BacMap

Definition	Cupriavidus metallidurans CH34 chromosome, complete genome.
Accession	NC_007973
Length	3,928,089

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The map label for this gene is livF5 [H]

Identifier: 94312450

GI number: 94312450

Start: 3810485

End: 3811210

Strand: Reverse

Name: livF5 [H]

Synonym: Rmet_3519

Alternate gene names: 94312450

Gene position: 3811210-3810485 (Counterclockwise)

Preceding gene: 94312451

Following gene: 94312449

Centisome position: 97.02

GC content: 65.01

Gene sequence:

>726_bases
ATGGCGAACGACAACACTCCCGCGCTAGAGATCAAGGACCTGCACGCCTGGTACGGCGAGTCGCACATCCTGCATGGCGT
GGACCTGACCGTGAACCGCGGCGAAGTGGTCACGCTGCTGGGCCGTAACGGCGCCGGCCGCACCACGACCATGCGCGCGA
TCATGGGCCTGACCGGCGCACGCAAGGGATCGATCAAGGTCAACGGACACGAGACGATCGGCCTGCCCACGCACAAGATC
GCGCACTTCGGCATCGGCTACTGCCCGGAAGAGCGGGCGATCTTCTCGAGCCTGTCGTGTGAGGAAAACCTGCTGCTGCC
GCCGATGCTCAAGGGCGCCAACGCAGGCAAGGGCATGAGCGAGGCCGAGATCTACGAGATGTTCCCGAACCTCAAGGAGC
GGCGCCAGAGCCAGGGCACGCGCCTCTCGGGCGGCGAGCAGCAGATGTTGGCAGTGGGCCGCATCTTGCGCACTGGCGCG
AACCTGCTGCTGCTCGACGAGATTTCCGAAGGCCTCGCGCCGGTCATCGTGCAGGCGCTGGCCCGCATGATCCTGATGCT
CAAGAAGAAGGGCTACACGGTGGTGATGGTGGAGCAGAACTTCCGCTTCGCCGCGCCGCTGGCTGACCGCTTCTACGTGA
TGGAGCACGGCGCGATCGTCGAACGCTTCGCCGCGAACGAGCTGCAGGCCAAGATGCCCGTGCTCAACGAACTGCTTGGG
GTCTGA

Upstream 100 bases:

>100_bases
GGGCCCGTACGCGGAGGTGTCGAAGGACCCGCGCGTGATGGAAGCGTACATGGGCACGGCCGACGCCGAGCTGCAGGGCG
CGCATTAAGGGAGACGGACC

Downstream 100 bases:

>100_bases
TCGACGGCGCCGCGTCTGCGGTGCCGTGGTCGCATTTAACTCGCATTGGTGGCAGGAACGGGCGGCGTGACTGCGCTGCC
GCAACTCAGGAGACAACAAA

Product: leucine/isoleucine/valine ABC transporter ATP-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MANDNTPALEIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTMRAIMGLTGARKGSIKVNGHETIGLPTHKI
AHFGIGYCPEERAIFSSLSCEENLLLPPMLKGANAGKGMSEAEIYEMFPNLKERRQSQGTRLSGGEQQMLAVGRILRTGA
NLLLLDEISEGLAPVIVQALARMILMLKKKGYTVVMVEQNFRFAAPLADRFYVMEHGAIVERFAANELQAKMPVLNELLG
V

Sequences:

>Translated_241_residues
MANDNTPALEIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTMRAIMGLTGARKGSIKVNGHETIGLPTHKI
AHFGIGYCPEERAIFSSLSCEENLLLPPMLKGANAGKGMSEAEIYEMFPNLKERRQSQGTRLSGGEQQMLAVGRILRTGA
NLLLLDEISEGLAPVIVQALARMILMLKKKGYTVVMVEQNFRFAAPLADRFYVMEHGAIVERFAANELQAKMPVLNELLG
V
>Mature_240_residues
ANDNTPALEIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTMRAIMGLTGARKGSIKVNGHETIGLPTHKIA
HFGIGYCPEERAIFSSLSCEENLLLPPMLKGANAGKGMSEAEIYEMFPNLKERRQSQGTRLSGGEQQMLAVGRILRTGAN
LLLLDEISEGLAPVIVQALARMILMLKKKGYTVVMVEQNFRFAAPLADRFYVMEHGAIVERFAANELQAKMPVLNELLGV

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI45446740, Length=202, Percent_Identity=31.1881188118812, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI30795238, Length=204, Percent_Identity=29.4117647058824, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI47078218, Length=202, Percent_Identity=31.1881188118812, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI31657092, Length=205, Percent_Identity=26.3414634146341, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI27881501, Length=204, Percent_Identity=29.4117647058824, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI116734710, Length=193, Percent_Identity=29.5336787564767, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI21536376, Length=198, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI150417984, Length=196, Percent_Identity=29.5918367346939, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI9961252, Length=238, Percent_Identity=27.7310924369748, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI4505771, Length=238, Percent_Identity=27.7310924369748, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI9961250, Length=238, Percent_Identity=27.7310924369748, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI105990541, Length=191, Percent_Identity=27.2251308900524, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI27262624, Length=196, Percent_Identity=27.0408163265306, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI27262626, Length=196, Percent_Identity=27.0408163265306, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI42741659, Length=244, Percent_Identity=25.8196721311475, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI153792144, Length=211, Percent_Identity=26.0663507109005, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI27436953, Length=194, Percent_Identity=28.3505154639175, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI27477115, Length=219, Percent_Identity=28.310502283105, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI21536378, Length=221, Percent_Identity=23.0769230769231, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI9955963, Length=221, Percent_Identity=25.3393665158371, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI255708477, Length=240, Percent_Identity=27.0833333333333, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI148612844, Length=240, Percent_Identity=27.0833333333333, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI87082268, Length=235, Percent_Identity=40.8510638297872, Blast_Score=157, Evalue=4e-40,
Organism=Escherichia coli, GI1789593, Length=214, Percent_Identity=33.1775700934579, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1789672, Length=224, Percent_Identity=28.125, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1787029, Length=216, Percent_Identity=28.2407407407407, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1788225, Length=232, Percent_Identity=32.7586206896552, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787792, Length=241, Percent_Identity=30.7053941908714, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788897, Length=213, Percent_Identity=30.9859154929577, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787105, Length=248, Percent_Identity=27.4193548387097, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1790525, Length=222, Percent_Identity=27.9279279279279, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789864, Length=250, Percent_Identity=25.6, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI87081791, Length=219, Percent_Identity=28.7671232876712, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787089, Length=232, Percent_Identity=26.2931034482759, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788450, Length=221, Percent_Identity=28.0542986425339, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1788761, Length=229, Percent_Identity=27.5109170305677, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1788644, Length=235, Percent_Identity=29.7872340425532, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1786253, Length=237, Percent_Identity=29.957805907173, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787370, Length=215, Percent_Identity=26.5116279069767, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI48995001, Length=216, Percent_Identity=26.8518518518519, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787712, Length=218, Percent_Identity=28.4403669724771, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786703, Length=211, Percent_Identity=27.4881516587678, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786319, Length=229, Percent_Identity=27.5109170305677, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1786398, Length=223, Percent_Identity=24.6636771300448, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1790190, Length=222, Percent_Identity=27.027027027027, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787112, Length=238, Percent_Identity=29.8319327731092, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789991, Length=225, Percent_Identity=27.5555555555556, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI87081782, Length=227, Percent_Identity=27.7533039647577, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1786872, Length=217, Percent_Identity=28.110599078341, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1790467, Length=219, Percent_Identity=26.027397260274, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI48994943, Length=230, Percent_Identity=24.7826086956522, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87081709, Length=217, Percent_Identity=26.7281105990783, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788165, Length=217, Percent_Identity=26.7281105990783, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788210, Length=249, Percent_Identity=28.5140562248996, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1789873, Length=204, Percent_Identity=23.0392156862745, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1789891, Length=210, Percent_Identity=27.6190476190476, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87082267, Length=214, Percent_Identity=27.1028037383178, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1786698, Length=169, Percent_Identity=23.6686390532544, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789032, Length=261, Percent_Identity=23.7547892720307, Blast_Score=60, Evalue=9e-11,
Organism=Escherichia coli, GI48994883, Length=236, Percent_Identity=25, Blast_Score=60, Evalue=9e-11,
Organism=Escherichia coli, GI1786563, Length=224, Percent_Identity=25.4464285714286, Blast_Score=60, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=209, Percent_Identity=30.622009569378, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17565586, Length=220, Percent_Identity=28.1818181818182, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI115533608, Length=221, Percent_Identity=29.4117647058824, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71983523, Length=197, Percent_Identity=28.4263959390863, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71983516, Length=197, Percent_Identity=28.4263959390863, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71983510, Length=197, Percent_Identity=28.4263959390863, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25146777, Length=206, Percent_Identity=26.2135922330097, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI193211015, Length=242, Percent_Identity=26.4462809917355, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI193211017, Length=242, Percent_Identity=26.4462809917355, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71996809, Length=242, Percent_Identity=26.4462809917355, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71992285, Length=214, Percent_Identity=24.7663551401869, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71996550, Length=233, Percent_Identity=25.7510729613734, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17541710, Length=224, Percent_Identity=26.7857142857143, Blast_Score=64, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI212646699, Length=232, Percent_Identity=27.1551724137931, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24648314, Length=212, Percent_Identity=28.7735849056604, Blast_Score=103, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24666092, Length=216, Percent_Identity=28.7037037037037, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI221512771, Length=213, Percent_Identity=28.169014084507, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28574150, Length=200, Percent_Identity=28.5, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI116007328, Length=200, Percent_Identity=28.5, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24643674, Length=212, Percent_Identity=25.9433962264151, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28573573, Length=218, Percent_Identity=25.2293577981651, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28573571, Length=218, Percent_Identity=25.2293577981651, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI85725262, Length=209, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24650853, Length=203, Percent_Identity=25.1231527093596, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24650855, Length=203, Percent_Identity=25.1231527093596, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21357907, Length=219, Percent_Identity=28.310502283105, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281362751, Length=203, Percent_Identity=25.1231527093596, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24582014, Length=222, Percent_Identity=24.3243243243243, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582012, Length=222, Percent_Identity=24.3243243243243, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45550390, Length=219, Percent_Identity=24.6575342465753, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17136662, Length=213, Percent_Identity=26.2910798122066, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24649703, Length=215, Percent_Identity=26.5116279069767, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 26366; Mature: 26235

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDNTPALEIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTMRAIMGLTGA
CCCCCCCEEEEEHHHHHHCCCEEEECEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCC
RKGSIKVNGHETIGLPTHKIAHFGIGYCPEERAIFSSLSCEENLLLPPMLKGANAGKGMS
CCCCEEECCCEEECCCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
EAEIYEMFPNLKERRQSQGTRLSGGEQQMLAVGRILRTGANLLLLDEISEGLAPVIVQAL
HHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHCCHHHHHHHHH
ARMILMLKKKGYTVVMVEQNFRFAAPLADRFYVMEHGAIVERFAANELQAKMPVLNELLG
HHHHHHHHCCCCEEEEEECCCEEECCHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHC
V
C
>Mature Secondary Structure 
ANDNTPALEIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTMRAIMGLTGA
CCCCCCEEEEEHHHHHHCCCEEEECEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCC
RKGSIKVNGHETIGLPTHKIAHFGIGYCPEERAIFSSLSCEENLLLPPMLKGANAGKGMS
CCCCEEECCCEEECCCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
EAEIYEMFPNLKERRQSQGTRLSGGEQQMLAVGRILRTGANLLLLDEISEGLAPVIVQAL
HHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHCCHHHHHHHHH
ARMILMLKKKGYTVVMVEQNFRFAAPLADRFYVMEHGAIVERFAANELQAKMPVLNELLG
HHHHHHHHCCCCEEEEEECCCEEECCHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]