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The map label for this gene is alkB1 [H]

Identifier: 94312699

GI number: 94312699

Start: 305913

End: 306497

Strand: Reverse

Name: alkB1 [H]

Synonym: Rmet_3769

Alternate gene names: 94312699

Gene position: 306497-305913 (Counterclockwise)

Preceding gene: 291481491

Following gene: 94312698

Centisome position: 11.88

GC content: 62.74

Gene sequence:

>585_bases
GTGGTGCTGCGCGGATTCGCGCTGCCTTATGTGCCCGATCTGATGCCTGCCATTGCGGGCATCGAGACGACTTCGCCCTT
TCGGCATATGGTCACGCCGGGCGGTTTCACCATGTCGGTGGCGCTAACGAATTGCGGCGCGTTGGGCTGGACGACCGACC
GGCGCGGCTATCGCTACACCACCGTTGATCCCGATACGGGAAAGCCCTGGCCGGTCATGCCGGAGGTATTCTTTCGGCTG
GCAAATGAGGCTGCTGCTGAGGCGGGCTTTGACGACTTCGAGCCCGACGCCTGCCTGCTCAATCGCTACCTGCCTGGCAG
CAGACTTGCGCTGCACCAGGACAAGAACGAGCAAGCCTATGAGACGCCGATCGTTTCGGTATCTCTGGGCATGCGGGCTA
CGTTCCTGTTCGGCGGTCATGCGCGCACCGCCCCCACGATCAAGGTACCGCTCCACCACGGGGACGTAGTGGTGTGGGGC
GGCGCCGATCGTCTGCGCTACCACGGCGTAATGCCGATCAAGGATGCACCTCATGCGCTGCTGGGCAGCCAGCGCATCAA
TTTCACTTTCCGAAAAGCGGCCTAG

Upstream 100 bases:

>100_bases
GCCACACTGTGGGTCTGATCTTTCACGATGCAAGGTGAGCATGGGAACGCTCGAACTCTTTGAGGAAACTGCGGAGCAAC
ACCGTCTCGGTCCTGCCGCA

Downstream 100 bases:

>100_bases
CTAGCTCCTGGAATTCAACCGCAAGAGACGGAGTTTCCTCCCGCGGTGGATTCACTAGAGTGACAGTAGCAGCCGAGGTT
TCCTATGAGCAGGATTTCCG

Product: alpha-ketoglutarate-dependent dioxygenase alkB

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 194; Mature: 194

Protein sequence:

>194_residues
MVLRGFALPYVPDLMPAIAGIETTSPFRHMVTPGGFTMSVALTNCGALGWTTDRRGYRYTTVDPDTGKPWPVMPEVFFRL
ANEAAAEAGFDDFEPDACLLNRYLPGSRLALHQDKNEQAYETPIVSVSLGMRATFLFGGHARTAPTIKVPLHHGDVVVWG
GADRLRYHGVMPIKDAPHALLGSQRINFTFRKAA

Sequences:

>Translated_194_residues
MVLRGFALPYVPDLMPAIAGIETTSPFRHMVTPGGFTMSVALTNCGALGWTTDRRGYRYTTVDPDTGKPWPVMPEVFFRL
ANEAAAEAGFDDFEPDACLLNRYLPGSRLALHQDKNEQAYETPIVSVSLGMRATFLFGGHARTAPTIKVPLHHGDVVVWG
GADRLRYHGVMPIKDAPHALLGSQRINFTFRKAA
>Mature_194_residues
MVLRGFALPYVPDLMPAIAGIETTSPFRHMVTPGGFTMSVALTNCGALGWTTDRRGYRYTTVDPDTGKPWPVMPEVFFRL
ANEAAAEAGFDDFEPDACLLNRYLPGSRLALHQDKNEQAYETPIVSVSLGMRATFLFGGHARTAPTIKVPLHHGDVVVWG
GADRLRYHGVMPIKDAPHALLGSQRINFTFRKAA

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=91, Percent_Identity=37.3626373626374, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1788541, Length=193, Percent_Identity=55.9585492227979, Blast_Score=228, Evalue=2e-61,
Organism=Drosophila melanogaster, GI45555401, Length=125, Percent_Identity=34.4, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004574 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 21315; Mature: 21315

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLRGFALPYVPDLMPAIAGIETTSPFRHMVTPGGFTMSVALTNCGALGWTTDRRGYRYT
CEECCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCEEEEEEEECCCCCCCCCCCCCEEEE
TVDPDTGKPWPVMPEVFFRLANEAAAEAGFDDFEPDACLLNRYLPGSRLALHQDKNEQAY
EECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCHH
ETPIVSVSLGMRATFLFGGHARTAPTIKVPLHHGDVVVWGGADRLRYHGVMPIKDAPHAL
HCCEEEEECCCEEEEEECCCCCCCCEEEEEECCCCEEEECCCCCEEECCCCCCCCCCHHH
LGSQRINFTFRKAA
HCCCEEEEEEEECC
>Mature Secondary Structure
MVLRGFALPYVPDLMPAIAGIETTSPFRHMVTPGGFTMSVALTNCGALGWTTDRRGYRYT
CEECCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCEEEEEEEECCCCCCCCCCCCCEEEE
TVDPDTGKPWPVMPEVFFRLANEAAAEAGFDDFEPDACLLNRYLPGSRLALHQDKNEQAY
EECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCHH
ETPIVSVSLGMRATFLFGGHARTAPTIKVPLHHGDVVVWGGADRLRYHGVMPIKDAPHAL
HCCEEEEECCCEEEEEECCCCCCCCEEEEEECCCCEEEECCCCCEEECCCCCCCCCCHHH
LGSQRINFTFRKAA
HCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536913; 9097040; 9278503; 3887409; 7928996 [H]