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The map label for this gene is nemA [H]

Identifier: 94312797

GI number: 94312797

Start: 412577

End: 413656

Strand: Reverse

Name: nemA [H]

Synonym: Rmet_3869

Alternate gene names: 94312797

Gene position: 413656-412577 (Counterclockwise)

Preceding gene: 94312799

Following gene: 94312795

Centisome position: 16.03

GC content: 66.02

Gene sequence:

>1080_bases
ATGAGCGCACTGTTCACCCCGGTCCGCGTCGGCCGATACACCCTGAAGAATCATCTGGTCATGGCGCCGATGACCCGTAG
CCGCGCCAGCCTTGATGGCACGCCGGGCGAACTGGCAGCCGAGTACTACGCGCAGCGCGCGAGTGTCGGCCTGATCGTGA
CCGAAGGGACCCAGCCTTCGGACGACGGGCAGGGGTACCTCGCCACGCCCGGTATCTACACCGAAGCCCATGTCGATGGA
TGGAAGAACGTCACCGCCGCAGTACACGAGCGAGGCGGTCGGATCTTCATCCAGCTCATGCACGCGGGACGCATGTCCCA
CCCGGACAACACGCCGCATCACCGGCAAGGTGTGGCGCCGTCGGCCATTGCCCCCGGTGTGCCGATGTTCACGCTGAAGG
GTATGCAGGACATTCCGGCACCGCGCGCGCTGACCACTGCCGAGGTACGCCAGACCGTGGACGACTTCCGCTTCGCCGCC
CGGCAAGCGATCGCCGCAGGCGCGGACGGCGTCGAGATCCACGGGGCCAATGGTTATCTGGTCCAGCAATTCTTCGCGCC
CAACGCTAATACCCGCACCGACAAATACGGCGGCTCGATTGACAACCGCGCCCGCTTCGCCATCGAGGTGGCCACCGCAA
TCGCCGGGGAAATCGGCGCGGACCGGACGGCCATCAGACTATCGCCCGGCACGCCGCTGTGGGGCATCGACGAAGGCGCC
GAAGGTCCGGCGCTCTATCGCCACCTGGTAACGGAACTAGACAAGCTCGGCCTGGCGTACCTGCACGTCAGCCTCCAGGG
CAACGAGCCACAGTTGGCCGACATCCGCGAACGCTGGCGCGGCACGCTGATCCTGAACCGGCCGGGCCGCACCCGTGAAG
CTGTTGGTGCCGACGTGGCATCCGGGCTTGCCGATCTGGAAGCCTATGGACAGATGGTGCTGGCCAACCCGGACTTCGTC
ACCCGCCTGAAGCTCGGCGCGCCGATGAACGATGCCCTTCCCAATGCGTTCTTTGGTGGCGACGGGCGTGGGTATATCGA
CTACCCGGCCCTGGCCCTGACCGATACCGTGACGTCCTGA

Upstream 100 bases:

>100_bases
GGCGCCATGGAAGCCTGATCCGCGCGATGGCGCGCGTCAGCGCCTGACCGGCATCACGAACTGATGCCGGCAACCTGCAC
TTTCGAAAGAGGTCATGCAC

Downstream 100 bases:

>100_bases
GGCGCTGGCACGTGCCGCCTCGCCGTCGCTGAGACTGGCGGGGCGGCAATCCCGGCCAATTCGCTATCAACTCGCCCTCA
AATCGGCGTCACGGCAAGCC

Product: putative oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MSALFTPVRVGRYTLKNHLVMAPMTRSRASLDGTPGELAAEYYAQRASVGLIVTEGTQPSDDGQGYLATPGIYTEAHVDG
WKNVTAAVHERGGRIFIQLMHAGRMSHPDNTPHHRQGVAPSAIAPGVPMFTLKGMQDIPAPRALTTAEVRQTVDDFRFAA
RQAIAAGADGVEIHGANGYLVQQFFAPNANTRTDKYGGSIDNRARFAIEVATAIAGEIGADRTAIRLSPGTPLWGIDEGA
EGPALYRHLVTELDKLGLAYLHVSLQGNEPQLADIRERWRGTLILNRPGRTREAVGADVASGLADLEAYGQMVLANPDFV
TRLKLGAPMNDALPNAFFGGDGRGYIDYPALALTDTVTS

Sequences:

>Translated_359_residues
MSALFTPVRVGRYTLKNHLVMAPMTRSRASLDGTPGELAAEYYAQRASVGLIVTEGTQPSDDGQGYLATPGIYTEAHVDG
WKNVTAAVHERGGRIFIQLMHAGRMSHPDNTPHHRQGVAPSAIAPGVPMFTLKGMQDIPAPRALTTAEVRQTVDDFRFAA
RQAIAAGADGVEIHGANGYLVQQFFAPNANTRTDKYGGSIDNRARFAIEVATAIAGEIGADRTAIRLSPGTPLWGIDEGA
EGPALYRHLVTELDKLGLAYLHVSLQGNEPQLADIRERWRGTLILNRPGRTREAVGADVASGLADLEAYGQMVLANPDFV
TRLKLGAPMNDALPNAFFGGDGRGYIDYPALALTDTVTS
>Mature_358_residues
SALFTPVRVGRYTLKNHLVMAPMTRSRASLDGTPGELAAEYYAQRASVGLIVTEGTQPSDDGQGYLATPGIYTEAHVDGW
KNVTAAVHERGGRIFIQLMHAGRMSHPDNTPHHRQGVAPSAIAPGVPMFTLKGMQDIPAPRALTTAEVRQTVDDFRFAAR
QAIAAGADGVEIHGANGYLVQQFFAPNANTRTDKYGGSIDNRARFAIEVATAIAGEIGADRTAIRLSPGTPLWGIDEGAE
GPALYRHLVTELDKLGLAYLHVSLQGNEPQLADIRERWRGTLILNRPGRTREAVGADVASGLADLEAYGQMVLANPDFVT
RLKLGAPMNDALPNAFFGGDGRGYIDYPALALTDTVTS

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=364, Percent_Identity=41.2087912087912, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI1789463, Length=231, Percent_Identity=36.3636363636364, Blast_Score=126, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17559804, Length=135, Percent_Identity=42.962962962963, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17540738, Length=221, Percent_Identity=32.579185520362, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=133, Percent_Identity=39.0977443609023, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17564188, Length=136, Percent_Identity=38.9705882352941, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17559802, Length=132, Percent_Identity=40.1515151515151, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17565138, Length=367, Percent_Identity=25.8855585831063, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6321973, Length=378, Percent_Identity=35.7142857142857, Blast_Score=200, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6325086, Length=373, Percent_Identity=35.656836461126, Blast_Score=197, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 38456; Mature: 38325

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALFTPVRVGRYTLKNHLVMAPMTRSRASLDGTPGELAAEYYAQRASVGLIVTEGTQPS
CCCCCCHHHHHHEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCC
DDGQGYLATPGIYTEAHVDGWKNVTAAVHERGGRIFIQLMHAGRMSHPDNTPHHRQGVAP
CCCCEEEECCCCEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCHHCCCCC
SAIAPGVPMFTLKGMQDIPAPRALTTAEVRQTVDDFRFAARQAIAAGADGVEIHGANGYL
HHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEE
VQQFFAPNANTRTDKYGGSIDNRARFAIEVATAIAGEIGADRTAIRLSPGTPLWGIDEGA
EEEEECCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHCCCCCCEEEEECCCCCCCCCCCCC
EGPALYRHLVTELDKLGLAYLHVSLQGNEPQLADIRERWRGTLILNRPGRTREAVGADVA
CCHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHCCCEEEEECCCCCHHHHHHHHH
SGLADLEAYGQMVLANPDFVTRLKLGAPMNDALPNAFFGGDGRGYIDYPALALTDTVTS
HHHHHHHHHCCEEEECCCEEEEEECCCCCCHHCCCCEECCCCCCEECCCCEEEECCCCC
>Mature Secondary Structure 
SALFTPVRVGRYTLKNHLVMAPMTRSRASLDGTPGELAAEYYAQRASVGLIVTEGTQPS
CCCCCHHHHHHEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCC
DDGQGYLATPGIYTEAHVDGWKNVTAAVHERGGRIFIQLMHAGRMSHPDNTPHHRQGVAP
CCCCEEEECCCCEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCHHCCCCC
SAIAPGVPMFTLKGMQDIPAPRALTTAEVRQTVDDFRFAARQAIAAGADGVEIHGANGYL
HHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEE
VQQFFAPNANTRTDKYGGSIDNRARFAIEVATAIAGEIGADRTAIRLSPGTPLWGIDEGA
EEEEECCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHCCCCCCEEEEECCCCCCCCCCCCC
EGPALYRHLVTELDKLGLAYLHVSLQGNEPQLADIRERWRGTLILNRPGRTREAVGADVA
CCHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHCCCEEEEECCCCCHHHHHHHHH
SGLADLEAYGQMVLANPDFVTRLKLGAPMNDALPNAFFGGDGRGYIDYPALALTDTVTS
HHHHHHHHHCCEEEECCCEEEEEECCCCCCHHCCCCEECCCCCCEECCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]