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The map label for this gene is alsR [H]

Identifier: 94312740

GI number: 94312740

Start: 351213

End: 352331

Strand: Reverse

Name: alsR [H]

Synonym: Rmet_3810

Alternate gene names: 94312740

Gene position: 352331-351213 (Counterclockwise)

Preceding gene: 291481495

Following gene: 94312739

Centisome position: 13.66

GC content: 63.36

Gene sequence:

>1119_bases
ATGAGGTTGTCTCCGTCCGGTTCTGTGGTGACCGGTAACGTCTTTGCAATGTCGCCATGCCACGGCGCGAGCGATCACTA
TAGAATCGACAGCGCGGCTCGGGCCAGTCGAAAAGTACTGAGTCCCCTTAGAAGAATTCGAACCGCGATGCCTGAAGCGC
CCCTGGCCCCTCGGCACCGACCGGCGCCGTGGCAACAGGAGACGACCTTGCGATTTGAGTTAACCGACCTTCGGGTCTTC
GTGGCCATTGCCGAGGCGGGAAGCCTGACGATGGGTGCTTCCGACATGCATCTGACTGCGCCCTCCGCCAGCTATCGGCT
GAAGAATCTGGAGCAGACCGTCGGCGTGCCGCTGTTCAATCGCACGCAGAAGGGCATGGTCCTGACGGCGGCCGGCGAAA
CCATGCTGCAGCACGCCAGGACGATTCTGGACAATGTGGAGCAGTTGCAGATCGGCATGCGGCGCCACACCGATGGCATC
GAAGGGCACATTCGCGTTTACGCGAACAGCAGCACCATGTGCAACCTGCCCGTGGCCCTCAGCCGCTTCCTGGCGGCCTA
TCCCAACGTCAATGTCGACCTCGAGGAGCACCTGAGCGCGGACAGCGTTCGCGCGGTGCAGGAGCAACGCGCCGATATCG
GGCTGGTGGCAGGCGCCATCGACCTGCATGGCCTCGACTCCATCCTCTATGGCGCAGACGAGCTACTGTTCGTGGTGCCG
CCACGTCATCCGCTGGGCATGCGCGATAGCGTCTCGCTGGTCCAGGCGTTGCGCCACGAACTCGTATCGATCGGCAGCAA
GAGCAGCAATTTCCTCTATCTGCAGGATATGGCAACGCGCGCAGGCATCGTCCCGCATGTGCGCGTGCATGCGCCGAATT
TTCCGGCCGTGATCCGCTGCGTGCAGGAGGGCGCCGGCATCGCACTGGTACCGCGTTCCGTGGCGGCACCGGCCATCGCG
CAAGGGCTCGTCGAGGGCGTGGTGCTGGACGAACCCTGGGCCATGCGCGAGCAGCGCGTGGTCGCGCGCAGCCTTCAGAG
CTTGCCGGACTACACGCGTGCGTTCATTCGCTTTCTGACGGAATCCAGCGAGTCATTCCCAGACACCGTCCTGCGTTAG

Upstream 100 bases:

>100_bases
CTTCGCGGGATAGCCAGCGTCAGCCGCTGCCGGCGTTGCGGTGGCCAGGAGCGCCCCGGCGCAGATCAGCGCTGCGCCGA
GCCGGTAAAGCGATGGAATC

Downstream 100 bases:

>100_bases
ATGTGTTTGAAGCCGTGTAAGGAAATATCGACTGGCTTCGTCACACACGTCACTCCATACTCGGCCATCCCGGACGCCGT
GCGTCCCACGCCTAATGGAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Als operon regulatory protein [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MRLSPSGSVVTGNVFAMSPCHGASDHYRIDSAARASRKVLSPLRRIRTAMPEAPLAPRHRPAPWQQETTLRFELTDLRVF
VAIAEAGSLTMGASDMHLTAPSASYRLKNLEQTVGVPLFNRTQKGMVLTAAGETMLQHARTILDNVEQLQIGMRRHTDGI
EGHIRVYANSSTMCNLPVALSRFLAAYPNVNVDLEEHLSADSVRAVQEQRADIGLVAGAIDLHGLDSILYGADELLFVVP
PRHPLGMRDSVSLVQALRHELVSIGSKSSNFLYLQDMATRAGIVPHVRVHAPNFPAVIRCVQEGAGIALVPRSVAAPAIA
QGLVEGVVLDEPWAMREQRVVARSLQSLPDYTRAFIRFLTESSESFPDTVLR

Sequences:

>Translated_372_residues
MRLSPSGSVVTGNVFAMSPCHGASDHYRIDSAARASRKVLSPLRRIRTAMPEAPLAPRHRPAPWQQETTLRFELTDLRVF
VAIAEAGSLTMGASDMHLTAPSASYRLKNLEQTVGVPLFNRTQKGMVLTAAGETMLQHARTILDNVEQLQIGMRRHTDGI
EGHIRVYANSSTMCNLPVALSRFLAAYPNVNVDLEEHLSADSVRAVQEQRADIGLVAGAIDLHGLDSILYGADELLFVVP
PRHPLGMRDSVSLVQALRHELVSIGSKSSNFLYLQDMATRAGIVPHVRVHAPNFPAVIRCVQEGAGIALVPRSVAAPAIA
QGLVEGVVLDEPWAMREQRVVARSLQSLPDYTRAFIRFLTESSESFPDTVLR
>Mature_372_residues
MRLSPSGSVVTGNVFAMSPCHGASDHYRIDSAARASRKVLSPLRRIRTAMPEAPLAPRHRPAPWQQETTLRFELTDLRVF
VAIAEAGSLTMGASDMHLTAPSASYRLKNLEQTVGVPLFNRTQKGMVLTAAGETMLQHARTILDNVEQLQIGMRRHTDGI
EGHIRVYANSSTMCNLPVALSRFLAAYPNVNVDLEEHLSADSVRAVQEQRADIGLVAGAIDLHGLDSILYGADELLFVVP
PRHPLGMRDSVSLVQALRHELVSIGSKSSNFLYLQDMATRAGIVPHVRVHAPNFPAVIRCVQEGAGIALVPRSVAAPAIA
QGLVEGVVLDEPWAMREQRVVARSLQSLPDYTRAFIRFLTESSESFPDTVLR

Specific function: Regulates the expression of the alsSD operon for acetoin biosynthesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=241, Percent_Identity=26.1410788381743, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787806, Length=272, Percent_Identity=27.2058823529412, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI145693105, Length=280, Percent_Identity=22.8571428571429, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788887, Length=242, Percent_Identity=27.2727272727273, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787879, Length=248, Percent_Identity=26.6129032258064, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI2367136, Length=253, Percent_Identity=28.0632411067194, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1786713, Length=266, Percent_Identity=27.4436090225564, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788481, Length=300, Percent_Identity=26.3333333333333, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790262, Length=287, Percent_Identity=25.7839721254355, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787601, Length=260, Percent_Identity=25, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790208, Length=246, Percent_Identity=23.9837398373984, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786508, Length=264, Percent_Identity=23.1060606060606, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081904, Length=195, Percent_Identity=28.2051282051282, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082132, Length=275, Percent_Identity=22.5454545454545, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787530, Length=258, Percent_Identity=22.8682170542636, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789204, Length=268, Percent_Identity=26.4925373134328, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 40671; Mature: 40671

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSPSGSVVTGNVFAMSPCHGASDHYRIDSAARASRKVLSPLRRIRTAMPEAPLAPRHR
CCCCCCCCEEECCEEEECCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PAPWQQETTLRFELTDLRVFVAIAEAGSLTMGASDMHLTAPSASYRLKNLEQTVGVPLFN
CCCCCCCCEEEEEEEEEEEEEEEECCCCEEECCCCCEEECCCHHHHHHHHHHHHCCCCCC
RTQKGMVLTAAGETMLQHARTILDNVEQLQIGMRRHTDGIEGHIRVYANSSTMCNLPVAL
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEECCHHHH
SRFLAAYPNVNVDLEEHLSADSVRAVQEQRADIGLVAGAIDLHGLDSILYGADELLFVVP
HHHHHHCCCCCCCHHHHCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCCEEEEEC
PRHPLGMRDSVSLVQALRHELVSIGSKSSNFLYLQDMATRAGIVPHVRVHAPNFPAVIRC
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHCCCCCEEEEECCCCHHHHHH
VQEGAGIALVPRSVAAPAIAQGLVEGVVLDEPWAMREQRVVARSLQSLPDYTRAFIRFLT
HHCCCCEEEECHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHCHHHHHHHHHHHH
ESSESFPDTVLR
HCCCCCCHHHCC
>Mature Secondary Structure
MRLSPSGSVVTGNVFAMSPCHGASDHYRIDSAARASRKVLSPLRRIRTAMPEAPLAPRHR
CCCCCCCCEEECCEEEECCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PAPWQQETTLRFELTDLRVFVAIAEAGSLTMGASDMHLTAPSASYRLKNLEQTVGVPLFN
CCCCCCCCEEEEEEEEEEEEEEEECCCCEEECCCCCEEECCCHHHHHHHHHHHHCCCCCC
RTQKGMVLTAAGETMLQHARTILDNVEQLQIGMRRHTDGIEGHIRVYANSSTMCNLPVAL
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEECCHHHH
SRFLAAYPNVNVDLEEHLSADSVRAVQEQRADIGLVAGAIDLHGLDSILYGADELLFVVP
HHHHHHCCCCCCCHHHHCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCCEEEEEC
PRHPLGMRDSVSLVQALRHELVSIGSKSSNFLYLQDMATRAGIVPHVRVHAPNFPAVIRC
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHCCCCCEEEEECCCCHHHHHH
VQEGAGIALVPRSVAAPAIAQGLVEGVVLDEPWAMREQRVVARSLQSLPDYTRAFIRFLT
HHCCCCEEEECHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHCHHHHHHHHHHHH
ESSESFPDTVLR
HCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377 [H]