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The map label for this gene is pckG [H]

Identifier: 94312508

GI number: 94312508

Start: 3880395

End: 3882251

Strand: Reverse

Name: pckG [H]

Synonym: Rmet_3577

Alternate gene names: 94312508

Gene position: 3882251-3880395 (Counterclockwise)

Preceding gene: 94312515

Following gene: 94312505

Centisome position: 98.83

GC content: 65.32

Gene sequence:

>1857_bases
ATGAACCACCCCACAATGCAAGGCACTCCGGCACTGAACGTGCCGGCTTGGGTCAAGAACCAGAAACTGGTCGCCTGGGT
TGCCGACATCGCCGCATTGACCAAGCCCGACCAGGTCTACTGGTGCGATGGCTCGCAGGAAGAATATGACCGCCTGTGCG
AGCAGATGGTCGCCGCCGGCACGATGCGCCGCCTGAACCCGGCCAAGCGCAAGAACTCGTTCCTGGCCCTGTCGGATCCC
TCCGATGTGGCACGTGTCGAAGACCGCACTTTCATCTGCTCGCAGAAGCAGGAAGACGCCGGCCCGACCAACAACTGGAC
CGCACCGGCCGAAATGCGCCAGACGCTGAACGGCCTGTTCGACGGCTGCATGCGTGGCCGCACCCTGTACGTGGTGCCGT
TCTCGATGGGCCCGCTGGGTTCGCCGATCGCCCACATCGGCGTCGAGCTGTCCGATTCGCCGTACGTCGCCGTGAACATG
CGCATCATGACCCGCATGGGCCGCGCCGTGTACGACGTGCTGGGTGCCGACGGCGAGTTCGTGCCGTGCGTGCATACCGT
GGGCAAGCCTCTGGCCGCCGGCGAGAAGGACGTGGCATGGCCGTGCAATCCGACCAAGTACATCGTGCACTTCCCCGAGA
CGCGCGAAATCTGGTCGTTCGGTTCGGGCTACGGCGGCAATGCGCTGCTGGGCAAGAAGTGCTTCGCACTGCGGATCGCC
TCGACGATGGGCCGCGACGAAGGCTGGCTGGCCGAGCACATGCTGATCCTCGGCGTGACCTCGCCGGAAGGCAAGAAGTA
CCACGTGGCTGCCGCGTTCCCGTCGGCCTGCGGCAAGACCAACTTCGCCATGCTGATCCCGCCCAAGGGCTTCGAGGGCT
GGAAGGTCACGACGATCGGTGACGACATCGCCTGGATCAAGCCAGGCAAGGACGGCCGCCTGTACGCCATCAACCCGGAA
GCCGGCTACTTCGGCGTGGCCCCGGGCACGAGCGAGAAGACCAACTTCAACGCGATGGCGACGCTCAAGGAAAACGTCAT
CTTCACCAACGTGGCACTGACCGACGACGGCGACGTGTGGTGGGAAGGCATGACGAAGGAAGCCCCGGCGCACCTGATCG
ACTGGCAGGGCAAGGACTGGACGCCCGAGATCGCCAAGTCGACCGGCGCCAAGGCTGCCCACCCGAACGCCCGCTTCACC
GCGCCGGCCTCGCAATGCCCGTCGATCGACGACAACTGGGACAACCCGGCTGGCGTGGCCATCGATGCATTCATCTTCGG
CGGCCGCCGCAGCTCCACCGTGCCACTGGTGACCGAAGCCCGCAACTGGACCGAAGGCGTCTACATGGCCGCCACGATGG
GTTCGGAAACCACCGCCGCCGCTGCCGGCCAGCAAGGCGTGGTCCGCCGCGATCCGTTCGCGATGCTGCCGTTCTGCGGC
TACAACATGAGCGACTACTTCGGCCACTGGCTGGCGTTGGGCAAGAAGCTCGAAGCCGCCGGTGCCAAGCTGCCGCGCAT
CTACTGCGTGAACTGGTTCCGCAAGGACGCCGACGGCAACTTCGTGTGGCCGGGCTTTGGCGAGAACATGCGCGTGCTGT
CGTGGATGATCGACCGCGTGGAAGGCCGTGGCGAAGGCGCCGAGCACGTGTTCGGCACGTCGCCGCGCTACACGGACCTG
AACTGGAACGGCCTGGCCTTCACCGCCGAGCAATTCACCCAGGTCACGTCGATCGACCGTGACGCATGGCAAAAGGAACT
GGCGCTGCACGACGAACTGTTCACGCAGCTCAAGCACAACCTGCCGCAAGCGCTGGCGGAAACCAAGGACGCACTGGCCA
AGCGCCTGTCGGCCTGA

Upstream 100 bases:

>100_bases
CAAGGCAGGCAGCCGGCCCGGCCCCGACTTTCCCGGCCCGCTCATCCGCGAACCGCCGCTCGGACATTGCACGATTTCTT
TTTCGCTTTGGAGTCGCAGT

Downstream 100 bases:

>100_bases
GTCTTCCCGCGTTAAGGAAAAACGCCGAACGGCTTGCCGCCGTTCGGCGTTTTTTTGTTTGGGCTATCGTTTTCTCGTGA
ACTGCCCCTCAGCCACCCTC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MNHPTMQGTPALNVPAWVKNQKLVAWVADIAALTKPDQVYWCDGSQEEYDRLCEQMVAAGTMRRLNPAKRKNSFLALSDP
SDVARVEDRTFICSQKQEDAGPTNNWTAPAEMRQTLNGLFDGCMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVAVNM
RIMTRMGRAVYDVLGADGEFVPCVHTVGKPLAAGEKDVAWPCNPTKYIVHFPETREIWSFGSGYGGNALLGKKCFALRIA
STMGRDEGWLAEHMLILGVTSPEGKKYHVAAAFPSACGKTNFAMLIPPKGFEGWKVTTIGDDIAWIKPGKDGRLYAINPE
AGYFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVWWEGMTKEAPAHLIDWQGKDWTPEIAKSTGAKAAHPNARFT
APASQCPSIDDNWDNPAGVAIDAFIFGGRRSSTVPLVTEARNWTEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLPFCG
YNMSDYFGHWLALGKKLEAAGAKLPRIYCVNWFRKDADGNFVWPGFGENMRVLSWMIDRVEGRGEGAEHVFGTSPRYTDL
NWNGLAFTAEQFTQVTSIDRDAWQKELALHDELFTQLKHNLPQALAETKDALAKRLSA

Sequences:

>Translated_618_residues
MNHPTMQGTPALNVPAWVKNQKLVAWVADIAALTKPDQVYWCDGSQEEYDRLCEQMVAAGTMRRLNPAKRKNSFLALSDP
SDVARVEDRTFICSQKQEDAGPTNNWTAPAEMRQTLNGLFDGCMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVAVNM
RIMTRMGRAVYDVLGADGEFVPCVHTVGKPLAAGEKDVAWPCNPTKYIVHFPETREIWSFGSGYGGNALLGKKCFALRIA
STMGRDEGWLAEHMLILGVTSPEGKKYHVAAAFPSACGKTNFAMLIPPKGFEGWKVTTIGDDIAWIKPGKDGRLYAINPE
AGYFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVWWEGMTKEAPAHLIDWQGKDWTPEIAKSTGAKAAHPNARFT
APASQCPSIDDNWDNPAGVAIDAFIFGGRRSSTVPLVTEARNWTEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLPFCG
YNMSDYFGHWLALGKKLEAAGAKLPRIYCVNWFRKDADGNFVWPGFGENMRVLSWMIDRVEGRGEGAEHVFGTSPRYTDL
NWNGLAFTAEQFTQVTSIDRDAWQKELALHDELFTQLKHNLPQALAETKDALAKRLSA
>Mature_618_residues
MNHPTMQGTPALNVPAWVKNQKLVAWVADIAALTKPDQVYWCDGSQEEYDRLCEQMVAAGTMRRLNPAKRKNSFLALSDP
SDVARVEDRTFICSQKQEDAGPTNNWTAPAEMRQTLNGLFDGCMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVAVNM
RIMTRMGRAVYDVLGADGEFVPCVHTVGKPLAAGEKDVAWPCNPTKYIVHFPETREIWSFGSGYGGNALLGKKCFALRIA
STMGRDEGWLAEHMLILGVTSPEGKKYHVAAAFPSACGKTNFAMLIPPKGFEGWKVTTIGDDIAWIKPGKDGRLYAINPE
AGYFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVWWEGMTKEAPAHLIDWQGKDWTPEIAKSTGAKAAHPNARFT
APASQCPSIDDNWDNPAGVAIDAFIFGGRRSSTVPLVTEARNWTEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLPFCG
YNMSDYFGHWLALGKKLEAAGAKLPRIYCVNWFRKDADGNFVWPGFGENMRVLSWMIDRVEGRGEGAEHVFGTSPRYTDL
NWNGLAFTAEQFTQVTSIDRDAWQKELALHDELFTQLKHNLPQALAETKDALAKRLSA

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI187281517, Length=610, Percent_Identity=50.4918032786885, Blast_Score=605, Evalue=1e-173,
Organism=Homo sapiens, GI66346721, Length=606, Percent_Identity=51.1551155115512, Blast_Score=595, Evalue=1e-170,
Organism=Homo sapiens, GI66346723, Length=378, Percent_Identity=53.968253968254, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17555492, Length=623, Percent_Identity=48.314606741573, Blast_Score=568, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI71989645, Length=612, Percent_Identity=47.0588235294118, Blast_Score=561, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI71989658, Length=612, Percent_Identity=47.0588235294118, Blast_Score=561, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI71989653, Length=599, Percent_Identity=47.4123539232053, Blast_Score=554, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI32564411, Length=563, Percent_Identity=50.7992895204263, Blast_Score=548, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI32564407, Length=531, Percent_Identity=51.0357815442561, Blast_Score=518, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI71989649, Length=486, Percent_Identity=50.2057613168724, Blast_Score=488, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI32564409, Length=377, Percent_Identity=48.0106100795756, Blast_Score=330, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI17540956, Length=523, Percent_Identity=25.4302103250478, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24655085, Length=605, Percent_Identity=49.7520661157025, Blast_Score=587, Evalue=1e-168,
Organism=Drosophila melanogaster, GI24655082, Length=606, Percent_Identity=49.009900990099, Blast_Score=573, Evalue=1e-164,
Organism=Drosophila melanogaster, GI161077226, Length=576, Percent_Identity=50, Blast_Score=555, Evalue=1e-158,
Organism=Drosophila melanogaster, GI24655088, Length=509, Percent_Identity=51.2770137524558, Blast_Score=514, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 67886; Mature: 67886

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00505 PEPCK_GTP ; PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHPTMQGTPALNVPAWVKNQKLVAWVADIAALTKPDQVYWCDGSQEEYDRLCEQMVAAG
CCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHH
TMRRLNPAKRKNSFLALSDPSDVARVEDRTFICSQKQEDAGPTNNWTAPAEMRQTLNGLF
HHHHCCHHHHCCCEEEECCCCHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
DGCMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVAVNMRIMTRMGRAVYDVLGADGEF
HHHHCCCEEEEEEECCCCCCCCHHHEEEEECCCCEEEEEHHHHHHHHHHHHHHHCCCCCE
VPCVHTVGKPLAAGEKDVAWPCNPTKYIVHFPETREIWSFGSGYGGNALLGKKCFALRIA
EHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCCCCCCHHCCHHHEEHHH
STMGRDEGWLAEHMLILGVTSPEGKKYHVAAAFPSACGKTNFAMLIPPKGFEGWKVTTIG
HHCCCCCCHHEEEEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCEEEEEC
DDIAWIKPGKDGRLYAINPEAGYFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVW
CCEEEECCCCCCEEEEECCCCCEEECCCCCCCCCCCCEEEEEECCEEEEEEEEECCCCEE
WEGMTKEAPAHLIDWQGKDWTPEIAKSTGAKAAHPNARFTAPASQCPSIDDNWDNPAGVA
ECCCCCCCCCCEEECCCCCCCHHHHHCCCCCCCCCCCEEECCHHHCCCCCCCCCCCCCEE
IDAFIFGGRRSSTVPLVTEARNWTEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLPFCG
EEEEEECCCCCCCCCEEECCCCCCCCEEEEEECCCCCHHHHCCCCCCEECCCCEECCCCC
YNMSDYFGHWLALGKKLEAAGAKLPRIYCVNWFRKDADGNFVWPGFGENMRVLSWMIDRV
CCHHHHHHHHHHHCCHHHHCCCCCCEEEEEEHEEECCCCCEECCCCCCCHHHHHHHHHHH
EGRGEGAEHVFGTSPRYTDLNWNGLAFTAEQFTQVTSIDRDAWQKELALHDELFTQLKHN
CCCCCCCCCCCCCCCCEECCCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LPQALAETKDALAKRLSA
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNHPTMQGTPALNVPAWVKNQKLVAWVADIAALTKPDQVYWCDGSQEEYDRLCEQMVAAG
CCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHH
TMRRLNPAKRKNSFLALSDPSDVARVEDRTFICSQKQEDAGPTNNWTAPAEMRQTLNGLF
HHHHCCHHHHCCCEEEECCCCHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
DGCMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVAVNMRIMTRMGRAVYDVLGADGEF
HHHHCCCEEEEEEECCCCCCCCHHHEEEEECCCCEEEEEHHHHHHHHHHHHHHHCCCCCE
VPCVHTVGKPLAAGEKDVAWPCNPTKYIVHFPETREIWSFGSGYGGNALLGKKCFALRIA
EHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCCCCCCHHCCHHHEEHHH
STMGRDEGWLAEHMLILGVTSPEGKKYHVAAAFPSACGKTNFAMLIPPKGFEGWKVTTIG
HHCCCCCCHHEEEEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCEEEEEC
DDIAWIKPGKDGRLYAINPEAGYFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVW
CCEEEECCCCCCEEEEECCCCCEEECCCCCCCCCCCCEEEEEECCEEEEEEEEECCCCEE
WEGMTKEAPAHLIDWQGKDWTPEIAKSTGAKAAHPNARFTAPASQCPSIDDNWDNPAGVA
ECCCCCCCCCCEEECCCCCCCHHHHHCCCCCCCCCCCEEECCHHHCCCCCCCCCCCCCEE
IDAFIFGGRRSSTVPLVTEARNWTEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLPFCG
EEEEEECCCCCCCCCEEECCCCCCCCEEEEEECCCCCHHHHCCCCCCEECCCCEECCCCC
YNMSDYFGHWLALGKKLEAAGAKLPRIYCVNWFRKDADGNFVWPGFGENMRVLSWMIDRV
CCHHHHHHHHHHHCCHHHHCCCCCCEEEEEEHEEECCCCCEECCCCCCCHHHHHHHHHHH
EGRGEGAEHVFGTSPRYTDLNWNGLAFTAEQFTQVTSIDRDAWQKELALHDELFTQLKHN
CCCCCCCCCCCCCCCCEECCCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LPQALAETKDALAKRLSA
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA