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The map label for this gene is yoaE [H]

Identifier: 94312082

GI number: 94312082

Start: 3414285

End: 3416390

Strand: Reverse

Name: yoaE [H]

Synonym: Rmet_3151

Alternate gene names: 94312082

Gene position: 3416390-3414285 (Counterclockwise)

Preceding gene: 94312083

Following gene: 94312081

Centisome position: 86.97

GC content: 66.76

Gene sequence:

>2106_bases
ATGGCCACCCGCATCGTCCGCGCCGCCTGCCCCCACGATTGCCCCGATACCTGTGCACTGCTCGTCACCGTCGAAGACGG
CCGGGCGATCAAGGTCGCCGGAGACCCCGAGCATCCGGGCACCCAGGGTGTGCTCTGCACCAAGGTTTCGCGCTATACCG
AACGCACCTATCACCCGGACCGGCTGTTGACCCCGCTGCGGCGAGTGGGCAAGAAGGGCGAGGGCAGGTTCGAGCCAATT
TCGTGGGACGAGGCGCTCGACGAAATTGCCGAGCGGCTGTCATCGATCGCCGCCCGGAATCCCGAGGCGATCGTTCCCTA
CAGCTACGCCGGCACGATGGGCCTGGTACAGGGCGAGAGCATGGCCGCGCGCTTCTTCCACAAGCTCGGCGCCTCGCTGC
TCGATCGCACGATCTGCGCCAGTGCGGGCGCCACGGCCCTGCGCCATACCTACGGCGCTAGCGTCGGCATGGACATGGAG
CAGGTGGTCGACGCGAAGCTGATCATTATCTGGGGCAGCAATCCGATTACGTCGAACCTGCATTTCTGGACGCGCGCCCA
GGAAGCCAAGCGCCGTGGCGCGACGCTGGTGGCGATCGACCCGTACCGCTCGCTGACGGCGGAGAAATGCCATCGTCATA
TCGCGCCGATGCCGGGCACCGACGCCGCGCTGGCGCTGGCGATGATGCACGTGCTGATTCGCGACGACCTGATCGACCAC
GACTACGTCGCCCGCAATACCGTTGGCTTCGACGCGCTGCGCGAACGGGCGATGCAGTTTTCGCCGGAAACGGCGGCGCG
GATCTGCGGGATTCCGGTCGATGACATCGAGTGGCTCGCCGGGCTGTACGGCAAACTCGCCGTGCGCGAGCGCCAGCCGG
TGGCGATCCGCCTGAATTACGGGATGCAGCGCGTGCACGGTGGCGGCCAGGCAGTGCGTGCCGTGGCGTGCCTGCCTTCG
CTGGTCGGTGCATGGCGCGAGGCCGCAGGCGGCCTGCTGTTGTCGACGTCGGGCTTCTTCCCGGTCGACAACGCGGCGCT
GCAACGACCCGACCTGCTGCCTGGCTGGCCGAACTTGCCGCGCACGATCAACATGAGCACGATCGGCGACGCGCTGCTGG
ACGACGGCACCACCGGCAACGGCCCGAAGATCGAAGCCGTGGTGGTCTACAACAGCAACCCCGTGGCAGTGGCGCCGGAA
TCGGGGAAGGTGGCAGCCGGGTTTGGACGCGACGACCTGTTCACGGTTGTACTGGAACACTTCCGGACCGATACGGCCGA
TTACGCCGATATCGTGCTGCCGGCCACGACGCAGCTCGAGCACATTGATGTGCACAAGGCCTACGGCCACACTTACTTCC
TGGCGAACAACCAGGCGGTGGAGCCGCTCGGGCAGGCGCTGCCGAACACCGAGATCTTCCGCCGGCTGGCCGCGCGCATG
GGATTCACGGAGTCCTGCTTTGCCGACAGCGACGAGGCGATCGCATCGCAGGCCGTCCTGCGTGAAGACAAGCGCGCGGC
GGGTATTAGCTGGGATTCCCTCAAGGCCCGGGGCTGGCAGAAGCTGAATATCGCCAAGGCGCCGTTCGCCGAGGGCGGCT
TCCCGTCGGCATCGGGCAAGTGCGAGTTCTATTCGGAGACAATGGCGCGAGAGGGGCTTGATCCCGTTCCCGCCTACGTG
CCGCCTTACGAAGCGCCTTCCTCGGCCCCCGAACTGTTCGCGCGTTACCCGCTGGCGATGATCTCGCCGCCGGCACGTAA
CTTCCTCAACAGCACGTTCGTCAATGTCGACAGCCTGCGCGCCACCGAAGGCGAGCCGCATCTCGACATCCATCCGCTCG
ACGCCGCGGCGCGTGGCATTGCCAATGGTGTGCAGGTCCGCGCCTTCAACGATCGCGGCAGCATGCTGGCGCGCGCCCGC
GTGACCGACCGCGCCCGGCAGGGCCTGGTCGTCGGCCTGTCGATCTGGTGGAAGAAGCTCGCGCCGGATGGCAAGAACGC
CAACGAACTGACCAGCCAGCGATTGACGGACCTGGGGCGCGCGCCGGTGTTCTACGATTGCCTGGTCGAAGTGGAGCCGG
TGCCCGCGGAGGCGCTGGCATCATGA

Upstream 100 bases:

>100_bases
GAAGAGGCGAAGTTCGATTCGCCCTGACGCTGGCCCGTTCGCCGTACTTCATTGCCCACACCGGGCGCCCCACTGAAGTT
ATCTGCCTGCCGATACCGCC

Downstream 100 bases:

>100_bases
TCCGCTGGCCGGCCCGCCGGCGCCTGGCGAGTGCCCTGGCGGTGCTCGGCGCGGCGTTGCTGGTGGCCGGCTGCGACACA
CTCGGCTATTACTATCAGTC

Product: molybdopterin oxidoreductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 701; Mature: 700

Protein sequence:

>701_residues
MATRIVRAACPHDCPDTCALLVTVEDGRAIKVAGDPEHPGTQGVLCTKVSRYTERTYHPDRLLTPLRRVGKKGEGRFEPI
SWDEALDEIAERLSSIAARNPEAIVPYSYAGTMGLVQGESMAARFFHKLGASLLDRTICASAGATALRHTYGASVGMDME
QVVDAKLIIIWGSNPITSNLHFWTRAQEAKRRGATLVAIDPYRSLTAEKCHRHIAPMPGTDAALALAMMHVLIRDDLIDH
DYVARNTVGFDALRERAMQFSPETAARICGIPVDDIEWLAGLYGKLAVRERQPVAIRLNYGMQRVHGGGQAVRAVACLPS
LVGAWREAAGGLLLSTSGFFPVDNAALQRPDLLPGWPNLPRTINMSTIGDALLDDGTTGNGPKIEAVVVYNSNPVAVAPE
SGKVAAGFGRDDLFTVVLEHFRTDTADYADIVLPATTQLEHIDVHKAYGHTYFLANNQAVEPLGQALPNTEIFRRLAARM
GFTESCFADSDEAIASQAVLREDKRAAGISWDSLKARGWQKLNIAKAPFAEGGFPSASGKCEFYSETMAREGLDPVPAYV
PPYEAPSSAPELFARYPLAMISPPARNFLNSTFVNVDSLRATEGEPHLDIHPLDAAARGIANGVQVRAFNDRGSMLARAR
VTDRARQGLVVGLSIWWKKLAPDGKNANELTSQRLTDLGRAPVFYDCLVEVEPVPAEALAS

Sequences:

>Translated_701_residues
MATRIVRAACPHDCPDTCALLVTVEDGRAIKVAGDPEHPGTQGVLCTKVSRYTERTYHPDRLLTPLRRVGKKGEGRFEPI
SWDEALDEIAERLSSIAARNPEAIVPYSYAGTMGLVQGESMAARFFHKLGASLLDRTICASAGATALRHTYGASVGMDME
QVVDAKLIIIWGSNPITSNLHFWTRAQEAKRRGATLVAIDPYRSLTAEKCHRHIAPMPGTDAALALAMMHVLIRDDLIDH
DYVARNTVGFDALRERAMQFSPETAARICGIPVDDIEWLAGLYGKLAVRERQPVAIRLNYGMQRVHGGGQAVRAVACLPS
LVGAWREAAGGLLLSTSGFFPVDNAALQRPDLLPGWPNLPRTINMSTIGDALLDDGTTGNGPKIEAVVVYNSNPVAVAPE
SGKVAAGFGRDDLFTVVLEHFRTDTADYADIVLPATTQLEHIDVHKAYGHTYFLANNQAVEPLGQALPNTEIFRRLAARM
GFTESCFADSDEAIASQAVLREDKRAAGISWDSLKARGWQKLNIAKAPFAEGGFPSASGKCEFYSETMAREGLDPVPAYV
PPYEAPSSAPELFARYPLAMISPPARNFLNSTFVNVDSLRATEGEPHLDIHPLDAAARGIANGVQVRAFNDRGSMLARAR
VTDRARQGLVVGLSIWWKKLAPDGKNANELTSQRLTDLGRAPVFYDCLVEVEPVPAEALAS
>Mature_700_residues
ATRIVRAACPHDCPDTCALLVTVEDGRAIKVAGDPEHPGTQGVLCTKVSRYTERTYHPDRLLTPLRRVGKKGEGRFEPIS
WDEALDEIAERLSSIAARNPEAIVPYSYAGTMGLVQGESMAARFFHKLGASLLDRTICASAGATALRHTYGASVGMDMEQ
VVDAKLIIIWGSNPITSNLHFWTRAQEAKRRGATLVAIDPYRSLTAEKCHRHIAPMPGTDAALALAMMHVLIRDDLIDHD
YVARNTVGFDALRERAMQFSPETAARICGIPVDDIEWLAGLYGKLAVRERQPVAIRLNYGMQRVHGGGQAVRAVACLPSL
VGAWREAAGGLLLSTSGFFPVDNAALQRPDLLPGWPNLPRTINMSTIGDALLDDGTTGNGPKIEAVVVYNSNPVAVAPES
GKVAAGFGRDDLFTVVLEHFRTDTADYADIVLPATTQLEHIDVHKAYGHTYFLANNQAVEPLGQALPNTEIFRRLAARMG
FTESCFADSDEAIASQAVLREDKRAAGISWDSLKARGWQKLNIAKAPFAEGGFPSASGKCEFYSETMAREGLDPVPAYVP
PYEAPSSAPELFARYPLAMISPPARNFLNSTFVNVDSLRATEGEPHLDIHPLDAAARGIANGVQVRAFNDRGSMLARARV
TDRARQGLVVGLSIWWKKLAPDGKNANELTSQRLTDLGRAPVFYDCLVEVEPVPAEALAS

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=783, Percent_Identity=29.1187739463602, Blast_Score=222, Evalue=6e-59,
Organism=Escherichia coli, GI1787870, Length=780, Percent_Identity=28.7179487179487, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI171474008, Length=781, Percent_Identity=27.6568501920615, Blast_Score=199, Evalue=5e-52,
Organism=Escherichia coli, GI3868721, Length=731, Percent_Identity=25.3077975376197, Blast_Score=169, Evalue=6e-43,
Organism=Escherichia coli, GI1787231, Length=736, Percent_Identity=25.9510869565217, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87081994, Length=711, Percent_Identity=25.7383966244726, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI145693196, Length=665, Percent_Identity=24.9624060150376, Blast_Score=132, Evalue=9e-32,
Organism=Escherichia coli, GI1788534, Length=819, Percent_Identity=22.2222222222222, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI3868720, Length=471, Percent_Identity=22.9299363057325, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI3868719, Length=621, Percent_Identity=21.0950080515298, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1787477, Length=296, Percent_Identity=25.6756756756757, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1787778, Length=239, Percent_Identity=26.7782426778243, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 76052; Mature: 75921

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRIVRAACPHDCPDTCALLVTVEDGRAIKVAGDPEHPGTQGVLCTKVSRYTERTYHPD
CCCCHHHHCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCH
RLLTPLRRVGKKGEGRFEPISWDEALDEIAERLSSIAARNPEAIVPYSYAGTMGLVQGES
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCHH
MAARFFHKLGASLLDRTICASAGATALRHTYGASVGMDMEQVVDAKLIIIWGSNPITSNL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHCCEEEEEEECCCCCCCCC
HFWTRAQEAKRRGATLVAIDPYRSLTAEKCHRHIAPMPGTDAALALAMMHVLIRDDLIDH
CHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC
DYVARNTVGFDALRERAMQFSPETAARICGIPVDDIEWLAGLYGKLAVRERQPVAIRLNY
CHHHHCCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECC
GMQRVHGGGQAVRAVACLPSLVGAWREAAGGLLLSTSGFFPVDNAALQRPDLLPGWPNLP
CHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHCCCCCCCCCCCCCC
RTINMSTIGDALLDDGTTGNGPKIEAVVVYNSNPVAVAPESGKVAAGFGRDDLFTVVLEH
CEEEHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEECCCCCCEEECCCCHHHHHHHHHH
FRTDTADYADIVLPATTQLEHIDVHKAYGHTYFLANNQAVEPLGQALPNTEIFRRLAARM
HCCCCCCCCEEEECCCCCCHHEEHHHHCCCEEEEECCCCHHHHHHHCCCHHHHHHHHHHC
GFTESCFADSDEAIASQAVLREDKRAAGISWDSLKARGWQKLNIAKAPFAEGGFPSASGK
CCCHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEECCCCCCCCCCCCCCCC
CEFYSETMAREGLDPVPAYVPPYEAPSSAPELFARYPLAMISPPARNFLNSTFVNVDSLR
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHCCCHHHHHHHHHHCCHHHCC
ATEGEPHLDIHPLDAAARGIANGVQVRAFNDRGSMLARARVTDRARQGLVVGLSIWWKKL
CCCCCCCCEECCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCEEEEHHHHHHHH
APDGKNANELTSQRLTDLGRAPVFYDCLVEVEPVPAEALAS
CCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHCC
>Mature Secondary Structure 
ATRIVRAACPHDCPDTCALLVTVEDGRAIKVAGDPEHPGTQGVLCTKVSRYTERTYHPD
CCCHHHHCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCH
RLLTPLRRVGKKGEGRFEPISWDEALDEIAERLSSIAARNPEAIVPYSYAGTMGLVQGES
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCHH
MAARFFHKLGASLLDRTICASAGATALRHTYGASVGMDMEQVVDAKLIIIWGSNPITSNL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHCCEEEEEEECCCCCCCCC
HFWTRAQEAKRRGATLVAIDPYRSLTAEKCHRHIAPMPGTDAALALAMMHVLIRDDLIDH
CHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC
DYVARNTVGFDALRERAMQFSPETAARICGIPVDDIEWLAGLYGKLAVRERQPVAIRLNY
CHHHHCCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECC
GMQRVHGGGQAVRAVACLPSLVGAWREAAGGLLLSTSGFFPVDNAALQRPDLLPGWPNLP
CHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHCCCCCCCCCCCCCC
RTINMSTIGDALLDDGTTGNGPKIEAVVVYNSNPVAVAPESGKVAAGFGRDDLFTVVLEH
CEEEHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEECCCCCCEEECCCCHHHHHHHHHH
FRTDTADYADIVLPATTQLEHIDVHKAYGHTYFLANNQAVEPLGQALPNTEIFRRLAARM
HCCCCCCCCEEEECCCCCCHHEEHHHHCCCEEEEECCCCHHHHHHHCCCHHHHHHHHHHC
GFTESCFADSDEAIASQAVLREDKRAAGISWDSLKARGWQKLNIAKAPFAEGGFPSASGK
CCCHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEECCCCCCCCCCCCCCCC
CEFYSETMAREGLDPVPAYVPPYEAPSSAPELFARYPLAMISPPARNFLNSTFVNVDSLR
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHCCCHHHHHHHHHHCCHHHCC
ATEGEPHLDIHPLDAAARGIANGVQVRAFNDRGSMLARARVTDRARQGLVVGLSIWWKKL
CCCCCCCCEECCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCEEEEHHHHHHHH
APDGKNANELTSQRLTDLGRAPVFYDCLVEVEPVPAEALAS
CCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]