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The map label for this gene is mutT [H]

Identifier: 94312046

GI number: 94312046

Start: 3375205

End: 3375648

Strand: Reverse

Name: mutT [H]

Synonym: Rmet_3115

Alternate gene names: 94312046

Gene position: 3375648-3375205 (Counterclockwise)

Preceding gene: 94312047

Following gene: 94312045

Centisome position: 85.94

GC content: 65.54

Gene sequence:

>444_bases
ATGAGCGGTCAGGCGGACACGGTGAAGACCGCCGACGGGCAGACGCGCAAGGTCACGGAAGTGGCGGTTGGCGTGATGAT
CCAGGCGGACGGCAAGTTTCTGCTGGCGCAGCGTCCGGCGGGCAAGCCGTACGAGGGCTACTGGGAATTTCCGGGCGGCA
AGCTCGAGCCCGGCGAATCGGTTGAGGCGGCACTGGCGCGCGAACTGCACGAAGAACTCGGCCTCGACATCGTGAGAAGC
GAACACTGGCACACGCTGGAGCATGATTACCCTCATGCCTATGTGCGCCTGTATTTCCGCAAGATCACGGAATGGCGTGG
CGATCCGGTCGGGCGGGAGGGCCAGGCATTCTCCTGGCAGACAGCGCCCGTGTCGGTGGGGCCGCTCCTGCCGGCGACGA
TCCCAGTCGTCGACTGGCTGGCGGAAGAATCCCGCGCAAGCTGA

Upstream 100 bases:

>100_bases
CGCGCTCCGGGCGCGTGGCGTGGCAGTTCGCGCGCGACTGGGGCGGCAAGCATGGCAAGCCGTTCGTGGCCGACCCCGCG
ACTGATTCAGGCAGCAAGGC

Downstream 100 bases:

>100_bases
AATCGATGCCCGGCTCTTGCCGGGCAATTTTTTTCGACCACTCACAGGAGGCTCAATGCAACGTGTCGATCGTATCGATG
ACGCTGTTTCTCTGCCCGAA

Product: mutator MutT protein (7,8-dihydro-8-oxoguanine-triphosphatase)

Products: CMP; diphosphate [C]

Alternate protein names: 7,8-dihydro-8-oxoguanine-triphosphatase; 8-oxo-dGTPase; dGTP pyrophosphohydrolase [H]

Number of amino acids: Translated: 147; Mature: 146

Protein sequence:

>147_residues
MSGQADTVKTADGQTRKVTEVAVGVMIQADGKFLLAQRPAGKPYEGYWEFPGGKLEPGESVEAALARELHEELGLDIVRS
EHWHTLEHDYPHAYVRLYFRKITEWRGDPVGREGQAFSWQTAPVSVGPLLPATIPVVDWLAEESRAS

Sequences:

>Translated_147_residues
MSGQADTVKTADGQTRKVTEVAVGVMIQADGKFLLAQRPAGKPYEGYWEFPGGKLEPGESVEAALARELHEELGLDIVRS
EHWHTLEHDYPHAYVRLYFRKITEWRGDPVGREGQAFSWQTAPVSVGPLLPATIPVVDWLAEESRAS
>Mature_146_residues
SGQADTVKTADGQTRKVTEVAVGVMIQADGKFLLAQRPAGKPYEGYWEFPGGKLEPGESVEAALARELHEELGLDIVRSE
HWHTLEHDYPHAYVRLYFRKITEWRGDPVGREGQAFSWQTAPVSVGPLLPATIPVVDWLAEESRAS

Specific function: Involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal efficiency thus lea

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788056, Length=123, Percent_Identity=39.0243902439024, Blast_Score=79, Evalue=1e-16,
Organism=Escherichia coli, GI1786288, Length=122, Percent_Identity=32.7868852459016, Blast_Score=77, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003561
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 16341; Mature: 16210

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGQADTVKTADGQTRKVTEVAVGVMIQADGKFLLAQRPAGKPYEGYWEFPGGKLEPGES
CCCCCCCEECCCCCCHHHHHHHEEEEEECCCCEEEEECCCCCCCCCEEECCCCCCCCCCH
VEAALARELHEELGLDIVRSEHWHTLEHDYPHAYVRLYFRKITEWRGDPVGREGQAFSWQ
HHHHHHHHHHHHHCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
TAPVSVGPLLPATIPVVDWLAEESRAS
CCCEECCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SGQADTVKTADGQTRKVTEVAVGVMIQADGKFLLAQRPAGKPYEGYWEFPGGKLEPGES
CCCCCCEECCCCCCHHHHHHHEEEEEECCCCEEEEECCCCCCCCCEEECCCCCCCCCCH
VEAALARELHEELGLDIVRSEHWHTLEHDYPHAYVRLYFRKITEWRGDPVGREGQAFSWQ
HHHHHHHHHHHHHCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
TAPVSVGPLLPATIPVVDWLAEESRAS
CCCEECCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe; Mn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CTP; H2O [C]

Specific reaction: CTP + H2O = CMP + diphosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]