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The map label for this gene is speE [H]

Identifier: 94311913

GI number: 94311913

Start: 3240866

End: 3241840

Strand: Direct

Name: speE [H]

Synonym: Rmet_2981

Alternate gene names: 94311913

Gene position: 3240866-3241840 (Clockwise)

Preceding gene: 94311910

Following gene: 94311914

Centisome position: 82.5

GC content: 68.0

Gene sequence:

>975_bases
ATGCGAGAACCTGACGGCGGGAAAACCGCAATTTTAGCAGAAGCAGGGGGAATTCCCGGGACGGATCCGGCACTCCGAAG
GGGGACTCAGGCCTGGACCGAATGGTTGATACCCGGCGTCGGCACCACGCTAGGCGAAATCACATTGCTAGCGGCCGCGT
GCAGCCCCTATCAGCAGATAGAAATCGGGGAAAGCGCACATTTTGGCCGGATCTTCCGGCTCGATGGCCGCGTGATGAGC
ACCGAAGCCGACGCCTGGATCCAGCACGAGCTGATGGTGGCCCCAATGGCGGCCACCCATGGCGCGCCGCGCAGCGCGCT
GGTGGTGGGCGGCGGCGACGGCGGATCGGCATTCGAACTGCTGCGTATCCCCACCATGCGAGAGGTAGTGGTGGCCGAAC
TCGACGGCGAGGTGGTGCGGCTGGCGCGCGAGTGGTTGCGCGGCATCCACCAGGGCGCGCTCGACGAACCAGGCGACCCG
CGCCTGAGCCTGGCGATCGGCGAAGGACTCGGCCTGATGGAAGCCTTCGCGCGCGCCGGACGCCAGTTCGACCTGATCGT
CTATGACCTGACCGAAGCTGACGACGGCAGCCCCGCCGCCGCCCTGTTCTCCCCGCATGGCCTGCATACCGCGCGCCGCT
GCCTGGCGCCCGGCGGCGCGATGTCGCTGCACCTGGGGCCCCCGCTGCATCGGCCGGAAACGGCGCGGCGGCTGCTGGCA
CGGCTACGGCATGTGTTCCGGCATGTCGCGCCACTGACGACCTTCGTCCCGGCCTATGGCGCGCTGTGGGGCATTGCGCT
GGCCAGCGACGCGCTCGATATCGCCGCGCAGCCCGCTGACAAGATCGCAACGCGTCTGCAGCAGTGGCAACTCGAACACA
AGCTGCGGGCCTATCACGCGTACCTGCACCCTGCCCTGTTCGTCCATCCGCGGCACGTGCAGGCGATCTTCGACAGCGGC
AGCCGTCCCGCGTAA

Upstream 100 bases:

>100_bases
TTGCTGGACAGCGTCCATGGCAAGCACGCGGATGGCATCCGCCATCAGTTTGACGGGTTGGTTGGCGTAAGGACTTGTGG
CGGGATGGGCGAGGGCAGAC

Downstream 100 bases:

>100_bases
CATGCACGGACGCGCCTCGCGCCACGCAGCGCGGGGACGAGATTCGTTCCATCCGGGAGATCATCATGTCCAGACCGCCC
AACACGCCCTGGCTTACGCC

Product: Spermidine synthase (Putrescine aminopropyltransferase) (SPDSY)

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MREPDGGKTAILAEAGGIPGTDPALRRGTQAWTEWLIPGVGTTLGEITLLAAACSPYQQIEIGESAHFGRIFRLDGRVMS
TEADAWIQHELMVAPMAATHGAPRSALVVGGGDGGSAFELLRIPTMREVVVAELDGEVVRLAREWLRGIHQGALDEPGDP
RLSLAIGEGLGLMEAFARAGRQFDLIVYDLTEADDGSPAAALFSPHGLHTARRCLAPGGAMSLHLGPPLHRPETARRLLA
RLRHVFRHVAPLTTFVPAYGALWGIALASDALDIAAQPADKIATRLQQWQLEHKLRAYHAYLHPALFVHPRHVQAIFDSG
SRPA

Sequences:

>Translated_324_residues
MREPDGGKTAILAEAGGIPGTDPALRRGTQAWTEWLIPGVGTTLGEITLLAAACSPYQQIEIGESAHFGRIFRLDGRVMS
TEADAWIQHELMVAPMAATHGAPRSALVVGGGDGGSAFELLRIPTMREVVVAELDGEVVRLAREWLRGIHQGALDEPGDP
RLSLAIGEGLGLMEAFARAGRQFDLIVYDLTEADDGSPAAALFSPHGLHTARRCLAPGGAMSLHLGPPLHRPETARRLLA
RLRHVFRHVAPLTTFVPAYGALWGIALASDALDIAAQPADKIATRLQQWQLEHKLRAYHAYLHPALFVHPRHVQAIFDSG
SRPA
>Mature_324_residues
MREPDGGKTAILAEAGGIPGTDPALRRGTQAWTEWLIPGVGTTLGEITLLAAACSPYQQIEIGESAHFGRIFRLDGRVMS
TEADAWIQHELMVAPMAATHGAPRSALVVGGGDGGSAFELLRIPTMREVVVAELDGEVVRLAREWLRGIHQGALDEPGDP
RLSLAIGEGLGLMEAFARAGRQFDLIVYDLTEADDGSPAAALFSPHGLHTARRCLAPGGAMSLHLGPPLHRPETARRLLA
RLRHVFRHVAPLTTFVPAYGALWGIALASDALDIAAQPADKIATRLQQWQLEHKLRAYHAYLHPALFVHPRHVQAIFDSG
SRPA

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=300, Percent_Identity=27.3333333333333, Blast_Score=101, Evalue=8e-22,
Organism=Escherichia coli, GI1786312, Length=286, Percent_Identity=30.0699300699301, Blast_Score=127, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI25154106, Length=282, Percent_Identity=31.2056737588652, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI212645616, Length=293, Percent_Identity=27.9863481228669, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323175, Length=282, Percent_Identity=27.3049645390071, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6325326, Length=280, Percent_Identity=24.6428571428571, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24645443, Length=283, Percent_Identity=28.2685512367491, Blast_Score=104, Evalue=8e-23,
Organism=Drosophila melanogaster, GI21355941, Length=224, Percent_Identity=29.0178571428571, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 34871; Mature: 34871

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREPDGGKTAILAEAGGIPGTDPALRRGTQAWTEWLIPGVGTTLGEITLLAAACSPYQQI
CCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHEE
EIGESAHFGRIFRLDGRVMSTEADAWIQHELMVAPMAATHGAPRSALVVGGGDGGSAFEL
ECCCCCCCCEEEEECCEEEECCCHHHHHHHHHEEEHHHHCCCCCCEEEEECCCCCCCCCE
LRIPTMREVVVAELDGEVVRLAREWLRGIHQGALDEPGDPRLSLAIGEGLGLMEAFARAG
EECCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHCC
RQFDLIVYDLTEADDGSPAAALFSPHGLHTARRCLAPGGAMSLHLGPPLHRPETARRLLA
CEEEEEEEEECCCCCCCCCEECCCCCCHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHH
RLRHVFRHVAPLTTFVPAYGALWGIALASDALDIAAQPADKIATRLQQWQLEHKLRAYHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
YLHPALFVHPRHVQAIFDSGSRPA
HHCHHHEECHHHHHHHHCCCCCCC
>Mature Secondary Structure
MREPDGGKTAILAEAGGIPGTDPALRRGTQAWTEWLIPGVGTTLGEITLLAAACSPYQQI
CCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHEE
EIGESAHFGRIFRLDGRVMSTEADAWIQHELMVAPMAATHGAPRSALVVGGGDGGSAFEL
ECCCCCCCCEEEEECCEEEECCCHHHHHHHHHEEEHHHHCCCCCCEEEEECCCCCCCCCE
LRIPTMREVVVAELDGEVVRLAREWLRGIHQGALDEPGDPRLSLAIGEGLGLMEAFARAG
EECCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHCC
RQFDLIVYDLTEADDGSPAAALFSPHGLHTARRCLAPGGAMSLHLGPPLHRPETARRLLA
CEEEEEEEEECCCCCCCCCEECCCCCCHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHH
RLRHVFRHVAPLTTFVPAYGALWGIALASDALDIAAQPADKIATRLQQWQLEHKLRAYHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
YLHPALFVHPRHVQAIFDSGSRPA
HHCHHHEECHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]