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The map label for this gene is glyA [H]

Identifier: 94311612

GI number: 94311612

Start: 2912883

End: 2914409

Strand: Direct

Name: glyA [H]

Synonym: Rmet_2680

Alternate gene names: 94311612

Gene position: 2912883-2914409 (Clockwise)

Preceding gene: 94311597

Following gene: 94311613

Centisome position: 74.16

GC content: 63.06

Gene sequence:

>1527_bases
TTGCGCGACTGGCGAAGTCAGTGGGTACTACCACGAGGAAGCGCAAGTTCATTGGGCGTGATATTCGATGCGCCTGCCGC
TCGCCTGGGCAGCGAATGGGAAGCAGGGTGCGCCCTGCACCGGCTGGTCGCGTTTTCGCGCCCCCGGTGCTGGACGCCAG
TGCCTTCCCCGCGCGCCCTAATACGCGAAAATCCCGCAATACAGAGTAGCGTCGACAGGCATCCGGTTTTCGTACCGGCC
GTGCCATCCGCTCTCCCTTTTAAACAGACGAGTCACGCCATGTTTGAACGTAGCCGCTTCACGATCGAGCAGATCGACCC
CGAAGTCTTTGCCGCGATCCAGAAGGAAAACCAGCGTCAGGAAGACCACATCGAACTGATCGCTTCCGAGAACTACACCT
CGCCGGCCGTGATGGCCGCCCAGGGTTCGCAGCTCACCAACAAGTACGCCGAAGGCTATCCCGGCAAGCGCTACTACGGC
GGTTGCGAGTACGTGGACGTGGTGGAGCAGCTCGCCATCGACCGCGTGAAGCAACTGTTTGGCGCCGAAGCCGCCAACGT
GCAGCCGAACTCGGGCTCGCAGGCCAACCAGGGCGTGTTCTTCGCCATGCTCAAGCCGGGCGACACGATCATGGGCATGA
GCCTGGCCGAAGGCGGTCACCTGACCCACGGCATGGCGCTGAACATGAGCGGCAAGTGGTTCAACGTGGTCAGCTACGGC
CTGAACGCGCAGGAAGACATCGACTACGACGCGCTTGAAAAGCTGGCCCACGAGAAGAAGCCGAAGCTGATCATCGCCGG
CGCATCGGCTTTCGCGCTGCGCATCGACTTCGAACGCATCGCCAAGGTTGCCAAGGCTGTTGGTGCCTACTTCATGGTGG
ACATGGCCCACTATGCCGGCCTGATCGCCGCCGGTGTGTACCCGAACCCGGTGCCGCACGCCGACTTCGTGACCACCACC
ACCCACAAGAGCCTGCGTGGCCCGCGCGGTGGCGTGATCCTGATGAAGGCCGAGCATGAGAAGGCCATCAACTCGGCCAT
CTTCCCGGGCATCCAGGGTGGTCCGCTGATGCACGTGATTGCCGGCAAGGCCGTGGCCTTCAAGGAAGCGCTGCAGCCGG
ACTTCAAGGCGTATCAGGAACAGGTGGTCAAGAACGCCGCCGCACTGGCCGAAACGCTGATCGCCCGCGGTCTGCGCATC
GTATCGGGCCGTACGGAAAGCCACGTGATGCTGGTGGACCTGCGCGCCAAGAAGATCACCGGCAAGGAAGCCGAGAAGAT
CCTCGGCGACGCCCATATCACGGTGAACAAGAACGCGATCCCGAACGATCCGGAAAAGCCGTTCGTCACCTCGGGCATCC
GCCTGGGCTCGCCGGCCATGACCACGCGCGGCTTCAAGGAAGAAGAAGCCCGCCAGGTGGCCAACCTGATCGCCGACGTG
CTGGACAACCCGCACGATGAGGCCAACATCGCCGCCGTGCGCGAACAGGTTGCCGCACTGACCCGTCGCTTCCCGGTCTA
CGGCTGA

Upstream 100 bases:

>100_bases
CGCCAGGCCTTGCCGGATCAGGGCCGGGCGCACATCCGGGCCGGCCATCGTCTTGCAGCAAGCCGCTTGCTCGCGTAAAC
TCTGCGGCAACCATTCCACC

Downstream 100 bases:

>100_bases
TTCGCCAGTCGATCGCGTTATAGTTAGCGCACACGAACGGCGCTGCCACCTCTGAGGTGGCAGCGCCGTTTCAACAACCA
GCACGACCGCAACAGAAATC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT 1; Serine methylase 1 [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MRDWRSQWVLPRGSASSLGVIFDAPAARLGSEWEAGCALHRLVAFSRPRCWTPVPSPRALIRENPAIQSSVDRHPVFVPA
VPSALPFKQTSHAMFERSRFTIEQIDPEVFAAIQKENQRQEDHIELIASENYTSPAVMAAQGSQLTNKYAEGYPGKRYYG
GCEYVDVVEQLAIDRVKQLFGAEAANVQPNSGSQANQGVFFAMLKPGDTIMGMSLAEGGHLTHGMALNMSGKWFNVVSYG
LNAQEDIDYDALEKLAHEKKPKLIIAGASAFALRIDFERIAKVAKAVGAYFMVDMAHYAGLIAAGVYPNPVPHADFVTTT
THKSLRGPRGGVILMKAEHEKAINSAIFPGIQGGPLMHVIAGKAVAFKEALQPDFKAYQEQVVKNAAALAETLIARGLRI
VSGRTESHVMLVDLRAKKITGKEAEKILGDAHITVNKNAIPNDPEKPFVTSGIRLGSPAMTTRGFKEEEARQVANLIADV
LDNPHDEANIAAVREQVAALTRRFPVYG

Sequences:

>Translated_508_residues
MRDWRSQWVLPRGSASSLGVIFDAPAARLGSEWEAGCALHRLVAFSRPRCWTPVPSPRALIRENPAIQSSVDRHPVFVPA
VPSALPFKQTSHAMFERSRFTIEQIDPEVFAAIQKENQRQEDHIELIASENYTSPAVMAAQGSQLTNKYAEGYPGKRYYG
GCEYVDVVEQLAIDRVKQLFGAEAANVQPNSGSQANQGVFFAMLKPGDTIMGMSLAEGGHLTHGMALNMSGKWFNVVSYG
LNAQEDIDYDALEKLAHEKKPKLIIAGASAFALRIDFERIAKVAKAVGAYFMVDMAHYAGLIAAGVYPNPVPHADFVTTT
THKSLRGPRGGVILMKAEHEKAINSAIFPGIQGGPLMHVIAGKAVAFKEALQPDFKAYQEQVVKNAAALAETLIARGLRI
VSGRTESHVMLVDLRAKKITGKEAEKILGDAHITVNKNAIPNDPEKPFVTSGIRLGSPAMTTRGFKEEEARQVANLIADV
LDNPHDEANIAAVREQVAALTRRFPVYG
>Mature_508_residues
MRDWRSQWVLPRGSASSLGVIFDAPAARLGSEWEAGCALHRLVAFSRPRCWTPVPSPRALIRENPAIQSSVDRHPVFVPA
VPSALPFKQTSHAMFERSRFTIEQIDPEVFAAIQKENQRQEDHIELIASENYTSPAVMAAQGSQLTNKYAEGYPGKRYYG
GCEYVDVVEQLAIDRVKQLFGAEAANVQPNSGSQANQGVFFAMLKPGDTIMGMSLAEGGHLTHGMALNMSGKWFNVVSYG
LNAQEDIDYDALEKLAHEKKPKLIIAGASAFALRIDFERIAKVAKAVGAYFMVDMAHYAGLIAAGVYPNPVPHADFVTTT
THKSLRGPRGGVILMKAEHEKAINSAIFPGIQGGPLMHVIAGKAVAFKEALQPDFKAYQEQVVKNAAALAETLIARGLRI
VSGRTESHVMLVDLRAKKITGKEAEKILGDAHITVNKNAIPNDPEKPFVTSGIRLGSPAMTTRGFKEEEARQVANLIADV
LDNPHDEANIAAVREQVAALTRRFPVYG

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=454, Percent_Identity=42.2907488986784, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI261862352, Length=454, Percent_Identity=42.2907488986784, Blast_Score=343, Evalue=3e-94,
Organism=Homo sapiens, GI261862350, Length=454, Percent_Identity=42.2907488986784, Blast_Score=343, Evalue=3e-94,
Organism=Homo sapiens, GI261862348, Length=454, Percent_Identity=42.2907488986784, Blast_Score=343, Evalue=3e-94,
Organism=Homo sapiens, GI261862346, Length=453, Percent_Identity=41.7218543046358, Blast_Score=335, Evalue=8e-92,
Organism=Homo sapiens, GI22547186, Length=455, Percent_Identity=42.1978021978022, Blast_Score=323, Evalue=3e-88,
Organism=Homo sapiens, GI22547189, Length=440, Percent_Identity=40.2272727272727, Blast_Score=289, Evalue=4e-78,
Organism=Escherichia coli, GI1788902, Length=414, Percent_Identity=62.56038647343, Blast_Score=526, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI25144732, Length=400, Percent_Identity=46.75, Blast_Score=355, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI25144729, Length=400, Percent_Identity=46.75, Blast_Score=355, Evalue=4e-98,
Organism=Saccharomyces cerevisiae, GI6319739, Length=401, Percent_Identity=45.3865336658354, Blast_Score=349, Evalue=7e-97,
Organism=Saccharomyces cerevisiae, GI6323087, Length=397, Percent_Identity=46.095717884131, Blast_Score=334, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24640005, Length=482, Percent_Identity=41.9087136929461, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI221329721, Length=454, Percent_Identity=43.3920704845815, Blast_Score=365, Evalue=1e-101,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 55371; Mature: 55371

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDWRSQWVLPRGSASSLGVIFDAPAARLGSEWEAGCALHRLVAFSRPRCWTPVPSPRAL
CCCCHHCCCCCCCCCCCCEEEEECCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHH
IRENPAIQSSVDRHPVFVPAVPSALPFKQTSHAMFERSRFTIEQIDPEVFAAIQKENQRQ
HHCCCCHHHCCCCCCEECCCCCCCCCCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHCCCC
EDHIELIASENYTSPAVMAAQGSQLTNKYAEGYPGKRYYGGCEYVDVVEQLAIDRVKQLF
CCCEEEEECCCCCCCEEEECCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
GAEAANVQPNSGSQANQGVFFAMLKPGDTIMGMSLAEGGHLTHGMALNMSGKWFNVVSYG
CCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCEEEECCCCEEEHHCCC
LNAQEDIDYDALEKLAHEKKPKLIIAGASAFALRIDFERIAKVAKAVGAYFMVDMAHYAG
CCCCCCCCHHHHHHHHHCCCCCEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
LIAAGVYPNPVPHADFVTTTTHKSLRGPRGGVILMKAEHEKAINSAIFPGIQGGPLMHVI
HHHCCCCCCCCCCCCCEEECCHHHHCCCCCCEEEEECCHHHHHHHHHCCCCCCCCEEEEH
AGKAVAFKEALQPDFKAYQEQVVKNAAALAETLIARGLRIVSGRTESHVMLVDLRAKKIT
HCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEEEECHHHCC
GKEAEKILGDAHITVNKNAIPNDPEKPFVTSGIRLGSPAMTTRGFKEEEARQVANLIADV
CHHHHHHHCCCEEEECCCCCCCCCCCCCEECCEECCCCCHHCCCCCHHHHHHHHHHHHHH
LDNPHDEANIAAVREQVAALTRRFPVYG
HCCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRDWRSQWVLPRGSASSLGVIFDAPAARLGSEWEAGCALHRLVAFSRPRCWTPVPSPRAL
CCCCHHCCCCCCCCCCCCEEEEECCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHH
IRENPAIQSSVDRHPVFVPAVPSALPFKQTSHAMFERSRFTIEQIDPEVFAAIQKENQRQ
HHCCCCHHHCCCCCCEECCCCCCCCCCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHCCCC
EDHIELIASENYTSPAVMAAQGSQLTNKYAEGYPGKRYYGGCEYVDVVEQLAIDRVKQLF
CCCEEEEECCCCCCCEEEECCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
GAEAANVQPNSGSQANQGVFFAMLKPGDTIMGMSLAEGGHLTHGMALNMSGKWFNVVSYG
CCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCEEEECCCCEEEHHCCC
LNAQEDIDYDALEKLAHEKKPKLIIAGASAFALRIDFERIAKVAKAVGAYFMVDMAHYAG
CCCCCCCCHHHHHHHHHCCCCCEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
LIAAGVYPNPVPHADFVTTTTHKSLRGPRGGVILMKAEHEKAINSAIFPGIQGGPLMHVI
HHHCCCCCCCCCCCCCEEECCHHHHCCCCCCEEEEECCHHHHHHHHHCCCCCCCCEEEEH
AGKAVAFKEALQPDFKAYQEQVVKNAAALAETLIARGLRIVSGRTESHVMLVDLRAKKIT
HCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEEEECHHHCC
GKEAEKILGDAHITVNKNAIPNDPEKPFVTSGIRLGSPAMTTRGFKEEEARQVANLIADV
CHHHHHHHCCCEEEECCCCCCCCCCCCCEECCEECCCCCHHCCCCCHHHHHHHHHHHHHH
LDNPHDEANIAAVREQVAALTRRFPVYG
HCCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA