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The map label for this gene is mraW

Identifier: 94312067

GI number: 94312067

Start: 3400730

End: 3401707

Strand: Reverse

Name: mraW

Synonym: Rmet_3136

Alternate gene names: 94312067

Gene position: 3401707-3400730 (Counterclockwise)

Preceding gene: 94312068

Following gene: 94312066

Centisome position: 86.6

GC content: 65.44

Gene sequence:

>978_bases
ATGAGCCCTACCGAATCGCCGGGGACCACCACCCTGCGCCATCGCACCGTGTTGCTGGACGAGGCCGTCGATGCGCTCGT
CTGGCGGCCCGACGGCGCCTATGTGGACGGCACCTTCGGCAGAGGGGGGCACAGCCGGGCAGTACTGGCCCGGCTGGGGC
CGGCCGGCACCCTGGTCGCCTTCGACAAGGATCCGGCAGCAATCGCAGAAGCGGGCACCATCAAGGATGCCCGCTTCTCC
ATTGAGCACGCGAGCTTTGCGGAAATGGGCGATCGGCTGGCCGGCCGTGGCCCGGTGGCTGGCGTGCTGCTCGACCTGGG
AATCAGCTCGCCCCAGATCGACGAGGCAGCGAGGGGGTTTTCCTTTCGTTTCGAGGGCCCGCTGGACATGCGCATGGACA
CCACGCGCGGCATTACCGCCGCCGAGTGGCTTGCACAAGCGGACGAGCAGGACATTGCCAGGGTGATACGAGACTATGGG
GAAGAACGGTTTGCTCTACAGATTGCAAAGGCGATTGTTGCTCGCCGGCGCGAATCCGGCGATGGCGGAGCACTCGCCAC
TACTTCAGACCTTGCCGCGCTCGTGGCGAAAGCCGTCAAAACACGCGAGAAGGGCCAGGACCCTGCGACCCGCACCTTTC
AAGCTTTACGGATTTACATCAATCAAGAGCTTGAGGACCTCGAAAGAGGTCTGAAGGCGGCGTACGAACTGCTTCAGGTC
GGGGGTCGCCTCGTGGTGATCAGCTTTCACTCGCTCGAGGACCGTATCGTCAAACGGTTCATGCAGGCGCACGCCCGCCC
CGAGCGGGATGCCGATCCGGCCCTGCGCCGCGCACCGTTGCGCGCGGCTGACCTGCCGCAGCCGACGATGAAACTGCTGG
GCCGATTCAAGCCGGGCGCCGAAGAGGTGGCCGGCAACCCGCGCGCCCGTTCCGCGGTGATGCGCGTGGCCGAAAAACTC
GGAGAGCAGTCGGCATGA

Upstream 100 bases:

>100_bases
TTCGAAGTTTGGGATGCCGCGACCTACGCCGCCAAGGAACAGGCAGCGATGGCGCAAGGCATGCCGGAAGCATTGAAGAA
TTTCTCCTTCTGAAGAGGTC

Downstream 100 bases:

>100_bases
ACCGCCTGACCTTCTTCCTCCTTGCCGCCCTGTTGTTCTGCGCGCTGTCGCTGGTCAGCGCGCAGCATCAGGCACGCACG
CTGTTCGTGGCACTCGAACG

Product: 16S rRNA m(4)C1402 methyltranserfase

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSPTESPGTTTLRHRTVLLDEAVDALVWRPDGAYVDGTFGRGGHSRAVLARLGPAGTLVAFDKDPAAIAEAGTIKDARFS
IEHASFAEMGDRLAGRGPVAGVLLDLGISSPQIDEAARGFSFRFEGPLDMRMDTTRGITAAEWLAQADEQDIARVIRDYG
EERFALQIAKAIVARRRESGDGGALATTSDLAALVAKAVKTREKGQDPATRTFQALRIYINQELEDLERGLKAAYELLQV
GGRLVVISFHSLEDRIVKRFMQAHARPERDADPALRRAPLRAADLPQPTMKLLGRFKPGAEEVAGNPRARSAVMRVAEKL
GEQSA

Sequences:

>Translated_325_residues
MSPTESPGTTTLRHRTVLLDEAVDALVWRPDGAYVDGTFGRGGHSRAVLARLGPAGTLVAFDKDPAAIAEAGTIKDARFS
IEHASFAEMGDRLAGRGPVAGVLLDLGISSPQIDEAARGFSFRFEGPLDMRMDTTRGITAAEWLAQADEQDIARVIRDYG
EERFALQIAKAIVARRRESGDGGALATTSDLAALVAKAVKTREKGQDPATRTFQALRIYINQELEDLERGLKAAYELLQV
GGRLVVISFHSLEDRIVKRFMQAHARPERDADPALRRAPLRAADLPQPTMKLLGRFKPGAEEVAGNPRARSAVMRVAEKL
GEQSA
>Mature_324_residues
SPTESPGTTTLRHRTVLLDEAVDALVWRPDGAYVDGTFGRGGHSRAVLARLGPAGTLVAFDKDPAAIAEAGTIKDARFSI
EHASFAEMGDRLAGRGPVAGVLLDLGISSPQIDEAARGFSFRFEGPLDMRMDTTRGITAAEWLAQADEQDIARVIRDYGE
ERFALQIAKAIVARRRESGDGGALATTSDLAALVAKAVKTREKGQDPATRTFQALRIYINQELEDLERGLKAAYELLQVG
GRLVVISFHSLEDRIVKRFMQAHARPERDADPALRRAPLRAADLPQPTMKLLGRFKPGAEEVAGNPRARSAVMRVAEKLG
EQSA

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=347, Percent_Identity=39.193083573487, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI165377202, Length=198, Percent_Identity=39.8989898989899, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI1786270, Length=313, Percent_Identity=55.2715654952077, Blast_Score=327, Evalue=5e-91,
Organism=Drosophila melanogaster, GI62472493, Length=343, Percent_Identity=38.7755102040816, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI28571637, Length=343, Percent_Identity=38.7755102040816, Blast_Score=187, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_RALME (Q1LIL8)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_585277.1
- ProteinModelPortal:   Q1LIL8
- SMR:   Q1LIL8
- STRING:   Q1LIL8
- GeneID:   4039965
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_3136
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- PhylomeDB:   Q1LIL8
- ProtClustDB:   PRK00050
- BioCyc:   RMET266264:RMET_3136-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35220; Mature: 35089

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: BINDING 62-62 BINDING 86-86 BINDING 105-105 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTESPGTTTLRHRTVLLDEAVDALVWRPDGAYVDGTFGRGGHSRAVLARLGPAGTLVA
CCCCCCCCCCHHHHHHHHHHHHHHHEEECCCCCEECCCCCCCCCCHHHHHHCCCCCEEEE
FDKDPAAIAEAGTIKDARFSIEHASFAEMGDRLAGRGPVAGVLLDLGISSPQIDEAARGF
ECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHCCC
SFRFEGPLDMRMDTTRGITAAEWLAQADEQDIARVIRDYGEERFALQIAKAIVARRRESG
CEEECCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
DGGALATTSDLAALVAKAVKTREKGQDPATRTFQALRIYINQELEDLERGLKAAYELLQV
CCCCEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGRLVVISFHSLEDRIVKRFMQAHARPERDADPALRRAPLRAADLPQPTMKLLGRFKPGA
CCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHCCCCCH
EEVAGNPRARSAVMRVAEKLGEQSA
HHHCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SPTESPGTTTLRHRTVLLDEAVDALVWRPDGAYVDGTFGRGGHSRAVLARLGPAGTLVA
CCCCCCCCCHHHHHHHHHHHHHHHEEECCCCCEECCCCCCCCCCHHHHHHCCCCCEEEE
FDKDPAAIAEAGTIKDARFSIEHASFAEMGDRLAGRGPVAGVLLDLGISSPQIDEAARGF
ECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHCCC
SFRFEGPLDMRMDTTRGITAAEWLAQADEQDIARVIRDYGEERFALQIAKAIVARRRESG
CEEECCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
DGGALATTSDLAALVAKAVKTREKGQDPATRTFQALRIYINQELEDLERGLKAAYELLQV
CCCCEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGRLVVISFHSLEDRIVKRFMQAHARPERDADPALRRAPLRAADLPQPTMKLLGRFKPGA
CCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHCCCCCH
EEVAGNPRARSAVMRVAEKLGEQSA
HHHCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA