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The map label for this gene is pntB [H]

Identifier: 94311897

GI number: 94311897

Start: 3222459

End: 3223931

Strand: Direct

Name: pntB [H]

Synonym: Rmet_2965

Alternate gene names: 94311897

Gene position: 3222459-3223931 (Clockwise)

Preceding gene: 94311896

Following gene: 94311898

Centisome position: 82.04

GC content: 63.41

Gene sequence:

>1473_bases
ATGGAAGTCATCAGCATGAACCTTGTGACGCTGCTGTACCTGGTGGCATCGGTCTGCTTCATCCAGGCGCTGAAAGGCCT
CTCTCACCCGGCATCGGCCCGCAAGGGCAATGCGTTCGGCATGATCGGCATGGCCATCGCCGTGGTGACCACGCTGGCGC
TGATCGTCAAGATCAAGCACGAGTTCCTGGCGGTAGGCGCGGCGCAGTCGTCGGTTGGTACGGGTCTGGCGCTGATCTTC
GGCGCGCTGGTGGTCGGCGGCGGCATCGGCGCCTATGTGGCGAAGAAGGTCGAAATGACCAAGATGCCCGAGCTGGTGGC
CGCGATGCACTCGCTGATCGGTCTGGCCGCCGTGTTCATCGCCGTGGCCGCCGTGGCGGAGCCCGCCGCGTTCGGCATCA
CGCCGGCTGGCTCGCACCTGATCCCGCTCGGCAACCGCATCGAACTGTTCATCGGCTGCTTCGTCGGTGCGATTACGTTC
TCCGGTTCGGTGATCGCATTCGGCAAGCTGTCTGGCCGCTACAAGTTCCGGCTGTTCCAGGGCGCGCCGGTGGTGTTCCC
GGGCCAGCACTGGCTGAACCTGCTGCTGGCCATCGGCATGATCGGCTTCGGCGTGATCTTCTTCCTGTCGCAGTCGTGGC
TGCCGTTCCTGATCATGCTGGCCATCGCGTTCGTGCTTGGCGTGCTGATCATCATTCCGATCGGCGGCGCCGACATGCCC
GTGGTGGTGTCGATGCTGAACTCGTACTCGGGCTGGGCAGCGGCCGGCATCGGCTTCTCGCTGAACAACCCGATGCTGAT
CATCGCGGGCTCGCTGGTGGGCTCATCCGGCGCGATCCTCTCGTACATCATGTGCAAGGCGATGAACCGCTCGTTCTTCA
ACGTGATCCTGGGCGGCTTCGGCGCGGATGCATCGGCCGGCGCAACGGCGGGCGCGCAGGCGCAGCGCAATGTGAAGTCC
GGATCGGCGGATGACGCGGCCTTCGTGATGAGCAATGCGGAAACCGTGATCATCGTGCCCGGCTACGGCCTGGCTGTGGC
CCGTGCCCAGCATGCGCTTAAAGAATTGACCGAGAAGCTGACCGAGCACGGCGTGACGGTGAAGTATGCGATCCACCCCG
TGGCGGGCCGGATGCCAGGTCACATGAACGTGCTGCTGGCCGAAGCCGAGGTGCCGTACGACCAGGTCTTCGAGATGGAG
GACATCAACAGCGAGTTTGGCCAGGCCGACGTGGTGCTGGTGCTAGGCGCCAACGACGTGGTCAATCCGGCCGCCAAGAC
CGACCCGAAATCGCCGATCGCCGGGATGCCGATACTGGAGGCGTACAAGGCCAAGACGATCATTGTGAACAAGCGTTCGA
TGGCAGCCGGCTACGCGGGTCTGGACAACGAACTGTTCTACATGGACAAGACGATGATGGTGTTCGGCGACGCCAAGAAG
GTGGTCGAGGACATGTTCAAGGCGGTCGACTAA

Upstream 100 bases:

>100_bases
CGGTGAACGTGTTCGGTGGCTTCCTGGTGACCCAGCGCATGCTGGAGATGTTCAAGAAGAAGGAACCGAAGGCGAAGGCT
GACAACAAGGGAGGTGCGTG

Downstream 100 bases:

>100_bases
TCTGCCGTTAAGCTTCCGAGCGGCGGCAGGTCCGCGAAGTGAATAAAACAACGCGCAGACCTGAAATCAACGAAATCGAA
CGAGTGCGGCCGGCAACCAA

Product: pyridine nucleotide transhydrogenase, subunit beta

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Proton-translocating transhydrogenase NADP(H)-binding component; Pyridine nucleotide transhydrogenase subunit beta; dIII [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MEVISMNLVTLLYLVASVCFIQALKGLSHPASARKGNAFGMIGMAIAVVTTLALIVKIKHEFLAVGAAQSSVGTGLALIF
GALVVGGGIGAYVAKKVEMTKMPELVAAMHSLIGLAAVFIAVAAVAEPAAFGITPAGSHLIPLGNRIELFIGCFVGAITF
SGSVIAFGKLSGRYKFRLFQGAPVVFPGQHWLNLLLAIGMIGFGVIFFLSQSWLPFLIMLAIAFVLGVLIIIPIGGADMP
VVVSMLNSYSGWAAAGIGFSLNNPMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVILGGFGADASAGATAGAQAQRNVKS
GSADDAAFVMSNAETVIIVPGYGLAVARAQHALKELTEKLTEHGVTVKYAIHPVAGRMPGHMNVLLAEAEVPYDQVFEME
DINSEFGQADVVLVLGANDVVNPAAKTDPKSPIAGMPILEAYKAKTIIVNKRSMAAGYAGLDNELFYMDKTMMVFGDAKK
VVEDMFKAVD

Sequences:

>Translated_490_residues
MEVISMNLVTLLYLVASVCFIQALKGLSHPASARKGNAFGMIGMAIAVVTTLALIVKIKHEFLAVGAAQSSVGTGLALIF
GALVVGGGIGAYVAKKVEMTKMPELVAAMHSLIGLAAVFIAVAAVAEPAAFGITPAGSHLIPLGNRIELFIGCFVGAITF
SGSVIAFGKLSGRYKFRLFQGAPVVFPGQHWLNLLLAIGMIGFGVIFFLSQSWLPFLIMLAIAFVLGVLIIIPIGGADMP
VVVSMLNSYSGWAAAGIGFSLNNPMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVILGGFGADASAGATAGAQAQRNVKS
GSADDAAFVMSNAETVIIVPGYGLAVARAQHALKELTEKLTEHGVTVKYAIHPVAGRMPGHMNVLLAEAEVPYDQVFEME
DINSEFGQADVVLVLGANDVVNPAAKTDPKSPIAGMPILEAYKAKTIIVNKRSMAAGYAGLDNELFYMDKTMMVFGDAKK
VVEDMFKAVD
>Mature_490_residues
MEVISMNLVTLLYLVASVCFIQALKGLSHPASARKGNAFGMIGMAIAVVTTLALIVKIKHEFLAVGAAQSSVGTGLALIF
GALVVGGGIGAYVAKKVEMTKMPELVAAMHSLIGLAAVFIAVAAVAEPAAFGITPAGSHLIPLGNRIELFIGCFVGAITF
SGSVIAFGKLSGRYKFRLFQGAPVVFPGQHWLNLLLAIGMIGFGVIFFLSQSWLPFLIMLAIAFVLGVLIIIPIGGADMP
VVVSMLNSYSGWAAAGIGFSLNNPMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVILGGFGADASAGATAGAQAQRNVKS
GSADDAAFVMSNAETVIIVPGYGLAVARAQHALKELTEKLTEHGVTVKYAIHPVAGRMPGHMNVLLAEAEVPYDQVFEME
DINSEFGQADVVLVLGANDVVNPAAKTDPKSPIAGMPILEAYKAKTIIVNKRSMAAGYAGLDNELFYMDKTMMVFGDAKK
VVEDMFKAVD

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=486, Percent_Identity=46.9135802469136, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI122939153, Length=486, Percent_Identity=46.9135802469136, Blast_Score=406, Evalue=1e-113,
Organism=Escherichia coli, GI1787886, Length=492, Percent_Identity=51.219512195122, Blast_Score=459, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17550456, Length=482, Percent_Identity=42.9460580912863, Blast_Score=363, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 51213; Mature: 51213

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVISMNLVTLLYLVASVCFIQALKGLSHPASARKGNAFGMIGMAIAVVTTLALIVKIKH
CCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EFLAVGAAQSSVGTGLALIFGALVVGGGIGAYVAKKVEMTKMPELVAAMHSLIGLAAVFI
HHHEECCCHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVAAVAEPAAFGITPAGSHLIPLGNRIELFIGCFVGAITFSGSVIAFGKLSGRYKFRLFQ
HHHHHHCCHHCCCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEC
GAPVVFPGQHWLNLLLAIGMIGFGVIFFLSQSWLPFLIMLAIAFVLGVLIIIPIGGADMP
CCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEEECCCCCHH
VVVSMLNSYSGWAAAGIGFSLNNPMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVILGGF
HHHHHHHCCCCCEEECCCEECCCCEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHCC
GADASAGATAGAQAQRNVKSGSADDAAFVMSNAETVIIVPGYGLAVARAQHALKELTEKL
CCCCCCCCCCCHHHHHHCCCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHH
TEHGVTVKYAIHPVAGRMPGHMNVLLAEAEVPYDQVFEMEDINSEFGQADVVLVLGANDV
HHCCCEEEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCEEEEECCCCC
VNPAAKTDPKSPIAGMPILEAYKAKTIIVNKRSMAAGYAGLDNELFYMDKTMMVFGDAKK
CCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCHHHCCCCCCCCCEEEECCEEEEECCHHH
VVEDMFKAVD
HHHHHHHHCC
>Mature Secondary Structure
MEVISMNLVTLLYLVASVCFIQALKGLSHPASARKGNAFGMIGMAIAVVTTLALIVKIKH
CCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EFLAVGAAQSSVGTGLALIFGALVVGGGIGAYVAKKVEMTKMPELVAAMHSLIGLAAVFI
HHHEECCCHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVAAVAEPAAFGITPAGSHLIPLGNRIELFIGCFVGAITFSGSVIAFGKLSGRYKFRLFQ
HHHHHHCCHHCCCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEC
GAPVVFPGQHWLNLLLAIGMIGFGVIFFLSQSWLPFLIMLAIAFVLGVLIIIPIGGADMP
CCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEEECCCCCHH
VVVSMLNSYSGWAAAGIGFSLNNPMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVILGGF
HHHHHHHCCCCCEEECCCEECCCCEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHCC
GADASAGATAGAQAQRNVKSGSADDAAFVMSNAETVIIVPGYGLAVARAQHALKELTEKL
CCCCCCCCCCCHHHHHHCCCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHH
TEHGVTVKYAIHPVAGRMPGHMNVLLAEAEVPYDQVFEMEDINSEFGQADVVLVLGANDV
HHCCCEEEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCEEEEECCCCC
VNPAAKTDPKSPIAGMPILEAYKAKTIIVNKRSMAAGYAGLDNELFYMDKTMMVFGDAKK
CCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCHHHCCCCCCCCCEEEECCEEEEECCHHH
VVEDMFKAVD
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]