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The map label for this gene is fdhB [H]

Identifier: 94311692

GI number: 94311692

Start: 3000383

End: 3001063

Strand: Reverse

Name: fdhB [H]

Synonym: Rmet_2760

Alternate gene names: 94311692

Gene position: 3001063-3000383 (Counterclockwise)

Preceding gene: 94311693

Following gene: 94311691

Centisome position: 76.4

GC content: 66.81

Gene sequence:

>681_bases
ATGGCCCGCATGAAATTCATCTGTGATGCCGAGCGTTGCATCGAGTGCAACAGCTGCGTCACCGCCTGCAAGAACGAGCA
CGAGGTGCCCTGGGGTGTGAACCGGCGCCGCGTGGTGACGGTCAACGACGGCGTGGTCGGCGCGGAAAAGTCGATCTCGG
TGGCGTGCATGCATTGCTCCGATGCGCCGTGCATGGCGGTCTGTCCGGTCGATTGCTTCTATCGCACCGAGGACGGCGTG
GTGCTGCACGACAAGGATGTTTGCATCGGCTGCGGTTACTGCTCGTACGCCTGCCCATTCGGCGCGCCGCAGTTCCCTAG
CGAAGGTACCTTCGGCGTGCGCGGCAAGATGGACAAGTGCACGTTCTGCAACGGCGGCCCGGAAGCCAACGGCAGCGAGG
CAGAGTTCGAGAAGTACGGTCGCAACCGCCTGGCCGAAGGCAAGCTGCCGCTGTGCGCGGAAATGTGTTCGACCAAGGCG
CTGCTCGGCGGCGATGGCGACGTGATTGCCGACATCCTGCGCAATCGCGTGATCAAGCGCGGCAAGGGCGGCGACGTCTT
CGGCTGGGGCACCGCCTATGGCGGCGTCAAGGCGCAGCCGGCAGCGGGCACGCCGCCCGCGGCGCCGGGTGCCGCACCCA
CGGCGCCTGCCGCGCCAGCCAGCGAGGGGAGCGCATCATGA

Upstream 100 bases:

>100_bases
ACACCGCCACCACCTACGGCTATGACTCGGTGACGATGATGCAGGAAACCAAGACCACCGTTTGCCAGATCGAGCGGTTC
GCATAAGGACGGAGACGCAA

Downstream 100 bases:

>100_bases
ACCGCGCGCTACTGATTGCCCTGAGCCTTGCGGGCCTGCTGATCGCCGGCTGCGGCGAGAAGGCGCAGACATCGACCGCT
TCGTTCAAGAAATCGGATAC

Product: formate dehydrogenase iron-sulfur subunit

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MARMKFICDAERCIECNSCVTACKNEHEVPWGVNRRRVVTVNDGVVGAEKSISVACMHCSDAPCMAVCPVDCFYRTEDGV
VLHDKDVCIGCGYCSYACPFGAPQFPSEGTFGVRGKMDKCTFCNGGPEANGSEAEFEKYGRNRLAEGKLPLCAEMCSTKA
LLGGDGDVIADILRNRVIKRGKGGDVFGWGTAYGGVKAQPAAGTPPAAPGAAPTAPAAPASEGSAS

Sequences:

>Translated_226_residues
MARMKFICDAERCIECNSCVTACKNEHEVPWGVNRRRVVTVNDGVVGAEKSISVACMHCSDAPCMAVCPVDCFYRTEDGV
VLHDKDVCIGCGYCSYACPFGAPQFPSEGTFGVRGKMDKCTFCNGGPEANGSEAEFEKYGRNRLAEGKLPLCAEMCSTKA
LLGGDGDVIADILRNRVIKRGKGGDVFGWGTAYGGVKAQPAAGTPPAAPGAAPTAPAAPASEGSAS
>Mature_225_residues
ARMKFICDAERCIECNSCVTACKNEHEVPWGVNRRRVVTVNDGVVGAEKSISVACMHCSDAPCMAVCPVDCFYRTEDGVV
LHDKDVCIGCGYCSYACPFGAPQFPSEGTFGVRGKMDKCTFCNGGPEANGSEAEFEKYGRNRLAEGKLPLCAEMCSTKAL
LGGDGDVIADILRNRVIKRGKGGDVFGWGTAYGGVKAQPAAGTPPAAPGAAPTAPAAPASEGSAS

Specific function: This chain is an electron transfer unit containing 18 cysteine residues, 16 of which occur in four clusters [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787872, Length=169, Percent_Identity=36.094674556213, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787122, Length=191, Percent_Identity=34.0314136125654, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1789370, Length=202, Percent_Identity=29.7029702970297, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI226510944, Length=170, Percent_Identity=34.1176470588235, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI2367345, Length=189, Percent_Identity=31.2169312169312, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI87082180, Length=185, Percent_Identity=31.8918918918919, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1789079, Length=221, Percent_Identity=28.0542986425339, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082114, Length=90, Percent_Identity=35.5555555555556, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI2367245, Length=157, Percent_Identity=34.3949044585987, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790326, Length=192, Percent_Identity=26.0416666666667, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR000813 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.2.1.2

Molecular weight: Translated: 23589; Mature: 23458

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
10.6 %Cys+Met (Translated Protein)
8.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
10.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARMKFICDAERCIECNSCVTACKNEHEVPWGVNRRRVVTVNDGVVGAEKSISVACMHCS
CCCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCEECCCCCEEEEEEECC
DAPCMAVCPVDCFYRTEDGVVLHDKDVCIGCGYCSYACPFGAPQFPSEGTFGVRGKMDKC
CCCEEEECCCEEEEECCCCEEEECCCEEEECCCHHCCCCCCCCCCCCCCCCCCCCCCCCC
TFCNGGPEANGSEAEFEKYGRNRLAEGKLPLCAEMCSTKALLGGDGDVIADILRNRVIKR
EEECCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCEEECCCHHHHHHHHHHHHHHC
GKGGDVFGWGTAYGGVKAQPAAGTPPAAPGAAPTAPAAPASEGSAS
CCCCCEEECCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ARMKFICDAERCIECNSCVTACKNEHEVPWGVNRRRVVTVNDGVVGAEKSISVACMHCS
CCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCEECCCCCEEEEEEECC
DAPCMAVCPVDCFYRTEDGVVLHDKDVCIGCGYCSYACPFGAPQFPSEGTFGVRGKMDKC
CCCEEEECCCEEEEECCCCEEEECCCEEEECCCHHCCCCCCCCCCCCCCCCCCCCCCCCC
TFCNGGPEANGSEAEFEKYGRNRLAEGKLPLCAEMCSTKALLGGDGDVIADILRNRVIKR
EEECCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCEEECCCHHHHHHHHHHHHHHC
GKGGDVFGWGTAYGGVKAQPAAGTPPAAPGAAPTAPAAPASEGSAS
CCCCCEEECCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1781728; 14500908 [H]