 (Counterclockwise)

Preceding gene: 94312867

Following gene: 94312861

Centisome position: 19.29

GC content: 65.75

Gene sequence:

>1422_bases
ATGTCTGCCCGATATAATCCATCGCATCGCATGGCGCTGCCGCCGTGCATTGCCACCTCCGAGATGCCCCCGCTTCCTAT
TCAATCCTCTCCGTTGGGCACGACAACACGAACCGGCACGCGCCCGGTCCCCGCGTTGCTCGTTGCCGCCCCCGCATCGG
GGCAGGGCAAGACCACGGTCACCGCCGCCTTGGCACGCCTTCATGCGCGGCAGGGCCGTACCGTGCGCGTATTCAAGATC
GGGCCCGATTTCCTCGACCCGACGTTGCTTGCCGCCGCCAGCGGTGCTCCGGTGCAATCGATCGATCTCTGGATGACAGG
GGAAGTCGATGCTCGCCAACGTCTTGCCCTGGCCGCGCAGACCGCGGATCTGATTCTTGTCGAAGGTGTCATGGGCCTGC
ACGATGGCACGCCGAGCAGTGCGGATGTCGCGCGATGCTTTAGACTGCCGGTCCTCGCCGTCATCCAGGCAGGCGCGATG
GCGCAAACGTTCGGTGCCGTGGCCCACGGCCTGGCGACCTACGGCGAGCCATTTCACGCGATGCACGTGCTGGCGAATGG
CGTCGGCAGCGCACGCCATGCCGAGATGCTGCGAGACAGCCTGCCGCATGACATCCACTGGGCCGGCGCGTTGGCCCGCG
ATGATGCGGCGGCGTTGCCGGAGCGGCATCTTGGCCTGTTCAGCGCCGACGAGCTTCCCGACCTTACCGCGCGACTGGAT
CGCATGGCGGATGCCTTGGCCGCACTGCCGCTATCGCACCTGCCAGAGCCGATCGTGTTTCCGGACACGCCTACGCAGCA
ATTGCCGCGCCTGCTGGAGGGCCGGCGCATCGCCATCGCGCGCGACGATGCCTTCCGGTTCATTTATCCGGCCAATGTGG
AAACGTTGAACGCGCTCGGGGCGCATATCACATGGTTCTCGCCCTTGCGTGACGCGGCTTTGCCGCACTGTGACGCGCTA
TGGCTGCCCGGTGGCTACCCCGAACTGCACGCCGCCGCGCTCGCCGCGAATGTGCCGATGCACAACGCGATCCGCGCGGC
GCACGCACACGGCCTGCCAATGCTGGCGGAGTGCGGCGGCATGATGGCGCTGTTCGACAACCTGGTGGACAAGGAAGGGC
AAACCCATCGCATGGCCGGGCTGCTGCCCGGCACGGTGACGATGCAAAAACGACTGGCGGCAATCGGCCATCAGGCCATC
GAGCTACCTACGAGGGAAGGCATGGTCGGCCAGCCGGTACGCGGACACACGTTTCACTACTCGACCACCGACACTCCGCT
GACTCCGCTCCTGCACGCGCGGTCACCGCGCGATGATGGAACCGGGGAAGCTGTCTATCGGTCCGGGTCGCTTACCGCGT
CCTACGTGCACCTGTACTTCCCTTCTAACCCAACGGCCATAGCGGCGTTGTTTGGCGGATAG

Upstream 100 bases:

>100_bases
AGAACTGCGCCATGCAGAAGCTCGGCGTCACCAATGACCGCGAGCTGTTCGAGTGTGTGGCACTTCAGGGCATGGTCCTG
CCACGGCGCTAAAGGTGGAG

Downstream 100 bases:

>100_bases
AGAGCAGCCAGGCGACGCTGCCAGATCAGGCGGCGTCGCGTTCCGCCTGCGTGCGCGCGACTGCGTCGGCGATGCCCGCC
TCGAGCTGAACCAGCCCCTT

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSARYNPSHRMALPPCIATSEMPPLPIQSSPLGTTTRTGTRPVPALLVAAPASGQGKTTVTAALARLHARQGRTVRVFKI
GPDFLDPTLLAAASGAPVQSIDLWMTGEVDARQRLALAAQTADLILVEGVMGLHDGTPSSADVARCFRLPVLAVIQAGAM
AQTFGAVAHGLATYGEPFHAMHVLANGVGSARHAEMLRDSLPHDIHWAGALARDDAAALPERHLGLFSADELPDLTARLD
RMADALAALPLSHLPEPIVFPDTPTQQLPRLLEGRRIAIARDDAFRFIYPANVETLNALGAHITWFSPLRDAALPHCDAL
WLPGGYPELHAAALAANVPMHNAIRAAHAHGLPMLAECGGMMALFDNLVDKEGQTHRMAGLLPGTVTMQKRLAAIGHQAI
ELPTREGMVGQPVRGHTFHYSTTDTPLTPLLHARSPRDDGTGEAVYRSGSLTASYVHLYFPSNPTAIAALFGG

Sequences:

>Translated_473_residues
MSARYNPSHRMALPPCIATSEMPPLPIQSSPLGTTTRTGTRPVPALLVAAPASGQGKTTVTAALARLHARQGRTVRVFKI
GPDFLDPTLLAAASGAPVQSIDLWMTGEVDARQRLALAAQTADLILVEGVMGLHDGTPSSADVARCFRLPVLAVIQAGAM
AQTFGAVAHGLATYGEPFHAMHVLANGVGSARHAEMLRDSLPHDIHWAGALARDDAAALPERHLGLFSADELPDLTARLD
RMADALAALPLSHLPEPIVFPDTPTQQLPRLLEGRRIAIARDDAFRFIYPANVETLNALGAHITWFSPLRDAALPHCDAL
WLPGGYPELHAAALAANVPMHNAIRAAHAHGLPMLAECGGMMALFDNLVDKEGQTHRMAGLLPGTVTMQKRLAAIGHQAI
ELPTREGMVGQPVRGHTFHYSTTDTPLTPLLHARSPRDDGTGEAVYRSGSLTASYVHLYFPSNPTAIAALFGG
>Mature_472_residues
SARYNPSHRMALPPCIATSEMPPLPIQSSPLGTTTRTGTRPVPALLVAAPASGQGKTTVTAALARLHARQGRTVRVFKIG
PDFLDPTLLAAASGAPVQSIDLWMTGEVDARQRLALAAQTADLILVEGVMGLHDGTPSSADVARCFRLPVLAVIQAGAMA
QTFGAVAHGLATYGEPFHAMHVLANGVGSARHAEMLRDSLPHDIHWAGALARDDAAALPERHLGLFSADELPDLTARLDR
MADALAALPLSHLPEPIVFPDTPTQQLPRLLEGRRIAIARDDAFRFIYPANVETLNALGAHITWFSPLRDAALPHCDALW
LPGGYPELHAAALAANVPMHNAIRAAHAHGLPMLAECGGMMALFDNLVDKEGQTHRMAGLLPGTVTMQKRLAAIGHQAIE
LPTREGMVGQPVRGHTFHYSTTDTPLTPLLHARSPRDDGTGEAVYRSGSLTASYVHLYFPSNPTAIAALFGG

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 50257; Mature: 50126

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARYNPSHRMALPPCIATSEMPPLPIQSSPLGTTTRTGTRPVPALLVAAPASGQGKTTV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHH
TAALARLHARQGRTVRVFKIGPDFLDPTLLAAASGAPVQSIDLWMTGEVDARQRLALAAQ
HHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHH
TADLILVEGVMGLHDGTPSSADVARCFRLPVLAVIQAGAMAQTFGAVAHGLATYGEPFHA
HHCEEEEECHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHH
MHVLANGVGSARHAEMLRDSLPHDIHWAGALARDDAAALPERHLGLFSADELPDLTARLD
HHHHHHCCCCHHHHHHHHHCCCCCEECCCCCCCCCHHHCCHHHCCCCCCCCCCHHHHHHH
RMADALAALPLSHLPEPIVFPDTPTQQLPRLLEGRRIAIARDDAFRFIYPANVETLNALG
HHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCEEEEECCCCHHHHHCC
AHITWFSPLRDAALPHCDALWLPGGYPELHAAALAANVPMHNAIRAAHAHGLPMLAECGG
CCEEEECHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHCC
MMALFDNLVDKEGQTHRMAGLLPGTVTMQKRLAAIGHQAIELPTREGMVGQPVRGHTFHY
HHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHCHHHEECCCCCCCCCCCCCCCEEEE
STTDTPLTPLLHARSPRDDGTGEAVYRSGSLTASYVHLYFPSNPTAIAALFGG
CCCCCCCCHHHHCCCCCCCCCCCEEEECCCCEEEEEEEEECCCCCEEEEEECC
>Mature Secondary Structure 
SARYNPSHRMALPPCIATSEMPPLPIQSSPLGTTTRTGTRPVPALLVAAPASGQGKTTV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHH
TAALARLHARQGRTVRVFKIGPDFLDPTLLAAASGAPVQSIDLWMTGEVDARQRLALAAQ
HHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHH
TADLILVEGVMGLHDGTPSSADVARCFRLPVLAVIQAGAMAQTFGAVAHGLATYGEPFHA
HHCEEEEECHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHH
MHVLANGVGSARHAEMLRDSLPHDIHWAGALARDDAAALPERHLGLFSADELPDLTARLD
HHHHHHCCCCHHHHHHHHHCCCCCEECCCCCCCCCHHHCCHHHCCCCCCCCCCHHHHHHH
RMADALAALPLSHLPEPIVFPDTPTQQLPRLLEGRRIAIARDDAFRFIYPANVETLNALG
HHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCEEEEECCCCHHHHHCC
AHITWFSPLRDAALPHCDALWLPGGYPELHAAALAANVPMHNAIRAAHAHGLPMLAECGG
CCEEEECHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHCC
MMALFDNLVDKEGQTHRMAGLLPGTVTMQKRLAAIGHQAIELPTREGMVGQPVRGHTFHY
HHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHCHHHEECCCCCCCCCCCCCCCEEEE
STTDTPLTPLLHARSPRDDGTGEAVYRSGSLTASYVHLYFPSNPTAIAALFGG
CCCCCCCCHHHHCCCCCCCCCCCEEEECCCCEEEEEEEEECCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA