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The map label for this gene is rsmE [H]

Identifier: 94311915

GI number: 94311915

Start: 3242340

End: 3243083

Strand: Direct

Name: rsmE [H]

Synonym: Rmet_2983

Alternate gene names: 94311915

Gene position: 3242340-3243083 (Clockwise)

Preceding gene: 94311914

Following gene: 94311916

Centisome position: 82.54

GC content: 67.07

Gene sequence:

>744_bases
ATGCCACCCAGATTTTTTGTCGATGCGGCGCTCGCCGCGGACACCGTATTGGAATTACCCGACGCCGTCGTGCGCCATGT
CCAGGTGTTGCGCCTGGCGCCCGGTGACGCCATCACCCTGTTCAACGGTCAGGGCGGGAGCCACCCGGCCACGCTGACCG
AGATCGGCAAGCGCCACGCTAGTGCGACGCTTGGCACCCACGACGCCTCGGAAGCCGAAACCCCGTTCGCCGTCACGCTC
GCCCAGGGGCTTGCCGGGGGCGACAAGATGGACTGGCTGATCGAGAAGGCAGTGGAACTCGGTGTATCCGCCATCCAGCC
ACTTCAGGCTGCCAGATCCGTCGTCCGGCTTTCCGGCGAGCGCGCCACCAAGCGCCAGGCGCACTGGCAGGCACTGATCG
AGGCCGCCTGCGAACAATGCGGCCGTAATCGCTTGCCGGCGTTGGCGCCCGTGGCTAACTTGGAGTCATGGCTGACCAGC
GTGCCGGCCACTGCTGCAAGGCTGGCGCTCTCGCCACGCGCATCGGCATCGCTGCCCGAACTGGCGCAAGCCGAGCGCGA
GGCATGGCGCGCCGGCGGCGTGACGTTGCTGATCGGGCCCGAGGGCGGCTTGTCGCCGGAAGAAGAAGATCGCGCACGCC
GAGCCGGTTTTATCGGCGTGTCATTGGGACCGCGCATTCTGCGAACGGAAACCGCCGGGCTGACCTGCCTGGCGACACTG
AACGCCATCCTTGGCGGGTTTTGA

Upstream 100 bases:

>100_bases
TCTGGGGCGACCGGTACTGCATGGTCGAAGATCCCGATGGCTACCGCTGGGGCTTTGCGACGCCGCTGCCCAAGACCCCG
GATTCCAAACCCACTCCCTG

Downstream 100 bases:

>100_bases
CCTAGAAGGAGTCAAACATGGGACTACTCGATAGCGTGCTGGGCGGTGTGCTCGGCCAACTGGGCGGCGGCCAGCACGGC
GAACAGGCCGGAGGCGGCCT

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MPPRFFVDAALAADTVLELPDAVVRHVQVLRLAPGDAITLFNGQGGSHPATLTEIGKRHASATLGTHDASEAETPFAVTL
AQGLAGGDKMDWLIEKAVELGVSAIQPLQAARSVVRLSGERATKRQAHWQALIEAACEQCGRNRLPALAPVANLESWLTS
VPATAARLALSPRASASLPELAQAEREAWRAGGVTLLIGPEGGLSPEEEDRARRAGFIGVSLGPRILRTETAGLTCLATL
NAILGGF

Sequences:

>Translated_247_residues
MPPRFFVDAALAADTVLELPDAVVRHVQVLRLAPGDAITLFNGQGGSHPATLTEIGKRHASATLGTHDASEAETPFAVTL
AQGLAGGDKMDWLIEKAVELGVSAIQPLQAARSVVRLSGERATKRQAHWQALIEAACEQCGRNRLPALAPVANLESWLTS
VPATAARLALSPRASASLPELAQAEREAWRAGGVTLLIGPEGGLSPEEEDRARRAGFIGVSLGPRILRTETAGLTCLATL
NAILGGF
>Mature_246_residues
PPRFFVDAALAADTVLELPDAVVRHVQVLRLAPGDAITLFNGQGGSHPATLTEIGKRHASATLGTHDASEAETPFAVTLA
QGLAGGDKMDWLIEKAVELGVSAIQPLQAARSVVRLSGERATKRQAHWQALIEAACEQCGRNRLPALAPVANLESWLTSV
PATAARLALSPRASASLPELAQAEREAWRAGGVTLLIGPEGGLSPEEEDRARRAGFIGVSLGPRILRTETAGLTCLATLN
AILGGF

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=247, Percent_Identity=38.0566801619433, Blast_Score=161, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 25869; Mature: 25738

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPRFFVDAALAADTVLELPDAVVRHVQVLRLAPGDAITLFNGQGGSHPATLTEIGKRHA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHH
SATLGTHDASEAETPFAVTLAQGLAGGDKMDWLIEKAVELGVSAIQPLQAARSVVRLSGE
HHHCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RATKRQAHWQALIEAACEQCGRNRLPALAPVANLESWLTSVPATAARLALSPRASASLPE
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCHH
LAQAEREAWRAGGVTLLIGPEGGLSPEEEDRARRAGFIGVSLGPRILRTETAGLTCLATL
HHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHCCEEEECCCCHHEEECCCCHHHHHHH
NAILGGF
HHHHCCC
>Mature Secondary Structure 
PPRFFVDAALAADTVLELPDAVVRHVQVLRLAPGDAITLFNGQGGSHPATLTEIGKRHA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHH
SATLGTHDASEAETPFAVTLAQGLAGGDKMDWLIEKAVELGVSAIQPLQAARSVVRLSGE
HHHCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RATKRQAHWQALIEAACEQCGRNRLPALAPVANLESWLTSVPATAARLALSPRASASLPE
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCHH
LAQAEREAWRAGGVTLLIGPEGGLSPEEEDRARRAGFIGVSLGPRILRTETAGLTCLATL
HHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHCCEEEECCCCHHEEECCCCHHHHHHH
NAILGGF
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8332061 [H]