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The map label for this gene is pcaJ [H]

Identifier: 94312600

GI number: 94312600

Start: 205660

End: 206307

Strand: Direct

Name: pcaJ [H]

Synonym: Rmet_3668

Alternate gene names: 94312600

Gene position: 205660-206307 (Clockwise)

Preceding gene: 94312599

Following gene: 94312601

Centisome position: 7.97

GC content: 66.51

Gene sequence:

>648_bases
ATGCAACGCCTGACCCGCGACCAAATGGCCGCCCGCGTGGCCAAAGACATTCCGGACGGTGCCGTGGTGAACCTCGGCAT
CGGCCTGCCCACCCTGGTCGGCAATCAACTGCCGGCCGACCGTGAAATCCTGCTGCACAGCGAGAACGGCCTGCTCGGCA
TGGGCCCCGCTCCCGCGCCGGGCGAGGAAGACGGCGACCTGATCAACGCTGGCAAGCAGCCGGTGACGATCAAGCCGGGT
GGCTCGTATTTCCACCACGCCGATTCGTTCGCGATGATGCGTGGCGGCCACCTCGACTTCTGCGTGCTGGGCGCGTTCCA
GGTATCGGAGAAGGGTGACCTGGCCAACTGGCATACCGGCGCACCGGGCGCCATCCCCGCCGTGGGCGGTGCGATGGACC
TGGCTATCGGCGCCAAGCAGGTGTTCGTGATGATGGAGCACCTGACCAAGCAGGGCGAAAGCAAGATCGTGTCCCAGTGC
ACCTATCCGCTGACCGGCATCGGCTGCGTCACGCGTGTCTACACGGACCTGGCCACGATCGATGTGACGCCGGACGGCCT
GGTTGCCCGCGACCTCGTGGACGGCCTGTCGTTCGACGAACTGCAACGCCTGACCGGCGTGCCTCTCAAGCAGGCCGTAG
CCGCCTGA

Upstream 100 bases:

>100_bases
ATCACGCCGGGTCTGTTCGTCAAGCGCGTGGTCAAGGTTGCAAGCGCCGCCAAGCCGGCCAGCGCGGCCTGATCCGAATC
GAGCGAAGAAGGAGAAACAC

Downstream 100 bases:

>100_bases
CCAACCACCAAGACGAGCGAGACAGAACATGAGCGAAGCATTCATCGTTGACGCCATCCGTACCCCCATCGGCCGTTACG
CCGGCAGCCTGTCCGGCGTG

Product: 3-oxoacid CoA-transferase subunit B

Products: NA

Alternate protein names: Beta-ketoadipate:succinyl-CoA transferase subunit B [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MQRLTRDQMAARVAKDIPDGAVVNLGIGLPTLVGNQLPADREILLHSENGLLGMGPAPAPGEEDGDLINAGKQPVTIKPG
GSYFHHADSFAMMRGGHLDFCVLGAFQVSEKGDLANWHTGAPGAIPAVGGAMDLAIGAKQVFVMMEHLTKQGESKIVSQC
TYPLTGIGCVTRVYTDLATIDVTPDGLVARDLVDGLSFDELQRLTGVPLKQAVAA

Sequences:

>Translated_215_residues
MQRLTRDQMAARVAKDIPDGAVVNLGIGLPTLVGNQLPADREILLHSENGLLGMGPAPAPGEEDGDLINAGKQPVTIKPG
GSYFHHADSFAMMRGGHLDFCVLGAFQVSEKGDLANWHTGAPGAIPAVGGAMDLAIGAKQVFVMMEHLTKQGESKIVSQC
TYPLTGIGCVTRVYTDLATIDVTPDGLVARDLVDGLSFDELQRLTGVPLKQAVAA
>Mature_215_residues
MQRLTRDQMAARVAKDIPDGAVVNLGIGLPTLVGNQLPADREILLHSENGLLGMGPAPAPGEEDGDLINAGKQPVTIKPG
GSYFHHADSFAMMRGGHLDFCVLGAFQVSEKGDLANWHTGAPGAIPAVGGAMDLAIGAKQVFVMMEHLTKQGESKIVSQC
TYPLTGIGCVTRVYTDLATIDVTPDGLVARDLVDGLSFDELQRLTGVPLKQAVAA

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=204, Percent_Identity=49.0196078431373, Blast_Score=185, Evalue=3e-47,
Organism=Homo sapiens, GI11545841, Length=204, Percent_Identity=48.5294117647059, Blast_Score=184, Evalue=6e-47,
Organism=Escherichia coli, GI1788552, Length=201, Percent_Identity=44.2786069651741, Blast_Score=167, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17531517, Length=210, Percent_Identity=47.6190476190476, Blast_Score=185, Evalue=1e-47,
Organism=Drosophila melanogaster, GI21358325, Length=207, Percent_Identity=44.4444444444444, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24655794, Length=207, Percent_Identity=44.4444444444444, Blast_Score=171, Evalue=2e-43,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.6 [H]

Molecular weight: Translated: 22539; Mature: 22539

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRLTRDQMAARVAKDIPDGAVVNLGIGLPTLVGNQLPADREILLHSENGLLGMGPAPAP
CCCCHHHHHHHHHHHCCCCCCEEEECCCCHHHHCCCCCCCCEEEEECCCCEEECCCCCCC
GEEDGDLINAGKQPVTIKPGGSYFHHADSFAMMRGGHLDFCVLGAFQVSEKGDLANWHTG
CCCCCCEECCCCCCEEECCCCCCHHCCCCHHHHCCCCCCEEEEEEEEECCCCCCCCCCCC
APGAIPAVGGAMDLAIGAKQVFVMMEHLTKQGESKIVSQCTYPLTGIGCVTRVYTDLATI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHEEE
DVTPDGLVARDLVDGLSFDELQRLTGVPLKQAVAA
ECCCCCHHHHHHHCCCCHHHHHHHHCCCHHHHHCC
>Mature Secondary Structure
MQRLTRDQMAARVAKDIPDGAVVNLGIGLPTLVGNQLPADREILLHSENGLLGMGPAPAP
CCCCHHHHHHHHHHHCCCCCCEEEECCCCHHHHCCCCCCCCEEEEECCCCEEECCCCCCC
GEEDGDLINAGKQPVTIKPGGSYFHHADSFAMMRGGHLDFCVLGAFQVSEKGDLANWHTG
CCCCCCEECCCCCCEEECCCCCCHHCCCCHHHHCCCCCCEEEEEEEEECCCCCCCCCCCC
APGAIPAVGGAMDLAIGAKQVFVMMEHLTKQGESKIVSQCTYPLTGIGCVTRVYTDLATI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHEEE
DVTPDGLVARDLVDGLSFDELQRLTGVPLKQAVAA
ECCCCCHHHHHHHCCCCHHHHHHHHCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]