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The map label for this gene is cobS [H]

Identifier: 94311716

GI number: 94311716

Start: 3030870

End: 3031649

Strand: Reverse

Name: cobS [H]

Synonym: Rmet_2784

Alternate gene names: 94311716

Gene position: 3031649-3030870 (Counterclockwise)

Preceding gene: 94311717

Following gene: 94311715

Centisome position: 77.18

GC content: 66.28

Gene sequence:

>780_bases
ATGATGGCCAACGAACTCCGCTTCTTTCTCACTGCCGTCGGCTACTTCACGCGCGTGCCGTTGCCGGCGCGGCTGGCGAG
CTGGGTGGGGTTCGAGCCGCACTTCCTGAATGCCGCGTCGCGCTACTTCCCGCTGGTGGGGTTGATCGTTGGCGCCTTCG
GCGCGCTGGTGACATGGGGCGCGCTGTCGTTCTGGTCCCCGTCGGTGGCGATCGTGCTCGGCATGGCGGCCACGTTGCTG
TTCACCGGCGCGTTTCACGAGGATGGCCTGGCGGATTGCGTCGATGCCTTCGGTGGTGGCTATCGCCGCGAGGATGTGCT
GAGGATCATGCACGACTCGCGCGTGGGCGCGTTTGGCGCGATCGCCATTGTGGTGGCGCTGTTGCTGAAGTGGCAACTGC
TGGTATCGGTGGCGGAATCGGGCGGTGCGATGGTGCTGCTGGTGTTGCTGGTGGCGGCGCATGGAGCCAGCCGCGCGATG
GCGATCACGTTCCTGGCCACGCATGACTATGTGCGTGCCGAGGGCAAGGCCAAGCCGGTGGCGCAGCGCCTGTTTGGTGT
CGGACTGGTTTTCGCGCTGGCGTGCGGCGTCGTGCCGCTGTTGTGGCTGTCGCCGCTATTCGCGGGCGTGGCGATTCTGG
TGCTGGCCGTGGTGCGTGCCGCGCTGGGTGCGTACTTCGTGCGTCGTATCGGCGGCTATACCGGCGACTGCCTCGGTATG
GCGCAGCAGCTTGCCGAATTGTCGATCTACCTGGTGGCGGCGGCATGGAAGTGGTCCTGA

Upstream 100 bases:

>100_bases
CCGCTGGTGGCTTCGGCGGTGGCGTTCCTGCGGGAAATGGCCACGTTCAGCGGCGCGGGCGTCAGCAACGCGTCGGCGAC
CTGAAGCGACCTGAGGCGCC

Downstream 100 bases:

>100_bases
TCCGGCATGTCCGGCCCGATGTGGCGCCGGGCGTTTGCTACGGCGCGCTCGATCTGAAACTGGCGCAGCCGGTCAGTCCG
GCGCCGGAGCGGATCGTACG

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MMANELRFFLTAVGYFTRVPLPARLASWVGFEPHFLNAASRYFPLVGLIVGAFGALVTWGALSFWSPSVAIVLGMAATLL
FTGAFHEDGLADCVDAFGGGYRREDVLRIMHDSRVGAFGAIAIVVALLLKWQLLVSVAESGGAMVLLVLLVAAHGASRAM
AITFLATHDYVRAEGKAKPVAQRLFGVGLVFALACGVVPLLWLSPLFAGVAILVLAVVRAALGAYFVRRIGGYTGDCLGM
AQQLAELSIYLVAAAWKWS

Sequences:

>Translated_259_residues
MMANELRFFLTAVGYFTRVPLPARLASWVGFEPHFLNAASRYFPLVGLIVGAFGALVTWGALSFWSPSVAIVLGMAATLL
FTGAFHEDGLADCVDAFGGGYRREDVLRIMHDSRVGAFGAIAIVVALLLKWQLLVSVAESGGAMVLLVLLVAAHGASRAM
AITFLATHDYVRAEGKAKPVAQRLFGVGLVFALACGVVPLLWLSPLFAGVAILVLAVVRAALGAYFVRRIGGYTGDCLGM
AQQLAELSIYLVAAAWKWS
>Mature_259_residues
MMANELRFFLTAVGYFTRVPLPARLASWVGFEPHFLNAASRYFPLVGLIVGAFGALVTWGALSFWSPSVAIVLGMAATLL
FTGAFHEDGLADCVDAFGGGYRREDVLRIMHDSRVGAFGAIAIVVALLLKWQLLVSVAESGGAMVLLVLLVAAHGASRAM
AITFLATHDYVRAEGKAKPVAQRLFGVGLVFALACGVVPLLWLSPLFAGVAILVLAVVRAALGAYFVRRIGGYTGDCLGM
AQQLAELSIYLVAAAWKWS

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=248, Percent_Identity=32.6612903225806, Blast_Score=104, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 27549; Mature: 27549

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHEHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHEHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA