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The map label for this gene is traF [H]

Identifier: 94311952

GI number: 94311952

Start: 3279450

End: 3280037

Strand: Reverse

Name: traF [H]

Synonym: Rmet_3020

Alternate gene names: 94311952

Gene position: 3280037-3279450 (Counterclockwise)

Preceding gene: 94311953

Following gene: 94311951

Centisome position: 83.5

GC content: 69.73

Gene sequence:

>588_bases
ATGACCGCAGTTTCCACTTCCGGCACCGCGCCGCACCCTCGCTTGCGCGTGCGCGCTCGCCTCGTGCTGGCGGGCCTGTC
CGCCTGCGGCCTCGCTGCGCTGGCCTGGGCGTCCTTCGTGCATCCGCTGCCACGCCTGACCTACAACCCGTCCGACAGCG
TGGAGGTCGGCTGGTATCGCATCGATCCGCTCGACCATCGCACCAGCTCGCCACCACGTCCGTTGTCGGTAGGCAGCATC
GTGCTGGTGCCGCTGCCTGCCGAGGCTGCCGCGCTCGCTGCGCAGCGCGGCTACCTGCCGACGCGCATTCCTCTGCTCAA
GCGCGTGGGCGCGGTGGCGCCGCAAGAGGTGTGCATCGCTGACGGCAAAGTCCGCATCGACGGCGTGCCTTCAGCCGCCG
TGCTGTCTGCCGATCGCTGGGGCCGGCCGCTGCCATCCCGGCAGCAATGCCACCGCTTGCGGCATGGCGAGCTGTTCCTC
CTCAGTGTGACCAATCCGGCGTCGTTCGATAGCCGGTATTTCGGGCCGGTCAGCGCAGCTTCCGTGATCGGCGTTGCGCG
CCCCGTCTGGCTGGAGTCGCGCCCATGA

Upstream 100 bases:

>100_bases
CGTACTGGCGCACGCTCGCCAACCGGCTCGCAGCGCACTTGCCGCTGCCCGAATACACCGCCGAGCGGCACGCCGCCTGG
CTGGCCGGGAGGGCGCTGCC

Downstream 100 bases:

>100_bases
TGGCCACCGATTCGCTGCGCATCATCGTGCTATCGGGCGTGTCGTTCTGCTGGCCGTTGTCATGTCGTCGCGCGTGCAGT
GCGAGCGCATCCGCGCTGCA

Product: plasmid conjugal transfer transmembrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MTAVSTSGTAPHPRLRVRARLVLAGLSACGLAALAWASFVHPLPRLTYNPSDSVEVGWYRIDPLDHRTSSPPRPLSVGSI
VLVPLPAEAAALAAQRGYLPTRIPLLKRVGAVAPQEVCIADGKVRIDGVPSAAVLSADRWGRPLPSRQQCHRLRHGELFL
LSVTNPASFDSRYFGPVSAASVIGVARPVWLESRP

Sequences:

>Translated_195_residues
MTAVSTSGTAPHPRLRVRARLVLAGLSACGLAALAWASFVHPLPRLTYNPSDSVEVGWYRIDPLDHRTSSPPRPLSVGSI
VLVPLPAEAAALAAQRGYLPTRIPLLKRVGAVAPQEVCIADGKVRIDGVPSAAVLSADRWGRPLPSRQQCHRLRHGELFL
LSVTNPASFDSRYFGPVSAASVIGVARPVWLESRP
>Mature_194_residues
TAVSTSGTAPHPRLRVRARLVLAGLSACGLAALAWASFVHPLPRLTYNPSDSVEVGWYRIDPLDHRTSSPPRPLSVGSIV
LVPLPAEAAALAAQRGYLPTRIPLLKRVGAVAPQEVCIADGKVRIDGVPSAAVLSADRWGRPLPSRQQCHRLRHGELFLL
SVTNPASFDSRYFGPVSAASVIGVARPVWLESRP

Specific function: Required for donor-specific phage sensitivity. May be involved in pilus assembly [H]

COG id: COG4959

COG function: function code OU; Type IV secretory pathway, protease TraF

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26C family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR019533
- InterPro:   IPR014139 [H]

Pfam domain/function: PF10502 Peptidase_S26 [H]

EC number: NA

Molecular weight: Translated: 20887; Mature: 20756

Theoretical pI: Translated: 11.18; Mature: 11.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAVSTSGTAPHPRLRVRARLVLAGLSACGLAALAWASFVHPLPRLTYNPSDSVEVGWYR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCEEEEEEE
IDPLDHRTSSPPRPLSVGSIVLVPLPAEAAALAAQRGYLPTRIPLLKRVGAVAPQEVCIA
ECCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEE
DGKVRIDGVPSAAVLSADRWGRPLPSRQQCHRLRHGELFLLSVTNPASFDSRYFGPVSAA
CCEEEECCCCCHHEEECHHCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHH
SVIGVARPVWLESRP
HHHHHCCCEEECCCC
>Mature Secondary Structure 
TAVSTSGTAPHPRLRVRARLVLAGLSACGLAALAWASFVHPLPRLTYNPSDSVEVGWYR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCEEEEEEE
IDPLDHRTSSPPRPLSVGSIVLVPLPAEAAALAAQRGYLPTRIPLLKRVGAVAPQEVCIA
ECCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEE
DGKVRIDGVPSAAVLSADRWGRPLPSRQQCHRLRHGELFLLSVTNPASFDSRYFGPVSAA
CCEEEECCCCCHHEEECHHCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHH
SVIGVARPVWLESRP
HHHHHCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400217 [H]