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The map label for this gene is ubiE [H]

Identifier: 94312774

GI number: 94312774

Start: 389404

End: 390222

Strand: Reverse

Name: ubiE [H]

Synonym: Rmet_3846

Alternate gene names: 94312774

Gene position: 390222-389404 (Counterclockwise)

Preceding gene: 94312775

Following gene: 291481496

Centisome position: 15.12

GC content: 65.32

Gene sequence:

>819_bases
ATGACATCGCATGGAACAGACACGGTCTTCGCAGGCTCCGTTCCGGAGTTGTACGACACGCTGATGGTCCCGTTGATCTT
CGAACCATACGCGGCGGACCTCGCCGCGCGTGCCGCAGCACTCGCGCCAACACACGTGCTCGAGGTTGCTGCGGGAACTG
GCGCGCTGACACGGCGGCTGGCGAGTGACCTGCCCGACGGCACGTCGATCGTGGCCACCGACCTCAATCGACCGATGCTG
GATCGCGCCGCCGCCATCGGCACCTCGCGGCCGCTGACGTGGCGGCAGGCCGACGCCATGCAGCTACCCTTCCCCGACGC
AAGTTTCGATCTGGTGATCTGCCAGTTCGGGGCGATGTTCTTTCCCGAGAAGCACAAGGCTTTCGCCGAAGCGCGCCGTG
TGCTGAAGCCCGGGGGCGTGCTGCTTTTCAACGTGTGGGATCGCATCGAGGAAAACGCGTTCGCGCATACGGTAACGCAA
ACGCTGACCGGCATGTTTCCGCACAAGCCGCCTGTGTTTCTCGCACGCACGCCTCATGGTTATCACGACCCGGCGGTCGT
GGCGGCCGATCTGAGGCTCGGAGGCTTTTCCGAAGCCTCCACATGCGCAACCGTCGCCGCCGAAAGCCGTGCCGTGGACG
CCAACACGCCTGCCATCGCCTTCTGCCAGGGAACGCCGCTACGCGGCGAACTGGAGGCATTCGGCCCCGATGCGCTGGTG
AATGCCACGCTCGCCTGTGCGAAGGCGCTGACGAGGGAGTTCGGCCCCGGTCCGGTTAGCGGGAAGATTCAGGCGCACGT
GTTCAGCGCCAGGGCTTGA

Upstream 100 bases:

>100_bases
GTTGAACCTGGCGCTACGCGCCCCGGCTTAGTTCGCTTCAGAAAGCTGGATCGACCATCCCCCGGCACGCAACACCGGGG
GCAGGGACAAGAGGCGAGCC

Downstream 100 bases:

>100_bases
TCGGCGCGCAATTCAGCCCCCCTGCCACGCCGCCCCACCCATTGACCCCACCCATCGACCCACCCGGCAACACGCTCGAT
CCGACGCACTGCGCTTGATA

Product: putative methyltransferase, ubiE/COQ5 family

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MTSHGTDTVFAGSVPELYDTLMVPLIFEPYAADLAARAAALAPTHVLEVAAGTGALTRRLASDLPDGTSIVATDLNRPML
DRAAAIGTSRPLTWRQADAMQLPFPDASFDLVICQFGAMFFPEKHKAFAEARRVLKPGGVLLFNVWDRIEENAFAHTVTQ
TLTGMFPHKPPVFLARTPHGYHDPAVVAADLRLGGFSEASTCATVAAESRAVDANTPAIAFCQGTPLRGELEAFGPDALV
NATLACAKALTREFGPGPVSGKIQAHVFSARA

Sequences:

>Translated_272_residues
MTSHGTDTVFAGSVPELYDTLMVPLIFEPYAADLAARAAALAPTHVLEVAAGTGALTRRLASDLPDGTSIVATDLNRPML
DRAAAIGTSRPLTWRQADAMQLPFPDASFDLVICQFGAMFFPEKHKAFAEARRVLKPGGVLLFNVWDRIEENAFAHTVTQ
TLTGMFPHKPPVFLARTPHGYHDPAVVAADLRLGGFSEASTCATVAAESRAVDANTPAIAFCQGTPLRGELEAFGPDALV
NATLACAKALTREFGPGPVSGKIQAHVFSARA
>Mature_271_residues
TSHGTDTVFAGSVPELYDTLMVPLIFEPYAADLAARAAALAPTHVLEVAAGTGALTRRLASDLPDGTSIVATDLNRPMLD
RAAAIGTSRPLTWRQADAMQLPFPDASFDLVICQFGAMFFPEKHKAFAEARRVLKPGGVLLFNVWDRIEENAFAHTVTQT
LTGMFPHKPPVFLARTPHGYHDPAVVAADLRLGGFSEASTCATVAAESRAVDANTPAIAFCQGTPLRGELEAFGPDALVN
ATLACAKALTREFGPGPVSGKIQAHVFSARA

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Escherichia coli, GI48994983, Length=101, Percent_Identity=38.6138613861386, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28826; Mature: 28695

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSHGTDTVFAGSVPELYDTLMVPLIFEPYAADLAARAAALAPTHVLEVAAGTGALTRRL
CCCCCCCEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHH
ASDLPDGTSIVATDLNRPMLDRAAAIGTSRPLTWRQADAMQLPFPDASFDLVICQFGAMF
HHCCCCCCEEEEECCCCHHHHHHHHCCCCCCCEECCCCCEECCCCCCCHHHHEEHHCCCC
FPEKHKAFAEARRVLKPGGVLLFNVWDRIEENAFAHTVTQTLTGMFPHKPPVFLARTPHG
CCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
YHDPAVVAADLRLGGFSEASTCATVAAESRAVDANTPAIAFCQGTPLRGELEAFGPDALV
CCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHCCCHHHH
NATLACAKALTREFGPGPVSGKIQAHVFSARA
HHHHHHHHHHHHHCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure 
TSHGTDTVFAGSVPELYDTLMVPLIFEPYAADLAARAAALAPTHVLEVAAGTGALTRRL
CCCCCCEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHH
ASDLPDGTSIVATDLNRPMLDRAAAIGTSRPLTWRQADAMQLPFPDASFDLVICQFGAMF
HHCCCCCCEEEEECCCCHHHHHHHHCCCCCCCEECCCCCEECCCCCCCHHHHEEHHCCCC
FPEKHKAFAEARRVLKPGGVLLFNVWDRIEENAFAHTVTQTLTGMFPHKPPVFLARTPHG
CCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
YHDPAVVAADLRLGGFSEASTCATVAAESRAVDANTPAIAFCQGTPLRGELEAFGPDALV
CCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHCCCHHHH
NATLACAKALTREFGPGPVSGKIQAHVFSARA
HHHHHHHHHHHHHCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12219091 [H]