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The map label for this gene is nrdB [H]

Identifier: 94312018

GI number: 94312018

Start: 3342183

End: 3343370

Strand: Reverse

Name: nrdB [H]

Synonym: Rmet_3087

Alternate gene names: 94312018

Gene position: 3343370-3342183 (Counterclockwise)

Preceding gene: 94312019

Following gene: 94312016

Centisome position: 85.11

GC content: 62.37

Gene sequence:

>1188_bases
ATGCTGAGCTGGGACGACGACGTTCAAGCCACACCGCAGGCCGCGCCGCAGCCTGCCCTGCAACCCGCTGCCGTCGCCAC
GTCGGTCGACCAGCAAGGCGTGCTGCCGCCGGCAGCCACCGAGCCGGGCATCCTCGGCAACAACCCCAACGCCGCCGCCG
CGCAAAGCACGCGCCGCGTGAACGCCGCCGACAAGCGCGTGATCAACGGCGCCACGGACGTGAACCAGCTCGTGCCGTTC
AAGTACAAGTGGGCATGGGAAAAGTATCTGGCCGGTTGCGCGAACCACTGGATGCCGCAGGAAATCAACATGTCGCGCGA
CATCGCCCTGTGGAAGGACCCGAACGGCCTGACCGAGGACGAGCGCCGCATCATCAAGCGCAACCTGGGCTTCTTCGTGA
CGGCCGATTCGCTGGCCGCCAACAATATCGTGCTGGGCACGTACCGCCAGATCACGGCGCCGGAATGCCGCCAGTACCTG
TTACGCCAGGCCTTCGAAGAGGCCATCCACACGCACGCGTACCAGTACATCGTGGAATCGCTCGGTCTGAACGAAGCCGA
GATCTTCAACGCGTACCATGAAGTGCAGTCGATCCGCGACAAGGACGAGTTCCTGATCCCGTTCATCGACACGCTGACCG
ACCCGTCGTTCAAGACCGGCACGCCGGAGAACGACCAGAAGCTGCTGAAGTCGCTGATCGTGTTCGCCTGCATTATGGAA
GGCCTGTTCTTCTACGTGGGCTTCACGCAGATCCTGGCGATGGGCCGCCAGAACAAGATGACCGGCGCTGCCGAGCAGTA
CCAGTACATCCTGCGCGACGAATCCCTGCACTGCAATTTCGGCATCGACCTGATCAACCAGATCAAGCTCGAGAACCCGC
ACCTGTGGACGGCCGAGTTCAAGGCCGAGATCACCGAGCTGTTCAAGAAGGCCGTGGACCTGGAATACCGCTACGCCGAG
GACACCATGCCGCGCGGCGTGCTGGGCCTGAACGCACCGATGTTCAAGGGCTACCTGCGCTTCATCTGCAACCGCCGTTG
CCAGCAGATCGGCCTGGACCAGCTCTTCCCGAACGAGGAAAACCCGTTCCCGTGGATGAGCGAAATGATCGACCTGAAGA
AAGAGCGTAACTTCTTCGAGACGCGCGTCATCGAGTACCAGACGGGCGGCGCGCTGTCGTGGGATTGA

Upstream 100 bases:

>100_bases
CTGTGCGGGGGAGGGGCCGGGGGAGAGGGCCAGGCACATCGATAGCCGATGCGCCTAAGCTGACCACAGCCGCCCCTGAT
CGAATCATCGGAGAAACAAC

Downstream 100 bases:

>100_bases
GGGGTCGGCTGCCGCGTTGAGAAGACGGCAGCCAGAATGGGTAGTGTGCGGACATCGGGCGGAGCCTGATGTCCGTTTTT
GTTTCTTGGCGGCTTATTCC

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MLSWDDDVQATPQAAPQPALQPAAVATSVDQQGVLPPAATEPGILGNNPNAAAAQSTRRVNAADKRVINGATDVNQLVPF
KYKWAWEKYLAGCANHWMPQEINMSRDIALWKDPNGLTEDERRIIKRNLGFFVTADSLAANNIVLGTYRQITAPECRQYL
LRQAFEEAIHTHAYQYIVESLGLNEAEIFNAYHEVQSIRDKDEFLIPFIDTLTDPSFKTGTPENDQKLLKSLIVFACIME
GLFFYVGFTQILAMGRQNKMTGAAEQYQYILRDESLHCNFGIDLINQIKLENPHLWTAEFKAEITELFKKAVDLEYRYAE
DTMPRGVLGLNAPMFKGYLRFICNRRCQQIGLDQLFPNEENPFPWMSEMIDLKKERNFFETRVIEYQTGGALSWD

Sequences:

>Translated_395_residues
MLSWDDDVQATPQAAPQPALQPAAVATSVDQQGVLPPAATEPGILGNNPNAAAAQSTRRVNAADKRVINGATDVNQLVPF
KYKWAWEKYLAGCANHWMPQEINMSRDIALWKDPNGLTEDERRIIKRNLGFFVTADSLAANNIVLGTYRQITAPECRQYL
LRQAFEEAIHTHAYQYIVESLGLNEAEIFNAYHEVQSIRDKDEFLIPFIDTLTDPSFKTGTPENDQKLLKSLIVFACIME
GLFFYVGFTQILAMGRQNKMTGAAEQYQYILRDESLHCNFGIDLINQIKLENPHLWTAEFKAEITELFKKAVDLEYRYAE
DTMPRGVLGLNAPMFKGYLRFICNRRCQQIGLDQLFPNEENPFPWMSEMIDLKKERNFFETRVIEYQTGGALSWD
>Mature_395_residues
MLSWDDDVQATPQAAPQPALQPAAVATSVDQQGVLPPAATEPGILGNNPNAAAAQSTRRVNAADKRVINGATDVNQLVPF
KYKWAWEKYLAGCANHWMPQEINMSRDIALWKDPNGLTEDERRIIKRNLGFFVTADSLAANNIVLGTYRQITAPECRQYL
LRQAFEEAIHTHAYQYIVESLGLNEAEIFNAYHEVQSIRDKDEFLIPFIDTLTDPSFKTGTPENDQKLLKSLIVFACIME
GLFFYVGFTQILAMGRQNKMTGAAEQYQYILRDESLHCNFGIDLINQIKLENPHLWTAEFKAEITELFKKAVDLEYRYAE
DTMPRGVLGLNAPMFKGYLRFICNRRCQQIGLDQLFPNEENPFPWMSEMIDLKKERNFFETRVIEYQTGGALSWD

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI289177074, Length=370, Percent_Identity=24.8648648648649, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI42544136, Length=360, Percent_Identity=24.4444444444444, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI260064013, Length=320, Percent_Identity=26.875, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI4557845, Length=404, Percent_Identity=25.990099009901, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI289177076, Length=300, Percent_Identity=28, Blast_Score=119, Evalue=5e-27,
Organism=Escherichia coli, GI1788567, Length=292, Percent_Identity=26.3698630136986, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17551968, Length=354, Percent_Identity=26.5536723163842, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI193206379, Length=399, Percent_Identity=21.3032581453634, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17567153, Length=224, Percent_Identity=25.4464285714286, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322434, Length=330, Percent_Identity=30.3030303030303, Blast_Score=149, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6321619, Length=320, Percent_Identity=22.5, Blast_Score=87, Evalue=6e-18,
Organism=Drosophila melanogaster, GI18079279, Length=320, Percent_Identity=26.875, Blast_Score=139, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 45037; Mature: 45037

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSWDDDVQATPQAAPQPALQPAAVATSVDQQGVLPPAATEPGILGNNPNAAAAQSTRRV
CCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHC
NAADKRVINGATDVNQLVPFKYKWAWEKYLAGCANHWMPQEINMSRDIALWKDPNGLTED
CHHHHHHHCCCCCHHHHCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHH
ERRIIKRNLGFFVTADSLAANNIVLGTYRQITAPECRQYLLRQAFEEAIHTHAYQYIVES
HHHHHHHCCCEEEEECCCCCCCEEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGLNEAEIFNAYHEVQSIRDKDEFLIPFIDTLTDPSFKTGTPENDQKLLKSLIVFACIME
HCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
GLFFYVGFTQILAMGRQNKMTGAAEQYQYILRDESLHCNFGIDLINQIKLENPHLWTAEF
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHCCCCCCEEHHHH
KAEITELFKKAVDLEYRYAEDTMPRGVLGLNAPMFKGYLRFICNRRCQQIGLDQLFPNEE
HHHHHHHHHHHHCCCEEECCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCC
NPFPWMSEMIDLKKERNFFETRVIEYQTGGALSWD
CCCCHHHHHHHHHHHCCHHHHHEEEEECCCCCCCC
>Mature Secondary Structure
MLSWDDDVQATPQAAPQPALQPAAVATSVDQQGVLPPAATEPGILGNNPNAAAAQSTRRV
CCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHC
NAADKRVINGATDVNQLVPFKYKWAWEKYLAGCANHWMPQEINMSRDIALWKDPNGLTED
CHHHHHHHCCCCCHHHHCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHH
ERRIIKRNLGFFVTADSLAANNIVLGTYRQITAPECRQYLLRQAFEEAIHTHAYQYIVES
HHHHHHHCCCEEEEECCCCCCCEEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGLNEAEIFNAYHEVQSIRDKDEFLIPFIDTLTDPSFKTGTPENDQKLLKSLIVFACIME
HCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
GLFFYVGFTQILAMGRQNKMTGAAEQYQYILRDESLHCNFGIDLINQIKLENPHLWTAEF
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHCCCCCCEEHHHH
KAEITELFKKAVDLEYRYAEDTMPRGVLGLNAPMFKGYLRFICNRRCQQIGLDQLFPNEE
HHHHHHHHHHHHCCCEEECCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCC
NPFPWMSEMIDLKKERNFFETRVIEYQTGGALSWD
CCCCHHHHHHHHHHHCCHHHHHEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]