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The map label for this gene is cysL [H]

Identifier: 94312829

GI number: 94312829

Start: 446493

End: 447395

Strand: Reverse

Name: cysL [H]

Synonym: Rmet_3901

Alternate gene names: 94312829

Gene position: 447395-446493 (Counterclockwise)

Preceding gene: 94312831

Following gene: 94312827

Centisome position: 17.34

GC content: 65.67

Gene sequence:

>903_bases
ATGCACTACGACCTGACCGACCTGCGGCTGTTTCTGAACGTGGGAGAAACAGAGAACATCACACGCGCGGCCGAGCGCAG
CTTTCTCTCGCTGCCCGCGGCCAGCGCCCGCATCAAGCAGCTTGAAGAGGCGTTCCAGACCCAGTTGCTGATCCGCCAGG
TGAAAGGCGTGCGCCTCACTCCGGCTGGCGACGCCCTGCTGCGCCACGCGCGCGAGGTGTTCCGGGAGCTCGAATGCCTG
CATTCGGACCTGCGCCCCTATGCCAAGGGCGTCAAGGGCCGCGTGCGCCTCCTGGCCAACACCACGGCCACCAACTCGTT
CCTGGCCCACGCGCTGTCGCGCTTCCTGTCGGAGAACCCGGACGTCGACATCGAGTTGGAGGAACACCTTTCGGCCGACA
TCGTCTCGGCCATCGGCACGGGGGCGGCGGACCTCGGCATCGTGGCGGGAGAAGTCGCCACCGCCGACCTGGACACAATG
CAGCTTTGCAGCGACGAACTGATCGTGATCGCCCCTGTCAACCATCCGCTGCCAGCCTTCAAGACACTGCGGTTCGCCGA
ACTGCTCGATAGTTGCCGCTTTGTGGGGCTGAATCAGTCATCGGCGATCCAGTCCTTCCTGGACCGCATCGCCAGCGGCA
TGGGCAAACGGATCAGCCTGCGCATCCAGGTGGGCAGCTTCGACGCGGTGTGCCGCATGGTGGAAGCCGGCGCCGGCATC
GCCATCGTGCCGAACAGTTGCGCGCGGCGCTACGCCAGCCGCAAGGTACTGCGTTTCATCCGGCTGGAAGACGACTGGGC
GCGACGCGAGTTGCAACTCGTGCGCCGGCCGGGACGGGAACTGCCCCAGTTCGCGGAAACGCTGATTCAGTATCTGGTGG
ATGCGGCCCGGGAGCCGGTGTAG

Upstream 100 bases:

>100_bases
GAAGCCTGCCCGTTTTATTGCAGAGCTGGATACGATAAATTGGCGGCCGAAACTTTGGAATTCATGAATCACAAAAGTGG
GCTTCGTGCCCGCCGAAGCC

Downstream 100 bases:

>100_bases
GTGTTTCCCCCCTCAATCCGCCTTAGTCCGCCTTCACGCCAGCCGCCTTGATAATCGGCGACCACTTGTCGATCTCCGAG
GCCAGCCACGTGCGCAGCCC

Product: transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MHYDLTDLRLFLNVGETENITRAAERSFLSLPAASARIKQLEEAFQTQLLIRQVKGVRLTPAGDALLRHAREVFRELECL
HSDLRPYAKGVKGRVRLLANTTATNSFLAHALSRFLSENPDVDIELEEHLSADIVSAIGTGAADLGIVAGEVATADLDTM
QLCSDELIVIAPVNHPLPAFKTLRFAELLDSCRFVGLNQSSAIQSFLDRIASGMGKRISLRIQVGSFDAVCRMVEAGAGI
AIVPNSCARRYASRKVLRFIRLEDDWARRELQLVRRPGRELPQFAETLIQYLVDAAREPV

Sequences:

>Translated_300_residues
MHYDLTDLRLFLNVGETENITRAAERSFLSLPAASARIKQLEEAFQTQLLIRQVKGVRLTPAGDALLRHAREVFRELECL
HSDLRPYAKGVKGRVRLLANTTATNSFLAHALSRFLSENPDVDIELEEHLSADIVSAIGTGAADLGIVAGEVATADLDTM
QLCSDELIVIAPVNHPLPAFKTLRFAELLDSCRFVGLNQSSAIQSFLDRIASGMGKRISLRIQVGSFDAVCRMVEAGAGI
AIVPNSCARRYASRKVLRFIRLEDDWARRELQLVRRPGRELPQFAETLIQYLVDAAREPV
>Mature_300_residues
MHYDLTDLRLFLNVGETENITRAAERSFLSLPAASARIKQLEEAFQTQLLIRQVKGVRLTPAGDALLRHAREVFRELECL
HSDLRPYAKGVKGRVRLLANTTATNSFLAHALSRFLSENPDVDIELEEHLSADIVSAIGTGAADLGIVAGEVATADLDTM
QLCSDELIVIAPVNHPLPAFKTLRFAELLDSCRFVGLNQSSAIQSFLDRIASGMGKRISLRIQVGSFDAVCRMVEAGAGI
AIVPNSCARRYASRKVLRFIRLEDDWARRELQLVRRPGRELPQFAETLIQYLVDAAREPV

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693105, Length=294, Percent_Identity=24.8299319727891, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI2367136, Length=248, Percent_Identity=31.0483870967742, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI157672245, Length=269, Percent_Identity=25.2788104089219, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788887, Length=196, Percent_Identity=31.6326530612245, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787879, Length=304, Percent_Identity=25.3289473684211, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788748, Length=294, Percent_Identity=26.1904761904762, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790208, Length=253, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787601, Length=188, Percent_Identity=25, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788297, Length=246, Percent_Identity=24.7967479674797, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787806, Length=299, Percent_Identity=22.0735785953177, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786713, Length=249, Percent_Identity=28.1124497991968, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI145693193, Length=124, Percent_Identity=33.8709677419355, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790399, Length=278, Percent_Identity=21.9424460431655, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787530, Length=247, Percent_Identity=24.6963562753036, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788481, Length=251, Percent_Identity=25.4980079681275, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33248; Mature: 33248

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYDLTDLRLFLNVGETENITRAAERSFLSLPAASARIKQLEEAFQTQLLIRQVKGVRLT
CCCCHHHHHHEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PAGDALLRHAREVFRELECLHSDLRPYAKGVKGRVRLLANTTATNSFLAHALSRFLSENP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEECCCHHHHHHHHHHHHHHCCCC
DVDIELEEHLSADIVSAIGTGAADLGIVAGEVATADLDTMQLCSDELIVIAPVNHPLPAF
CCEEEHHHHHHHHHHHHHCCCCHHHHHHCCHHHHCCHHHHHHCCCCEEEEEECCCCCHHH
KTLRFAELLDSCRFVGLNQSSAIQSFLDRIASGMGKRISLRIQVGSFDAVCRMVEAGAGI
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHCCCCE
AIVPNSCARRYASRKVLRFIRLEDDWARRELQLVRRPGRELPQFAETLIQYLVDAAREPV
EECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHYDLTDLRLFLNVGETENITRAAERSFLSLPAASARIKQLEEAFQTQLLIRQVKGVRLT
CCCCHHHHHHEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PAGDALLRHAREVFRELECLHSDLRPYAKGVKGRVRLLANTTATNSFLAHALSRFLSENP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEECCCHHHHHHHHHHHHHHCCCC
DVDIELEEHLSADIVSAIGTGAADLGIVAGEVATADLDTMQLCSDELIVIAPVNHPLPAF
CCEEEHHHHHHHHHHHHHCCCCHHHHHHCCHHHHCCHHHHHHCCCCEEEEEECCCCCHHH
KTLRFAELLDSCRFVGLNQSSAIQSFLDRIASGMGKRISLRIQVGSFDAVCRMVEAGAGI
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHCCCCE
AIVPNSCARRYASRKVLRFIRLEDDWARRELQLVRRPGRELPQFAETLIQYLVDAAREPV
EECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]