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The map label for this gene is rfbB [H]

Identifier: 94311667

GI number: 94311667

Start: 2970411

End: 2971475

Strand: Reverse

Name: rfbB [H]

Synonym: Rmet_2735

Alternate gene names: 94311667

Gene position: 2971475-2970411 (Counterclockwise)

Preceding gene: 94311669

Following gene: 94311666

Centisome position: 75.65

GC content: 60.94

Gene sequence:

>1065_bases
TTGTCGCACATTCTTGTCACAGGCGGAGCGGGGTTCATCGGCGCCAATTTCGTGCTGAACTGGCTGGCCCAGCCAGGCGC
AGACAGCGTCGTAAATGTCGACAAGCTGACATATGCGGGTAACCGCAAGACTCTGGCGTCGCTGGACGGCGATTCCCGTC
ACGTATTCTCGCAAACGGATATCTGCGATCGGGACGCACTTGATCGCTTGCTGGCCGAGCACAAGCCGCGTGCGATCGTG
CATTTCGCCGCGGAGAGCCACGTTGATCGATCCATCCACGGCCCCGCAGAGTTCATCCAGACCAATATCGTCGGCACGTT
CACGCTGCTCGAGGCAGTGCGTGCCTATTGGAACGGCCTGGACGCCGAAGCCAAGGCGGCGTTCCGCTTCCTGCATGTTT
CCACCGACGAGGTATTCGGCTCACTCGGCCCGGCCGATCCGCAGTTCTCCGAGACGACCGCGTACGCGCCGAACAGCCCT
TACTCGGCGTCGAAGGCTGCGTCCGACCATCTCGTCCGCGCCTACCATCACACTTACGGCCTGCCGGTCCTGACGACCAA
CTGCTCCAACAACTACGGTCCGTACCACTTCCCCGAGAAGCTGATCCCGCTGATGATCACCAACGCGCTTTCGGGCAAGC
CGCTGCCAGTCTATGGCGATGGCCAGAACGTGCGCGACTGGCTATACGTCGGGGATCACTGCAGCGCCATTCGCGAGGTG
TTGGCGCGCGGCCGCGTGGGTGAAACGTATAACGTCGGCGGCTGGAACGAAATGACCAACCTGGACGTCGTGCACACAAT
CTGCAGCTTGCTCGATCAACTCAAGCCCAAGGCGGCTGGTTCCTACAGCGACCAGATCAATTTCGTGAAAGACCGGCCCG
GGCACGATCGCCGCTACGCGATCGATGCACGCAAGCTGGAGCGGGAACTGGGTTGGAAGCCGGCCGAAACTTTCGAAAGC
GGACTGCGCAAGACTGTCCAGTGGTACCTGGACAATCAGCCCTGGGTTCAGGACGTGATCTCCGGTGAGTACCGCAACTG
GGTAGCGAAGCAATATGCTGCGTGA

Upstream 100 bases:

>100_bases
GAAAAACTTAAAAATAACGACCGGCTTCCCATGAGGAGAGGGCGGCGCGGCGAGTATTGTTGGGAACGCGGACACACTGA
TAGGTAAAGGGAGTAGAGAT

Downstream 100 bases:

>100_bases
AGCACAGAGAGCCCCCACGCTTCTTGTAACAGGAAGCAATGGCCAGGTCGGCTTCGAACTGCGTCGCAGTCTGGCGCCGC
TGGGCCGGGTCGTGGCGCTG

Product: dTDP-glucose 4,6 dehydratase, NAD(P)-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MSHILVTGGAGFIGANFVLNWLAQPGADSVVNVDKLTYAGNRKTLASLDGDSRHVFSQTDICDRDALDRLLAEHKPRAIV
HFAAESHVDRSIHGPAEFIQTNIVGTFTLLEAVRAYWNGLDAEAKAAFRFLHVSTDEVFGSLGPADPQFSETTAYAPNSP
YSASKAASDHLVRAYHHTYGLPVLTTNCSNNYGPYHFPEKLIPLMITNALSGKPLPVYGDGQNVRDWLYVGDHCSAIREV
LARGRVGETYNVGGWNEMTNLDVVHTICSLLDQLKPKAAGSYSDQINFVKDRPGHDRRYAIDARKLERELGWKPAETFES
GLRKTVQWYLDNQPWVQDVISGEYRNWVAKQYAA

Sequences:

>Translated_354_residues
MSHILVTGGAGFIGANFVLNWLAQPGADSVVNVDKLTYAGNRKTLASLDGDSRHVFSQTDICDRDALDRLLAEHKPRAIV
HFAAESHVDRSIHGPAEFIQTNIVGTFTLLEAVRAYWNGLDAEAKAAFRFLHVSTDEVFGSLGPADPQFSETTAYAPNSP
YSASKAASDHLVRAYHHTYGLPVLTTNCSNNYGPYHFPEKLIPLMITNALSGKPLPVYGDGQNVRDWLYVGDHCSAIREV
LARGRVGETYNVGGWNEMTNLDVVHTICSLLDQLKPKAAGSYSDQINFVKDRPGHDRRYAIDARKLERELGWKPAETFES
GLRKTVQWYLDNQPWVQDVISGEYRNWVAKQYAA
>Mature_353_residues
SHILVTGGAGFIGANFVLNWLAQPGADSVVNVDKLTYAGNRKTLASLDGDSRHVFSQTDICDRDALDRLLAEHKPRAIVH
FAAESHVDRSIHGPAEFIQTNIVGTFTLLEAVRAYWNGLDAEAKAAFRFLHVSTDEVFGSLGPADPQFSETTAYAPNSPY
SASKAASDHLVRAYHHTYGLPVLTTNCSNNYGPYHFPEKLIPLMITNALSGKPLPVYGDGQNVRDWLYVGDHCSAIREVL
ARGRVGETYNVGGWNEMTNLDVVHTICSLLDQLKPKAAGSYSDQINFVKDRPGHDRRYAIDARKLERELGWKPAETFESG
LRKTVQWYLDNQPWVQDVISGEYRNWVAKQYAA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=329, Percent_Identity=34.3465045592705, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI42516563, Length=338, Percent_Identity=26.0355029585799, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI56237023, Length=362, Percent_Identity=25.414364640884, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI56118217, Length=362, Percent_Identity=25.414364640884, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI189083684, Length=362, Percent_Identity=25.414364640884, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1788353, Length=357, Percent_Identity=62.7450980392157, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI48994969, Length=348, Percent_Identity=64.0804597701149, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI1786974, Length=350, Percent_Identity=27.4285714285714, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788366, Length=301, Percent_Identity=25.9136212624585, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788365, Length=254, Percent_Identity=24.8031496062992, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17568069, Length=332, Percent_Identity=36.144578313253, Blast_Score=209, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI115532424, Length=340, Percent_Identity=32.3529411764706, Blast_Score=177, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI71982035, Length=363, Percent_Identity=26.1707988980716, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71982038, Length=363, Percent_Identity=25.6198347107438, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17539532, Length=336, Percent_Identity=24.4047619047619, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17539422, Length=295, Percent_Identity=24.7457627118644, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI133901788, Length=295, Percent_Identity=24.7457627118644, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133901786, Length=295, Percent_Identity=24.7457627118644, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133901790, Length=295, Percent_Identity=24.7457627118644, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=295, Percent_Identity=24.7457627118644, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17507723, Length=285, Percent_Identity=24.9122807017544, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=364, Percent_Identity=26.3736263736264, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19923002, Length=366, Percent_Identity=27.5956284153005, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356223, Length=331, Percent_Identity=25.3776435045317, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24158427, Length=275, Percent_Identity=26.5454545454545, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39323; Mature: 39192

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHILVTGGAGFIGANFVLNWLAQPGADSVVNVDKLTYAGNRKTLASLDGDSRHVFSQTD
CCEEEEECCCCHHHHHHHHHHHCCCCCCCEEEHHHEEECCCCCEEEECCCCCCEEECCCC
ICDRDALDRLLAEHKPRAIVHFAAESHVDRSIHGPAEFIQTNIVGTFTLLEAVRAYWNGL
CCCHHHHHHHHHHCCCCEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
DAEAKAAFRFLHVSTDEVFGSLGPADPQFSETTAYAPNSPYSASKAASDHLVRAYHHTYG
CHHHHHHHHEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
LPVLTTNCSNNYGPYHFPEKLIPLMITNALSGKPLPVYGDGQNVRDWLYVGDHCSAIREV
CCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCHHEEECHHHHHHHHH
LARGRVGETYNVGGWNEMTNLDVVHTICSLLDQLKPKAAGSYSDQINFVKDRPGHDRRYA
HHCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCEEE
IDARKLERELGWKPAETFESGLRKTVQWYLDNQPWVQDVISGEYRNWVAKQYAA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure 
SHILVTGGAGFIGANFVLNWLAQPGADSVVNVDKLTYAGNRKTLASLDGDSRHVFSQTD
CEEEEECCCCHHHHHHHHHHHCCCCCCCEEEHHHEEECCCCCEEEECCCCCCEEECCCC
ICDRDALDRLLAEHKPRAIVHFAAESHVDRSIHGPAEFIQTNIVGTFTLLEAVRAYWNGL
CCCHHHHHHHHHHCCCCEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
DAEAKAAFRFLHVSTDEVFGSLGPADPQFSETTAYAPNSPYSASKAASDHLVRAYHHTYG
CHHHHHHHHEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
LPVLTTNCSNNYGPYHFPEKLIPLMITNALSGKPLPVYGDGQNVRDWLYVGDHCSAIREV
CCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCHHEEECHHHHHHHHH
LARGRVGETYNVGGWNEMTNLDVVHTICSLLDQLKPKAAGSYSDQINFVKDRPGHDRRYA
HHCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCEEE
IDARKLERELGWKPAETFESGLRKTVQWYLDNQPWVQDVISGEYRNWVAKQYAA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]