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The map label for this gene is nirF [H]

Identifier: 94312262

GI number: 94312262

Start: 3595981

End: 3597540

Strand: Reverse

Name: nirF [H]

Synonym: Rmet_3331

Alternate gene names: 94312262

Gene position: 3597540-3595981 (Counterclockwise)

Preceding gene: 94312263

Following gene: 94312261

Centisome position: 91.58

GC content: 59.62

Gene sequence:

>1560_bases
ATGTTCGGAAGAACGATGTTGTTTGTCACGTTGACCGGTGTGTCGTCGGCCATCTCGGCCATGACCGCCGAGGAGATAAA
GCGCGCCGACGCGCTCTATCAGGCCAATTGTGCAGTGTGTCACTCGGCAAGCCGGGGCGGGTATGTGGGGCCCGCCTTGC
ACAAGGACCGCCTTGTGCAGAGCGAGGCTGCGTTGCGTGGCACGATCATGACCGGTATCCCCGATACGCTGATGCCACCA
TTCCACTCGCGCCTGCCCGATGCAGATATTCATTTGCTGGCGGAGTACATCCATTCGCAGCCGGTCAGCAAGCCGGAATG
GTCGATGAAGCAGGCCCAGGACAGCCTGGAAGTGCTGATCGACGAGAAGACGCTCCCGTCGAAGCCGACTTACAAGATCG
ACTCGGTCTACGACCTGATGGTGGTCATGGCCCGTGGACGCTATGGCCGGGGAATGGGTTCCGATAACGCCAAGACGGTC
TTCCTCGACGGCAAAACCAATCAGGTGGTTGGCGAGATTGCCACACCGATTGCGCCGCACATCATGGATTTCAGCCCGGT
CAATGAGCGATGGGCGTGGCTCAAGAGCGACGATGGCTACGTCTACAAGATCGACCTTTACTCGCTCAAGGTTGTACGCA
AGATCAAGACGGGTTTGACGGGGCCGGGCATCGCCGTGTCGAACGACGGCAAATGGCTTGCCGCCAGTTCATTCGTGCCA
AACCTCGTCATGATCCTGAAGGCGGATACGCTCGAACCCGTGAAGCACCTTCGGCTGGAAGGCACGGACCCGGACGGCAA
GTTCATTCAGTCGGCAGGCGGGCCGGTAATCGGCAGCCGGATTAACAACAAGTTCGCGCTGACTCTGCGCCAGGCGGGGC
AGATCTGGATCGTGGACCCGGATCGAGAGGGGATGCCCGTCACCAAGCTCGAGAAGGTCGGACGCAAGTTGCACGATACG
TTCCTGACGCCAGATGGGCGATATTCGATGGTGGCCTCGTATGACGACAACAAGATCGTCGCCATCGATCTCAAGGAAGA
CAAGGTGGTTCGCGATATTCCAGCGGGCTGCCAGCCGCACGTCGGATCTGGCGCGGTGACGCCGATCGGCGGCCGAATGC
TGGCTTTTGGTACCAACATCGGCAAGTGCGACAAGGGCGATGTGGTGACAGTCTGGGACACCAAGGATTTCTCGGTTGTG
AAGAACATCCGCGTGGCCGGCCCGACCGAATCGCCGGCAGCCCATCCTGATGCGCCGTATGTCGCGGTCGATATCGTCAG
CAAGGACAAGAGGGCCTCACAGATCCAGCTTATTGACAAGCAGAAGCTTGAAGTCGTGCGCACGCTCGAAGCCGGTGGCC
ATGCGTTCTTCCCGGAGTACACGCGTGATGGCAAGTATCTGTACGTGAGCGCGGGCTACTACGGAGACCGGATTCGTATC
TACGACAGCAAGACACTGGAGTTGGTCGCCGAACACAAGATGGAAAGCCCGGCAGGCATCTTCTCGCGTGCCCGGACGCA
ATGGATCACCGTGGGCCTGCCGCCCACTCGCAAGAACTGA

Upstream 100 bases:

>100_bases
ACCCATCACCTTTCAGTTTCCATCCCCTGCGCGCCGTCACTCGACGGAACAGACTTCAATTGTTGCTGCGCGATTGCGTG
GGGTACTTGGAGTCTGGTCC

Downstream 100 bases:

>100_bases
TGGAGGAGTGACGCGATGACCGATTTTCGTTTGAACCGTAGCGCCGTCACGCTTGCATTGGTGCTGTTCTCGGTAGGAGT
GCACGCCGAGACACCGAAGC

Product: cytochrome c, class I:Cytochrome d1, heme region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MFGRTMLFVTLTGVSSAISAMTAEEIKRADALYQANCAVCHSASRGGYVGPALHKDRLVQSEAALRGTIMTGIPDTLMPP
FHSRLPDADIHLLAEYIHSQPVSKPEWSMKQAQDSLEVLIDEKTLPSKPTYKIDSVYDLMVVMARGRYGRGMGSDNAKTV
FLDGKTNQVVGEIATPIAPHIMDFSPVNERWAWLKSDDGYVYKIDLYSLKVVRKIKTGLTGPGIAVSNDGKWLAASSFVP
NLVMILKADTLEPVKHLRLEGTDPDGKFIQSAGGPVIGSRINNKFALTLRQAGQIWIVDPDREGMPVTKLEKVGRKLHDT
FLTPDGRYSMVASYDDNKIVAIDLKEDKVVRDIPAGCQPHVGSGAVTPIGGRMLAFGTNIGKCDKGDVVTVWDTKDFSVV
KNIRVAGPTESPAAHPDAPYVAVDIVSKDKRASQIQLIDKQKLEVVRTLEAGGHAFFPEYTRDGKYLYVSAGYYGDRIRI
YDSKTLELVAEHKMESPAGIFSRARTQWITVGLPPTRKN

Sequences:

>Translated_519_residues
MFGRTMLFVTLTGVSSAISAMTAEEIKRADALYQANCAVCHSASRGGYVGPALHKDRLVQSEAALRGTIMTGIPDTLMPP
FHSRLPDADIHLLAEYIHSQPVSKPEWSMKQAQDSLEVLIDEKTLPSKPTYKIDSVYDLMVVMARGRYGRGMGSDNAKTV
FLDGKTNQVVGEIATPIAPHIMDFSPVNERWAWLKSDDGYVYKIDLYSLKVVRKIKTGLTGPGIAVSNDGKWLAASSFVP
NLVMILKADTLEPVKHLRLEGTDPDGKFIQSAGGPVIGSRINNKFALTLRQAGQIWIVDPDREGMPVTKLEKVGRKLHDT
FLTPDGRYSMVASYDDNKIVAIDLKEDKVVRDIPAGCQPHVGSGAVTPIGGRMLAFGTNIGKCDKGDVVTVWDTKDFSVV
KNIRVAGPTESPAAHPDAPYVAVDIVSKDKRASQIQLIDKQKLEVVRTLEAGGHAFFPEYTRDGKYLYVSAGYYGDRIRI
YDSKTLELVAEHKMESPAGIFSRARTQWITVGLPPTRKN
>Mature_519_residues
MFGRTMLFVTLTGVSSAISAMTAEEIKRADALYQANCAVCHSASRGGYVGPALHKDRLVQSEAALRGTIMTGIPDTLMPP
FHSRLPDADIHLLAEYIHSQPVSKPEWSMKQAQDSLEVLIDEKTLPSKPTYKIDSVYDLMVVMARGRYGRGMGSDNAKTV
FLDGKTNQVVGEIATPIAPHIMDFSPVNERWAWLKSDDGYVYKIDLYSLKVVRKIKTGLTGPGIAVSNDGKWLAASSFVP
NLVMILKADTLEPVKHLRLEGTDPDGKFIQSAGGPVIGSRINNKFALTLRQAGQIWIVDPDREGMPVTKLEKVGRKLHDT
FLTPDGRYSMVASYDDNKIVAIDLKEDKVVRDIPAGCQPHVGSGAVTPIGGRMLAFGTNIGKCDKGDVVTVWDTKDFSVV
KNIRVAGPTESPAAHPDAPYVAVDIVSKDKRASQIQLIDKQKLEVVRTLEAGGHAFFPEYTRDGKYLYVSAGYYGDRIRI
YDSKTLELVAEHKMESPAGIFSRARTQWITVGLPPTRKN

Specific function: Required for the biosynthesis of heme d1 of nitrite reductase. Could have a dehydrogenase activity yielding sirohydrochlorin from precorrin-2 or dehydrogenation of propionate side chain C17 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011048
- InterPro:   IPR003143 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 56961; Mature: 56961

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS51007 CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGRTMLFVTLTGVSSAISAMTAEEIKRADALYQANCAVCHSASRGGYVGPALHKDRLVQ
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHH
SEAALRGTIMTGIPDTLMPPFHSRLPDADIHLLAEYIHSQPVSKPEWSMKQAQDSLEVLI
HHHHHCCEEECCCCHHCCCCHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEE
DEKTLPSKPTYKIDSVYDLMVVMARGRYGRGMGSDNAKTVFLDGKTNQVVGEIATPIAPH
ECCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHCCCHHH
IMDFSPVNERWAWLKSDDGYVYKIDLYSLKVVRKIKTGLTGPGIAVSNDGKWLAASSFVP
HCCCCCCCCCEEEEECCCCEEEEEEHHHHHHHHHHHHCCCCCCEEECCCCCEEEECCCCC
NLVMILKADTLEPVKHLRLEGTDPDGKFIQSAGGPVIGSRINNKFALTLRQAGQIWIVDP
CEEEEEECCCCCCHHHEEECCCCCCCHHHHCCCCCEEECCCCCEEEEEEECCCEEEEECC
DREGMPVTKLEKVGRKLHDTFLTPDGRYSMVASYDDNKIVAIDLKEDKVVRDIPAGCQPH
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEECCCCCHHHHCCCCCCCC
VGSGAVTPIGGRMLAFGTNIGKCDKGDVVTVWDTKDFSVVKNIRVAGPTESPAAHPDAPY
CCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCHHHHEEEEECCCCCCCCCCCCCE
VAVDIVSKDKRASQIQLIDKQKLEVVRTLEAGGHAFFPEYTRDGKYLYVSAGYYGDRIRI
EEEEEECCCCCCCCEEEHHHHHHHHHHHHHCCCCEECCCCCCCCCEEEEECCCCCCEEEE
YDSKTLELVAEHKMESPAGIFSRARTQWITVGLPPTRKN
ECCCHHHHHHHHCCCCCHHHHHHCCEEEEEEECCCCCCC
>Mature Secondary Structure
MFGRTMLFVTLTGVSSAISAMTAEEIKRADALYQANCAVCHSASRGGYVGPALHKDRLVQ
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHH
SEAALRGTIMTGIPDTLMPPFHSRLPDADIHLLAEYIHSQPVSKPEWSMKQAQDSLEVLI
HHHHHCCEEECCCCHHCCCCHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEE
DEKTLPSKPTYKIDSVYDLMVVMARGRYGRGMGSDNAKTVFLDGKTNQVVGEIATPIAPH
ECCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHCCCHHH
IMDFSPVNERWAWLKSDDGYVYKIDLYSLKVVRKIKTGLTGPGIAVSNDGKWLAASSFVP
HCCCCCCCCCEEEEECCCCEEEEEEHHHHHHHHHHHHCCCCCCEEECCCCCEEEECCCCC
NLVMILKADTLEPVKHLRLEGTDPDGKFIQSAGGPVIGSRINNKFALTLRQAGQIWIVDP
CEEEEEECCCCCCHHHEEECCCCCCCHHHHCCCCCEEECCCCCEEEEEEECCCEEEEECC
DREGMPVTKLEKVGRKLHDTFLTPDGRYSMVASYDDNKIVAIDLKEDKVVRDIPAGCQPH
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEECCCCCHHHHCCCCCCCC
VGSGAVTPIGGRMLAFGTNIGKCDKGDVVTVWDTKDFSVVKNIRVAGPTESPAAHPDAPY
CCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCHHHHEEEEECCCCCCCCCCCCCE
VAVDIVSKDKRASQIQLIDKQKLEVVRTLEAGGHAFFPEYTRDGKYLYVSAGYYGDRIRI
EEEEEECCCCCCCCEEEHHHHHHHHHHHHHCCCCEECCCCCCCCCEEEEECCCCCCEEEE
YDSKTLELVAEHKMESPAGIFSRARTQWITVGLPPTRKN
ECCCHHHHHHHHCCCCCHHHHHHCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8566817; 10984043 [H]