Click here to switch to the map view.

The map label for this gene is pmbA [H]

Identifier: 94311506

GI number: 94311506

Start: 2795043

End: 2796410

Strand: Reverse

Name: pmbA [H]

Synonym: Rmet_2570

Alternate gene names: 94311506

Gene position: 2796410-2795043 (Counterclockwise)

Preceding gene: 94311509

Following gene: 94311502

Centisome position: 71.19

GC content: 65.79

Gene sequence:

>1368_bases
ATGAGCCAGACCGCTGAACAGACCGCGCATTTCACCTACACCCAGGACCAGCTCAAGGCCATGGCGGCCGATGTGCTGCG
TGTGGCACGCGAGCTCGGCGCGACGGATGCCGCCACCGAGATTTCCGAGGGCAGTGGCCTGTCGGTGTCGGTGCGCAAGG
GCGAGGTGGAAACCATCGAGCAGAATCGCGACAAGGTGGTGGGTGTGACGGTGATGATCGGCAAGCGTCGCGGCAATGCC
AGCACGTCAGATTTTTCGGCCGCCGCGCTGCGTGCCACGGCCGAAGCCGCATACAACATCGCCCGTTTCACGGCCGAGGA
TGACTGCGCCGGCCTGGCCGAGGAAGAGTTGCTGGAGCGCGACCCCCAGGATCTGGACCTGTTCCACCCGTGGACCATCG
ATGCCGAAGGCGCGATCGACATTGCCCGCCGCGCCGAAGCGGCGGCTTTCGCCGTCTCGCCGAAGATTCGCAACAGCGAC
GGCGCTAGCGTGTCGGCCCAGCACTCGCAGTTCGTGCTGGCCACGTCGCGCGGCTTCTCGGGCGGCTATCCCTACTCTCG
CCACTTCATCTCGTGTGCGCCAATCGCCGGTTCAGGCGGCGGCATGCAGCGGGATGACTGGTATTCGTCGAAGCGCGCTC
CCGGAGCGCTGGCTGAGCCGGAAGCGATTGGCCGCTATGCGGCGGAGCGCGCGCTGGCCCGCCTGAACGCCCGCAAGCTG
TCCACGCGCAAGTGCCCGGTGCTGTTCGAGGCGCCACTGGCTGCTGGCCTGCTAGGCGCGTTCGTGCAGGCGGTGTCGGG
TGGCGCGCTGTATCGCAAGTCGACGTTCCTGTATGACACGCTGGGCAAGGAAGTTTTCGCGCCGCACATCCAGGTTTACG
AACGGCCGCACGTGCCGGGCGCGATGGGCAGCGCGCCGTTCGACGAAGAGGGCGTGCGCACGCACGAACGCGACGTGGTG
CGCGACGGCGTGGTGCAGGGCTACTTCCTGTCGACGTACTCGGCACGCAAGCTCGGCATGAAGACTACCGGCAACGCGGG
CGGCTCGCACAACCTCACGCTGACCAGCTCGCAGACGCAAGCGGGCGACGATTTCGTCGGGATGCTGAAGAAGATGGGCA
CCGGTCTGCTGGTGACCGAGCTGATGGGGCAGGGCGTGAACTACGTTACCGGCGACTATTCGCGCGGCGCCTCGGGCTAC
TGGGTCGAAAACGGCGTGATCCAGTATCCGGTGGAGGAAATCACGATTGCCGGCAACATGGTCGAGATGCTGCGCCAGAT
CGTTGCTGTTGGTGCCGATGCGCTGGTGCGTGGCACCAAGGAGACTGGCTCGATCCTGATCGAGCAGATGACGATCGCCG
GGAACTGA

Upstream 100 bases:

>100_bases
CCGGGAATCGGCCATTGGGATTGTTACGGGTATTTCGCGTCATGGGCGGCATTTTATCTTGCTATGATTCCCGTTTGGAC
TTTTGATCACGCCCGCCCAT

Downstream 100 bases:

>100_bases
TGGTTTGTGCGTCTCTTCCAAACGGGAGAGACGACAAGGGCCGATCAGGGACGACGAAGGGCGGCGCAAGCGGGTGAACC
CGTTTGCGCCGCCCTTTTTT

Product: peptidase required for the maturation and secretion of the antibiotic peptide MccB17

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MSQTAEQTAHFTYTQDQLKAMAADVLRVARELGATDAATEISEGSGLSVSVRKGEVETIEQNRDKVVGVTVMIGKRRGNA
STSDFSAAALRATAEAAYNIARFTAEDDCAGLAEEELLERDPQDLDLFHPWTIDAEGAIDIARRAEAAAFAVSPKIRNSD
GASVSAQHSQFVLATSRGFSGGYPYSRHFISCAPIAGSGGGMQRDDWYSSKRAPGALAEPEAIGRYAAERALARLNARKL
STRKCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLYDTLGKEVFAPHIQVYERPHVPGAMGSAPFDEEGVRTHERDVV
RDGVVQGYFLSTYSARKLGMKTTGNAGGSHNLTLTSSQTQAGDDFVGMLKKMGTGLLVTELMGQGVNYVTGDYSRGASGY
WVENGVIQYPVEEITIAGNMVEMLRQIVAVGADALVRGTKETGSILIEQMTIAGN

Sequences:

>Translated_455_residues
MSQTAEQTAHFTYTQDQLKAMAADVLRVARELGATDAATEISEGSGLSVSVRKGEVETIEQNRDKVVGVTVMIGKRRGNA
STSDFSAAALRATAEAAYNIARFTAEDDCAGLAEEELLERDPQDLDLFHPWTIDAEGAIDIARRAEAAAFAVSPKIRNSD
GASVSAQHSQFVLATSRGFSGGYPYSRHFISCAPIAGSGGGMQRDDWYSSKRAPGALAEPEAIGRYAAERALARLNARKL
STRKCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLYDTLGKEVFAPHIQVYERPHVPGAMGSAPFDEEGVRTHERDVV
RDGVVQGYFLSTYSARKLGMKTTGNAGGSHNLTLTSSQTQAGDDFVGMLKKMGTGLLVTELMGQGVNYVTGDYSRGASGY
WVENGVIQYPVEEITIAGNMVEMLRQIVAVGADALVRGTKETGSILIEQMTIAGN
>Mature_454_residues
SQTAEQTAHFTYTQDQLKAMAADVLRVARELGATDAATEISEGSGLSVSVRKGEVETIEQNRDKVVGVTVMIGKRRGNAS
TSDFSAAALRATAEAAYNIARFTAEDDCAGLAEEELLERDPQDLDLFHPWTIDAEGAIDIARRAEAAAFAVSPKIRNSDG
ASVSAQHSQFVLATSRGFSGGYPYSRHFISCAPIAGSGGGMQRDDWYSSKRAPGALAEPEAIGRYAAERALARLNARKLS
TRKCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLYDTLGKEVFAPHIQVYERPHVPGAMGSAPFDEEGVRTHERDVVR
DGVVQGYFLSTYSARKLGMKTTGNAGGSHNLTLTSSQTQAGDDFVGMLKKMGTGLLVTELMGQGVNYVTGDYSRGASGYW
VENGVIQYPVEEITIAGNMVEMLRQIVAVGADALVRGTKETGSILIEQMTIAGN

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=424, Percent_Identity=48.8207547169811, Blast_Score=407, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48541; Mature: 48410

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTAEQTAHFTYTQDQLKAMAADVLRVARELGATDAATEISEGSGLSVSVRKGEVETIE
CCCCHHHHHEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEECCCHHHHH
QNRDKVVGVTVMIGKRRGNASTSDFSAAALRATAEAAYNIARFTAEDDCAGLAEEELLER
CCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHC
DPQDLDLFHPWTIDAEGAIDIARRAEAAAFAVSPKIRNSDGASVSAQHSQFVLATSRGFS
CCCCCCCCCCEECCCCCHHHHHHHHHHHEEEECCCCCCCCCCCEECCCCEEEEEECCCCC
GGYPYSRHFISCAPIAGSGGGMQRDDWYSSKRAPGALAEPEAIGRYAAERALARLNARKL
CCCCCCCCEEEEECCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
STRKCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLYDTLGKEVFAPHIQVYERPHVPG
CCCCCCEEEECHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCCHHEEECCCCCC
AMGSAPFDEEGVRTHERDVVRDGVVQGYFLSTYSARKLGMKTTGNAGGSHNLTLTSSQTQ
CCCCCCCCCCCCCHHHHHHHHHHHHCCEEEECHHHHHCCCCCCCCCCCCCCEEEECCCCC
AGDDFVGMLKKMGTGLLVTELMGQGVNYVTGDYSRGASGYWVENGVIQYPVEEITIAGNM
CCHHHHHHHHHHCCCHHHHHHHHCCCCEEECCCCCCCCCEEEECCEEECCHHHEEECCHH
VEMLRQIVAVGADALVRGTKETGSILIEQMTIAGN
HHHHHHHHHHCCHHHHCCCCHHHHEEEEEEEECCC
>Mature Secondary Structure 
SQTAEQTAHFTYTQDQLKAMAADVLRVARELGATDAATEISEGSGLSVSVRKGEVETIE
CCCHHHHHEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEECCCHHHHH
QNRDKVVGVTVMIGKRRGNASTSDFSAAALRATAEAAYNIARFTAEDDCAGLAEEELLER
CCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHC
DPQDLDLFHPWTIDAEGAIDIARRAEAAAFAVSPKIRNSDGASVSAQHSQFVLATSRGFS
CCCCCCCCCCEECCCCCHHHHHHHHHHHEEEECCCCCCCCCCCEECCCCEEEEEECCCCC
GGYPYSRHFISCAPIAGSGGGMQRDDWYSSKRAPGALAEPEAIGRYAAERALARLNARKL
CCCCCCCCEEEEECCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
STRKCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLYDTLGKEVFAPHIQVYERPHVPG
CCCCCCEEEECHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCCHHEEECCCCCC
AMGSAPFDEEGVRTHERDVVRDGVVQGYFLSTYSARKLGMKTTGNAGGSHNLTLTSSQTQ
CCCCCCCCCCCCCHHHHHHHHHHHHCCEEEECHHHHHCCCCCCCCCCCCCCEEEECCCCC
AGDDFVGMLKKMGTGLLVTELMGQGVNYVTGDYSRGASGYWVENGVIQYPVEEITIAGNM
CCHHHHHHHHHHCCCHHHHHHHHCCCCEEECCCCCCCCCEEEECCEEECCHHHEEECCHH
VEMLRQIVAVGADALVRGTKETGSILIEQMTIAGN
HHHHHHHHHHCCHHHHCCCCHHHHEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]