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The map label for this gene is b2999 [C]

Identifier: 94312252

GI number: 94312252

Start: 3584125

End: 3585354

Strand: Reverse

Name: b2999 [C]

Synonym: Rmet_3321

Alternate gene names: 94312252

Gene position: 3585354-3584125 (Counterclockwise)

Preceding gene: 94312254

Following gene: 94312251

Centisome position: 91.27

GC content: 64.39

Gene sequence:

>1230_bases
ATGGGCCAGACTATCCAGATCCAGACCCAGGACGGCAACTTCAGTGGCTATCTCGCCACTCCGGCAGGAGGCAAGGGCCC
TGGCATCGTGCTGTGCCAGGAGATCTTCGGCGTCAATGCCACCATGCGGCAGGTGGCCGACTACTATGCCGAGGAAGGCT
ATACCGTGCTGGTGCCGGATCTGTTCTGGCGCATCGAGCACGGGATCGAGCTATCCGACCGTGGGGCGGACTTCCAGCGT
GCACTCGGCTTGTACCAGCAATTTGACGAAGATAAGGGCGTGCAGGACGTCGGCGCCGCTCTCGCCACGCTACGCCAACG
CCCCGAATGCGCTGGCCAGACCGGTGTGCTCGGCTTCTGCCTCGGCGGCAAGCTGGCCTATCTGGCGGCGTGCCGACTGC
CGGACGTAGCCTGTGCCGTCGGCTACTACGGGGTTGGTATCGAGCGCGCGCTTGGCGAGGCCAGAAACATCCGTGGCCGG
CTGGTACTGCATGTCGCCGAGCGCGATGGCTTCTGCCCGCCCGAGGCGCAGGCGAGCATCCGCGAAGCGCTGTCCGGCCA
ACCCGGTGTCGAACTGTACGTCTATCCGGGGATGGATCACGCGTTCGCGCGCACCGGCGGCGAGCACTTCGACAAGGCCT
CGGCCCTGATGGCGCACCAGCGTTCGATCGCGGCGTTCCGCGCCGAGATGGGCCCGCACTACGATTTCTCCGCGCTGTGG
GATAAGCATTGCGAGTACGAATTCGCCACGCGCGATGTCGATGCCACGATGGCGACGATGGTGGCCGAGCCATACGTCAA
CCACATCCCGACGATGACCGGTGGCGTGGGCTACCGGCAGCTCAAGCGCTTCTACCAGCACCACTTCGTCCACAGCAATC
CGCCCGACACCACGCTGATTCCGCTGTCCCGCACGATCGGCGCCAGCCAGGTAGTGGACGAGATCCTCTTCTGCTTTACC
CACACCTGCGAGATCGACTGGATGCTCCCCGGCGTGCCTCCAACCGGCAAGCGCGTAGAGATCCCGCTGATCGCCATCAT
CAAGTTCCGTGGCGACAAGCTCTATCACGAGCATATCTACTGGGACCAGGCCAGCGTGCTGGTACAGGTCGGCCTGCTCG
ATCCCAAGGGGCTACCCGTGGCCGGAGTCGAGACGGCACGCAAGCTGCTGGACGAAACCCTGCCATCCAACACGCTGATG
GCGCGCTGGAGCGAGAGCGAAGGCAAGTAA

Upstream 100 bases:

>100_bases
ACTAGGTGGCTAGTTTGCTGCGGTGCGGGTCTCCGATCATGAAGGCAACGGCGGGGCACGCACGGCGGCCATACCCACAA
AACATACAGGAGCAAAAGGC

Downstream 100 bases:

>100_bases
CGCCCCCGGCCCACGTTGCGGAACGGGCGGAACGCGGGGACGGCACGTCAGAGGCCGCCCCGCACATCAACCGAGGAGAC
ACCATCATGCAAGGCTTGCA

Product: dienelactone hydrolase

Products: NA

Alternate protein names: Dienelactone hydrolase; DLH [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MGQTIQIQTQDGNFSGYLATPAGGKGPGIVLCQEIFGVNATMRQVADYYAEEGYTVLVPDLFWRIEHGIELSDRGADFQR
ALGLYQQFDEDKGVQDVGAALATLRQRPECAGQTGVLGFCLGGKLAYLAACRLPDVACAVGYYGVGIERALGEARNIRGR
LVLHVAERDGFCPPEAQASIREALSGQPGVELYVYPGMDHAFARTGGEHFDKASALMAHQRSIAAFRAEMGPHYDFSALW
DKHCEYEFATRDVDATMATMVAEPYVNHIPTMTGGVGYRQLKRFYQHHFVHSNPPDTTLIPLSRTIGASQVVDEILFCFT
HTCEIDWMLPGVPPTGKRVEIPLIAIIKFRGDKLYHEHIYWDQASVLVQVGLLDPKGLPVAGVETARKLLDETLPSNTLM
ARWSESEGK

Sequences:

>Translated_409_residues
MGQTIQIQTQDGNFSGYLATPAGGKGPGIVLCQEIFGVNATMRQVADYYAEEGYTVLVPDLFWRIEHGIELSDRGADFQR
ALGLYQQFDEDKGVQDVGAALATLRQRPECAGQTGVLGFCLGGKLAYLAACRLPDVACAVGYYGVGIERALGEARNIRGR
LVLHVAERDGFCPPEAQASIREALSGQPGVELYVYPGMDHAFARTGGEHFDKASALMAHQRSIAAFRAEMGPHYDFSALW
DKHCEYEFATRDVDATMATMVAEPYVNHIPTMTGGVGYRQLKRFYQHHFVHSNPPDTTLIPLSRTIGASQVVDEILFCFT
HTCEIDWMLPGVPPTGKRVEIPLIAIIKFRGDKLYHEHIYWDQASVLVQVGLLDPKGLPVAGVETARKLLDETLPSNTLM
ARWSESEGK
>Mature_408_residues
GQTIQIQTQDGNFSGYLATPAGGKGPGIVLCQEIFGVNATMRQVADYYAEEGYTVLVPDLFWRIEHGIELSDRGADFQRA
LGLYQQFDEDKGVQDVGAALATLRQRPECAGQTGVLGFCLGGKLAYLAACRLPDVACAVGYYGVGIERALGEARNIRGRL
VLHVAERDGFCPPEAQASIREALSGQPGVELYVYPGMDHAFARTGGEHFDKASALMAHQRSIAAFRAEMGPHYDFSALWD
KHCEYEFATRDVDATMATMVAEPYVNHIPTMTGGVGYRQLKRFYQHHFVHSNPPDTTLIPLSRTIGASQVVDEILFCFTH
TCEIDWMLPGVPPTGKRVEIPLIAIIKFRGDKLYHEHIYWDQASVLVQVGLLDPKGLPVAGVETARKLLDETLPSNTLMA
RWSESEGK

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI48994982, Length=232, Percent_Identity=25.4310344827586, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: =3.1.1.45 [H]

Molecular weight: Translated: 45026; Mature: 44895

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQTIQIQTQDGNFSGYLATPAGGKGPGIVLCQEIFGVNATMRQVADYYAEEGYTVLVPD
CCCEEEEEECCCCEEEEEECCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHCCCEEEECH
LFWRIEHGIELSDRGADFQRALGLYQQFDEDKGVQDVGAALATLRQRPECAGQTGVLGFC
HHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEE
LGGKLAYLAACRLPDVACAVGYYGVGIERALGEARNIRGRLVLHVAERDGFCPPEAQASI
CCCHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHH
REALSGQPGVELYVYPGMDHAFARTGGEHFDKASALMAHQRSIAAFRAEMGPHYDFSALW
HHHHCCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
DKHCEYEFATRDVDATMATMVAEPYVNHIPTMTGGVGYRQLKRFYQHHFVHSNPPDTTLI
HHCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEE
PLSRTIGASQVVDEILFCFTHTCEIDWMLPGVPPTGKRVEIPLIAIIKFRGDKLYHEHIY
ECHHHCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEEEEEEEECCCEEEEHHEE
WDQASVLVQVGLLDPKGLPVAGVETARKLLDETLPSNTLMARWSESEGK
ECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCC
>Mature Secondary Structure 
GQTIQIQTQDGNFSGYLATPAGGKGPGIVLCQEIFGVNATMRQVADYYAEEGYTVLVPD
CCEEEEEECCCCEEEEEECCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHCCCEEEECH
LFWRIEHGIELSDRGADFQRALGLYQQFDEDKGVQDVGAALATLRQRPECAGQTGVLGFC
HHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEE
LGGKLAYLAACRLPDVACAVGYYGVGIERALGEARNIRGRLVLHVAERDGFCPPEAQASI
CCCHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHH
REALSGQPGVELYVYPGMDHAFARTGGEHFDKASALMAHQRSIAAFRAEMGPHYDFSALW
HHHHCCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
DKHCEYEFATRDVDATMATMVAEPYVNHIPTMTGGVGYRQLKRFYQHHFVHSNPPDTTLI
HHCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEE
PLSRTIGASQVVDEILFCFTHTCEIDWMLPGVPPTGKRVEIPLIAIIKFRGDKLYHEHIY
ECHHHCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEEEEEEEECCCEEEEHHEE
WDQASVLVQVGLLDPKGLPVAGVETARKLLDETLPSNTLMARWSESEGK
ECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8138139 [H]