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The map label for this gene is nadB [H]

Identifier: 94311804

GI number: 94311804

Start: 3121325

End: 3122932

Strand: Direct

Name: nadB [H]

Synonym: Rmet_2872

Alternate gene names: 94311804

Gene position: 3121325-3122932 (Clockwise)

Preceding gene: 94311803

Following gene: 94311811

Centisome position: 79.46

GC content: 65.67

Gene sequence:

>1608_bases
ATGAACTACGACGTCGCGATCGTCGGCAGCGGGCTGGCCGGCCTTACCGTCGCTTTGCAGCTTGCCGATACCCACCGGGT
TGTCATCCTCAGCAAACGCGCCATGACAACGGGGGCTAGCGATTGGGCCCAGGGCGGAATCGCCGCGGTGCTCGACTCGG
GCGATAGTCACGACGAACACACGCAGGACACGCTGGTTGCAGGTGCAGGACTTTGCGACGAGGATGCGACGCGCTTCATC
GTGGAGCATGGCCGCGAAGCGATCCAATGGCTGATCGACCGCGGGGTGCCGTTCACGCGCGACGAACAGGCCGAACTCGG
CTTCCACCTCACCCGCGAAGGCGGGCATAGCCGTCGCCGCATCATCCACGTTGCCGACGCCACGGGCCATGCGGTGGTGA
CCACGCTGGCCGAGCAGGCCCGCCAGCATCCGAACATCACGATCCTCGAAGACCAGTTCGCAGTCGACCTGATCACGTCC
GCCAAGCTCGGCTTGCCCGGCAACCGTTGCTATGGCCTGTACGTGCTCGATGCGGAAACGGGCGAAGTGCGCACGCTCAC
AGCCACGCATACGGTACTGGCCGCCGGCGGCGCGGGCAAGGTCTACCTCTATACGACCAACCCCGATACGGCTACGGGCG
ACGGCATCGCCATGGCCTGGCGCGCGGGCTGCCGGGTGTCGAACATGGAATTCATCCAGTTCCACCCGACCTGCCTCTAT
CACCCCTACGCGAAGTCGTTCCTGATCTCGGAAGCCGTGCGCGGCGAAGGCGGCCTGCTGAGGCTGCCGGATGGCACGCG
CTTCATGCCCGAGCATGATGCCCGCGCCGAACTGGCGCCGCGTGACGTGGTAGCCCGCGCCATCGACTTCGAAATGAAGA
AGCGCGGCCTGGATTGCGTGTATCTCGACATCACGCACCAGCCCGTGTCGTTCCTCAAGGAACACTTCCCGACGATCTAC
GCCCGCTGCCTGGAACTCGGCATCGATATCGCACGCGAGCCGATTCCCGTGGTGCCGGCCGCGCACTACACCTGTGGTGG
CGTGGTCACCGATACGGCGGGCCGCACCGATCTCGGCAATCTCTACGCAGTTGGCGAGACGGGCTGCACGGGCCTGCACG
GCGCCAACCGCCTGGCCTCGAACTCGCTGCTCGAATGCATGGTGATCGGCCGTGCCGCGGCTGCGGACATTGCCTCGCAA
GGCAAGGCCGACGCGCCGGACATCACGCTGCCGGCCTGGGACGAGAGCCGCGTATCGGATGCCGACGAGGAAGTCGTCGT
CTCGCACAACTGGGATGAGCTGCGCCGCCTGATGTGGAACTACGTCGGCATCGTGCGCACCGACAAGCGGCTGGAGCGCG
CGCAGCACCGGATCGGCATGCTGCGCGAGGAGATTGCCGAGTACTACGCGAATTTCCGCGTTACTCGCGATCTGTTGGAA
TTGCGCAACCTGGTGGAAGTGGCGTCGCTGATCGTCGACAGCGCCTACTCGCGCCACGAAAGCCGTGGCCTGCACTACAG
CCGTGACTATCCGGAAACGCTGCCGAAGGCGCTGCCTACCGTGCTGCAACCGCTGACGGGCCGCGCGGCAAGCCGCCGGA
AGGTCTGA

Upstream 100 bases:

>100_bases
CCTTGCCAGCCAAGGGGAAGGTGTAACAGTCTGGTGCAGGTGGGTGTAGACTTGCGAGCTTTCCCCCAACGCTCTTTCGC
CCTCGCGCGATCCCTGCACC

Downstream 100 bases:

>100_bases
GCCTCCGGCGACAGCCCAGGACCAGGTTCAGCCGACGATGCGCAGCGAGTAATCGGTCGCGCGCACATCCTTGGTGAGGG
CGCCGACCGAGATCCGGTCG

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO 1; Quinolinate synthase B 1 [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MNYDVAIVGSGLAGLTVALQLADTHRVVILSKRAMTTGASDWAQGGIAAVLDSGDSHDEHTQDTLVAGAGLCDEDATRFI
VEHGREAIQWLIDRGVPFTRDEQAELGFHLTREGGHSRRRIIHVADATGHAVVTTLAEQARQHPNITILEDQFAVDLITS
AKLGLPGNRCYGLYVLDAETGEVRTLTATHTVLAAGGAGKVYLYTTNPDTATGDGIAMAWRAGCRVSNMEFIQFHPTCLY
HPYAKSFLISEAVRGEGGLLRLPDGTRFMPEHDARAELAPRDVVARAIDFEMKKRGLDCVYLDITHQPVSFLKEHFPTIY
ARCLELGIDIAREPIPVVPAAHYTCGGVVTDTAGRTDLGNLYAVGETGCTGLHGANRLASNSLLECMVIGRAAAADIASQ
GKADAPDITLPAWDESRVSDADEEVVVSHNWDELRRLMWNYVGIVRTDKRLERAQHRIGMLREEIAEYYANFRVTRDLLE
LRNLVEVASLIVDSAYSRHESRGLHYSRDYPETLPKALPTVLQPLTGRAASRRKV

Sequences:

>Translated_535_residues
MNYDVAIVGSGLAGLTVALQLADTHRVVILSKRAMTTGASDWAQGGIAAVLDSGDSHDEHTQDTLVAGAGLCDEDATRFI
VEHGREAIQWLIDRGVPFTRDEQAELGFHLTREGGHSRRRIIHVADATGHAVVTTLAEQARQHPNITILEDQFAVDLITS
AKLGLPGNRCYGLYVLDAETGEVRTLTATHTVLAAGGAGKVYLYTTNPDTATGDGIAMAWRAGCRVSNMEFIQFHPTCLY
HPYAKSFLISEAVRGEGGLLRLPDGTRFMPEHDARAELAPRDVVARAIDFEMKKRGLDCVYLDITHQPVSFLKEHFPTIY
ARCLELGIDIAREPIPVVPAAHYTCGGVVTDTAGRTDLGNLYAVGETGCTGLHGANRLASNSLLECMVIGRAAAADIASQ
GKADAPDITLPAWDESRVSDADEEVVVSHNWDELRRLMWNYVGIVRTDKRLERAQHRIGMLREEIAEYYANFRVTRDLLE
LRNLVEVASLIVDSAYSRHESRGLHYSRDYPETLPKALPTVLQPLTGRAASRRKV
>Mature_535_residues
MNYDVAIVGSGLAGLTVALQLADTHRVVILSKRAMTTGASDWAQGGIAAVLDSGDSHDEHTQDTLVAGAGLCDEDATRFI
VEHGREAIQWLIDRGVPFTRDEQAELGFHLTREGGHSRRRIIHVADATGHAVVTTLAEQARQHPNITILEDQFAVDLITS
AKLGLPGNRCYGLYVLDAETGEVRTLTATHTVLAAGGAGKVYLYTTNPDTATGDGIAMAWRAGCRVSNMEFIQFHPTCLY
HPYAKSFLISEAVRGEGGLLRLPDGTRFMPEHDARAELAPRDVVARAIDFEMKKRGLDCVYLDITHQPVSFLKEHFPTIY
ARCLELGIDIAREPIPVVPAAHYTCGGVVTDTAGRTDLGNLYAVGETGCTGLHGANRLASNSLLECMVIGRAAAADIASQ
GKADAPDITLPAWDESRVSDADEEVVVSHNWDELRRLMWNYVGIVRTDKRLERAQHRIGMLREEIAEYYANFRVTRDLLE
LRNLVEVASLIVDSAYSRHESRGLHYSRDYPETLPKALPTVLQPLTGRAASRRKV

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=505, Percent_Identity=33.8613861386139, Blast_Score=234, Evalue=2e-61,
Organism=Escherichia coli, GI1788928, Length=524, Percent_Identity=60.8778625954198, Blast_Score=654, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=524, Percent_Identity=33.3969465648855, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1786942, Length=557, Percent_Identity=34.8294434470377, Blast_Score=224, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI17550100, Length=552, Percent_Identity=32.6086956521739, Blast_Score=234, Evalue=7e-62,
Organism=Caenorhabditis elegans, GI17505833, Length=530, Percent_Identity=32.2641509433962, Blast_Score=232, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI71986328, Length=462, Percent_Identity=27.4891774891775, Blast_Score=101, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6322701, Length=553, Percent_Identity=34.5388788426763, Blast_Score=241, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6322416, Length=550, Percent_Identity=34, Blast_Score=232, Evalue=9e-62,
Organism=Saccharomyces cerevisiae, GI6322511, Length=485, Percent_Identity=28.659793814433, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320788, Length=479, Percent_Identity=27.5574112734864, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17137288, Length=540, Percent_Identity=34.4444444444444, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24655642, Length=540, Percent_Identity=34.4444444444444, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24655647, Length=540, Percent_Identity=34.4444444444444, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24663005, Length=525, Percent_Identity=33.3333333333333, Blast_Score=230, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 58610; Mature: 58610

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYDVAIVGSGLAGLTVALQLADTHRVVILSKRAMTTGASDWAQGGIAAVLDSGDSHDEH
CCCEEEEEECCCCCEEEEEEECCCCEEEEEECCCCCCCCCHHHCCCEEEEECCCCCCCCH
TQDTLVAGAGLCDEDATRFIVEHGREAIQWLIDRGVPFTRDEQAELGFHLTREGGHSRRR
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCEEEECCCCCCCEE
IIHVADATGHAVVTTLAEQARQHPNITILEDQFAVDLITSAKLGLPGNRCYGLYVLDAET
EEEEECCCCHHHHHHHHHHHHHCCCEEEEECCHHEEHHHHCCCCCCCCCEEEEEEEECCC
GEVRTLTATHTVLAAGGAGKVYLYTTNPDTATGDGIAMAWRAGCRVSNMEFIQFHPTCLY
CCEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCEEEEEECCCEEE
HPYAKSFLISEAVRGEGGLLRLPDGTRFMPEHDARAELAPRDVVARAIDFEMKKRGLDCV
CHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEE
YLDITHQPVSFLKEHFPTIYARCLELGIDIAREPIPVVPAAHYTCGGVVTDTAGRTDLGN
EEEECCCHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCC
LYAVGETGCTGLHGANRLASNSLLECMVIGRAAAADIASQGKADAPDITLPAWDESRVSD
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
ADEEVVVSHNWDELRRLMWNYVGIVRTDKRLERAQHRIGMLREEIAEYYANFRVTRDLLE
CCCCEEEECCHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRNLVEVASLIVDSAYSRHESRGLHYSRDYPETLPKALPTVLQPLTGRAASRRKV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNYDVAIVGSGLAGLTVALQLADTHRVVILSKRAMTTGASDWAQGGIAAVLDSGDSHDEH
CCCEEEEEECCCCCEEEEEEECCCCEEEEEECCCCCCCCCHHHCCCEEEEECCCCCCCCH
TQDTLVAGAGLCDEDATRFIVEHGREAIQWLIDRGVPFTRDEQAELGFHLTREGGHSRRR
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCEEEECCCCCCCEE
IIHVADATGHAVVTTLAEQARQHPNITILEDQFAVDLITSAKLGLPGNRCYGLYVLDAET
EEEEECCCCHHHHHHHHHHHHHCCCEEEEECCHHEEHHHHCCCCCCCCCEEEEEEEECCC
GEVRTLTATHTVLAAGGAGKVYLYTTNPDTATGDGIAMAWRAGCRVSNMEFIQFHPTCLY
CCEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCEEEEEECCCEEE
HPYAKSFLISEAVRGEGGLLRLPDGTRFMPEHDARAELAPRDVVARAIDFEMKKRGLDCV
CHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEE
YLDITHQPVSFLKEHFPTIYARCLELGIDIAREPIPVVPAAHYTCGGVVTDTAGRTDLGN
EEEECCCHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCC
LYAVGETGCTGLHGANRLASNSLLECMVIGRAAAADIASQGKADAPDITLPAWDESRVSD
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
ADEEVVVSHNWDELRRLMWNYVGIVRTDKRLERAQHRIGMLREEIAEYYANFRVTRDLLE
CCCCEEEECCHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRNLVEVASLIVDSAYSRHESRGLHYSRDYPETLPKALPTVLQPLTGRAASRRKV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]