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The map label for this gene is trmB

Identifier: 94311636

GI number: 94311636

Start: 2937928

End: 2938716

Strand: Direct

Name: trmB

Synonym: Rmet_2704

Alternate gene names: 94311636

Gene position: 2937928-2938716 (Clockwise)

Preceding gene: 94311634

Following gene: 335055625

Centisome position: 74.79

GC content: 64.51

Gene sequence:

>789_bases
ATGCTTCCCCAAGACACGAACACCGATCCGCTGCCGGGCGACGATGCCGAAAGCGCTTCTGGAAAGTCCGCCGACGCCTC
CCAAGGCACCCCCAACCCGGGCGACGAAGTCGCGCATCCGCGCAGAATCCGCTCGTTCGTCAGGCGTGCCGGGCGCACCT
CGACCGGACAGCAGCGGGCGATGGACGAACTGGGACCGACCTATATCGTTCCCTACCAGCCCGAACCGCTGGACTGGACG
GCCACATTCGGACGCGAGGCGCCCTCGATCCTCGAAATCGGATTTGGCATGGGCGAGACGACGGCGCATATCGCCGGCCT
CCGGTCACAGGACAACTTCCTGGGCTGCGAGGTGCATGAACCCGGCGTTGGCGCCCTGCTCAAGCTGATCGGAGAGCGTG
GCCTTCAGAACGTGCGCATCATGCAGCACGACGCCGTCGAAGTCGCCGCGCACATGCTCACCGAGAACTCGCTCGATGGC
GTGCATATCTACTTTCCCGACCCGTGGCACAAGAAGCGCCACAACAAGCGTCGGCTGGTGCAACCGCCACTGGTGAAGCT
GCTGGCCAGCCGGCTCAAGCCGGGGGGCTACATTCACTGCGCGACCGATTGGGAAGAGTACGCCCACCAGATGGTGGAAG
TGCTATCCGGCGAGCCGGCCCTCGAAAACACCTCGAAGGCCGATGGCGGATTCGCGGAACGGCCCGATTACCGGCCCGTG
ACCAAGTTCGAGAAGCGCGGCCTGCGCCTTGGCCATGGCGTGTGGGACGTCGTATTCCGGAAGAAGTAA

Upstream 100 bases:

>100_bases
GGTTCGATTCCCATCGCCCGCTCCACTCTCACTACGCAAGCCGCCTCTGGCACCCGGTCAAAACGATGATCGGCGATCCG
CCCGGCGGCTTTTTGTTGCC

Downstream 100 bases:

>100_bases
GCGGATATCGACCGTCGAATCGCAACAAAGCAAAAGAGGGGCAATCGCCCCTCTTTGCATTTCCGATGCCGGGACAAGCC
CGGCAGCGATCAGGCGTGCC

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MLPQDTNTDPLPGDDAESASGKSADASQGTPNPGDEVAHPRRIRSFVRRAGRTSTGQQRAMDELGPTYIVPYQPEPLDWT
ATFGREAPSILEIGFGMGETTAHIAGLRSQDNFLGCEVHEPGVGALLKLIGERGLQNVRIMQHDAVEVAAHMLTENSLDG
VHIYFPDPWHKKRHNKRRLVQPPLVKLLASRLKPGGYIHCATDWEEYAHQMVEVLSGEPALENTSKADGGFAERPDYRPV
TKFEKRGLRLGHGVWDVVFRKK

Sequences:

>Translated_262_residues
MLPQDTNTDPLPGDDAESASGKSADASQGTPNPGDEVAHPRRIRSFVRRAGRTSTGQQRAMDELGPTYIVPYQPEPLDWT
ATFGREAPSILEIGFGMGETTAHIAGLRSQDNFLGCEVHEPGVGALLKLIGERGLQNVRIMQHDAVEVAAHMLTENSLDG
VHIYFPDPWHKKRHNKRRLVQPPLVKLLASRLKPGGYIHCATDWEEYAHQMVEVLSGEPALENTSKADGGFAERPDYRPV
TKFEKRGLRLGHGVWDVVFRKK
>Mature_262_residues
MLPQDTNTDPLPGDDAESASGKSADASQGTPNPGDEVAHPRRIRSFVRRAGRTSTGQQRAMDELGPTYIVPYQPEPLDWT
ATFGREAPSILEIGFGMGETTAHIAGLRSQDNFLGCEVHEPGVGALLKLIGERGLQNVRIMQHDAVEVAAHMLTENSLDG
VHIYFPDPWHKKRHNKRRLVQPPLVKLLASRLKPGGYIHCATDWEEYAHQMVEVLSGEPALENTSKADGGFAERPDYRPV
TKFEKRGLRLGHGVWDVVFRKK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=228, Percent_Identity=53.5087719298246, Blast_Score=243, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6320000, Length=174, Percent_Identity=25.2873563218391, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_RALME (Q1LJU9)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_584846.1
- ProteinModelPortal:   Q1LJU9
- SMR:   Q1LJU9
- STRING:   Q1LJU9
- GeneID:   4039530
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_2704
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- PhylomeDB:   Q1LJU9
- ProtClustDB:   PRK00121
- BioCyc:   RMET266264:RMET_2704-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 29030; Mature: 29030

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: BINDING 92-92 BINDING 117-117 BINDING 144-144 BINDING 167-167 BINDING 171-171 BINDING 203-203

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPQDTNTDPLPGDDAESASGKSADASQGTPNPGDEVAHPRRIRSFVRRAGRTSTGQQRA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCCCHHHHH
MDELGPTYIVPYQPEPLDWTATFGREAPSILEIGFGMGETTAHIAGLRSQDNFLGCEVHE
HHHCCCEEEECCCCCCCCHHHHCCCCCCCHHHHCCCCCCHHHHHHCCCCCCCEEEEEECC
PGVGALLKLIGERGLQNVRIMQHDAVEVAAHMLTENSLDGVHIYFPDPWHKKRHNKRRLV
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHCCCCCCC
QPPLVKLLASRLKPGGYIHCATDWEEYAHQMVEVLSGEPALENTSKADGGFAERPDYRPV
CCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCH
TKFEKRGLRLGHGVWDVVFRKK
HHHHHCCCCCCCCHHHHHEECC
>Mature Secondary Structure
MLPQDTNTDPLPGDDAESASGKSADASQGTPNPGDEVAHPRRIRSFVRRAGRTSTGQQRA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCCCHHHHH
MDELGPTYIVPYQPEPLDWTATFGREAPSILEIGFGMGETTAHIAGLRSQDNFLGCEVHE
HHHCCCEEEECCCCCCCCHHHHCCCCCCCHHHHCCCCCCHHHHHHCCCCCCCEEEEEECC
PGVGALLKLIGERGLQNVRIMQHDAVEVAAHMLTENSLDGVHIYFPDPWHKKRHNKRRLV
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHCCCCCCC
QPPLVKLLASRLKPGGYIHCATDWEEYAHQMVEVLSGEPALENTSKADGGFAERPDYRPV
CCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCH
TKFEKRGLRLGHGVWDVVFRKK
HHHHHCCCCCCCCHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA