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The map label for this gene is ycbY [H]

Identifier: 94311899

GI number: 94311899

Start: 3225270

End: 3226529

Strand: Reverse

Name: ycbY [H]

Synonym: Rmet_2967

Alternate gene names: 94311899

Gene position: 3226529-3225270 (Counterclockwise)

Preceding gene: 94311900

Following gene: 335055639

Centisome position: 82.14

GC content: 66.27

Gene sequence:

>1260_bases
ATGACCCAAGCTTTCTTTGTTCCTTGCCCGCGCGGCCTGGAACCCGCCCTTGCCGAGGAACTACGCGAAATCGCGCGGTA
TCCGGCCGTGGTGGCCGCCGCGCCGTTCGAAGTGCATCGGGAGGTGCCGGGCGGTGTGACGTTCTCGGGGGAGATGGCGG
CCGCCTATGCCGTCAACCTCCACTCGCGCATCGCCAGCCGTGTGCTGCTGCGCGTGGCGCAGCGTGGATACCGGCACGAG
GACGACATCTACTCGCTGGTGCGCCAGCAGCGCTGGGAACAGTGGTTCTCGCCAGACGAGACGCTGCGTGTCGATGTCAC
GGCGCACAAGTCGCCGCTGCGCAGCCTGAATTTCACGGCGCTGCGCGTCAAGGACGGCGTCTGCGACGCAATGCGCGAGC
GGCTGGGTGCGCGTCCCAGCGTCGATACGGTGAGCCCGGATGTCCGCATCTACGCGCACCTGACCGAAACCGATTGCACC
GTCTATCTCGACACCACCGGCGAGCCGCTGTTCAAGCGCGGCTGGCGTATGGAAAAGGGCGAGGCGCCGCTCAAGGAGAA
CCTGGCCGCCGGCATTCTGCGTCTGGCCGGCTGGACGGCGGGTCAGACCGATCGTCCGTTCTTCGATCCGATGTGCGGCA
GCGGCACGTTCCTCGTCGAGGCCGCGCAGGTGGCGCTGGGCCTCGCGCCGGGCGCCGCTCGCACATTTGCGTTCGAGTGG
CTGAAGGGCTGCGACACCAAGGCATGGCAGGCGCTGCGTGCCGATGCACAGCGCGCGCGTGAGCAAGCTGCCGGCCGCGT
CACGAATCTGAAGATCGCCGGGTCGGATATTTCCACGGACATGCTGTCGATGACCCAGGCCAACTGGCAACGCGCCGGCC
TGCCGGGCGATGTGCAACTGAAACAGGTGGATGCGCGCTTCGTCCAGCCGCCTTTTGCCGACGCAGGGCTAATGCTGATG
AACCCGCCCTACGGCGAGCGGATTGCCGTGCGTGGCGATCGCCGGGGAGCCATGGAGGACGCGCCACGCGACGAGGCCGA
GGAAGCCGCCGCCAACCAGTTCGCCGCGGCGTTTGCGACGACGCTGAAGCAGAGTTTCTCGGGCTGGCAGGCGTGGGTAT
TCACTGGCGATCTGACGATGCCGAAGCGACTACGCCTGAAAGAATCGCGCCGCACGCCGCTCTACAACGGCAATATCGAG
TGCAGGTTGTTCCGGTTCGATATGGTGCGGGGCGGGAATCGGGAACAGAAGAAGGATTGA

Upstream 100 bases:

>100_bases
ATTTCCTCGACCGCGCGCTCGCAGCGGGCTGATCGGGGGACGGTCACCGATACCTTCCGTCCGCCATGGCGGACGTTTTT
GTTTGCAGGATTTTCACGCC

Downstream 100 bases:

>100_bases
GCGATGGCTCTAAGCCTCTCTCCCGCGTGTGGGGGAGGGGCGACAACAATTGGTGCTTCCAATGTTGTCGAATCAGTCGC
GCTTGTTCCGCCCCGTCATC

Product: AdoMet-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MTQAFFVPCPRGLEPALAEELREIARYPAVVAAAPFEVHREVPGGVTFSGEMAAAYAVNLHSRIASRVLLRVAQRGYRHE
DDIYSLVRQQRWEQWFSPDETLRVDVTAHKSPLRSLNFTALRVKDGVCDAMRERLGARPSVDTVSPDVRIYAHLTETDCT
VYLDTTGEPLFKRGWRMEKGEAPLKENLAAGILRLAGWTAGQTDRPFFDPMCGSGTFLVEAAQVALGLAPGAARTFAFEW
LKGCDTKAWQALRADAQRAREQAAGRVTNLKIAGSDISTDMLSMTQANWQRAGLPGDVQLKQVDARFVQPPFADAGLMLM
NPPYGERIAVRGDRRGAMEDAPRDEAEEAAANQFAAAFATTLKQSFSGWQAWVFTGDLTMPKRLRLKESRRTPLYNGNIE
CRLFRFDMVRGGNREQKKD

Sequences:

>Translated_419_residues
MTQAFFVPCPRGLEPALAEELREIARYPAVVAAAPFEVHREVPGGVTFSGEMAAAYAVNLHSRIASRVLLRVAQRGYRHE
DDIYSLVRQQRWEQWFSPDETLRVDVTAHKSPLRSLNFTALRVKDGVCDAMRERLGARPSVDTVSPDVRIYAHLTETDCT
VYLDTTGEPLFKRGWRMEKGEAPLKENLAAGILRLAGWTAGQTDRPFFDPMCGSGTFLVEAAQVALGLAPGAARTFAFEW
LKGCDTKAWQALRADAQRAREQAAGRVTNLKIAGSDISTDMLSMTQANWQRAGLPGDVQLKQVDARFVQPPFADAGLMLM
NPPYGERIAVRGDRRGAMEDAPRDEAEEAAANQFAAAFATTLKQSFSGWQAWVFTGDLTMPKRLRLKESRRTPLYNGNIE
CRLFRFDMVRGGNREQKKD
>Mature_418_residues
TQAFFVPCPRGLEPALAEELREIARYPAVVAAAPFEVHREVPGGVTFSGEMAAAYAVNLHSRIASRVLLRVAQRGYRHED
DIYSLVRQQRWEQWFSPDETLRVDVTAHKSPLRSLNFTALRVKDGVCDAMRERLGARPSVDTVSPDVRIYAHLTETDCTV
YLDTTGEPLFKRGWRMEKGEAPLKENLAAGILRLAGWTAGQTDRPFFDPMCGSGTFLVEAAQVALGLAPGAARTFAFEWL
KGCDTKAWQALRADAQRAREQAAGRVTNLKIAGSDISTDMLSMTQANWQRAGLPGDVQLKQVDARFVQPPFADAGLMLMN
PPYGERIAVRGDRRGAMEDAPRDEAEEAAANQFAAAFATTLKQSFSGWQAWVFTGDLTMPKRLRLKESRRTPLYNGNIEC
RLFRFDMVRGGNREQKKD

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=364, Percent_Identity=31.5934065934066, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 46687; Mature: 46555

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAFFVPCPRGLEPALAEELREIARYPAVVAAAPFEVHREVPGGVTFSGEMAAAYAVNL
CCCEEECCCCCCCCHHHHHHHHHHHHCCEEEEECCHHHHHCCCCCEEECCCHHHHHHHHH
HSRIASRVLLRVAQRGYRHEDDIYSLVRQQRWEQWFSPDETLRVDVTAHKSPLRSLNFTA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHCCEEE
LRVKDGVCDAMRERLGARPSVDTVSPDVRIYAHLTETDCTVYLDTTGEPLFKRGWRMEKG
EEECCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCHHHHCCCCCCCC
EAPLKENLAAGILRLAGWTAGQTDRPFFDPMCGSGTFLVEAAQVALGLAPGAARTFAFEW
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHCCCCCHHHHHHHHH
LKGCDTKAWQALRADAQRAREQAAGRVTNLKIAGSDISTDMLSMTQANWQRAGLPGDVQL
HHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEE
KQVDARFVQPPFADAGLMLMNPPYGERIAVRGDRRGAMEDAPRDEAEEAAANQFAAAFAT
EEHHHHHCCCCCCCCCEEEECCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHH
TLKQSFSGWQAWVFTGDLTMPKRLRLKESRRTPLYNGNIECRLFRFDMVRGGNREQKKD
HHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
TQAFFVPCPRGLEPALAEELREIARYPAVVAAAPFEVHREVPGGVTFSGEMAAAYAVNL
CCEEECCCCCCCCHHHHHHHHHHHHCCEEEEECCHHHHHCCCCCEEECCCHHHHHHHHH
HSRIASRVLLRVAQRGYRHEDDIYSLVRQQRWEQWFSPDETLRVDVTAHKSPLRSLNFTA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHCCEEE
LRVKDGVCDAMRERLGARPSVDTVSPDVRIYAHLTETDCTVYLDTTGEPLFKRGWRMEKG
EEECCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCHHHHCCCCCCCC
EAPLKENLAAGILRLAGWTAGQTDRPFFDPMCGSGTFLVEAAQVALGLAPGAARTFAFEW
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHCCCCCHHHHHHHHH
LKGCDTKAWQALRADAQRAREQAAGRVTNLKIAGSDISTDMLSMTQANWQRAGLPGDVQL
HHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEE
KQVDARFVQPPFADAGLMLMNPPYGERIAVRGDRRGAMEDAPRDEAEEAAANQFAAAFAT
EEHHHHHCCCCCCCCCEEEECCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHH
TLKQSFSGWQAWVFTGDLTMPKRLRLKESRRTPLYNGNIECRLFRFDMVRGGNREQKKD
HHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA