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The map label for this gene is mltA [H]

Identifier: 94312104

GI number: 94312104

Start: 3446652

End: 3447800

Strand: Reverse

Name: mltA [H]

Synonym: Rmet_3173

Alternate gene names: 94312104

Gene position: 3447800-3446652 (Counterclockwise)

Preceding gene: 94312106

Following gene: 94312103

Centisome position: 87.77

GC content: 67.36

Gene sequence:

>1149_bases
ATGGACAAGATGGCAAATCAGTATTCCCTGCGCGCCCCCCGCGCATCGTCGAGCAACACTTCCTGGCGCGGCTGGCTTGT
CCTGACGGGCGCTGCCGCACTGCTGGCCGGCTGCATGAGCGGGCCGCCCCCGCGTATCGAAGGTCCGGGCGGCGTGCCGG
GCGGGAAGGCGCCTGCTACCTCATCCGCGGCAGGCCGGTTGCAACCGGCGAGCTGGGCGGAAATCGGTGGCTGGAGCCAG
GACGACGTGCGTGCCGCCTGGCCCGCGCTGCTGGCGAGCTGCCAGGCGCTGAAAAAGCGCGCCGACTGGAACCGCGTCTG
CGCCGTCGGCCTGATGGTGGACGCCGGCGATCTCGAGGCGATGCGCGCGTACTTCGAAACCAACTTCCTGCCATACCGCG
TGGTCAACGTCGATGGGACGGACAACGGGTTGATCACCGGGTACTACGAACCGATCCTGCACGGCTCCCGCACGCGTCAG
GGTGCGTATCAGGTCCCGCTCTATCGCCGGCCGCCGCAACTCGGCAAGGGCATGTTGCCGCCGCGCGCCGAACTGCTGCA
GAACCCGGCGATGCGTGGCAGCGAACTGGTCTGGGTGGATGATCCGGTCGAAGCCGCGTTCCTGCAGATCCAGGGCTCCG
GCCGCATCCGCATGGCCGATGGCACGATGATGCGTGTCGGCTACGGCGGCACCAATGACCAGCCGTTCCGCTCATTCGGC
AAATGGCTGCTCGACCGTGGCGAGATCACGCCGGCGCAGGCCACGATGCAGGGCATCAAGGCATGGGCGCGTGCCAACCC
GGGACGCGTGGACGAAATGCTCAACGTCAATCCGCGCTTTGTGTTCTTCCGCGAATTGCCGCCCACCAACGAAGGACCGG
TAGGCGCGCTGGGCGTGCCGCTTACGGCGGAACGCTCGATAGCAGTTGATCCCGCAACCATCCCGCTTGGCGTGCCGGTT
TTCCTGTCGACCACACGGCCACTTTCCTCCGAACCGATTCAACGTCTGATGTTCGCGCAGGACACGGGCTCCGCCATCAA
GGGTGGCGTGCGCGCGGACTTCTTCTGGGGCGCAGGCGACGCCGCCGGCGAGACCGCCGGCCGCATGAAGCAGGGTGGCA
GGATGTGGGTCCTGATGCCGCGCAGCTGA

Upstream 100 bases:

>100_bases
AACTGGCTCATCGCGAGGGGGTCAGTCGACGGATCGGGTTGAGATTGTAGGACATGCGCGGGTTTGTGGACTGTCTGACC
GCCGGCCGTGTCCGCGAAGA

Downstream 100 bases:

>100_bases
CGTCATGTTATCGGCGGGCTACATACAGCAATGCCCGCCGTCCGAGTTAGTTCCCTGACACGGAGCAGGGTTATTCTCCT
GCCCCGTTCCCCGCGTTGAC

Product: Membrane-bound lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: Mlt38; Murein hydrolase A [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MDKMANQYSLRAPRASSSNTSWRGWLVLTGAAALLAGCMSGPPPRIEGPGGVPGGKAPATSSAAGRLQPASWAEIGGWSQ
DDVRAAWPALLASCQALKKRADWNRVCAVGLMVDAGDLEAMRAYFETNFLPYRVVNVDGTDNGLITGYYEPILHGSRTRQ
GAYQVPLYRRPPQLGKGMLPPRAELLQNPAMRGSELVWVDDPVEAAFLQIQGSGRIRMADGTMMRVGYGGTNDQPFRSFG
KWLLDRGEITPAQATMQGIKAWARANPGRVDEMLNVNPRFVFFRELPPTNEGPVGALGVPLTAERSIAVDPATIPLGVPV
FLSTTRPLSSEPIQRLMFAQDTGSAIKGGVRADFFWGAGDAAGETAGRMKQGGRMWVLMPRS

Sequences:

>Translated_382_residues
MDKMANQYSLRAPRASSSNTSWRGWLVLTGAAALLAGCMSGPPPRIEGPGGVPGGKAPATSSAAGRLQPASWAEIGGWSQ
DDVRAAWPALLASCQALKKRADWNRVCAVGLMVDAGDLEAMRAYFETNFLPYRVVNVDGTDNGLITGYYEPILHGSRTRQ
GAYQVPLYRRPPQLGKGMLPPRAELLQNPAMRGSELVWVDDPVEAAFLQIQGSGRIRMADGTMMRVGYGGTNDQPFRSFG
KWLLDRGEITPAQATMQGIKAWARANPGRVDEMLNVNPRFVFFRELPPTNEGPVGALGVPLTAERSIAVDPATIPLGVPV
FLSTTRPLSSEPIQRLMFAQDTGSAIKGGVRADFFWGAGDAAGETAGRMKQGGRMWVLMPRS
>Mature_382_residues
MDKMANQYSLRAPRASSSNTSWRGWLVLTGAAALLAGCMSGPPPRIEGPGGVPGGKAPATSSAAGRLQPASWAEIGGWSQ
DDVRAAWPALLASCQALKKRADWNRVCAVGLMVDAGDLEAMRAYFETNFLPYRVVNVDGTDNGLITGYYEPILHGSRTRQ
GAYQVPLYRRPPQLGKGMLPPRAELLQNPAMRGSELVWVDDPVEAAFLQIQGSGRIRMADGTMMRVGYGGTNDQPFRSFG
KWLLDRGEITPAQATMQGIKAWARANPGRVDEMLNVNPRFVFFRELPPTNEGPVGALGVPLTAERSIAVDPATIPLGVPV
FLSTTRPLSSEPIQRLMFAQDTGSAIKGGVRADFFWGAGDAAGETAGRMKQGGRMWVLMPRS

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division. Degrades murein glycan strands and insoluble, high-molecular weight murein sacculi [H]

COG id: COG2821

COG function: function code M; Membrane-bound lytic murein transglycosylase

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789179, Length=370, Percent_Identity=31.0810810810811, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010611
- InterPro:   IPR014733
- InterPro:   IPR005300 [H]

Pfam domain/function: PF06725 3D; PF03562 MltA [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 41144; Mature: 41144

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKMANQYSLRAPRASSSNTSWRGWLVLTGAAALLAGCMSGPPPRIEGPGGVPGGKAPAT
CCCCCCCCCCCCCCCCCCCCCEEEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
SSAAGRLQPASWAEIGGWSQDDVRAAWPALLASCQALKKRADWNRVCAVGLMVDAGDLEA
CCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHH
MRAYFETNFLPYRVVNVDGTDNGLITGYYEPILHGSRTRQGAYQVPLYRRPPQLGKGMLP
HHHHHHCCCCEEEEEEECCCCCCEEEEEHHHHHCCCCCCCCCEECCCCCCCHHHCCCCCC
PRAELLQNPAMRGSELVWVDDPVEAAFLQIQGSGRIRMADGTMMRVGYGGTNDQPFRSFG
CHHHHHCCCCCCCCEEEEECCCCCEEEEEEECCCEEEEECCEEEEEECCCCCCHHHHHHH
KWLLDRGEITPAQATMQGIKAWARANPGRVDEMLNVNPRFVFFRELPPTNEGPVGALGVP
HHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCEEEEEECCCCCCCCCCEECCC
LTAERSIAVDPATIPLGVPVFLSTTRPLSSEPIQRLMFAQDTGSAIKGGVRADFFWGAGD
CCCCCCEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHCCCCEEEEEECCCC
AAGETAGRMKQGGRMWVLMPRS
CCCHHHHHHHCCCCEEEEECCC
>Mature Secondary Structure
MDKMANQYSLRAPRASSSNTSWRGWLVLTGAAALLAGCMSGPPPRIEGPGGVPGGKAPAT
CCCCCCCCCCCCCCCCCCCCCEEEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
SSAAGRLQPASWAEIGGWSQDDVRAAWPALLASCQALKKRADWNRVCAVGLMVDAGDLEA
CCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHH
MRAYFETNFLPYRVVNVDGTDNGLITGYYEPILHGSRTRQGAYQVPLYRRPPQLGKGMLP
HHHHHHCCCCEEEEEEECCCCCCEEEEEHHHHHCCCCCCCCCEECCCCCCCHHHCCCCCC
PRAELLQNPAMRGSELVWVDDPVEAAFLQIQGSGRIRMADGTMMRVGYGGTNDQPFRSFG
CHHHHHCCCCCCCCEEEEECCCCCEEEEEEECCCEEEEECCEEEEEECCCCCCHHHHHHH
KWLLDRGEITPAQATMQGIKAWARANPGRVDEMLNVNPRFVFFRELPPTNEGPVGALGVP
HHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCEEEEEECCCCCCCCCCEECCC
LTAERSIAVDPATIPLGVPVFLSTTRPLSSEPIQRLMFAQDTGSAIKGGVRADFFWGAGD
CCCCCCEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHCCCCEEEEEECCCC
AAGETAGRMKQGGRMWVLMPRS
CCCHHHHHHHCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]