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The map label for this gene is tnpB [C]

Identifier: 94311492

GI number: 94311492

Start: 2780001

End: 2780837

Strand: Reverse

Name: tnpB [C]

Synonym: Rmet_2556

Alternate gene names: 94311492

Gene position: 2780837-2780001 (Counterclockwise)

Preceding gene: 94311493

Following gene: 94311491

Centisome position: 70.79

GC content: 61.53

Gene sequence:

>837_bases
ATGAAGACGGTCTGCGATGTCCTTGGCGTGGCGCGCTCTGCGGTGGCAGCGAAACAGGCCCGTTCGTCGGAATGGCGGGA
TGGCCGCCGCGCCCGCCAATACGACGACACGGCGCTGGTGGCTGAAATCCATGAGCTGGTTGCGGGCCTGCCGACATACG
GCTACCGAAGGGTCTGGGCTTTGCTACGGCGCAGTCATGAGCTGAGCGGGGCCTCGCCCGTGAACGCAAAGCGTGTTTAT
CGTGTGATGCACGACCACCAGTTGCTGCTTCGCCGGCCTGGGCGACGACTCGATACACGTCGCCATGACGGTCGGGTCGC
TGTCGACCGTAGCAATACCCGCTGGTGTTCAGACGGCTTTGAATTCCGTTGCGACGATGGTTCGCCGCTACGCGTCACCT
TTGCGCTCGATTGCCATGACCGAGAAGCCATTAGTTGGGCGGCGACCACCGGCGGGCACAGTGGGGATATTGTTCGAGAT
GTCATGCTGGCGGCCGTCGAGCAGCGCTTTGGCGCCGTGCAGACCGAGCAAACCATCGAATGGCTCTCGGACAACGGCTC
GGCGTACATCGACCATCGTACGCGCAGCTTCGCGCGGGAACTGGGCCTGGAGCCACTGACCACGCCGGTGCGTTCTCCGC
AGAGCAACGGTATGGCCGAATCCTTCGTCAAGACCATGAAGCGCGATTACATCGCGTTCATGAACAAGCCCGACGTGCCG
ACCGCGCTCTCTCAGCTAACGGTCGCGTTCGAGCAATACAATGACTGGCATCCGCATAAAGCGTTGAAGTATCGTTCGCC
TCGGGAGTTCCGACGCGCCGCAACATCATCAACTTAA

Upstream 100 bases:

>100_bases
GAAAGAAAACGCAGGAAGCCGAGATCCTGAAGGAAGCCGTGGAGTACGGGCGCTCAAAAAACTGGATTGCGCGCTCGCCC
TTACTGCCCGGGGACGACCA

Downstream 100 bases:

>100_bases
CGGTGTTCGGGTGTCCTGAGTTACAGGGTCAACTCCAAATGGTTCGAGCGGACGTTACTGCTTAATGGCGAGGAGATGGG
TGCTTTGATCCTTCTCGAAA

Product: transposase IS1087B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MKTVCDVLGVARSAVAAKQARSSEWRDGRRARQYDDTALVAEIHELVAGLPTYGYRRVWALLRRSHELSGASPVNAKRVY
RVMHDHQLLLRRPGRRLDTRRHDGRVAVDRSNTRWCSDGFEFRCDDGSPLRVTFALDCHDREAISWAATTGGHSGDIVRD
VMLAAVEQRFGAVQTEQTIEWLSDNGSAYIDHRTRSFARELGLEPLTTPVRSPQSNGMAESFVKTMKRDYIAFMNKPDVP
TALSQLTVAFEQYNDWHPHKALKYRSPREFRRAATSST

Sequences:

>Translated_278_residues
MKTVCDVLGVARSAVAAKQARSSEWRDGRRARQYDDTALVAEIHELVAGLPTYGYRRVWALLRRSHELSGASPVNAKRVY
RVMHDHQLLLRRPGRRLDTRRHDGRVAVDRSNTRWCSDGFEFRCDDGSPLRVTFALDCHDREAISWAATTGGHSGDIVRD
VMLAAVEQRFGAVQTEQTIEWLSDNGSAYIDHRTRSFARELGLEPLTTPVRSPQSNGMAESFVKTMKRDYIAFMNKPDVP
TALSQLTVAFEQYNDWHPHKALKYRSPREFRRAATSST
>Mature_278_residues
MKTVCDVLGVARSAVAAKQARSSEWRDGRRARQYDDTALVAEIHELVAGLPTYGYRRVWALLRRSHELSGASPVNAKRVY
RVMHDHQLLLRRPGRRLDTRRHDGRVAVDRSNTRWCSDGFEFRCDDGSPLRVTFALDCHDREAISWAATTGGHSGDIVRD
VMLAAVEQRFGAVQTEQTIEWLSDNGSAYIDHRTRSFARELGLEPLTTPVRSPQSNGMAESFVKTMKRDYIAFMNKPDVP
TALSQLTVAFEQYNDWHPHKALKYRSPREFRRAATSST

Specific function: Involved in the transposition of the insertion sequence is2 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1790724, Length=270, Percent_Identity=59.6296296296296, Blast_Score=324, Evalue=4e-90,
Organism=Escherichia coli, GI1789424, Length=270, Percent_Identity=59.6296296296296, Blast_Score=324, Evalue=4e-90,
Organism=Escherichia coli, GI1789224, Length=270, Percent_Identity=59.6296296296296, Blast_Score=324, Evalue=4e-90,
Organism=Escherichia coli, GI1788305, Length=270, Percent_Identity=59.6296296296296, Blast_Score=324, Evalue=4e-90,
Organism=Escherichia coli, GI1787669, Length=270, Percent_Identity=59.6296296296296, Blast_Score=324, Evalue=4e-90,
Organism=Escherichia coli, GI1786558, Length=270, Percent_Identity=59.6296296296296, Blast_Score=324, Evalue=4e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR002514 [H]

Pfam domain/function: PF00665 rve; PF01527 Transposase_8 [H]

EC number: NA

Molecular weight: Translated: 31604; Mature: 31604

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVCDVLGVARSAVAAKQARSSEWRDGRRARQYDDTALVAEIHELVAGLPTYGYRRVWA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHH
LLRRSHELSGASPVNAKRVYRVMHDHQLLLRRPGRRLDTRRHDGRVAVDRSNTRWCSDGF
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCEEEEECCCCCEECCCC
EFRCDDGSPLRVTFALDCHDREAISWAATTGGHSGDIVRDVMLAAVEQRFGAVQTEQTIE
EEEECCCCCEEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
WLSDNGSAYIDHRTRSFARELGLEPLTTPVRSPQSNGMAESFVKTMKRDYIAFMNKPDVP
HHHCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCH
TALSQLTVAFEQYNDWHPHKALKYRSPREFRRAATSST
HHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MKTVCDVLGVARSAVAAKQARSSEWRDGRRARQYDDTALVAEIHELVAGLPTYGYRRVWA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHH
LLRRSHELSGASPVNAKRVYRVMHDHQLLLRRPGRRLDTRRHDGRVAVDRSNTRWCSDGF
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCEEEEECCCCCEECCCC
EFRCDDGSPLRVTFALDCHDREAISWAATTGGHSGDIVRDVMLAAVEQRFGAVQTEQTIE
EEEECCCCCEEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
WLSDNGSAYIDHRTRSFARELGLEPLTTPVRSPQSNGMAESFVKTMKRDYIAFMNKPDVP
HHHCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCH
TALSQLTVAFEQYNDWHPHKALKYRSPREFRRAATSST
HHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA