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The map label for this gene is mcrB [H]

Identifier: 94311600

GI number: 94311600

Start: 2902129

End: 2903040

Strand: Reverse

Name: mcrB [H]

Synonym: Rmet_2668

Alternate gene names: 94311600

Gene position: 2903040-2902129 (Counterclockwise)

Preceding gene: 94311601

Following gene: 94311599

Centisome position: 73.9

GC content: 49.56

Gene sequence:

>912_bases
ATGCTGGAAAAACTTGAAAAAGTTCGCTCTTTAAGCGCAGCTGAGATGGGATTCGCATCTCCAGCGAATTCTGAGGCTGA
AGCCGATGCGCTAAATAGTAGTGTGACGGGGCCAGAGTTGGATGAGAGCGACGAGAAATATCTCCTTACAGAGGCTTTGC
TGGCTGATGGCTTTGCTGGCGTCGTTTTTAGTGGGCCTCCTGGTACCGGGAAAAGTCGCTATGCACGTGAAATTGCGAGG
CTGTTTGTAGAAGACGACGAGGCTCGAGTATTCTATGTGCAATTTCACCCCGGTTATCAGTACGAAGACTTTATGGAGTC
GTTTGTACCGACAAGCAGTGGAGGATTTGCGCCTAAAGACAAGGTGTTTCTTCAGGCTTGCCGTAGAGCCAGCGAGGTCG
ATGGCCCGGTCGTACTCGTTATTGATGAGTTGAGTCGAACGGATGTGGTACGCGTATTCGGAGAGGCACTTACGTACCTC
GAAAAATCCAAGCGGGGGTTGAGCTTCCAGCTTGCATCTGGACGTACTACATCAATTCCTAATAATCTCATTATGCTAGC
GACCATGAATCCGTGGGATCGTGGCGTGGAGGAAATGGATCTGGCATTGGAGAGGCGATTTGCCCGAATCTCGATTGAGC
CAGATCCGCAAGCGCTTGAGATCATTCTTTCTCGCTCAGCCTTAACCGAAGAGCGTCGATTGCGAGTGATGCGTTTTTTT
CAGACGCTGTATCGGCACGTCAATCCACTCTGTCGAATCGGTCATGCATATTTTCTCACTGCACAAAACGATGACTCGTT
GAAGCGACTTTGGGACAATCAGCTTTCGTTTCATTTCGAGAGGATTTTGAAACGTAGTCCAGAGGAGTTGGAGCGAATTC
GAAACGGGTGGCAGGCTATCATGGCTGAATAG

Upstream 100 bases:

>100_bases
CAGAAGAACGAAGAGGTTGAGCAATTTAATGCCCTTTTCATCGAATCGCACGAAATTTTGAGCAATATGGAAGATATTTT
CTCCCCGTTGGCAGGCTGAT

Downstream 100 bases:

>100_bases
TCATGAGCAGCGTTACTACTTTAATCGTTAAGGAATACGAGACCATCGATATTCCGGCTGCGCTGATCATTGATGGAATT
GCTCTCCGCATCTATCCGGC

Product: hypothetical protein

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MLEKLEKVRSLSAAEMGFASPANSEAEADALNSSVTGPELDESDEKYLLTEALLADGFAGVVFSGPPGTGKSRYAREIAR
LFVEDDEARVFYVQFHPGYQYEDFMESFVPTSSGGFAPKDKVFLQACRRASEVDGPVVLVIDELSRTDVVRVFGEALTYL
EKSKRGLSFQLASGRTTSIPNNLIMLATMNPWDRGVEEMDLALERRFARISIEPDPQALEIILSRSALTEERRLRVMRFF
QTLYRHVNPLCRIGHAYFLTAQNDDSLKRLWDNQLSFHFERILKRSPEELERIRNGWQAIMAE

Sequences:

>Translated_303_residues
MLEKLEKVRSLSAAEMGFASPANSEAEADALNSSVTGPELDESDEKYLLTEALLADGFAGVVFSGPPGTGKSRYAREIAR
LFVEDDEARVFYVQFHPGYQYEDFMESFVPTSSGGFAPKDKVFLQACRRASEVDGPVVLVIDELSRTDVVRVFGEALTYL
EKSKRGLSFQLASGRTTSIPNNLIMLATMNPWDRGVEEMDLALERRFARISIEPDPQALEIILSRSALTEERRLRVMRFF
QTLYRHVNPLCRIGHAYFLTAQNDDSLKRLWDNQLSFHFERILKRSPEELERIRNGWQAIMAE
>Mature_303_residues
MLEKLEKVRSLSAAEMGFASPANSEAEADALNSSVTGPELDESDEKYLLTEALLADGFAGVVFSGPPGTGKSRYAREIAR
LFVEDDEARVFYVQFHPGYQYEDFMESFVPTSSGGFAPKDKVFLQACRRASEVDGPVVLVIDELSRTDVVRVFGEALTYL
EKSKRGLSFQLASGRTTSIPNNLIMLATMNPWDRGVEEMDLALERRFARISIEPDPQALEIILSRSALTEERRLRVMRFF
QTLYRHVNPLCRIGHAYFLTAQNDDSLKRLWDNQLSFHFERILKRSPEELERIRNGWQAIMAE

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=234, Percent_Identity=32.9059829059829, Blast_Score=117, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 34372; Mature: 34372

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEKLEKVRSLSAAEMGFASPANSEAEADALNSSVTGPELDESDEKYLLTEALLADGFAG
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
VVFSGPPGTGKSRYAREIARLFVEDDEARVFYVQFHPGYQYEDFMESFVPTSSGGFAPKD
EEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCHH
KVFLQACRRASEVDGPVVLVIDELSRTDVVRVFGEALTYLEKSKRGLSFQLASGRTTSIP
HHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
NNLIMLATMNPWDRGVEEMDLALERRFARISIEPDPQALEIILSRSALTEERRLRVMRFF
CCEEEEEECCHHHHCHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QTLYRHVNPLCRIGHAYFLTAQNDDSLKRLWDNQLSFHFERILKRSPEELERIRNGWQAI
HHHHHHHCHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
MAE
HCC
>Mature Secondary Structure
MLEKLEKVRSLSAAEMGFASPANSEAEADALNSSVTGPELDESDEKYLLTEALLADGFAG
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
VVFSGPPGTGKSRYAREIARLFVEDDEARVFYVQFHPGYQYEDFMESFVPTSSGGFAPKD
EEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCHH
KVFLQACRRASEVDGPVVLVIDELSRTDVVRVFGEALTYLEKSKRGLSFQLASGRTTSIP
HHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
NNLIMLATMNPWDRGVEEMDLALERRFARISIEPDPQALEIILSRSALTEERRLRVMRFF
CCEEEEEECCHHHHCHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QTLYRHVNPLCRIGHAYFLTAQNDDSLKRLWDNQLSFHFERILKRSPEELERIRNGWQAI
HHHHHHHCHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
MAE
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]