Click here to switch to the map view.

The map label for this gene is hmp [H]

Identifier: 94312313

GI number: 94312313

Start: 3657147

End: 3658436

Strand: Reverse

Name: hmp [H]

Synonym: Rmet_3382

Alternate gene names: 94312313

Gene position: 3658436-3657147 (Counterclockwise)

Preceding gene: 94312314

Following gene: 94312312

Centisome position: 93.14

GC content: 63.88

Gene sequence:

>1290_bases
ATGTCGTCTGCCGGCTTTATTGTGTGCAAAAACATGCATTTTACATGCATGATTCAAGGAGTTTTGCTGATGTTGTCCGC
TGCTTCCCGCCCCTATATCGATGCCAGCGTCCCCGTGCTGCGTGAGCACGGTCTTGCGATCACGACACATTTCTACCGAG
AGATGTTCGCGGCGCGCCCCGAACTGAAGCGCATTTTCAACATGGGCAATCAGGCCAATGGCTCGCAGCAGCAATCGCTG
GCCTCGGCAGTGTTCGCCTATGCGGCCAACATCGATCGTGCCGACGTGCTTGCGCCCGTGGTCGAGCGCATCGTCCACAA
GCACGTGGCGGTCGGATTGACGCCGGCTCACTATCCGATCGTCGGCAAGTATCTGCTGGAAGCCATTGCCGCCGTGCTGG
GCGACGCCGCCACGCCGCCGCTGCTGGCCGCCTGGGACGAAGCCTACTGGCTGCTGGCCGGCGAACTGATTGCCGCAGAG
GCCCGACTCTATGAACGCCATGGCGTGGCGGCAGGTGAGCTTCTGCGCGTGCGCGTGGTGAACCGCGAGCAGGAGGGGGA
TCAGGTGGTGGCGTTGACGCTGGCCGCCGAAGACGGCTCGCCGCTGCGTGACTTCGCGCCGGGTCAGTACATCAGCGTCG
AGGCATCGTTCGCCGACGGACGCCGTCAACTGCGCCAGTATTCGCTTTCGGGCGAGCGCGGCCTCCCGACGTGGCGTATC
TCGGTGAAGCGCGAAGATGGCGCAAGCGACGCGCCGGCGGGTACGGTATCGAACTGGCTGCATGAGAACGCCCAGGTCGG
CACGACGCTGCACGTGAGCACCCCTTGGGGCGATTTCGCGCCCGAGCTCGATAGCCGCCAGCCGATCGTGCTGATGTCGG
CAGGCATCGGCGTGACGCCGATGGTCTCGGTGTTGCGCACGCTCGCACACGAAAATCCGCATCGACCGGTGCTGTTCGCC
CATGCCGCCCGTCATGGTCGTCAGCACGCGCATCGTCGCGACCTGGCATGGGCGCGCGAGCGGATGCCCAATCTGCGTAC
CCATGTCAGCTATGAAACGCCGCAGTCGGTGGATGCCTCGGGGCGCGACTACGACCACGCCGGCACGATGCCGGTCGAGG
CCCTGCTCAAGTCTGGTGACAACGCCGCTTTCAATGACGCCCGCTTCTATCTGTGTGGCCCGCTTGGCTTCATGCAGGAA
CAGCGTCGCGCGTTGATCGCCTCGGGCGTTCCAGTCGGACATATTCATCGCGAAGTGTTCGGGCCGGACCTGCTCGACGA
CATCCTTTGA

Upstream 100 bases:

>100_bases
CGATGTGTCAGGTAGCCGCACGGCCGAATCGATCATCAGCCTGCATCGAGATTTCCTGAGCCGGCACGTGGCATAAGGAT
TCGGGGGGCCACCCCGACCC

Downstream 100 bases:

>100_bases
TCGCCGTCGCATTCTTCGAATCTGCCTGCTGATATTGCGTCAGTTGGCAGACGACAGGTGACATGTGGGTCTGGTACGCT
AGGCGGTCTCCCTATTCGAG

Product: Flavohemoprotein

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSSAGFIVCKNMHFTCMIQGVLLMLSAASRPYIDASVPVLREHGLAITTHFYREMFAARPELKRIFNMGNQANGSQQQSL
ASAVFAYAANIDRADVLAPVVERIVHKHVAVGLTPAHYPIVGKYLLEAIAAVLGDAATPPLLAAWDEAYWLLAGELIAAE
ARLYERHGVAAGELLRVRVVNREQEGDQVVALTLAAEDGSPLRDFAPGQYISVEASFADGRRQLRQYSLSGERGLPTWRI
SVKREDGASDAPAGTVSNWLHENAQVGTTLHVSTPWGDFAPELDSRQPIVLMSAGIGVTPMVSVLRTLAHENPHRPVLFA
HAARHGRQHAHRRDLAWARERMPNLRTHVSYETPQSVDASGRDYDHAGTMPVEALLKSGDNAAFNDARFYLCGPLGFMQE
QRRALIASGVPVGHIHREVFGPDLLDDIL

Sequences:

>Translated_429_residues
MSSAGFIVCKNMHFTCMIQGVLLMLSAASRPYIDASVPVLREHGLAITTHFYREMFAARPELKRIFNMGNQANGSQQQSL
ASAVFAYAANIDRADVLAPVVERIVHKHVAVGLTPAHYPIVGKYLLEAIAAVLGDAATPPLLAAWDEAYWLLAGELIAAE
ARLYERHGVAAGELLRVRVVNREQEGDQVVALTLAAEDGSPLRDFAPGQYISVEASFADGRRQLRQYSLSGERGLPTWRI
SVKREDGASDAPAGTVSNWLHENAQVGTTLHVSTPWGDFAPELDSRQPIVLMSAGIGVTPMVSVLRTLAHENPHRPVLFA
HAARHGRQHAHRRDLAWARERMPNLRTHVSYETPQSVDASGRDYDHAGTMPVEALLKSGDNAAFNDARFYLCGPLGFMQE
QRRALIASGVPVGHIHREVFGPDLLDDIL
>Mature_428_residues
SSAGFIVCKNMHFTCMIQGVLLMLSAASRPYIDASVPVLREHGLAITTHFYREMFAARPELKRIFNMGNQANGSQQQSLA
SAVFAYAANIDRADVLAPVVERIVHKHVAVGLTPAHYPIVGKYLLEAIAAVLGDAATPPLLAAWDEAYWLLAGELIAAEA
RLYERHGVAAGELLRVRVVNREQEGDQVVALTLAAEDGSPLRDFAPGQYISVEASFADGRRQLRQYSLSGERGLPTWRIS
VKREDGASDAPAGTVSNWLHENAQVGTTLHVSTPWGDFAPELDSRQPIVLMSAGIGVTPMVSVLRTLAHENPHRPVLFAH
AARHGRQHAHRRDLAWARERMPNLRTHVSYETPQSVDASGRDYDHAGTMPVEALLKSGDNAAFNDARFYLCGPLGFMQEQ
RRALIASGVPVGHIHREVFGPDLLDDIL

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=408, Percent_Identity=37.7450980392157, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1787658, Length=251, Percent_Identity=27.8884462151394, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788104, Length=140, Percent_Identity=30, Blast_Score=62, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6321673, Length=418, Percent_Identity=35.6459330143541, Blast_Score=210, Evalue=4e-55,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 46975; Mature: 46844

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAGFIVCKNMHFTCMIQGVLLMLSAASRPYIDASVPVLREHGLAITTHFYREMFAARP
CCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCEEHHHHHHHHHHCCH
ELKRIFNMGNQANGSQQQSLASAVFAYAANIDRADVLAPVVERIVHKHVAVGLTPAHYPI
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHH
VGKYLLEAIAAVLGDAATPPLLAAWDEAYWLLAGELIAAEARLYERHGVAAGELLRVRVV
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEE
NREQEGDQVVALTLAAEDGSPLRDFAPGQYISVEASFADGRRQLRQYSLSGERGLPTWRI
CCCCCCCEEEEEEEEECCCCCHHHCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCCCEEEE
SVKREDGASDAPAGTVSNWLHENAQVGTTLHVSTPWGDFAPELDSRQPIVLMSAGIGVTP
EEEECCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCHHH
MVSVLRTLAHENPHRPVLFAHAARHGRQHAHRRDLAWARERMPNLRTHVSYETPQSVDAS
HHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHEECCCCCCCCCCC
GRDYDHAGTMPVEALLKSGDNAAFNDARFYLCGPLGFMQEQRRALIASGVPVGHIHREVF
CCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHC
GPDLLDDIL
CCHHHHHCC
>Mature Secondary Structure 
SSAGFIVCKNMHFTCMIQGVLLMLSAASRPYIDASVPVLREHGLAITTHFYREMFAARP
CCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCEEHHHHHHHHHHCCH
ELKRIFNMGNQANGSQQQSLASAVFAYAANIDRADVLAPVVERIVHKHVAVGLTPAHYPI
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHH
VGKYLLEAIAAVLGDAATPPLLAAWDEAYWLLAGELIAAEARLYERHGVAAGELLRVRVV
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEE
NREQEGDQVVALTLAAEDGSPLRDFAPGQYISVEASFADGRRQLRQYSLSGERGLPTWRI
CCCCCCCEEEEEEEEECCCCCHHHCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCCCEEEE
SVKREDGASDAPAGTVSNWLHENAQVGTTLHVSTPWGDFAPELDSRQPIVLMSAGIGVTP
EEEECCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCHHH
MVSVLRTLAHENPHRPVLFAHAARHGRQHAHRRDLAWARERMPNLRTHVSYETPQSVDAS
HHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHEECCCCCCCCCCC
GRDYDHAGTMPVEALLKSGDNAAFNDARFYLCGPLGFMQEQRRALIASGVPVGHIHREVF
CCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHC
GPDLLDDIL
CCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14521231; 11481432 [H]