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The map label for this gene is ywbI [H]

Identifier: 94312729

GI number: 94312729

Start: 339049

End: 339951

Strand: Reverse

Name: ywbI [H]

Synonym: Rmet_3799

Alternate gene names: 94312729

Gene position: 339951-339049 (Counterclockwise)

Preceding gene: 94312731

Following gene: 94312726

Centisome position: 13.18

GC content: 63.46

Gene sequence:

>903_bases
ATGCGCTTCGACCTGGTCGATCTGAATCTCTTCACGCATATCGCTGAGGCCAGCAGCCTGACCCGCGGTGCAGAACGTTG
CCACCTATCCCTGCCGGCTGCCAGCACCCGCATCAAGAACCTGGAAGAGCAGGTTGGCGTCAAACTGCTGAGCCGCAGCA
GCCTCGGCGTCAAGGTGACGCCGGCAGGCGCGACGCTGCTTGCGCATGCCCGGCGCGTGCTGCGCCAGATCGAGCAGTTG
AGCGGTGATCTGCAGGAGTATTCGTCCGGTATCAAGGGGCATGTGCGCGTATTCGCCAATACCACGGCGATGAGCGAGTT
CCTGCCCGGGGTGCTGCGTACGTACCTCGTCAATCACCCCGACGTAACGGTCGACATCCGGGAGCGCCTCAGTCCGGACA
TCGTCAGGGCCGTGCAGGAAGGCATGGTCGATATCGGCATCGCCGCCAGCGGCGTGAACATGGAAGGCCTGGAAGTGATG
CCCTATCGGCTCGACAGACTCGTGCTGGCGACTGCACTCAGCCATCCGTTTGCCCAGAAGAAGCGGGTGCGCTTCGTCGA
AACGCTCGACCAGGATTTCATCGGCCTGCCCGAGGAAAGCGCCATCCACAACTTCCTGAAGCGTGCTGCATCGGACCTGC
AGCGCAACCTGCGCTGGCGCATCCAGGTCAGCAACTTCGAAACCGCCGCGCGCATGATCGAGGCAAACGTGGGGATCGGC
GTGCTGCCGGAGAGCACCGCCCGCCGACTTTCCCAGACCATGGCGCTGAAGATCGTGCCGATCGAGGATGAGTGGGCCGA
GCGCAAGTTGCAGATCTGCGTGGCGGACCTGGACGCGCTACCCGTCTTCGCGCGCAAGCTGGTGGAACTGCTGGTCGAGG
ACGGCATCGGCAATCCGGTCTGA

Upstream 100 bases:

>100_bases
TCCTGCGTTCGCCTGCGCGGAAGTACCCCCCCGTAGTTGCGCGAATAGATCGAATGGGCCCGTTGGGGGGATAATCGCTG
ACTATTCTGGAGACAACGTC

Downstream 100 bases:

>100_bases
CGATTGCCGATGCCCCGAGCCGCTCATACCGCGGCTAGACCAGTTCCGCGATCATTCCCCGCAGGCGGTGCTTGTGCAGC
TTGCCGCTGGCCGTCGTCGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MRFDLVDLNLFTHIAEASSLTRGAERCHLSLPAASTRIKNLEEQVGVKLLSRSSLGVKVTPAGATLLAHARRVLRQIEQL
SGDLQEYSSGIKGHVRVFANTTAMSEFLPGVLRTYLVNHPDVTVDIRERLSPDIVRAVQEGMVDIGIAASGVNMEGLEVM
PYRLDRLVLATALSHPFAQKKRVRFVETLDQDFIGLPEESAIHNFLKRAASDLQRNLRWRIQVSNFETAARMIEANVGIG
VLPESTARRLSQTMALKIVPIEDEWAERKLQICVADLDALPVFARKLVELLVEDGIGNPV

Sequences:

>Translated_300_residues
MRFDLVDLNLFTHIAEASSLTRGAERCHLSLPAASTRIKNLEEQVGVKLLSRSSLGVKVTPAGATLLAHARRVLRQIEQL
SGDLQEYSSGIKGHVRVFANTTAMSEFLPGVLRTYLVNHPDVTVDIRERLSPDIVRAVQEGMVDIGIAASGVNMEGLEVM
PYRLDRLVLATALSHPFAQKKRVRFVETLDQDFIGLPEESAIHNFLKRAASDLQRNLRWRIQVSNFETAARMIEANVGIG
VLPESTARRLSQTMALKIVPIEDEWAERKLQICVADLDALPVFARKLVELLVEDGIGNPV
>Mature_300_residues
MRFDLVDLNLFTHIAEASSLTRGAERCHLSLPAASTRIKNLEEQVGVKLLSRSSLGVKVTPAGATLLAHARRVLRQIEQL
SGDLQEYSSGIKGHVRVFANTTAMSEFLPGVLRTYLVNHPDVTVDIRERLSPDIVRAVQEGMVDIGIAASGVNMEGLEVM
PYRLDRLVLATALSHPFAQKKRVRFVETLDQDFIGLPEESAIHNFLKRAASDLQRNLRWRIQVSNFETAARMIEANVGIG
VLPESTARRLSQTMALKIVPIEDEWAERKLQICVADLDALPVFARKLVELLVEDGIGNPV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693105, Length=243, Percent_Identity=27.1604938271605, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI157672245, Length=194, Percent_Identity=28.8659793814433, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787879, Length=271, Percent_Identity=25.4612546125461, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790399, Length=245, Percent_Identity=25.3061224489796, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI2367136, Length=248, Percent_Identity=25.4032258064516, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787530, Length=232, Percent_Identity=26.2931034482759, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786713, Length=273, Percent_Identity=26.7399267399267, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788297, Length=245, Percent_Identity=26.9387755102041, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081904, Length=202, Percent_Identity=30.1980198019802, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788887, Length=185, Percent_Identity=28.1081081081081, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790208, Length=278, Percent_Identity=25.8992805755396, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790262, Length=232, Percent_Identity=25.8620689655172, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788748, Length=147, Percent_Identity=29.9319727891156, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI145693193, Length=126, Percent_Identity=32.5396825396825, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788481, Length=179, Percent_Identity=26.8156424581006, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789204, Length=244, Percent_Identity=22.5409836065574, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787601, Length=138, Percent_Identity=28.2608695652174, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787589, Length=125, Percent_Identity=29.6, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33262; Mature: 33262

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDLVDLNLFTHIAEASSLTRGAERCHLSLPAASTRIKNLEEQVGVKLLSRSSLGVKVT
CCEEEEEHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEC
PAGATLLAHARRVLRQIEQLSGDLQEYSSGIKGHVRVFANTTAMSEFLPGVLRTYLVNHP
CCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCC
DVTVDIRERLSPDIVRAVQEGMVDIGIAASGVNMEGLEVMPYRLDRLVLATALSHPFAQK
CEEEEHHHHCCHHHHHHHHHCCEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHCCCHHHH
KRVRFVETLDQDFIGLPEESAIHNFLKRAASDLQRNLRWRIQVSNFETAARMIEANVGIG
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHCCCCE
VLPESTARRLSQTMALKIVPIEDEWAERKLQICVADLDALPVFARKLVELLVEDGIGNPV
ECCCHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRFDLVDLNLFTHIAEASSLTRGAERCHLSLPAASTRIKNLEEQVGVKLLSRSSLGVKVT
CCEEEEEHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEC
PAGATLLAHARRVLRQIEQLSGDLQEYSSGIKGHVRVFANTTAMSEFLPGVLRTYLVNHP
CCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCC
DVTVDIRERLSPDIVRAVQEGMVDIGIAASGVNMEGLEVMPYRLDRLVLATALSHPFAQK
CEEEEHHHHCCHHHHHHHHHCCEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHCCCHHHH
KRVRFVETLDQDFIGLPEESAIHNFLKRAASDLQRNLRWRIQVSNFETAARMIEANVGIG
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHCCCCE
VLPESTARRLSQTMALKIVPIEDEWAERKLQICVADLDALPVFARKLVELLVEDGIGNPV
ECCCHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]