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The map label for this gene is gltD [H]

Identifier: 94312193

GI number: 94312193

Start: 3531973

End: 3533436

Strand: Reverse

Name: gltD [H]

Synonym: Rmet_3262

Alternate gene names: 94312193

Gene position: 3533436-3531973 (Counterclockwise)

Preceding gene: 94312194

Following gene: 94312191

Centisome position: 89.95

GC content: 64.96

Gene sequence:

>1464_bases
ATGGGTAAGGCGACTGGTTTTCTCGAATTTGCGCGCCAGAATGAAGGCTACGAACCCGTAGTCAAGCGCGTGAAGCACTA
CAAGGAATTCGTGTTCGCGCTGTCGGACAGCGAGGCGAAGATTCAGGGTGCGCGCTGCATGGACTGCGGTATCCCGTTCT
GCAACAACGGCTGCCCGGTCAACAACATCATTCCGGACTTCAACGACCTCGTGTATCGCCAGGACTGGAAGTCGGCGATC
GAAGTCCTGCACCAGACCAACAACTTCCCCGAGTTCACGGGCCGCATCTGCCCGGCGCCGTGCGAAGCCGCCTGCACGCT
CGGCATCAACGAGCTGCCGGTTGGCATCAAGTCGATCGAGCACGCGATCATCGACAAGGCCTGGGAAGAGGGCTGGGTCC
AGCCGCAGGTGCCTGCGCACAAGACCGGCAAGACCGTCGCTGTCGTGGGTTCGGGACCCGCCGGCATGGCAGCCGCCCAG
CAACTGGCGCGCGCCGGCCACGCTGTGACCGTGTTCGAGAAGAATGACCGCATCGGCGGCCTGCTCCGCTACGGCATTCC
CGACTTCAAGATGGAGAAGACCCTGATCGACCGCCGCATCGAGCAGATGCAGGCCGAAGGCGTGACCTTCCGCGCTGGCG
TGATGGTGACCGACGGCGCGCTGCCGGCCGGCATCAAGAACTACGCTCGCGAGACGATCTCGGCCCAGGCGCTGATGGAG
CAGTTCGACGCCGTGGTACTGGCCGGTGGCGCCGAAGTGCCGCGCGACCTGCCCGTGCCGGGCCGCGATCTGGCCGGCGT
GCACTACGCGCTGGAGTTCCTGATCCCGCAGAACAAGGAAGTGGCGGGCGATGGCGTCAACGAGATTCGTGCCGAAGGCA
AGCACGTCATCGTGATCGGCGGTGGCGATACGGGTTCGGACTGCGTGGGCACGTCGAACCGCCACGGCGCAACCTCGGTG
ACGCAGTTCGAACTGCTGCCGCAGCCGCCGGAAGAAGAGAACAAGCCGCTGGTGTGGCCGTACTGGCCGATCAAGCTGCG
CACGTCGTCGTCGCACGACGAAGGCTGCTCGCGTGACTGGTCGGTGGCCACCAAGGAACTGGTTGGCGAGAACGGCAAGG
TCAAGGCCCTGAAGGCCTGCCGCGTCGAATGGAAGGACGGCAAGATGCAGGAAGTGCCGGGCAGCGAGTTCGTGCTGCAG
GCCGACCTGGTGCTGTTGGCCATGGGCTTCACGAACCCGGTCGGCTCGATGCTCGAAGCGTTCGGTGTCGACACGGATGC
ACGCAAGAACGCCAAGGCATCGACCGAAGGCGATCGCGCTTACTACACCAACGTGCCGAAGGTGTTCGCCGCTGGCGACG
TGCGCCGTGGTCAGTCGCTCGTGGTGTGGGCCATCCGCGAAGGCCGCCAGGCTGCCCGCTCGGTGGACGCGTTCCTGATG
GGCCATTCGACCCTGCCGCGCTGA

Upstream 100 bases:

>100_bases
ACGAGATGGCGCCGCGGCCAGGTTCGCGGCGCCGACAAGATACTGACCCCACGCGAGGCCGGCCCGGCCGCCTCGCGCCA
AGCAAGACCAAGGATGCAAC

Downstream 100 bases:

>100_bases
TCCGTAGCGTTGGGTTCAAAAGCAAAACCCCCCGCAAGTGCGAACTTGCGGGGGGTTTGTTTATGGCTGGGTTGAGAGTC
GTGGCGGGGCGTTCTAAAGC

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MGKATGFLEFARQNEGYEPVVKRVKHYKEFVFALSDSEAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWKSAI
EVLHQTNNFPEFTGRICPAPCEAACTLGINELPVGIKSIEHAIIDKAWEEGWVQPQVPAHKTGKTVAVVGSGPAGMAAAQ
QLARAGHAVTVFEKNDRIGGLLRYGIPDFKMEKTLIDRRIEQMQAEGVTFRAGVMVTDGALPAGIKNYARETISAQALME
QFDAVVLAGGAEVPRDLPVPGRDLAGVHYALEFLIPQNKEVAGDGVNEIRAEGKHVIVIGGGDTGSDCVGTSNRHGATSV
TQFELLPQPPEEENKPLVWPYWPIKLRTSSSHDEGCSRDWSVATKELVGENGKVKALKACRVEWKDGKMQEVPGSEFVLQ
ADLVLLAMGFTNPVGSMLEAFGVDTDARKNAKASTEGDRAYYTNVPKVFAAGDVRRGQSLVVWAIREGRQAARSVDAFLM
GHSTLPR

Sequences:

>Translated_487_residues
MGKATGFLEFARQNEGYEPVVKRVKHYKEFVFALSDSEAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWKSAI
EVLHQTNNFPEFTGRICPAPCEAACTLGINELPVGIKSIEHAIIDKAWEEGWVQPQVPAHKTGKTVAVVGSGPAGMAAAQ
QLARAGHAVTVFEKNDRIGGLLRYGIPDFKMEKTLIDRRIEQMQAEGVTFRAGVMVTDGALPAGIKNYARETISAQALME
QFDAVVLAGGAEVPRDLPVPGRDLAGVHYALEFLIPQNKEVAGDGVNEIRAEGKHVIVIGGGDTGSDCVGTSNRHGATSV
TQFELLPQPPEEENKPLVWPYWPIKLRTSSSHDEGCSRDWSVATKELVGENGKVKALKACRVEWKDGKMQEVPGSEFVLQ
ADLVLLAMGFTNPVGSMLEAFGVDTDARKNAKASTEGDRAYYTNVPKVFAAGDVRRGQSLVVWAIREGRQAARSVDAFLM
GHSTLPR
>Mature_486_residues
GKATGFLEFARQNEGYEPVVKRVKHYKEFVFALSDSEAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWKSAIE
VLHQTNNFPEFTGRICPAPCEAACTLGINELPVGIKSIEHAIIDKAWEEGWVQPQVPAHKTGKTVAVVGSGPAGMAAAQQ
LARAGHAVTVFEKNDRIGGLLRYGIPDFKMEKTLIDRRIEQMQAEGVTFRAGVMVTDGALPAGIKNYARETISAQALMEQ
FDAVVLAGGAEVPRDLPVPGRDLAGVHYALEFLIPQNKEVAGDGVNEIRAEGKHVIVIGGGDTGSDCVGTSNRHGATSVT
QFELLPQPPEEENKPLVWPYWPIKLRTSSSHDEGCSRDWSVATKELVGENGKVKALKACRVEWKDGKMQEVPGSEFVLQA
DLVLLAMGFTNPVGSMLEAFGVDTDARKNAKASTEGDRAYYTNVPKVFAAGDVRRGQSLVVWAIREGRQAARSVDAFLMG
HSTLPR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=473, Percent_Identity=23.6786469344609, Blast_Score=94, Evalue=4e-19,
Organism=Escherichia coli, GI1789606, Length=486, Percent_Identity=35.59670781893, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1788811, Length=468, Percent_Identity=36.965811965812, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI87082180, Length=468, Percent_Identity=33.1196581196581, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI1788468, Length=460, Percent_Identity=25.8695652173913, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1789243, Length=437, Percent_Identity=28.3752860411899, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17570289, Length=506, Percent_Identity=44.2687747035573, Blast_Score=423, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI71984108, Length=471, Percent_Identity=23.1422505307856, Blast_Score=82, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6320030, Length=503, Percent_Identity=46.9184890656064, Blast_Score=460, Evalue=1e-130,
Organism=Drosophila melanogaster, GI28574881, Length=502, Percent_Identity=49.402390438247, Blast_Score=488, Evalue=1e-138,
Organism=Drosophila melanogaster, GI24665539, Length=502, Percent_Identity=49.402390438247, Blast_Score=488, Evalue=1e-138,
Organism=Drosophila melanogaster, GI24665547, Length=502, Percent_Identity=49.402390438247, Blast_Score=487, Evalue=1e-138,
Organism=Drosophila melanogaster, GI24665543, Length=502, Percent_Identity=49.402390438247, Blast_Score=487, Evalue=1e-138,
Organism=Drosophila melanogaster, GI24640763, Length=478, Percent_Identity=24.2677824267782, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI18858217, Length=478, Percent_Identity=24.2677824267782, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52948; Mature: 52817

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKATGFLEFARQNEGYEPVVKRVKHYKEFVFALSDSEAKIQGARCMDCGIPFCNNGCPV
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCC
NNIIPDFNDLVYRQDWKSAIEVLHQTNNFPEFTGRICPAPCEAACTLGINELPVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCHHHHCCCHHCCCCHHHHH
HAIIDKAWEEGWVQPQVPAHKTGKTVAVVGSGPAGMAAAQQLARAGHAVTVFEKNDRIGG
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCC
LLRYGIPDFKMEKTLIDRRIEQMQAEGVTFRAGVMVTDGALPAGIKNYARETISAQALME
EEECCCCCHHHHHHHHHHHHHHHHHCCEEEEECEEEECCCCCHHHHHHHHHHHHHHHHHH
QFDAVVLAGGAEVPRDLPVPGRDLAGVHYALEFLIPQNKEVAGDGVNEIRAEGKHVIVIG
HHCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEEEEE
GGDTGSDCVGTSNRHGATSVTQFELLPQPPEEENKPLVWPYWPIKLRTSSSHDEGCSRDW
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEECCCCEEEECCCCCCCCCCCCH
SVATKELVGENGKVKALKACRVEWKDGKMQEVPGSEFVLQADLVLLAMGFTNPVGSMLEA
HHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEECCEEHEEECCCCHHHHHHHH
FGVDTDARKNAKASTEGDRAYYTNVPKVFAAGDVRRGQSLVVWAIREGRQAARSVDAFLM
HCCCCCCCCCCCCCCCCCCEEECCCCCEEECCCCCCCCEEEEEEEHHHHHHHHHHHHHHH
GHSTLPR
CCCCCCC
>Mature Secondary Structure 
GKATGFLEFARQNEGYEPVVKRVKHYKEFVFALSDSEAKIQGARCMDCGIPFCNNGCPV
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCC
NNIIPDFNDLVYRQDWKSAIEVLHQTNNFPEFTGRICPAPCEAACTLGINELPVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCHHHHCCCHHCCCCHHHHH
HAIIDKAWEEGWVQPQVPAHKTGKTVAVVGSGPAGMAAAQQLARAGHAVTVFEKNDRIGG
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCC
LLRYGIPDFKMEKTLIDRRIEQMQAEGVTFRAGVMVTDGALPAGIKNYARETISAQALME
EEECCCCCHHHHHHHHHHHHHHHHHCCEEEEECEEEECCCCCHHHHHHHHHHHHHHHHHH
QFDAVVLAGGAEVPRDLPVPGRDLAGVHYALEFLIPQNKEVAGDGVNEIRAEGKHVIVIG
HHCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEEEEE
GGDTGSDCVGTSNRHGATSVTQFELLPQPPEEENKPLVWPYWPIKLRTSSSHDEGCSRDW
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEECCCCEEEECCCCCCCCCCCCH
SVATKELVGENGKVKALKACRVEWKDGKMQEVPGSEFVLQADLVLLAMGFTNPVGSMLEA
HHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEECCEEHEEECCCCHHHHHHHH
FGVDTDARKNAKASTEGDRAYYTNVPKVFAAGDVRRGQSLVVWAIREGRQAARSVDAFLM
HCCCCCCCCCCCCCCCCCCEEECCCCCEEECCCCCCCCEEEEEEEHHHHHHHHHHHHHHH
GHSTLPR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]