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The map label for this gene is pcaR [H]

Identifier: 94312571

GI number: 94312571

Start: 173941

End: 174852

Strand: Reverse

Name: pcaR [H]

Synonym: Rmet_3639

Alternate gene names: 94312571

Gene position: 174852-173941 (Counterclockwise)

Preceding gene: 94312573

Following gene: 94312566

Centisome position: 6.78

GC content: 65.02

Gene sequence:

>912_bases
ATGCGTCAGACTGGCAAAGCCGGCGCATTTCCTCTACCCTTTCGGCCTTTCCGAGCCAATTCCGGCGCATCGACGCCGAT
GTTCCGATCGATGGCGAACCCAGCCGATACCGCAACGTCCCGCGCGCCCGTAGCCGGCGCCACCGAAAAGCCAAGCGACA
GCTACGTGCAGTCCTTCGCACGCGGCCTGTCGGTGATTCGCGCCTTCAATGCCAGCCACCCCGCGCAAACGCTGACCGAG
ATCGCCCAGGCCAGTGGCCTGACGCGCGCCGGCGCGCGACGCATCCTGCTCACGCTGGTGGGGCTCGGCTATGTGCAGAA
CGACGGCCGGCTGTTCCGGCTCACGCCAAAGATCCTCGATCTCGGCTTCGCCTATCTGACGTCGATGCCGTTCTGGAATC
TGGCCGAGCCGATCATGGAGGCGCTGTCGCAGTCGGTCCATGAAAGCTGCTCGATCTCGGTGCTGGACGGCACGGAAATC
GTCTATGTGCTGCGCGTGCCCGCACGCAAGATCATGACGATCAACCTGTCGATCGGCAGCCGCCTGCCGGCCTACTGTTC
GTCGATGGGACGCGTGCTGCTGTCGGGACTGGATCAGGAAGAACTCGATCGCGTGCTGCACGCGTCGGACCTGCGGCCGC
GCACGCGCCGCACCGTGACCGATATCGGCGAATTGAAGACGCTGATTGGCGAGATTCGCGAGCGCGGCTGGGCGCAGAAC
GACCAGGAGTTGGAGGAGGGGCTGGTTTCGCTGTCCGCCCCGATCCGGAATCGCGGCGGTCAGGTCATCGCCGCGATCAA
CATCAGCGGCCAGGCCAATCGCACGAGCGCGCAAGAGATGGTGGAGCGCTTCCTGCCGCCGCTGCTGGAAGCAGCGGGCA
AGATTTCAGCGATGGTGGGGCTGCGTACCTGA

Upstream 100 bases:

>100_bases
TTGTTGTCCCGACTATCTGTCCACCATTGTGCGGACGCACATTATGCGATAGACAGATATGTCTCGTCAGCGTGCCCGGG
CACCCCGGTACCGGCCGTAA

Downstream 100 bases:

>100_bases
GGGCCAGGGCGGGGCCCGCCCCCGGCGGTTACCAGGAAACCGCGATATCCCCCTTCACGAACGTCTGGCAAGCCATGCGA
TAGCCAGCGCGCAGATCGTC

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MRQTGKAGAFPLPFRPFRANSGASTPMFRSMANPADTATSRAPVAGATEKPSDSYVQSFARGLSVIRAFNASHPAQTLTE
IAQASGLTRAGARRILLTLVGLGYVQNDGRLFRLTPKILDLGFAYLTSMPFWNLAEPIMEALSQSVHESCSISVLDGTEI
VYVLRVPARKIMTINLSIGSRLPAYCSSMGRVLLSGLDQEELDRVLHASDLRPRTRRTVTDIGELKTLIGEIRERGWAQN
DQELEEGLVSLSAPIRNRGGQVIAAINISGQANRTSAQEMVERFLPPLLEAAGKISAMVGLRT

Sequences:

>Translated_303_residues
MRQTGKAGAFPLPFRPFRANSGASTPMFRSMANPADTATSRAPVAGATEKPSDSYVQSFARGLSVIRAFNASHPAQTLTE
IAQASGLTRAGARRILLTLVGLGYVQNDGRLFRLTPKILDLGFAYLTSMPFWNLAEPIMEALSQSVHESCSISVLDGTEI
VYVLRVPARKIMTINLSIGSRLPAYCSSMGRVLLSGLDQEELDRVLHASDLRPRTRRTVTDIGELKTLIGEIRERGWAQN
DQELEEGLVSLSAPIRNRGGQVIAAINISGQANRTSAQEMVERFLPPLLEAAGKISAMVGLRT
>Mature_303_residues
MRQTGKAGAFPLPFRPFRANSGASTPMFRSMANPADTATSRAPVAGATEKPSDSYVQSFARGLSVIRAFNASHPAQTLTE
IAQASGLTRAGARRILLTLVGLGYVQNDGRLFRLTPKILDLGFAYLTSMPFWNLAEPIMEALSQSVHESCSISVLDGTEI
VYVLRVPARKIMTINLSIGSRLPAYCSSMGRVLLSGLDQEELDRVLHASDLRPRTRRTVTDIGELKTLIGEIRERGWAQN
DQELEEGLVSLSAPIRNRGGQVIAAINISGQANRTSAQEMVERFLPPLLEAAGKISAMVGLRT

Specific function: Positive regulator of all genes within the pca regulon, pcaBDC, pcaIJ and pcaF. Also required for the chemotactic response to aromatic compounds [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1788131, Length=225, Percent_Identity=31.5555555555556, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87082359, Length=274, Percent_Identity=28.1021897810219, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1789999, Length=263, Percent_Identity=27.3764258555133, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1786468, Length=248, Percent_Identity=27.4193548387097, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786715, Length=259, Percent_Identity=25.0965250965251, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790752, Length=211, Percent_Identity=27.9620853080569, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012794
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 32851; Mature: 32851

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQTGKAGAFPLPFRPFRANSGASTPMFRSMANPADTATSRAPVAGATEKPSDSYVQSFA
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHCCCCCCCCCCCCCCCHHHHHHHH
RGLSVIRAFNASHPAQTLTEIAQASGLTRAGARRILLTLVGLGYVQNDGRLFRLTPKILD
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCEEECCCEEEEECHHHHH
LGFAYLTSMPFWNLAEPIMEALSQSVHESCSISVLDGTEIVYVLRVPARKIMTINLSIGS
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCEEEEECCCEEEEEEECCCCEEEEEEECCCC
RLPAYCSSMGRVLLSGLDQEELDRVLHASDLRPRTRRTVTDIGELKTLIGEIRERGWAQN
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
DQELEEGLVSLSAPIRNRGGQVIAAINISGQANRTSAQEMVERFLPPLLEAAGKISAMVG
HHHHHHHHHHHHCHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
LRT
CCC
>Mature Secondary Structure
MRQTGKAGAFPLPFRPFRANSGASTPMFRSMANPADTATSRAPVAGATEKPSDSYVQSFA
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHCCCCCCCCCCCCCCCHHHHHHHH
RGLSVIRAFNASHPAQTLTEIAQASGLTRAGARRILLTLVGLGYVQNDGRLFRLTPKILD
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCEEECCCEEEEECHHHHH
LGFAYLTSMPFWNLAEPIMEALSQSVHESCSISVLDGTEIVYVLRVPARKIMTINLSIGS
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCEEEEECCCEEEEEEECCCCEEEEEEECCCC
RLPAYCSSMGRVLLSGLDQEELDRVLHASDLRPRTRRTVTDIGELKTLIGEIRERGWAQN
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
DQELEEGLVSLSAPIRNRGGQVIAAINISGQANRTSAQEMVERFLPPLLEAAGKISAMVG
HHHHHHHHHHHHCHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
LRT
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8083169 [H]